CN109123102A - A kind of aseptic experiment mouse feed and preparation method thereof - Google Patents
A kind of aseptic experiment mouse feed and preparation method thereof Download PDFInfo
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- CN109123102A CN109123102A CN201811133962.9A CN201811133962A CN109123102A CN 109123102 A CN109123102 A CN 109123102A CN 201811133962 A CN201811133962 A CN 201811133962A CN 109123102 A CN109123102 A CN 109123102A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
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Abstract
The present invention relates to the experimental animal field of feed of biological medicine, provide a kind of feed suitable for aseptic experiment mouse, comprising: 14-28IU/g vitamin A, 1.5-4.3IU/g vitamin D, 120-240IU/g vitamin E, 5-20mg/kg Vitamin K3,13-26mg/kg vitamin B1,12-24mg/kg vitamin B2,12-24mg/kg vitamin B6,60-120mg/kg niacin, 24-48mg/kg pantothenic acid, 4-8mg/kg folic acid, 0.2-0.4mg/kg biotin, 0.03-0.06mg/kg vitamin B12.Nutritional ingredient in the method for preparing above-mentioned feed, including detection base grain, adds nutriment and granulation.The aseptic experiment mouse feed is conducive to the growth of aseptic experiment mouse and is conducive to quick healing of cut.
Description
Technical field
The present invention relates to the experimental animal field of feed of biological medicine, in particular to a kind of aseptic experiment mouse
Feed and preparation method thereof.
Background technique
So-called germfree animal just refers to the animal of the microorganism and helminth that cannot detect any work.From microbiological
Viewpoint sees that usual experimental animal has helminth in vivo and in vitro, also often has bacterium and virus in vivo, but also be all difficult to
Exclude certain potential infectious diseases.In addition, containing antibody in the serum of routine experimentation animal.So with and deposit conventional animal and carry out
Medical scientific, it will there are various interference, experimental result is often inaccurate.Making experiment using germfree animal can
To overcome disadvantage present in routine experimentation, keep experimental result correctly reliable.Germfree animal is cut open the belly and takes in sterile barrier system
Fetus out, raising breeding in Sterilized isolator, feed, drinking-water by disinfection, periodic inspection, it was demonstrated that inside and outside animal body none
Cut the animal of microorganism and helminth (including most of virus).Therefore, feed needed for germfree animal should adapt to sterile move
What growth, physiological property and the experiment of object needed.
In consideration of it, special propose the application.
Summary of the invention
The present invention provides a kind of aseptic experiment mouse feeds, it is desirable to provide is conducive to the growth of aseptic experiment mouse and is conducive to nothing
The feed of bacterium experimental mouse quick healing of cut.
The present invention provides a kind of preparation method of aseptic experiment mouse feed, it can be made and be conducive to the life of aseptic experiment mouse health
It is long, and be conducive to the aseptic experiment mouse feed of aseptic experiment mouse quick healing of cut.
The present invention is implemented as follows:
A kind of aseptic experiment mouse feed, aseptic experiment mouse feed include:
14-28IU/g vitamin A, 1.5-4.3IU/g vitamin D, 120-240IU/g vitamin E, 5-20mg/kg dimension life
Plain K3,13-26mg/kg vitamin B1,12-24mg/kg vitamin B2,12-24mg/kg vitamin B6,60-120mg/kg cigarette
Acid, 24-48mg/kg pantothenic acid, 4-8mg/kg folic acid, 0.2-0.4mg/kg biotin, 0.03-0.06mg/kg vitamin B12.
A kind of preparation method of aseptic experiment mouse feed, comprising:
Each nutrient composition content in experimental animal base grain made from detection in advance；
Using following nutrition content as aseptic experiment mouse feed nutrition content standard:
14-28IU/g vitamin A, 1.5-4.3IU/g vitamin D, 120-240IU/g vitamin E, 5-20mg/kg dimension life
Plain K3,13-26mg/kg vitamin B1,12-24mg/kg vitamin B2,12-24mg/kg vitamin B6,60-120mg/kg cigarette
Acid, 24-48mg/kg pantothenic acid, 4-8mg/kg folic acid, 0.2-0.4mg/kg biotin, 0.03-0.06mg/kg vitamin B12；
By aseptic experiment mouse feed nutrition content standard, nutriment is added into experimental animal base grain, primary is made
Aseptic experiment mouse feed；
Above-mentioned aseptic experiment mouse feed is made in primary aseptic experiment mouse feed after pelletizing.
