CN111937786A - Ecological ice-temperature water-free keep-alive method for lateolabrax japonicus - Google Patents
Ecological ice-temperature water-free keep-alive method for lateolabrax japonicus Download PDFInfo
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- CN111937786A CN111937786A CN202010866588.4A CN202010866588A CN111937786A CN 111937786 A CN111937786 A CN 111937786A CN 202010866588 A CN202010866588 A CN 202010866588A CN 111937786 A CN111937786 A CN 111937786A
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- 241000269795 Lateolabrax japonicus Species 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 23
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 21
- 241000251468 Actinopterygii Species 0.000 claims abstract description 18
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 10
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 238000004806 packaging method and process Methods 0.000 claims abstract description 7
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000001301 oxygen Substances 0.000 claims abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 5
- 241000234314 Zingiber Species 0.000 claims description 8
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 8
- 235000008397 ginger Nutrition 0.000 claims description 8
- 230000000384 rearing effect Effects 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 6
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 5
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 claims description 5
- 239000000600 sorbitol Substances 0.000 claims description 5
- 102000016943 Muramidase Human genes 0.000 claims description 4
- 108010014251 Muramidase Proteins 0.000 claims description 4
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 4
- XHCADAYNFIFUHF-TVKJYDDYSA-N esculin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC2=C1OC(=O)C=C2 XHCADAYNFIFUHF-TVKJYDDYSA-N 0.000 claims description 4
- 239000004325 lysozyme Substances 0.000 claims description 4
- 229960000274 lysozyme Drugs 0.000 claims description 4
- 235000010335 lysozyme Nutrition 0.000 claims description 4
- OKTJSMMVPCPJKN-YPZZEJLDSA-N carbon-10 atom Chemical compound [10C] OKTJSMMVPCPJKN-YPZZEJLDSA-N 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000006260 foam Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 3
- 208000027418 Wounds and injury Diseases 0.000 claims description 2
- 230000006378 damage Effects 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 208000014674 injury Diseases 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000005059 dormancy Effects 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000004060 metabolic process Effects 0.000 abstract description 3
- 230000000241 respiratory effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 239000002023 wood Substances 0.000 abstract description 2
- 239000013505 freshwater Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 241000276573 Cottidae Species 0.000 description 2
- 241000269794 Lateolabrax Species 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 241000276618 Perciformes Species 0.000 description 1
- 241001417495 Serranidae Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D77/00—Packages formed by enclosing articles or materials in preformed containers, e.g. boxes, cartons, sacks or bags
- B65D77/04—Articles or materials enclosed in two or more containers disposed one within another
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
- B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
- B65D81/2069—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas in a special atmosphere
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
- Evolutionary Biology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
An ecological ice-temperature anhydrous survival method for lateolabrax japonicus is characterized in that the lateolabrax japonicus is subjected to short-term fasting temporary culture, the respiratory metabolism rate of the lateolabrax japonicus can be properly reduced, then the water temperature is reduced to the dormancy temperature of the lateolabrax japonicus at a proper cooling rate, the lateolabrax japonicus is adapted for a period of time under a dormancy water environment, finally, oxygen is filled, carbon dioxide and nitrogen are packaged, the lateolabrax japonicus is subjected to anhydrous survival at a low temperature, the lateolabrax japonicus can survive for 8 hours under an anhydrous state, the lateolabrax japonicus is subjected to anhydrous survival at a temperature of 4 ℃ in the water environment, the temperature rise rate is 5 ℃/h. The method replaces wet wood chip packaging with the live-keeping liquid packaging, reduces the volume of the container, reduces the transportation weight, effectively reduces the transportation cost, is particularly suitable for express delivery and air transportation, improves the waterless live transportation effect of the marine fishes, and obtains higher economic benefit.
Description
Technical Field
The invention relates to the field of aquatic product keep-alive, in particular to a waterless keep-alive method for seawater fish, and particularly relates to a waterless keep-alive method for lateolabrax japonicus.
