CN102613117A - Method for increasing ice-temperature anhydrous transportation survival rate of turbots - Google Patents
Method for increasing ice-temperature anhydrous transportation survival rate of turbots Download PDFInfo
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- CN102613117A CN102613117A CN2012101023088A CN201210102308A CN102613117A CN 102613117 A CN102613117 A CN 102613117A CN 2012101023088 A CN2012101023088 A CN 2012101023088A CN 201210102308 A CN201210102308 A CN 201210102308A CN 102613117 A CN102613117 A CN 102613117A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a method for increasing ice-temperature anhydrous transportation survival rate of turbots. The method comprises the following steps of: stopping feeding the turbot prepared to be transported for two days, slowly decreasing the temperature of cultivation seawater to 5 DEG C, then, adding vitamin C into the cultivation seawater to 50 mg/L, adding trehalose to 0.1 g/L, continuously and slowly decreasing the temperature of the cultivation seawater to minus 0.5 DEG C, and continuously filling gas in the whole temperature decreasing process; and beginning to carry out anhydrous oxygenation packaging 10 minutes later, simultaneously, controlling the temperature to be between minus 0.5 DEG C to 2 DEG C. The method disclosed by the invention is applied to the field of transportation circulation of the turbots; and low-temperature tolerance and anti-stress capability of the turbots processed through the method disclosed by the invention are obviously increased, so that the ice-temperature anhydrous transportation survival rate of the turbots is increased.
Description
Technical field:
The present invention relates to the aquatic products transportation field of circulation, relate to a kind of method that turbot is iced warm anhydrous transportation survival rate that improves particularly.
Background technology:
Along with the increase to the fresh and living aquatic products demand of the development of culture fishery and market, aquatic product keep-alive transportation technology demand is also increasingly high.The keepalive method that present turbot is adopted in transportation still is some traditional band water transport transmission methods basically.Be about to fish and put into the live fish carrier method of seawater continuous charge and fish is put into seawater and charge into pure oxygen packages sealed case conveying method, but above method all exists the keep-alive time to fall short of, effectively seawater is prone to cause and pollutes and problem such as corrosion in low, the transportation of carrying capacity.Fishery developed countries such as Japan transport the length that the ecological ice-temperature technology is applied to the part aquatic/marine animals in succession apart from waterless keep-alive.China also begins to have carried out in this respect some researchs, reports tests such as employing low temperature keep-alive techniques preservation Ruditapes philippinarum, lefteye flounder, turbot, japonicus in succession.
The principle that fish are iced warm waterless keep-alive transportation is that the temperature of live fish is reduced near its ecological ice-temperature, makes fish be in dormancy or semidormancy state, reduces the metabolism of fish significantly, thereby realizes the warm anhydrous transport of living fish of ice.The warm keep-alive of fish employing ice needs earlier the temperature of live fish is reduced near its ecological ice-temperature when transporting, but causes stress reaction and the frostbite of fish easily, causes survival rate decline in the transportation.Slow gradient cooling is adopted in the anhydrous transportation experiment of the ice temperature of turbot at present both at home and abroad, charges into to be sealed in behind the pure oxygen to carry out keep-alive under the low temperature and transport; There is problem such as fish body frostbite and stress reaction in the transportation in this method, and the problem in the transportation of particularly long-time ice temperature is more outstanding.Therefore need take measures to improve tolerance, the reduction stress reaction of fish body to low temperature.
Summary of the invention:
The technical problem that the present invention will solve provides a kind of method that turbot is iced warm anhydrous transportation survival rate that improves, and increases tolerance, the reduction stress reaction of turbot to low temperature, ices the survival rate of warm anhydrous transportation to improve turbot.
The present invention realizes through following technical scheme:
A kind ofly improve the method that turbot is iced warm anhydrous transportation survival rate; The turbot of preparing transportation was stopped eating 2 days; Slowly reduce breeding seawater temperature to 5 ℃; In this breeding seawater, add vitamin C then to 50mg/L; Trehalose is to 0.1g/L; Continue slowly to reduce by 0.5 ℃ of breeding seawater temperature to, continuous charge in whole temperature-fall period; The anhydrous oxygenation packing of beginning is controlled temperature simultaneously at-0.5 ℃ to 2 ℃ behind the 10min.
