CN1727362B - 癌胚抗原阳性肿瘤治疗性疫苗的制备及应用 - Google Patents
癌胚抗原阳性肿瘤治疗性疫苗的制备及应用 Download PDFInfo
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- CN1727362B CN1727362B CN200410053313XA CN200410053313A CN1727362B CN 1727362 B CN1727362 B CN 1727362B CN 200410053313X A CN200410053313X A CN 200410053313XA CN 200410053313 A CN200410053313 A CN 200410053313A CN 1727362 B CN1727362 B CN 1727362B
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CN200410053313XA CN1727362B (zh) | 2004-07-30 | 2004-07-30 | 癌胚抗原阳性肿瘤治疗性疫苗的制备及应用 |
PCT/CN2005/000763 WO2006010308A1 (fr) | 2004-07-30 | 2005-05-31 | Preparation et application de vaccins therapeutiques a une tumeur |
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CN200410053313XA CN1727362B (zh) | 2004-07-30 | 2004-07-30 | 癌胚抗原阳性肿瘤治疗性疫苗的制备及应用 |
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CN1865281B (zh) | 2006-06-02 | 2012-08-22 | 北京大学人民医院 | 卵巢癌抗独特型抗体6b11 t细胞表位肽及其应用 |
CN100391980C (zh) * | 2006-08-02 | 2008-06-04 | 中国人民解放军军事医学科学院基础医学研究所 | 抗肿瘤多靶点复合抗原及其编码基因与应用 |
BRPI0814140A2 (pt) * | 2007-07-27 | 2017-03-28 | Immatics Biotechnologies Gmbh | composição de peptídeos associados a tumor e vacina anticâncer relacionada |
CN102154223A (zh) * | 2010-02-11 | 2011-08-17 | 中国人民解放军第二军医大学 | 携带融合蛋白基因的重组腺病毒及其制备方法和应用 |
CN105693862B (zh) * | 2016-03-07 | 2019-06-18 | 中国人民解放军第二军医大学 | 抗融合蛋白cea576-669hsp70l1单克隆抗体4e8及其用途 |
CN105693863B (zh) * | 2016-03-07 | 2019-06-18 | 中国人民解放军第二军医大学 | 抗融合蛋白cea576-669hsp70l1单克隆抗体3c1及其用途 |
CN106749673B (zh) * | 2016-11-22 | 2021-07-02 | 张震宇 | 一种融合蛋白、制备方法及其应用 |
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CN1270635A (zh) * | 1997-08-05 | 2000-10-18 | 斯特思吉生物技术公司 | 包含hpv抗原和应激蛋白或者其表达载体的组合物激发的抗hpv抗原免疫应答 |
CN1491116A (zh) * | 2001-02-05 | 2004-04-21 | \ | Hbv治疗方法 |
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DK0700445T3 (da) * | 1993-06-04 | 2002-05-13 | Whitehead Biomedical Inst | Stressproteiner og anvendelser deraf |
CN1411512A (zh) * | 1999-10-20 | 2003-04-16 | 约翰霍普金斯大学医学院 | 嵌合的免疫原性组合物及其编码核酸 |
CN1141142C (zh) * | 2001-02-08 | 2004-03-10 | 北京迪威华宇生物技术有限公司 | 人乳腺癌muc-1抗原基因工程疫苗 |
CN1517437B (zh) * | 2003-01-15 | 2010-06-09 | 张聪慧 | 特异性治疗肿瘤或胞内感染的疫苗及应用 |
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CN1270635A (zh) * | 1997-08-05 | 2000-10-18 | 斯特思吉生物技术公司 | 包含hpv抗原和应激蛋白或者其表达载体的组合物激发的抗hpv抗原免疫应答 |
CN1491116A (zh) * | 2001-02-05 | 2004-04-21 | \ | Hbv治疗方法 |
Non-Patent Citations (4)
Title |
---|
马俊芬.转染癌胚抗原基因的人树突状细胞疫苗表达的研究.河南肿瘤学杂志16 3.2003,16(3),164-166. |
马俊芬.转染癌胚抗原基因的人树突状细胞疫苗表达的研究.河南肿瘤学杂志16 3.2003,16(3),164-166. * |
黄爱强.带免疫佐剂CEA基因疫苗的构建一起稳定同步表达的研究.癌症23 3.2003,23(3),282-287. |
黄爱强.带免疫佐剂CEA基因疫苗的构建一起稳定同步表达的研究.癌症23 3.2003,23(3),282-287. * |
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