Background
Lateolabrax maculosus belongs to the class of Pisces, Perciformes, Serranidae, Oligoriaceae and Lateolabrax, and is different from fresh water bass in large and long body type, rough scales, lower jaw longer than upper jaw and sharp mouth. Lateolabrax japonicus belongs to euryhaline fishes, is fond of inhabiting in the saline water at the river mouth and can live in the fresh water. The fresh water is produced in fresh water bodies along the sea and through the sea in China, and the east sea and the Bohai sea are more. The main producing areas are Qingdao, Shidao, Qinhuang island and Zhoushan island. The fishing period is spring and autumn, and 10-11 months per year are full fishing periods. The lateolabrax japonicus has delicious meat and higher nutritional value than fresh water lateolabrax japonicus, and is one of common marine economic fishes.
Due to the reasons of large span of production and marketing regions, inaccurate keep-alive technology, imperfect configuration of transportation equipment and the like, the problems of high transportation cost, large loss rate (more than 10%) of long-distance transportation and the like of the marine fishes are caused, and the development of the marine fish sales market is greatly restricted. The waterless keep-alive method is used as a novel marine fish transportation mode and has the advantages of low cost, low loss and the like.
Through reference of documents and actual investigation, the application of the largemouth sculpin to the waterless keep-alive is not found, so that the invention makes up the application of the largemouth sculpin to the field of the waterless keep-alive transportation of the marine fishes.
Disclosure of Invention
The invention aims to solve the technical problem of improving a waterless keep-alive process of seawater fish and providing an implementation and use method thereof.
The technical problem to be solved by the invention is realized by the following technical measures:
the invention is characterized by comprising the following specific operation steps:
(1) temporary culture: fasting and temporarily culturing the lateolabrax japonicus for 6 hours before keeping the lateolabrax japonicus alive without water; the concentration of vitamin C1 in the temporary rearing pond is 25 mg/L, and the concentration of vitamin C2 in the temporary rearing pond is 30 mg/L.
(2) Cold domestication: reducing the water temperature from 22-23 ℃ to 4 ℃ at a cooling rate of 3 ℃/h, and temporarily culturing at 4 ℃ for 0-8 h;
(3) coating with a keep-alive liquid: fishing out the fish with very weak touch reaction in the temporary rearing box, and quickly and uniformly smearing the fish body with the keep-alive liquid for 2-3 times; placing in a foam box of 10 cm × 15 cm × 13 cm, with the ventral fin of the lateolabrax japonicus as the lower dorsal fin; packing the mixture into 1 strip/box, packing the mixture into plastic bags, charging oxygen, carbon dioxide and nitrogen, tightening the bag openings, placing the bags into a low-temperature refrigerated transport vehicle, and maintaining the constant temperature of 4 ℃ in a carriage.
(4) And (3) refrigerating and transporting: the temperature of the carriage was maintained at 4 ℃ during transportation.
(5) And (4) awakening: after the water-free keep-alive, the lateolabrax japonicus is moved to a water environment with the temperature of 4 ℃ and is heated to the environmental temperature at the heating rate of 5 ℃/h.
The lateolabrax japonicus should be selected from fish with uniform size, health, no injury, and vitality.
In one embodiment, the keep-alive solution consists of: 0.08 to 0.1 percent of lysozyme; sorbitol 0.2-0.4%, ginger liquid 0.6-1%, and water in balance. The salinity of the keep-alive liquid is 16-17 per mill, and the pH value is 7.5-8.5.
In one embodiment, the ginger liquid is prepared by: cleaning rhizoma Zingiberis recens, draining, adding 150 mL distilled water per kg raw material, mixing, squeezing, filtering with 200 mesh gauze, sterilizing, and cooling to 4 deg.C.
In one embodiment, the oxygen, carbon dioxide and nitrogen gas filled in the plastic bag consists of: 80% of oxygen, 10% of carbon dioxide and 10% of nitrogen.
Preferably, the keep-alive liquid is 0.09% + sorbitol 0.3% + ginger liquid 0.08%.