Further, the cooling extent of described slow reduction breeding seawater temperature to 5 ℃ is that every 1h is not more than 2 ℃; Described slow reduction breeding seawater temperature to-0.5 ℃ cooling extent is that every 1h is not more than 1 ℃.
The present invention's beneficial effect compared with prior art:
1) trehalose is the nonreducing sugar that is made up of two glucose molecules, and self property is highly stable.Trehalose has magical protective effect to organism; Be because trehalose can form the diaphragm of uniqueness at cell surface under severe environmental conditions such as high temperature, high and cold, hyperosmosis and dry dehydration; Protected protein matter molecule consistency inactivation effectively, thereby the life process of the body that earns a bare living and biological characteristic.
2) vitamin C has another name called ascorbic acid, is a kind of water soluble vitamin.Participate in multiple reaction in vivo,, in biological oxidation and reduction and cell respiration, play an important role as participating in redox process.Ascorbic major physiological function is: promote the biosynthesis of ossein, be beneficial to the healing of tissue injury; The metabolism of tyrosine and tryptophan prolongs organism life-span in the promotion amino acid; Improve the utilization of iron, calcium and folic acid; Prevent the injury of radical pair body; Enhancing body is the anti-stress ability and the immunity of environment to external world.
3) turbot after handling through the present invention obviously improves the tolerance and the anti-stress ability of low temperature, thereby improved the survival rate that turbot is iced warm anhydrous transportation.
Embodiment:
Below in conjunction with specific embodiment technical scheme of the present invention is done further to explain.
In November, 2011; The 50 tail turbot (average weight 600g) that transportation will be prepared by general aquaculture company in the Qingdao City were stopped eating 2 days, put into the volume that 1 cubic metre of turbot breeding seawater is housed and be 2 cubic metres Glass fibre reinforced plastic tub, 12 ℃ of ocean temperatures; Adopt outdoor natural cooling method water temperature slowly to be reduced to 5 ℃ through 4h; In water body, add 50ug vitamin C, 0.1g trehalose then, adopt cooler to continue to be cooled to-0.5 ℃, continuous charge in whole temperature-fall period through 6h; The anhydrous oxygenation packing of beginning is controlled temperature simultaneously at-0.5 ℃ to 2 ℃ behind the 10min.
Simultaneously there are 50 tail turbot (average weight 600g) to stop eating equally in addition 2 days; Put into the volume that 1 cubic metre of turbot breeding seawater is housed and be 2 cubic metres Glass fibre reinforced plastic tub; 12 ℃ of ocean temperatures; Adopt outdoor natural cooling method water temperature slowly to be reduced to 5 ℃, adopt cooler to continue to be cooled to-0.5 ℃, continuous charge in the temperature-fall period then through 6h through 4h; The anhydrous oxygenation packing of beginning is controlled temperature simultaneously at-0.5 ℃ to 2 ℃ behind the 10min.
Finish to pick up counting from anhydrous oxygenation packing, behind the 50h, the seawater of turbot being put into 2 ℃ recovers, seawater continuous charge in the recovery process, and environmental temperature is 6 ℃.Recover to observe behind the 20h and calculate the turbot survival rate, the result shows that adopting the inventive method processed group survival rate is 98%, and the control group turbot survival rate of not adding vitamin C and trehalose is 88%.Explain that the inventive method can obviously improve the survival rate of the anhydrous transportation of ice temperature of turbot.
Claims (2)
1. one kind is improved the method that turbot is iced warm anhydrous transportation survival rate; It is characterized in that may further comprise the steps: the turbot that will prepare transportation was stopped eating 2 days; Slowly reduce breeding seawater temperature to 5 ℃; In this breeding seawater, add vitamin C then to 50mg/L; Trehalose is to 0.1g/L; Continue slowly to reduce by 0.5 ℃ of breeding seawater temperature to, continuous charge in whole temperature-fall period; The anhydrous oxygenation packing of beginning is controlled temperature simultaneously at-0.5 ℃ to 2 ℃ behind the 10min.
2. a kind of method that turbot is iced warm anhydrous transportation survival rate that improves according to claim 1 is characterized in that the cooling extent of described slow reduction breeding seawater temperature to 5 ℃ is that every 1h is not more than 2 ℃; Described slow reduction breeding seawater temperature to-0.5 ℃ cooling extent is that every 1h is not more than 1 ℃.