The beneficial effects of the present invention are: the aseptic experiment mouse feed that the present invention obtains by above-mentioned design, containing sufficiently and
Vitamin needed for comprehensive various aseptic experiment mouse, enables to aseptic experiment mouse to grow up healthy and sound, especially containing abundant
B family vitamin and vitamin K, can be avoided germfree mouse since itself without microorganism can not synthesize vitamin and influence reality
The normal physiological activity for testing mouse can be improved the resistance of mouse, especially effectively can also avoid germfree mouse after testing
Wound is not easy the phenomenon that healing.
Rationally, nothing provided by the invention can be made in aseptic experiment mouse feed provided by the invention, production process setting
Bacterium tests mouse feed.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
A kind of aseptic experiment mouse feed and preparation method thereof is provided to the embodiment of the present invention below to be specifically described.
A kind of aseptic experiment mouse feed, aseptic experiment mouse feed include:
14-28IU/g vitamin A, 1.5-4.3IU/g vitamin D, 120-240IU/g vitamin E, 5-20mg/kg dimension life
Plain K3,13-26mg/kg vitamin B1,12-24mg/kg vitamin B2,12-24mg/kg vitamin B6,60-120mg/kg cigarette
Acid, 24-48mg/kg pantothenic acid, 4-8mg/kg folic acid, 0.2-0.4mg/kg biotin, 0.03-0.06mg/kg vitamin B12.
Germfree animal is vulnerable, thus germfree animal is higher to B family vitamin requirement, and vitamin K is four kinds of blood coagulations
Albumen (factor, convertion, antihemophilic factor and factor X) synthesizes essential substance in liver,
And germfree animal is used to test often that wound there is no Escherichia coli to synthesize vitamin K, therefore aseptic experiment in vivo in addition
It is needed in mouse feed containing enough vitamin Ks.And due to there's almost no microorganism in germfree animal body, cause a variety of dimensions to give birth to
Element can not synthesize, therefore vitamin rich in is needed in aseptic experiment mouse feed.
In aseptic experiment mouse feed, there are also when mentioned kind and the vitamin of content, aseptic experiment mouse can satisfy just
Needed for normal physiological activity, the healthier growth of aseptic experiment mouse can be made and survival period is more long.
Further, since, almost without microorganism, therefore its immunity is not high in germfree animal body, in order to can be more strong
Kang Shengchang further includes 0.2-0.4mg/kg organic selenium in aseptic experiment mouse feed.The organic selenium is mainly selenomethionine.
Similarly, in order to further increase the immunity of germfree animal, aseptic experiment mouse feed further include: 60-120mg/
Kg organic zinc.The organic zinc added into aseptic experiment mouse feed can be glycine zine.
A kind of preparation method of aseptic experiment mouse feed, comprising:
Each nutrient composition content in experimental animal base grain made from S1, detection in advance.
Firstly, the raw material for preparing that common mouse grain can be used prepares experimental animal base grain.Such as it is optional according to parts by weight
With: 40-43 portions of corn flour, 10-27 parts of wheat brans, 28-30 parts of dregs of beans, 0.1-0.3 portions of salt, 0.9-1.1 parts of bone meal and 0.9-
Manufactured experimental animal base grain is mixed evenly in 1.1 parts of lysines.Beans herein refer to remaining after soybean is squeezed grease
By-product.Experimental animal base grain can also be and directly buy from market.
Then the nutritional ingredient of experimental animal base grain made from detection in advance.