The method replaces wet wood chip packaging with the live-keeping liquid packaging, reduces the volume of the container, reduces the transportation weight, effectively reduces the transportation cost, is particularly suitable for express delivery and air transportation, improves the waterless live transportation effect of the marine fishes, and obtains higher economic benefit. The method is characterized in that the lateolabrax japonicus is temporarily fasted for a short time, the respiratory metabolism rate of the lateolabrax japonicus can be properly reduced, the water temperature is reduced to the dormancy temperature of the lateolabrax japonicus at a proper temperature reduction rate, the lateolabrax japonicus is adapted for a period of time under a dormancy water environment, finally, the water is oxygenated, the carbon dioxide and the nitrogen are packaged and keep alive without water at a low temperature, the lateolabrax japonicus can survive for 8 hours under a water-free state, the survival rate of the lateolabrax japonicus in 1 day can reach 100 percent after the lateolabrax japonicus is kept alive without water and the temperature is increased to.
Detailed Description
In order to make the operational procedures and the creative features of the implementation of the invention easy to understand, the invention is further explained below by combining the specific embodiments.
The lateolabrax japonicus is transported to a temporary culture pond in a laboratory from the market of the previous seawater products for 2 hours at the water temperature of 15 ℃ and the transport density of 0.2 kg/L; after two days of fasting, feeding for 10 days, and feeding for 2 times every day, wherein the daily exposure to normal light is provided, and the oxygen supply amount is 5 mg/min.
Selecting healthy, harmless and viable lateolabrax japonicus with uniform size for keeping alive without water, and fasting and temporarily culturing for 6h before experiment. Vitamin C1 was added at 25 mg/L and vitamin C2 was added at 30mg/L to the pond.
(1) Temporary culture: fasting and temporarily culturing the lateolabrax japonicus for 6 hours before keeping the lateolabrax japonicus alive without water; vitamin C2 was added to the pond at 30 mg/L.
(2) Cold domestication: putting 15 lateolabrax japonicus into a water tank filled with 150L of water, reducing the water temperature from 22-23 ℃ to 4 ℃ of critical temperature according to the cooling rate of 3 ℃/h, and adapting for 6h at 4 ℃.
(3) Coating with a keep-alive liquid: fishing out the fish with very weak touch reaction in the temporary rearing box, and quickly and uniformly smearing the fish body with the keep-alive liquid for 2-3 times; placing in a foam box of 10 cm × 15 cm × 13 cm, with the ventral fin of the lateolabrax japonicus as the lower dorsal fin; packing the mixture into a plastic bag with the density of 1 strip/box, filling oxygen, carbon dioxide and nitrogen into the plastic bag, wherein the gas components are as follows: 80% of oxygen, 10% of carbon dioxide and 10% of nitrogen, tightening the bag opening, putting the bag into a low-temperature refrigerated transport vehicle, and maintaining the constant temperature of 4 ℃ in a carriage. The keep-alive liquid is 0.09% of lysozyme, 0.3% of sorbitol, 0.08% of ginger liquid, 16% of salinity of the keep-alive liquid and 8 of pH value. The preparation method of the ginger liquid comprises the following steps: cleaning rhizoma Zingiberis recens, draining, adding 150 mL distilled water per kg raw material, mixing, squeezing, filtering with 200 mesh gauze, sterilizing, and cooling to 4 deg.C.
(4) And (3) refrigerating and transporting: the temperature of the carriage is maintained at 4 ℃ in the transportation process, and the carriage is transported for 8 hours.
(5) And (4) awakening: after the water-free keep-alive, the lateolabrax japonicus is moved to a water environment with the temperature of 4 ℃ and is heated to the environmental temperature at the heating rate of 5 ℃/h.
