CN114145247A - 'gas anesthesia and ecological ice temperature' combined live marine fish transport technology - Google Patents
'gas anesthesia and ecological ice temperature' combined live marine fish transport technology Download PDFInfo
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- CN114145247A CN114145247A CN202111447827.3A CN202111447827A CN114145247A CN 114145247 A CN114145247 A CN 114145247A CN 202111447827 A CN202111447827 A CN 202111447827A CN 114145247 A CN114145247 A CN 114145247A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 31
- 206010002091 Anaesthesia Diseases 0.000 title claims abstract description 25
- 230000037005 anaesthesia Effects 0.000 title claims abstract description 25
- 238000005516 engineering process Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 13
- 241001596950 Larimichthys crocea Species 0.000 claims description 25
- 239000007789 gas Substances 0.000 claims description 21
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 16
- 239000001569 carbon dioxide Substances 0.000 claims description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 239000013535 sea water Substances 0.000 claims description 8
- 230000003444 anaesthetic effect Effects 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 3
- 230000000241 respiratory effect Effects 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 239000002131 composite material Substances 0.000 abstract description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 102000036675 Myoglobin Human genes 0.000 description 2
- 108010062374 Myoglobin Proteins 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- -1 anesthetic Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a novel marine fish live transportation technology combining gas anesthesia and ecological ice temperature, which complements the advantages of a gas anesthesia method and an ecological ice temperature method, develops a marine fish live transportation composite technology combining the gas anesthesia method and the ecological ice temperature method, which has low cost, low energy consumption, no pollution and high live keeping.
Description
Technical Field
The invention relates to the technical field of marine fish transportation, in particular to a live marine fish transportation technology combining gas anesthesia and ecological ice temperature.
Background
The problems of low survival rate, high difficulty, poor preservation effect of freshness after transportation and the like exist in the live transportation of the marine fishes. At present, the aquatic product market mainly implements the mode of live fish cold chain transportation, and some trade companies often add chemicals such as anesthetic, antibacterial agent to the transportation water for improving the transportation survival rate, reducing the cost of transportation to reduce the mortality of fish, some use even prohibited medicine, brought very big food safety problem. Stress reactions in transport are one of the major causes of death in marine fish. At present, the live-keeping transportation technology of domestic and foreign fishes relieves stress reaction mainly through oxygenation, cooling and anesthesia, reduces fish body damage and energy consumption, but the methods still have the defects of low live-keeping rate, low safety, high cost, low benefit and the like.
Disclosure of Invention
The invention provides a novel live marine fish transportation technology combining gas anesthesia and ecological ice temperature, which can be applied to the transportation of various marine fishes and the biological transportation of aquatic products.
A novel marine fish live transportation technology combining gas anesthesia and ecological ice temperature comprises the following steps:
1) placing the fished sea fish in seawater, and introducing carbon dioxide gas at intervals to make the sea fish enter an anesthetic state at room temperature;
2) slowly cooling to ecological ice temperature of the sea fish;
3) conveying the ecological ice to a destination;
4) when the destination is reached, the temperature is returned to room temperature or oxygen is injected to recover the product.
On the other hand, the invention takes the east China sea large yellow croaker as an object, researches the optimal anesthesia condition and the ecological ice temperature point, and provides a specific live transportation technology combining the gas anesthesia and the ecological ice temperature suitable for the large yellow croaker, which comprises the following steps:
1) putting the caught large yellow croaker into seawater with seawater salinity of 20 per mill, introducing carbon dioxide gas every minute at room temperature for 10s, and repeating the process until the large yellow croaker enters an anesthetic state;
2) slowly cooling to ecological ice temperature of the large yellow croaker;
3) conveying the ecological ice to a destination;
4) when the destination is reached, the temperature is returned to room temperature or oxygen is injected to recover the product.
Preferably, the anesthesia degree in the step 1) is that the body does not move and the gill cover breathes normally.
Preferably, the gas flow rate of carbon dioxide in the step 1) is 2 ppm/s.
Preferably, the cooling rate in step 2) is less than 3 ℃/h, preferably 1-2 ℃/h.
Preferably, the ecological ice temperature in the step 2) is-1.3-8 ℃, and is preferably 8 ℃.
The invention complements the advantages of the gas anesthesia method and the ecological ice temperature method, develops the sea fish live transportation compound technology which combines the gas anesthesia method and the ecological ice temperature method and has low cost, low energy consumption, no pollution and high survival rate.
Drawings
FIG. 1 is a graph comparing the juice loss rate in the examples.
FIG. 2 is a graph comparing pH values in examples.