S2, using following nutrition content as aseptic experiment mouse feed nutrition content standard:
14-28IU/g vitamin A, 1.5-4.3IU/g vitamin D, 120-240IU/g vitamin E, 5-20mg/kg dimension life
Plain K3,13-26mg/kg vitamin B1,12-24mg/kg vitamin B2,12-24mg/kg vitamin B6,60-120mg/kg cigarette
Acid, 24-48mg/kg pantothenic acid, 4-8mg/kg folic acid, 0.2-0.4mg/kg biotin, 0.03-0.06mg/kg vitamin B12；
Each nutrient composition content and aseptic experiment mouse feed nutrition content standard in comparative experiments animal base grain, if real
It tests a certain item of animal base grain or several nutriment ingredients is equal to or higher than aseptic experiment mouse feed nutrition content standard then
Without adding corresponding nutriment into experimental animal base grain, if a certain item of experimental animal base grain or several nutriment ingredients
Corresponding nutriment is then added into experimental animal base grain lower than aseptic experiment mouse feed nutrition content standard, primary is made
Aseptic experiment mouse feed.
Due to needing to carry out sterilization treatment to aseptic experiment mouse feed before finished product, and drying course temperature of pelletizing
It is higher, the case where B family vitamin is likely to occur loss at high temperature, to guarantee nutrition in final aseptic experiment mouse feed obtained
Ingredient is in the range of standard requirements.Therefore nutriment is added into experimental animal base grain, and primary aseptic experiment mouse feed is made
When, so that B family vitamin content is higher than the 3- of B family vitamin content in aseptic experiment mouse feed in primary aseptic experiment mouse feed
5%.
Preferably, excessive excess is not being used to add mode of the B family vitamin into primary aseptic experiment mouse feed to protect
When vitamin content is in the range of requiring in aseptic experiment mouse feed made from demonstrate,proving.It, can be with to prevent B family vitamin to be lost
Using the B group family vitamin in the nutriment in the nutriment added into experimental animal base grain by addition using dimension life
It is added to again after plain micro-capsule coating in experimental animal base grain, the additive amount of B family vitamin is sought according to aseptic experiment mouse feed at this time
Feeding standard is estimated vitamin B loss amount again and is calculated to require supplementation with the amount of loss some vitamin.Using vitamin micro-capsule coating
Mode can effectively prevent vitamin high temperature lose.
S3, above-mentioned aseptic experiment mouse feed is made in primary aseptic experiment mouse feed after pelletizing.
Primary aseptic experiment mouse feed is sent into granulator, aseptic experiment mouse provided by the invention is obtained through granulation and raises
Material.
Preferably, aseptic experiment mouse feed finished product obtained need to contain bacterium by sterilization treatment to avoid it.
Specifically, sterilization treatment can be in test experience animal base grain before each nutrient composition content to experimental animal
Base grain carries out sterilization treatment.Then experimental animal base grain obtained is base grain after sterilizing, then subsequent nutriment addition and granulation
Require aseptically to carry out it is sterile to guarantee, but guarantee nutriment addition and pelletization aseptically just into
Row difficulty is higher.
Preferably, sterilization treatment can be after obtaining primary aseptic experiment mouse feed, to primary aseptic experiment mouse feed
Sterilization treatment is carried out, but at this time after sterilization treatment, subsequent pelletization needs aseptically to carry out, operated also the same tired
It is difficult.It is highly preferred that sterilization treatment is to carry out sterilization treatment after obtaining aseptic experiment mouse feed.It can be wrapped after sterilization treatment at this time
Dress.More convenient operation for entirety.
Further, sterilization treatment includes Co60 irradiation sterilization, absorbed dose 25-50kGy.
Further, sterilization treatment includes high-temperature sterilization, and sterilising temp is 121-131 DEG C, and the time is 15-30 minutes.
Preferably, for guarantee sterilizing completely, can two kinds of sterilization methods be applied in combination.
Nutriment is added into experimental animal base grain, primary aseptic experiment mouse feed is made, so that primary aseptic experiment mouse
B family vitamin content is higher than the 3-5% of B family vitamin content in aseptic experiment mouse feed in feed.
A kind of aseptic experiment mouse feed provided by the invention is specifically described below in conjunction with specific embodiment.
Embodiment 1
The embodiment of the invention provides a kind of aseptic experiment mouse feeds and preparation method thereof.