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| CN2012101023088A CN102613117A (en) | 2012-04-04 | 2012-04-04 | Method for increasing ice-temperature anhydrous transportation survival rate of turbots |
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| CN2012101023088A CN102613117A (en) | 2012-04-04 | 2012-04-04 | Method for increasing ice-temperature anhydrous transportation survival rate of turbots |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103749340A (en) * | 2013-11-27 | 2014-04-30 | 安徽省郎溪县水产管理站 | Water-free transport method for increasing survival rate of pseudorasbora parva |
| CN106614166A (en) * | 2016-12-29 | 2017-05-10 | 广西还珠海洋生物科技有限公司 | Preparation for improving adaptability of near-river oyster larvae to seawater with big salinity differences |
| CN106614165A (en) * | 2016-12-29 | 2017-05-10 | 广西还珠海洋生物科技有限公司 | Retaining agent capable of improving adaptability of juvenile ostrea rivularis on seawater with large salinity difference |
| CN106614242A (en) * | 2016-12-29 | 2017-05-10 | 广西还珠海洋生物科技有限公司 | Preservation method of seed ostrea rivularis transportation |
| CN106666239A (en) * | 2016-12-29 | 2017-05-17 | 广西还珠海洋生物科技有限公司 | Preservative for transporting young oysters of ostrea rivularis gould |
| CN106665456A (en) * | 2016-12-29 | 2017-05-17 | 广西还珠海洋生物科技有限公司 | Preservation method for ostrea rivularis young oyster transportation |
| CN106689116A (en) * | 2016-12-29 | 2017-05-24 | 广西还珠海洋生物科技有限公司 | Preservative for transporting young ostrea rivularis |
| CN107950636A (en) * | 2017-12-01 | 2018-04-24 | 浙江大学 | A kind of prawn live keeping agent and its method for the transport of prawn waterless keep-alive |
| CN113557999A (en) * | 2021-09-27 | 2021-10-29 | 渤海水产科技(滨州)有限公司 | Water-free alive-keeping transportation method for shrimps |
| CN114145247A (en) * | 2021-11-29 | 2022-03-08 | 浙江海洋大学 | 'gas anesthesia and ecological ice temperature' combined live marine fish transport technology |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103749340A (en) * | 2013-11-27 | 2014-04-30 | 安徽省郎溪县水产管理站 | Water-free transport method for increasing survival rate of pseudorasbora parva |
| CN103749340B (en) * | 2013-11-27 | 2016-01-20 | 安徽省郎溪县水产管理站 | A kind of anhydrous transportation method improving pseudorasbora parva survival rate |
| CN106614166A (en) * | 2016-12-29 | 2017-05-10 | 广西还珠海洋生物科技有限公司 | Preparation for improving adaptability of near-river oyster larvae to seawater with big salinity differences |
| CN106614165A (en) * | 2016-12-29 | 2017-05-10 | 广西还珠海洋生物科技有限公司 | Retaining agent capable of improving adaptability of juvenile ostrea rivularis on seawater with large salinity difference |
| CN106614242A (en) * | 2016-12-29 | 2017-05-10 | 广西还珠海洋生物科技有限公司 | Preservation method of seed ostrea rivularis transportation |
| CN106666239A (en) * | 2016-12-29 | 2017-05-17 | 广西还珠海洋生物科技有限公司 | Preservative for transporting young oysters of ostrea rivularis gould |
| CN106665456A (en) * | 2016-12-29 | 2017-05-17 | 广西还珠海洋生物科技有限公司 | Preservation method for ostrea rivularis young oyster transportation |
| CN106689116A (en) * | 2016-12-29 | 2017-05-24 | 广西还珠海洋生物科技有限公司 | Preservative for transporting young ostrea rivularis |
| CN107950636A (en) * | 2017-12-01 | 2018-04-24 | 浙江大学 | A kind of prawn live keeping agent and its method for the transport of prawn waterless keep-alive |
| CN113557999A (en) * | 2021-09-27 | 2021-10-29 | 渤海水产科技(滨州)有限公司 | Water-free alive-keeping transportation method for shrimps |
| CN113557999B (en) * | 2021-09-27 | 2021-12-21 | 渤海水产科技(滨州)有限公司 | Water-free alive-keeping transportation method for shrimps |
| CN114145247A (en) * | 2021-11-29 | 2022-03-08 | 浙江海洋大学 | 'gas anesthesia and ecological ice temperature' combined live marine fish transport technology |
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Application publication date: 20120801 |