FIG. 3 is a comparative graph of color difference measurement in examples.
FIG. 4 is a comparative graph of color difference measurement in examples.
FIG. 5 is a graph showing the change in myoglobin in examples.
FIG. 6 is a graph showing the elasticity in the examples.
FIG. 7 is a comparative plot of chewiness in the examples.
FIG. 8 is a graph comparing lactic acid in examples.
FIG. 9 is a comparison of blood glucose measurements before, during and after anesthesia in the examples.
FIG. 10 is a graph comparing the total serum proteins before, during and after anesthesia in the examples.
Detailed Description
The following examples are intended to further illustrate the present invention, but they are not intended to limit or restrict the scope of the invention.
Example 1
The experiment selects Zhejiang boat mountain artificial breeding large yellow croaker (Zhejiang boat mountain peninsula aquaculture company), the large yellow croaker used for the experiment has similar size, similar specification, health and no disease, and good growth situation
1) Putting the caught large yellow croaker into seawater with seawater salinity of 20 per mill, introducing carbon dioxide gas every minute at room temperature for 10s, and repeating the process until the large yellow croaker enters an anesthetic state; the ventilation frequency of carbon dioxide was 2 ppm/s.
2) Slowly cooling to ecological ice temperature of the large yellow croaker of 8 ℃ at 2 ℃/h;
3) ecological ice temperature transportation to a destination (24 h);
4) normal temperature returns to room temperature at the destination;
example 2
The experiment group is divided into a live-transport group (namely, the fish transported by the gas anesthesia ecological ice-temperature composite technology for 24 hours in the embodiment 1 is awakened and then killed), and a control group (namely, the fish killed by oxygen is transported back to the laboratory after being caught in a fishing ground).
Taking large yellow croaker of experimental group and control group, covering fish eyes with cloth to prevent stress reaction, stunning and crushing spinal column to make fish lose its motion ability, bleeding, taking out viscera, and making fish into uniform fish slices of about 1.5 cm/slice for use.
And (3) determining the juice loss rate:
weighing the quality of the fillet and the air-conditioning fresh-keeping bag, and recording as X1Weighing the quality of the freshness protection package, and recording the quality as X2Storing at 4 ℃ in a dark place, taking out the fillets, weighing the quality of the freshness protection package and the residual juice, and recording the quality as X3The following formula is used for calculation.
Juice loss rate X (%) ═ X3-X2)×100％/(X1-X2) The results are shown in FIG. 1, and there is no significant difference between the experimental group and the control group.
And (3) pH value measurement:
the pH value of the large yellow croaker is measured by using a Yingxin AZ8695 pen-touch pH meter, 3 large yellow croaker slices are taken from each group, the pH value of the fixed position of the fish is measured, and the results are shown in figure 2, wherein the change difference between a control group and an experimental group is not obvious.
And (3) measuring color difference:
and (3) measuring the values of L (brightness, whiteness and blackness) and a (red and green) of the thickest back muscle of the large yellow croaker by using a CM-5 type spectrocolorimeter at room temperature. 3 large yellow croakers are taken from each group, data are recorded, and the results are shown in figures 3 and 4, wherein the change difference between the control group and the experimental group is not obvious.
Observation of myoglobin changes:
one large yellow croaker fillet is taken from each group, and the transverse section of the fillet is shot under the environment with similar illumination, proper brightness and no interference, and the shooting is carried out every day for five consecutive days. The results are shown in FIG. 5, and the control group and the experimental group have no significant difference in variation.
And (3) texture determination:
the thickest part of the muscle on the back of the large yellow croaker fillet was measured using a TMS-PRO physical property analyzer (flat-bottomed cylindrical probe diameter 5 mm). The parameters are set as follows: the rising height is 20mm, the minimum initial force is 0.6N, the deformation amount is 30%, the measuring range of the force sensor is 60N, and the probe speed is 60 mm/min. Each group was run in parallel 3 times per 1d measurement. The results of the elasticity comparison are shown in fig. 6, and the results of the chewiness comparison are shown in fig. 7, which indicate that the control group and the experimental group have no significant difference in the change in the elasticity and chewiness.
And (3) measuring the content of lactic acid:
the content of tissue lactic acid is measured by adopting a lactic acid (LD) test box, and the kit is purchased from Nanjing to build a bioengineering institute. The results are shown in FIG. 8. The results show that: the change of the control group and the experimental group is not obvious.