A kind of preparation method of aseptic experiment mouse feed, comprising:
40kg corn flour, 25kg wheat bran, 28kg dregs of beans, 0.1kg salt, 0.9kg bone meal and 0.9kg lysine is weighed to stir
It mixes and is uniformly mixed obtained experimental animal base grain.
Various nutritional ingredients in test experience animal base grain, measuring wherein vitamin A content is 23IU/g, vitamin D
Content is 0.04IU/g, content of vitamin E 6IU/g, Vitamin K3 content are 0.8mg/kg, vitamin B1 content is 10mg/
Kg, vitamin B2 content be 6mg/kg, vitamin B6 3mg/kg, niacin 62mg/kg, pantothenic acid 11mg/kg, folic acid 0.9mg/
Kg, biotin 0.23mg/kg, vitamin B12 0.04mg/kg, organic selenium 0.2mg/kg, organic zinc 15mg/kg.
The aseptic experiment mouse feed nutrition content that will be mentioned in the above-mentioned nutritional ingredient detected above and S2 step
Standard compares.The ingredient for being unsatisfactory for standard to content adds corresponding single nutriment, so that it meets above-mentioned standard,
Wherein B family vitamin additive amount, which is above standard, the 3% of content minimum is made primary aseptic experiment mouse feed.
By primary aseptic experiment mouse feed after granulator is pelletized, through 25kGy Co60 irradiation sterilization.Then again through 121
DEG C high-temperature sterilization 30 minutes, obtain finished product aseptic experiment mouse feed provided in this embodiment.
It is as follows to measure each nutrition content in obtained finished product aseptic experiment mouse feed: vitamin A content 28IU/g,
Vitamin D content is 1.5IU/g, content of vitamin E 120IU/g, Vitamin K3 content are 20mg/kg, vitamin B1 content
For 26mg/kg, vitamin B2 content be 24mg/kg, vitamin B6 20mg/kg, niacin 60mg/kg, pantothenic acid 24mg/kg, leaf
Sour 4mg/kg, biotin 0.2mg/kg, vitamin B12 0.03mg/kg, organic selenium 0.2mg/kg, organic zinc 60mg/kg.
Embodiment 2
The embodiment of the invention provides a kind of aseptic experiment mouse feeds and preparation method thereof.
A kind of preparation method of aseptic experiment mouse feed, comprising:
43kg corn flour, 10kg wheat bran, 30kg dregs of beans, 0.3kg salt, 1.1kg bone meal and 1.1kg lysine is weighed to stir
It mixes and is uniformly mixed obtained experimental animal base grain.
Various nutritional ingredients in test experience animal base grain, measuring wherein vitamin A content is 13IU/g, vitamin D
Content is 0.081U/g, content of vitamin E 6.3IU/g, Vitamin K3 content are 0.7mg/kg, vitamin B1 content is
11mg/kg, vitamin B2 content be 7mg/kg, vitamin B6 6mg/kg, niacin 63mg/kg, pantothenic acid 25mg/kg, folic acid
0.3mg/kg, biotin 0.28mg/kg, vitamin B12 0.05mg/kg, organic selenium 0.18mg/kg, organic zinc 7mg/kg.
The aseptic experiment mouse feed nutrition content that will be mentioned in the above-mentioned nutritional ingredient detected above and S2 step
Standard compares.The ingredient for being unsatisfactory for standard to content adds corresponding single nutriment, so that it meets above-mentioned standard,
Wherein B family vitamin additive amount, which is above standard, the 4% of content minimum is made primary aseptic experiment mouse feed.
By primary aseptic experiment mouse feed after granulator is pelletized, through 50kGy Co60 irradiation sterilization.Then again through 131
DEG C high-temperature sterilization 15 minutes, obtain finished product aseptic experiment mouse feed.
It is as follows to measure each nutrition content in obtained finished product aseptic experiment mouse feed: vitamin A content 14IU/g,
Vitamin D content is 4.3IU/g, content of vitamin E 240IU/g, Vitamin K3 content are 5mg/kg, vitamin B1 content is
13mg/kg, vitamin B2 content be 12mg/kg, vitamin B6 24mg/kg, niacin 120mg/kg, pantothenic acid 48mg/kg, folic acid
8mg/kg, biotin 0.4mg/kg, vitamin B12 0.06mg/kg, organic selenium 0.4mg/kg, organic zinc 120mg/kg.
