CN1669413A - Marine finishes life preserving method without water - Google Patents
Marine finishes life preserving method without water Download PDFInfo
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- CN1669413A CN1669413A CN 200410006264 CN200410006264A CN1669413A CN 1669413 A CN1669413 A CN 1669413A CN 200410006264 CN200410006264 CN 200410006264 CN 200410006264 A CN200410006264 A CN 200410006264A CN 1669413 A CN1669413 A CN 1669413A
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Abstract
A waterless activation method of the see fishes. Keep the sea fishes in the pure oxygen condition on the waterless state by domesticating without food and episodic gradiently cooling process, the sea fishes can be alive for a long time and reactivated in the fresh seawater. The invention can make some sea fishes be alive for about 60 hours, and the weight of the fish is only lost of 2.4 percent, so the active effect is good, it improves the fresh activity and accumulating of the sea fishes oberviously, and has the great acceleration significance to the development of mariculture.
Description
Technical field
The present invention relates to the keepalive method of a kind of marine fishes, specifically relate to the method for keep-alive and the transportation under cryogenic conditions of marine fishes under a kind of anhydrous situation.
Background technology
Along with the continuous development of aquaculture industry, also more and more higher to the requirement of the keep-alive techniques in the aquatic products transportation.Fishery developed countries such as Japan are applied to the ecological ice-temperature technology length of part aquatic/marine animals in succession apart from the waterless keep-alive transportation research.China also begins to have carried out in this respect some researchs, just reports tests such as employing low temperature keep-alive techniques preservation Ruditapes philippinarum and japonicus on " Shandong fishery " magazine in succession, and survival rate is higher.
And for marine fishes, it is blank that research in this respect also belongs to.At present at most of marine fishes, lefteye flounder etc. for example, the keepalive method that is adopted in transportation be still some traditional band water transport transmission methods basically.Be about to fish and put into seawater and charge into the live fish carrier method of pure oxygen continuously and fish is put into seawater and charge into pure oxygen packages sealed case conveying method, but above method all exists the keep-alive time to fall short of, effectively seawater easily causes and pollutes and problem such as corrosion in low, the transportation of carrying capacity.
Summary of the invention
The objective of the invention is defective, provide a kind of and can be prolonged the marine fishes time-to-live greatly so that the weight of long-distance transportation and fish reduces small keepalive method at existing marine fishes keep-alive techniques.
The theory of institute of the present invention foundation is the ecological ice-temperature theory: promptly according to the natural law of animal hibernation, breathing environment and the environmental temperature of marine fishes all is controlled in the temperature range of similar hibernation, again it returned to original state after after a while.
The waterless keep-alive method of marine fishes of the present invention may further comprise the steps:
(1) raises and train: selected fish are put into seawater, stop feeding, simultaneously seawater is lowered the temperature;
(2) anhydrous oxygenation: after raising and train end, fish is packed in the air bag, charge into pure oxygen;
(3) low-temperature preservation: packaged fish is moved into incubator, temperature control low-temperature preservation;
(4) recover: will take out through the fish after the preservation, and put into seawater and slowly return to original state.
Wherein, when the seawater of raising and train fish is lowered the temperature, adopt the method for gradient cooling.
When selecting flounder fish to carry out waterless keep-alive, should should be controlled between-0.5 ℃ to 20 ℃ the temperature range of carrying out the temperature control preservation after its oxygenation more than 48 hours the time of raising and train before the flounder fish keep-alive.
Owing to adopt waterless keep-alive method of the present invention to preserve marine fishes, the keep-alive time of marine fishes is prolonged greatly, and the weight of fish have only slightly and alleviate.With the flounder fish is example, adopt waterless keep-alive method of the present invention after, the keep-alive time can reach about 60 hours, it only is 2.4% of its body weight that the weight of fish reduces.And this technology is applied to can obtain effect preferably too under the situation of other marine fishes such as star eel, turbot.
The waterless keep-alive method of marine fishes of the present invention has been improved the fresh and alive accumulating of marine fishes significantly, can greatly promote the development of mariculture industry.
Embodiment
Describe marine fishes waterless keep-alive method of the present invention with flounder fish in detail as one of concrete embodiment below.
(1) select: will have the fish of scar, vigor difference to choose, it is better to choose physique, health, great-hearted flounder fish.
(2) raise and train: the flounder fish selected is raised and train in seawater more than 48 hours under the situation of feeding stopping, making the food in its digestive tract digest discharge substantially, lower its oxygen demand and stress reaction.When raising and train, with the mode of gradient cooling progressively begin to reduce water temperature, at rate of temperature fall more than 10 ℃ for per hour smaller or equal to 2 ℃; Below 10 ℃, rate of temperature fall is for per hour smaller or equal to 0.5 ℃.Allow flounder fish adaptation low temperature environment progressively, so that the low temperature keep-alive in later stage.
(3) anhydrous oxygenation: after raising and train end flounder fish is taken out from seawater, move in the air bag of anhydrous bilayer, and in bag, charge into sufficient pure oxygen, to guarantee the survival of flounder fish in the transportation preserving process.