The method comprises the steps of temporarily raising the lateolabrax japonicus in a short period of fasting to properly reduce the respiratory metabolism rate, then reducing the water temperature to the dormancy temperature of the lateolabrax japonicus at a proper temperature reduction rate, adapting to a dormant water environment for a period of time, finally carrying out oxygenation, carbon dioxide and nitrogen packaging and keeping alive without water at a low temperature, wherein the lateolabrax japonicus can survive for 8 hours in a water-free state, and after keeping alive without water, the survival rate of the lateolabrax japonicus in 1 day can reach 100 percent after the lateolabrax japonicus is recovered to normal in the water environment at the temperature rise rate of 5 ℃/h at the temperature of 4 ℃.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (6)
1. The ecological ice-temperature waterless keep-alive method for the lateolabrax japonicus is characterized by comprising the following steps: the specific operation steps are as follows:
(1) temporary culture: fasting and temporarily culturing the lateolabrax japonicus for 6 hours before keeping the lateolabrax japonicus alive without water; the concentration of vitamin C1 in the temporary rearing pond is 25 mg/L, and the concentration of vitamin C2 in the temporary rearing pond is 30 mg/L;
(2) cold domestication: reducing the water temperature from 22-23 ℃ to 4 ℃ at a cooling rate of 3 ℃/h, and temporarily culturing at 4 ℃ for 0-8 h;
(3) coating with a keep-alive liquid: fishing out the fish with very weak touch reaction in the temporary rearing box, and quickly and uniformly smearing the fish body with the keep-alive liquid for 2-3 times; placing in a foam box of 10 cm × 15 cm × 13 cm, with the ventral fin of the lateolabrax japonicus as the lower dorsal fin; packaging into 1 strip/box, packaging into plastic bag, charging oxygen, carbon dioxide and nitrogen, tightening bag opening, placing into low temperature refrigerated transport vehicle, and maintaining constant temperature of 4 deg.C in carriage;
(4) and (3) refrigerating and transporting: the temperature of the carriage is maintained at 4 ℃ in the transportation process;
(5) and (4) awakening: after the water-free keep-alive, the lateolabrax japonicus is moved to a water environment with the temperature of 4 ℃ and is heated to the environmental temperature at the heating rate of 5 ℃/h.
2. The ecological ice-temperature water-free keep-alive method for lateolabrax japonicus according to claim 1, which is characterized in that: the lateolabrax japonicus should be selected from fish with uniform size, health, no injury, and vitality.
3. The ecological ice-temperature water-free keep-alive method for lateolabrax japonicus according to claim 1, which is characterized in that: the keep-alive liquid comprises the following components: 0.08 to 0.1 percent of lysozyme; sorbitol 0.2% -0.4%, ginger liquid 0.6% -1%, and the balance of water; the salinity of the keep-alive liquid is 16-17 per mill, and the pH value is 7.5-8.5.
4. The ecological ice-temperature water-free keep-alive method for lateolabrax japonicus according to claim 1, which is characterized in that: the preparation of the ginger liquid comprises the following steps: cleaning rhizoma Zingiberis recens, draining, adding 150 mL distilled water per kg raw material, mixing, squeezing, filtering with 200 mesh gauze, sterilizing, and cooling to 4 deg.C.
5. The ecological ice-temperature water-free keep-alive method for lateolabrax japonicus according to claim 1, which is characterized in that: the oxygen, carbon dioxide and nitrogen filled in the plastic bag comprise the following components: 80% of oxygen, 10% of carbon dioxide and 10% of nitrogen.
6. The ecological ice-temperature water-free keep-alive method for lateolabrax japonicus according to claim 1, which is characterized in that: the keep-alive liquid is 0.09% of lysozyme, 0.3% of sorbitol and 0.08% of ginger liquid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145247A (en) * | 2021-11-29 | 2022-03-08 | 浙江海洋大学 | 'gas anesthesia and ecological ice temperature' combined live marine fish transport technology |
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2020
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| CN109287527A (en) * | 2018-09-26 | 2019-02-01 | 中国海洋大学 | A method of it studying flower perch juvenile fish temperature and is resistant to feature |
| CN109548714A (en) * | 2018-12-03 | 2019-04-02 | 上海海洋大学 | A kind of anhydrous transportation method of fish |
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