Embodiment 3
The embodiment of the invention provides a kind of aseptic experiment mouse feeds and preparation method thereof.
A kind of preparation method of aseptic experiment mouse feed, comprising:
It is mixed to weigh 42kg corn flour, 27kg wheat bran, 29kg dregs of beans, 0.2kg salt, 1kg bone meal and the stirring of 1kg lysine
Close uniformly obtained experimental animal base grain.
Various nutritional ingredients in test experience animal base grain, measuring wherein vitamin A content is 29IU/g, vitamin D
Content is 0.141U/g, content of vitamin E 10.1IU/g, Vitamin K3 content are 0.9mg/kg, vitamin B1 content is
10mg/kg, vitamin B2 content be 10mg/kg, vitamin B6 9mg/kg, niacin 69mg/kg, pantothenic acid 19mg/kg, folic acid
0.8mg/kg, biotin 0.16mg/kg, vitamin B12 0.04mg/kg, organic selenium 0.2mg/kg, organic zinc 20mg/kg.
The aseptic experiment mouse feed nutrition content standard that will be mentioned in the above-mentioned nutritional ingredient detected and S2 step
It compares.The ingredient for being unsatisfactory for standard to content adds corresponding single nutriment, so that it meets above-mentioned standard.It estimates
Subsequent disinfecting process and pelletization make B family vitamin loss amount in experimental animal base grain be 3%.To experimental animal base grain
Middle extra-nutrition substance obtains primary aseptic experiment mouse feed, wherein the B family vitamin supplemented is by vitamin micro-capsule coating.B race
The magnitude of recruitment of vitamin is the experimental animal base that each component content and actual monitoring arrive in aseptic experiment mouse grain nutriment standard
The difference of the various nutritional ingredients of grain, and plus the loss amount of B family vitamin in experimental animal base grain.
By primary aseptic experiment mouse feed after granulator is pelletized, through 35kGy Co60 irradiation sterilization.Then again through 126
DEG C high-temperature sterilization 20 minutes, obtain finished product aseptic experiment mouse feed.
It is as follows to measure each nutrition content in obtained finished product aseptic experiment mouse feed: vitamin A content 22IU/g,
Vitamin D content is 2.3IU/g, content of vitamin E 150IU/g, Vitamin K3 content are 8mg/kg, vitamin B1 content is
19mg/kg, vitamin B2 content be 17mg/kg, vitamin B6 16mg/kg, niacin 80mg/kg, pantothenic acid 30mg/kg, folic acid
5mg/kg, biotin 0.3mg/kg, vitamin B12 0.04mg/kg, organic selenium 0.3mg/kg, organic zinc 80mg/kg.
Embodiment 4
The embodiment of the invention provides a kind of aseptic experiment mouse feeds and preparation method thereof.
A kind of preparation method of aseptic experiment mouse feed, comprising:
It is mixed to weigh 42kg corn flour, 15kg wheat bran, 29kg dregs of beans, 0.1kg salt, 1kg bone meal and the stirring of 1kg lysine
Close uniformly obtained experimental animal base grain.
Various nutritional ingredients in test experience animal base grain, measuring wherein vitamin A content is 17IU/g, vitamin D
Content is 0.112U/g, content of vitamin E 7.8IU/g, Vitamin K3 content are 0.8mg/kg, vitamin B1 content is
10mg/kg, vitamin B2 content be 9mg/kg, vitamin B6 8mg/kg, niacin 66mg/kg, pantothenic acid 21mg/kg, folic acid
0.7mg/kg, biotin 0.19mg/kg, vitamin B12 0.04mg/kg, organic selenium 0.1mg/kg, organic zinc 11mg/kg.