(4) low-temperature preservation: air bag is moved in the incubator, the incubator temperature is controlled between-0.5 ℃ to 20 ℃.In preserving process, reduce vibrations, avoid the damage of fish body.
(5) recover: after low-temperature preservation finishes, pure and fresh seawater is put in the flounder fish taking-up made it slowly return to original state.
Below in conjunction with data present embodiment is further specified:
The comparison sheet of table one lefteye flounder waterless keep-alive time when taking different rate of temperature fall under the gradient cooling.
Table two is for raising and train the influence comparison sheet of time to the lefteye flounder waterless keep-alive time.
Table three is the breathing frequency comparison sheet of lefteye flounder under the different temperatures.
Table four is lefteye flounder waterless keep-alive time and a body weight change comparison sheet under the different temperatures.
Table one
| The cooling gradient | Rate of temperature fall/℃ h -1 | Waterless keep-alive time/h |
| More than 10 ℃ below 10 ℃ | ????≤2 ????≤0.5 | ????63 |
| More than 10 ℃ below 10 ℃ | ????≤3 ????≤0.5 | ????54 |
| More than 10 ℃ below 10 ℃ | ????≤4 ????≤1 | ????35 |
As above shown in the table, when improving the rate of temperature fall of lefteye flounder in seawater, the waterless keep-alive time of lefteye flounder shortens, reason is that fish are when its environment water temperature changes, to produce stress reaction, when variation of ambient temperature is slow, the stress reaction of fish a little less than, can adapt to its variation and not influence its normal survival; And when variation of ambient temperature is very fast, fish will produce strong stress reaction.Though can not cause death, can cause the delay death of fish, reduce the waterless keep-alive time.
Table two
| Raise and train the time (h) | ????0 | ????24 | ????48 | ????72 | ????96 |
| The keep-alive time (h) | ????32 | ????51 | ????64 | ????63 | ????64 |
As above shown in the table, lefteye flounder is lowered the temperature and waterless keep-alive immediately as not raising and train, 32 hours time-to-live, and respectively through raising and train 24,48,72,96, hour after, its corresponding waterless keep-alive time is 51,64,63,64 hours.As seen raise and train the waterless keep-alive time that obviously to improve lefteye flounder in 48 hours, again time expand, then do not have positive effect.This is because the fish of state of being satiated with food need consume more oxygen, and to the stress reaction sensitivity, and after raising and train through stopping eating, the food in its digestive tract has been arranged substantially, and oxygen demand descends, and stress action degree reduces, and has obviously improved the keep-alive time.
Table three
| Temperature/℃ | Breathe frequency/time min -1 | Remarks |
| ?18.2 ?15.0 ?11.0 ?9.0 ?7.0 ?5.0 ?4.0 ?3.0 ?2.0 ?1.5 ?0.0 ?-0.3 ?-0.5 ?-0.7 ?-1.0 ?-1.2 | ????25——26 ????25——26 ????24——26 ????20——23 ????20——23 ????18——20 ????12——15 ????12——13 ????11——12 ????10——11 ????4——8 ????2——6 ????2——6 ????2——6 ????0.2——3 ????0.2——3 | It is all dead to breathe the extremely irregular survival in short-term of the irregular breathing of beginning back |
As above shown in the table, along with the continuous decline of temperature, the breathing frequency of lefteye flounder is also in continuous reduction.Breathe frequency and also begin irregularly in the time of 0 ℃, during to-1.0 ℃, it is more obvious to breathe the erratic situation of frequency, and when arriving-1.2 ℃, the lefteye flounder back of surviving in short-term is all dead.Thereby the existence critical temperature that can learn lefteye flounder is basically in-0.7 ℃ of-0 ℃ of scope.To remain on about-0.5 ℃ to well.
Table four
| Temperature/℃ | ????13-20 | ????7-10 | ????3-5 | ????-0.5-1.0 |
| Keep-alive time/h | ????27 | ????42 | ????53 | ????68 |
| Body weight change/% | ????-2.8 | ????-4.2 | ????-3.8 | ????-2.4 |
As above shown in the table, in the temperature range that lefteye flounder can be survived, temperature is low more, and the consumption of fish body is few more, thereby makes the keep-alive time of fish elongated, and is minimum with macrura reevesii body weightlessness.
Claims (4)
1, a kind of marine fishes waterless keep-alive method is characterized in that may further comprise the steps:
(1) raises and train: selected fish are put into seawater, stop feeding, simultaneously seawater is lowered the temperature;
(2) anhydrous oxygenation: after raising and train end, fish is packed in the air bag, charge into pure oxygen;
(3) low-temperature preservation: packaged fish is moved into incubator, temperature control low-temperature preservation;
(4) recover: will take out through the fish after the preservation, and put into seawater and slowly return to original state.