Blood data determination:
the needle tube is pricked into the spinal cord of the live fish to take blood, the blood is divided into two parts, one part is taken as whole blood, and the other part is centrifuged by a centrifuge to take serum. And then the examination is carried out. The results of blood glucose vs. e.g. fig. 9 and serum total protein vs. e.g. fig. 10 show that: the difference between the change before and after anesthesia is not obvious.
Claims (6)
1. A novel marine fish live transportation technology combining gas anesthesia and ecological ice temperature is characterized by comprising the following steps:
1) placing the fished sea fish in seawater, and introducing carbon dioxide gas at intervals to make the sea fish enter an anesthetic state at room temperature;
2) slowly cooling to ecological ice temperature of the sea fish;
3) conveying the ecological ice to a destination;
4) when the destination is reached, the temperature is returned to room temperature or oxygen is injected to recover the product.
2. A live transportation technology combining gas anesthesia and ecological ice temperature of large yellow croakers is characterized by comprising the following steps:
1) putting the caught large yellow croaker into seawater with seawater salinity of 20 per mill, introducing carbon dioxide gas every minute at room temperature for 10s, and repeating the process until the large yellow croaker enters an anesthetic state;
2) slowly cooling to ecological ice temperature of the large yellow croaker;
3) conveying the ecological ice to a destination;
4) when the destination is reached, the temperature is returned to room temperature or oxygen is injected to recover the product.
3. The combination of gas anesthesia and ecological ice temperature for large yellow croaker according to claim 1 or 2, wherein the anesthesia level in step 1) is no body movement and normal respiratory gill cover.
4. The live transportation technology of large yellow croaker combining gas anesthesia and ecological ice temperature as claimed in claim 2, wherein the gas flow rate of carbon dioxide in step 1) is 2 ppm/s.
5. The live transportation technology of large yellow croaker combining gas anesthesia with ecological ice temperature as claimed in claim 2, wherein the cooling rate in step 2) is less than 3 ℃/h, preferably 1-2 ℃/h.
6. The transportation technique of large yellow croaker combining "gas anesthesia and ecological ice temperature" as claimed in claim 2, wherein the ecological ice temperature in step 2) is-1.3-8 ℃, preferably 8 ℃.
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| CN202111447827.3A CN114145247A (en) | 2021-11-29 | 2021-11-29 | 'gas anesthesia and ecological ice temperature' combined live marine fish transport technology |
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| CN202111447827.3A CN114145247A (en) | 2021-11-29 | 2021-11-29 | 'gas anesthesia and ecological ice temperature' combined live marine fish transport technology |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008054559A (en) * | 2006-08-30 | 2008-03-13 | Hayashikane Sangyo Kk | Method for anesthetizing fish and anesthetizing device |
| CN102613117A (en) * | 2012-04-04 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | Method for increasing ice-temperature anhydrous transportation survival rate of turbots |
| CN106719196A (en) * | 2016-12-08 | 2017-05-31 | 广东海洋大学 | A kind of waterless keep-alive transportation resources of Environment of Litopenaeus vannamei Low |
| CN108477031A (en) * | 2018-03-20 | 2018-09-04 | 广东海洋大学 | A kind of anhydrous transportation method using cold carbon dioxide narcosis seawater grouper |
| CN111937786A (en) * | 2020-08-26 | 2020-11-17 | 上海海洋大学 | Ecological ice-temperature water-free keep-alive method for lateolabrax japonicus |
| CN112772534A (en) * | 2021-01-11 | 2021-05-11 | 浙江海洋大学 | Keep-alive transportation method for large aquatic products |
-
2021
- 2021-11-29 CN CN202111447827.3A patent/CN114145247A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008054559A (en) * | 2006-08-30 | 2008-03-13 | Hayashikane Sangyo Kk | Method for anesthetizing fish and anesthetizing device |
| CN102613117A (en) * | 2012-04-04 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | Method for increasing ice-temperature anhydrous transportation survival rate of turbots |
| CN106719196A (en) * | 2016-12-08 | 2017-05-31 | 广东海洋大学 | A kind of waterless keep-alive transportation resources of Environment of Litopenaeus vannamei Low |
| CN108477031A (en) * | 2018-03-20 | 2018-09-04 | 广东海洋大学 | A kind of anhydrous transportation method using cold carbon dioxide narcosis seawater grouper |
| CN111937786A (en) * | 2020-08-26 | 2020-11-17 | 上海海洋大学 | Ecological ice-temperature water-free keep-alive method for lateolabrax japonicus |
| CN112772534A (en) * | 2021-01-11 | 2021-05-11 | 浙江海洋大学 | Keep-alive transportation method for large aquatic products |
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