The aseptic experiment mouse feed nutrition content standard that will be mentioned in the above-mentioned nutritional ingredient detected and S2 step
It compares.The ingredient for being unsatisfactory for standard to content adds corresponding single nutriment, so that it meets above-mentioned standard.It estimates
Subsequent disinfecting process and pelletization make B family vitamin loss amount in experimental animal base grain be 5%.To experimental animal base grain
Middle extra-nutrition substance obtains primary aseptic experiment mouse feed, wherein the B family vitamin supplemented is by vitamin micro-capsule coating.B race
The magnitude of recruitment of vitamin is the experimental animal base that each component content and actual monitoring arrive in aseptic experiment mouse grain nutriment standard
The difference of the various nutritional ingredients of grain, and plus the loss amount of B family vitamin in experimental animal base grain.
By primary aseptic experiment mouse feed after granulator is pelletized, through 45kGy Co60 irradiation sterilization.Then again through 128
DEG C high-temperature sterilization 25 minutes, obtain finished product aseptic experiment mouse feed.
It is as follows to measure each nutrition content in obtained finished product aseptic experiment mouse feed: vitamin A content 26IU/g,
Vitamin D content is 3.4IU/g, content of vitamin E 190IU/g, Vitamin K3 content are 10mg/kg, vitamin B1 content
For 23mg/kg, vitamin B2 content be 20mg/kg, vitamin B6 19mg/kg, niacin 100mg/kg, pantothenic acid 42mg/kg, leaf
Sour 6mg/kg, biotin 0.3mg/kg, vitamin B12 0.05mg/kg, organic selenium 0.4mg/kg, organic zinc 100mg/kg.
Comparative example 1
This comparative example is substantially the same manner as Example 1, the difference is that adopting after directly experimental animal base grain is pelletized
Sterilizing experimental animal base grain is obtained with disinfection way same as Example 1.
Comparative example 2
This comparative example is substantially the same manner as Example 1, the difference is that, the B family vitamin of addition is replaced into equivalent
Glucose.
Comparative example 3
This comparative example is substantially the same manner as Example 1, the difference is that, the Vitamin K3 of addition is replaced into the Portugal of equivalent
Grape sugar.
Comparative example 4
This comparative example is substantially the same manner as Example 1, the difference is that, the B family vitamin of addition is replaced into equivalent
Vitamin K3.
Comparative example 5
This comparative example is substantially the same manner as Example 1, the difference is that, the Vitamin K3 of addition is replaced into the B of equivalent
Family vitamin, replacement amount is by vitamin B1: vitamin B2: vitamin B6=1:1:5 is allocated.
Experimental example
The aseptic experiment mouse feed that embodiment 1-4 is provided and the sterilizing experimental animal base grain that comparative example provides are differentiated and numbered
1-9.Choose the identical aseptic experiment mouse of 14 physiological status.Be equally divided into 9 groups, by 5 groups of mouse feed respectively No. 1-9 it is sterile
Experimental mouse grain 30d.Again the 31st day when, by every group of mouse knife blade, quickly cut tail portion at Mouse Tail-tip 3mm, immediately
With manual time-keeping, a blood is inhaled at tail point every 5s filter paper, when stopping timing, i.e. tail bleeding when on filter paper without blutpunkte
Between.Each group group bleeding time average value is recorded to table 1.
1 each group mouse tail bleeding average time of table counts (s)
From table 1, it can be seen that using aseptic experiment mouse feed provided in an embodiment of the present invention, aseptic experiment mouse is fed,
Mouse be wound after clotting time in 35s or so.And after being wound using the mouse that experimental animal base grain after common sterilizing is fed
Average clotting time be 62.5s, the clotting time for feeding the mouse of aseptic experiment mouse feed provided by the invention is significantly shorter than
The clotting time of the common sterilizing experimental animal base grain of feeding.It can be seen that being fed using aseptic experiment mouse feed provided by the invention
Aseptic experiment mouse is supported, germfree animal Wound healing can be promoted.