2, a kind of marine fishes waterless keep-alive method as claimed in claim 1 is characterized in that: when the seawater of raising and train fish is lowered the temperature, adopt the method for gradient cooling.
3, a kind of marine fishes waterless keep-alive method as claimed in claim 1 is characterized in that: when selecting flounder fish to carry out waterless keep-alive, to the time of raising and train before the flounder fish keep-alive should be more than 48 hours.
4, a kind of marine fishes waterless keep-alive method as claimed in claim 1 is characterized in that: when selecting flounder fish to carry out waterless keep-alive, should be controlled between-0.5 ℃ to 20 ℃ the temperature range of carrying out the temperature control preservation after the anhydrous oxygenation of flounder fish.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410006264 CN1669413A (en) | 2004-03-19 | 2004-03-19 | Marine finishes life preserving method without water |
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| CN 200410006264 CN1669413A (en) | 2004-03-19 | 2004-03-19 | Marine finishes life preserving method without water |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940174A (en) * | 2010-07-30 | 2011-01-12 | 淮阴工学院 | Method for anhydrously surviving freshwater fish through repeated dormancy |
| CN101138331B (en) * | 2007-10-31 | 2011-04-13 | 贵州省水产研究所 | Alive sturgeon low-temperature anhydrous transportation technology |
| CN102599098A (en) * | 2011-12-16 | 2012-07-25 | 中国水产科学研究院东海水产研究所 | Eleutheronema rhadinum fry live transport method |
| CN102823523A (en) * | 2011-09-06 | 2012-12-19 | 山东商业职业技术学院 | Non-water conservation method of aquatic product and industrialization implement system |
| CN104920251A (en) * | 2014-12-26 | 2015-09-23 | 浙江省海洋水产研究所 | Paralichthys olivaceus transportation method capable of keeping paralichthys olivaceus alive without water |
| CN107853236A (en) * | 2017-11-13 | 2018-03-30 | 广东海洋大学 | A kind of anhydrous transportation method of seawater grouper |
| CN108477031A (en) * | 2018-03-20 | 2018-09-04 | 广东海洋大学 | A kind of anhydrous transportation method using cold carbon dioxide narcosis seawater grouper |
| CN108719147A (en) * | 2018-05-25 | 2018-11-02 | 广东海洋大学 | A kind of long-distance transport method of seawater grouper |
| CN111655584A (en) * | 2017-12-27 | 2020-09-11 | 德飞时有限公司 | Artificial hibernation induction method for fish, live fish packaging method, and live fish packaging container |
| CN111937786A (en) * | 2020-08-26 | 2020-11-17 | 上海海洋大学 | Ecological ice-temperature water-free keep-alive method for lateolabrax japonicus |
-
2004
- 2004-03-19 CN CN 200410006264 patent/CN1669413A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138331B (en) * | 2007-10-31 | 2011-04-13 | 贵州省水产研究所 | Alive sturgeon low-temperature anhydrous transportation technology |
| CN101940174A (en) * | 2010-07-30 | 2011-01-12 | 淮阴工学院 | Method for anhydrously surviving freshwater fish through repeated dormancy |
| CN102823523A (en) * | 2011-09-06 | 2012-12-19 | 山东商业职业技术学院 | Non-water conservation method of aquatic product and industrialization implement system |
| CN102823523B (en) * | 2011-09-06 | 2014-04-30 | 山东商业职业技术学院 | Non-water conservation method of aquatic product |
| CN102599098A (en) * | 2011-12-16 | 2012-07-25 | 中国水产科学研究院东海水产研究所 | Eleutheronema rhadinum fry live transport method |
| CN104920251A (en) * | 2014-12-26 | 2015-09-23 | 浙江省海洋水产研究所 | Paralichthys olivaceus transportation method capable of keeping paralichthys olivaceus alive without water |
| CN107853236A (en) * | 2017-11-13 | 2018-03-30 | 广东海洋大学 | A kind of anhydrous transportation method of seawater grouper |
| CN111655584A (en) * | 2017-12-27 | 2020-09-11 | 德飞时有限公司 | Artificial hibernation induction method for fish, live fish packaging method, and live fish packaging container |
| US11330805B2 (en) | 2017-12-27 | 2022-05-17 | The Fish Co., Ltd. | Method for inducing artificial hibernation of fish, live fish packaging method, and live fish packaging container |
| CN111655584B (en) * | 2017-12-27 | 2022-09-27 | 德飞时有限公司 | Artificial hibernation induction method for fish, live fish packaging method, and live fish packaging container |
| CN108477031A (en) * | 2018-03-20 | 2018-09-04 | 广东海洋大学 | A kind of anhydrous transportation method using cold carbon dioxide narcosis seawater grouper |
| CN108719147A (en) * | 2018-05-25 | 2018-11-02 | 广东海洋大学 | A kind of long-distance transport method of seawater grouper |
| CN111937786A (en) * | 2020-08-26 | 2020-11-17 | 上海海洋大学 | Ecological ice-temperature water-free keep-alive method for lateolabrax japonicus |
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