And comparative example 2 is few relative to embodiment 1B race constituent content, be unsatisfactory for the present invention claims B race constituent content, the 6th
Group clotting time of mice it is long compared with the 1st group of clotting time, it can be seen that, B race element the present invention claims in the range of when, help
In aseptic experiment mouse blood coagulation.Comparative example 3 is low relative to 1 Vitamin K3 content of experimental example, be unsatisfactory for the present invention claims dimension it is raw
Plain K3 content, and the 7th group of clotting time of mice is much longer compared with the 1st group of clotting time, and than the 6th group clotting time is long, thus may be used
See, Vitamin K3 the present invention claims in the range of when it is bigger to the facilitation of germfree animal Wound healing.
Embodiment 1, comparative example 2 and comparative example 4 are compared, comparative example 2 is relative to embodiment 1B race constituent content
Few, comparative example 4 replaces with vitamin relative to comparative example 2, by the amount of the difference of B race constituent content between comparative example 2 and embodiment 1
K3 is supplemented to comparative example 4, and therefore, comparative example 4 is higher relative to 2 Vitamin K3 content of comparative example.And comparative example 4 the corresponding 8th
The average clotting time of group mouse is only above the average clotting time 2.5s of the corresponding 6th group of mouse of comparative example 2, it can be seen that,
Increase Vitamin K3 usage amount make its usage amount exceed the present invention claims range, almost without the work for increasing coagulating effectiveness again
With.
Embodiment 1, comparative example 3 and comparative example 5 are compared, comparative example 3 is relative to 1 Vitamin K3 content of embodiment
Few, comparative example 5 replaces with B race dimension relative to comparative example 3, by the amount of the difference of B race constituent content between comparative example 3 and embodiment 1
Raw element, therefore, comparative example 5 is higher relative to comparative example 3B family vitamin content.And the corresponding 8th group of mouse of comparative example 4 is flat
Equal clotting time the 6th group of average clotting time of mouse corresponding with comparative example 2 is identical, it can be seen that, increase making for vitamin B
Dosage make its usage amount exceed the present invention claims range, increase the effect of coagulating effectiveness again.
And the 8th group and the 9th group is compared with embodiment 1, it will be apparent that 1 clotting time of embodiment is significantly shorter than the 8th group
With the 9th group.It can be seen that vitamin A and B family vitamin, which are used cooperatively, to be had the function of cooperateing with blood coagulation.
In conclusion aseptic experiment mouse feed provided by the invention, contains abundant and comprehensive various aseptic experiment mouse
Required vitamin enables to aseptic experiment mouse to grow up healthy and sound, B family vitamin especially rich in and vitamin K, energy
Germfree mouse is enough avoided to be free of the normal physiological activity that microorganism can not synthesize vitamin and influence experiment mice, energy due to itself
The resistance for enough improving mouse, especially can also effectively avoid germfree mouse wound after testing from being not easy the phenomenon that healing.
Rationally, nothing provided by the invention can be made in aseptic experiment mouse feed provided by the invention, production process setting
Bacterium tests mouse feed.
The foregoing is merely the preferred embodiment of the present invention, are not intended to restrict the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of aseptic experiment mouse feed, which is characterized in that the aseptic experiment mouse feed includes:
14-28IU/g vitamin A, 1.5-4.3IU/g vitamin D, 120-240IU/g vitamin E, 5-20mg/kg Vitamin K3,
13-26mg/kg vitamin B1,12-24mg/kg vitamin B2,12-24mg/kg vitamin B6,60-120mg/kg niacin, 24-
48mg/kg pantothenic acid, 4-8mg/kg folic acid, 0.2-0.4mg/kg biotin, 0.03-0.06mg/kg vitamin B12.
2. aseptic experiment mouse feed according to claim 1, which is characterized in that the aseptic experiment mouse feed further include:
0.2-0.4mg/kg organic selenium.
3. aseptic experiment mouse feed according to claim 1, which is characterized in that the aseptic experiment mouse feed further include:
60-120mg/kg organic zinc.
4. a kind of preparation method of aseptic experiment mouse feed characterized by comprising
Each nutrient composition content in experimental animal base grain made from detection in advance；
Using following nutrition content as aseptic experiment mouse feed nutrition content standard:
14-28IU/g vitamin A, 1.5-4.3IU/g vitamin D, 120-240IU/g vitamin E, 5-20mg/kg Vitamin K3,
13-26mg/kg vitamin B1,12-24mg/kg vitamin B2,12-24mg/kg vitamin B6,60-120mg/kg niacin, 24-
48mg/kg pantothenic acid, 4-8mg/kg folic acid, 0.2-0.4mg/kg biotin, 0.03-0.06mg/kg vitamin B12；
By the aseptic experiment mouse feed nutrition content standard, nutriment is added into experimental animal base grain, primary is made
Aseptic experiment mouse feed；
Aseptic experiment mouse feed as described in claim 1 is made in the primary aseptic experiment mouse feed after pelletizing.
5. the preparation method of aseptic experiment mouse feed according to claim 4, which is characterized in that the experimental animal base
The nutriment added in grain is added again after the B group family vitamin in the nutriment is used vitamin micro-capsule coating
To in the experimental animal base grain.
6. the preparation method of aseptic experiment mouse feed according to claim 4, which is characterized in that compare the aseptic experiment mouse
Feed nutrition substance content standard adds nutriment into experimental animal base grain and primary aseptic experiment mouse feed is made, so that
B family vitamin content is higher than B family vitamin content in the aseptic experiment mouse feed in the primary aseptic experiment mouse feed
3-5%.
7. the preparation method of aseptic experiment mouse feed according to claim 4, which is characterized in that in test experience animal base
Sterilization treatment is carried out to experimental animal base grain before each nutrient composition content in grain.
8. according to the preparation method of aseptic experiment mouse feed as claimed in claim 4, which is characterized in that obtain the sterile reality of primary
It tests after mouse feed, sterilization treatment is carried out to the primary aseptic experiment mouse feed or the aseptic experiment mouse feed.
9. the preparation method of aseptic experiment mouse feed according to claim 7 or 8, which is characterized in that the sterilization treatment
Including Co60 irradiation sterilization, absorbed dose 25-50kGy.
10. the preparation method of aseptic experiment mouse feed according to claim 7 or 8, which is characterized in that the sterilization treatment
Including high-temperature sterilization, sterilising temp is 121-131 DEG C, and the time is 15-30 minutes.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111685238A (en) * | 2020-07-15 | 2020-09-22 | 常州鼠一鼠二生物科技有限公司 | Sterile feed for large sterile mice and preparation method thereof |
| CN112602852A (en) * | 2020-12-04 | 2021-04-06 | 苏州双狮实验动物饲料科技有限公司 | Irradiation mouse feed and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2410964A1 (en) * | 1977-12-07 | 1979-07-06 | Sanders | Rodent food and rodenticides in sausage form - contg. a high proportion of water |
| CN1742609A (en) * | 2005-10-11 | 2006-03-08 | 中国医学科学院实验动物研究所 | The breeding feed formulation of sterilized rat |
| CN1748493A (en) * | 2005-10-11 | 2006-03-22 | 中国医学科学院实验动物研究所 | Cultivation technology and the feed formula of aseptic cavy |
-
2018
- 2018-09-27 CN CN201811133962.9A patent/CN109123102A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2410964A1 (en) * | 1977-12-07 | 1979-07-06 | Sanders | Rodent food and rodenticides in sausage form - contg. a high proportion of water |
| CN1742609A (en) * | 2005-10-11 | 2006-03-08 | 中国医学科学院实验动物研究所 | The breeding feed formulation of sterilized rat |
| CN1748493A (en) * | 2005-10-11 | 2006-03-22 | 中国医学科学院实验动物研究所 | Cultivation technology and the feed formula of aseptic cavy |
Non-Patent Citations (2)
| Title |
|---|
| 宋盛宪等: "《日本对虾健康养殖》", 31 March 2004, 海洋出版社 * |
| 李文霞: "无菌小鼠营养配方的初步研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111685238A (en) * | 2020-07-15 | 2020-09-22 | 常州鼠一鼠二生物科技有限公司 | Sterile feed for large sterile mice and preparation method thereof |
| CN112602852A (en) * | 2020-12-04 | 2021-04-06 | 苏州双狮实验动物饲料科技有限公司 | Irradiation mouse feed and preparation method thereof |
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