CN101940174A - Method for anhydrously surviving freshwater fish through repeated dormancy - Google Patents
Method for anhydrously surviving freshwater fish through repeated dormancy Download PDFInfo
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- CN101940174A CN101940174A CN2010102417463A CN201010241746A CN101940174A CN 101940174 A CN101940174 A CN 101940174A CN 2010102417463 A CN2010102417463 A CN 2010102417463A CN 201010241746 A CN201010241746 A CN 201010241746A CN 101940174 A CN101940174 A CN 101940174A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses a method for anhydrously surviving freshwater fish through repeated dormancy, at least comprising two times of dormancy. The times of the repeated dormancy are selected according to the practical needs of transportation distance and time. The method comprises the following steps of: temporary culture, first dormancy, recovery convalesce, second dormancy and convalesce. During the transportation of marine products, repeated dormancy survival is carried out on the same batch of live fishes to prolong the anhydrous survival time and be beneficial to the living body transportation of the live fishes.
Description
Technical field
The present invention relates to the method for keeping fresh water fish alive without water, be specifically related to a kind of method of the keeping fresh water fish alive without water of dormancy repeatedly.
Background technology
As everyone knows, a segment distance is often arranged between the place of production of aquatic products and the area of consumption, live fish keep-alive transportation has become aquatic products circulation important key link, is the effective means that keeps the best freshness of aquatic products, satisfies consumer demand.Because the live fish leaving water is easy to death, existing live fish transportation needs to place in the container that is filled with water live fish and oxygenation in real time.But the fish transportation vehicle that is equipped with oxygen cylinder does not allow to pass through the tunnel; If in driving process, bring to a halt, the fish transportation vehicle that causes fully loaded live fish because of the impulse force (acceleration) of water between the moment gross weight increase serious overload rapidly; In case the container breakage is leaked, or oxygen supply is obstructed, and can cause the death of fish body; A large amount of water can bring inconvenience in transportation, during the live fish transportation, the fish body often gives off more ammonia and mucus polluted water because of terrified or anoxic, because the fish density in the water is bigger, the strenuous exercise of fish in water environment also can make the fish body injured in the transportation; Add that the collision between fish and fish, fish and wall can damage the fish body again, cause to have the death of a large amount of fish body after arriving the destination, give culture, the transportation family causes economic loss; If the fish body of dying is dealt with improperly, also can cause environmental pollution.
The fish waterless keep-alive is an emerging technology, and it is to make fish be in resting state under the state of anhydrous hypoxemia, thereby reach the time-to-live that prolongs fish, reaches the fresh-keeping purpose of keep-alive.The waterless keep-alive technology mainly adopts means such as low temperature, ancillary drug, makes the fish body enter that metabolism intensity is low, oxygen consumption is few, the resting state of bradykinesia, prolongs time-to-live and the survival rate of fresh-water fishes under anhydrous state.In recent years, the waterless keep-alive technology is owing to have that carrying capacity is big, survival rate is high, cost is low, be convenient to advantage such as management, and living at aquatic products obtains more and more widely utilization in transporting.Owing to there is not the existence of water in the transportation, the survival rate that so just can improve fish can reduce the cost in the transportation again greatly.Existing publication and pertinent literature report respectively fresh water and salt water product are implemented dormancy anesthesia keep-alive, but these data all do not relate to dormancy repeatedly.Many times, aquatic products need just can transport the sales territory to through transhipment, need in the way same mass products fish is carried out repeatedly long-time keep-alive transportation.
Summary of the invention
The objective of the invention is to: a kind of method of the keeping fresh water fish alive without water of dormancy repeatedly is provided, in transit same mass products fish is carried out repeatedly dormancy keep-alive repeatedly, prolong the waterless keep-alive time, help the live transport of fresh-water fishes at aquatic products.
Technical solution of the present invention is: dormancy waterless keep-alive method comprises twice dormancy at least repeatedly, and the number of times of dormancy is selected according to the actual needs of transportation range and time repeatedly; The step of this method comprise temporarily support, dormancy for the first time, recovery are rested, dormancy for the second time and recovery; Concrete steps are: at first, that fish for or the commercially available fresh-water fishes that are going strong place clear water to support a few hours temporarily, and keep the cleaning of water; Secondly, change live fish over to edible alcohol or liquor contains ethanol dilution liquid, treat that live fish enters resting state after, break away from water environment, place the container of moist environment to transport; Then, as required, the live fish that will be in after a few hours in the dormancy places water to recover, treat fish recover normal after, in clear water, rest a few hours; Then, the live fish after resting changes edible alcohol once more over to or liquor contains ethanol dilution liquid, according to the method dormancy once more of the dormancy first time; At last, recover in the clear water; Wherein, supported the time temporarily and be in the clear water 20～50 hours, make fish on an empty stomach; Wherein, supporting water temporarily is through air-pump inflating running water or the pond water of 24h at least; Wherein, the dormancy time under the anhydrous state first time was at 5～12 hours; Wherein, the recovery time of resting is 5～50 hours, eliminates the interior a spot of alcohol residue of fish body that last time dormancy produces, and makes live fish recover normal physiological function; Wherein, the dormancy time under the anhydrous state second time was at 5～12 hours; Wherein, edible alcohol or the liquor pure content that contains ethanol dilution liquid is implemented according to publication " method of keeping fresh water fish alive without water " CN101107922A; Wherein, support temporarily, recover to rest and at room temperature implement, the temperature of dormancy transportation is at 5～20 ℃.
Wherein, when transhipment needs more than twice dormancy repeatedly, last time dormancy and the recovery time of resting between dormancy is 8～20 hours once more.
The present invention has the following advantages:
1, the time of supporting temporarily is in the clear water 20～50 hours, makes fish on an empty stomach, reduces or avoids a small amount of ight soil residual in the fish belly influence time of waterless keep-alive, effectively prolongs the keep-alive time, raising keep-alive rate;
2, in order effectively to improve the survival rate of fish, prolong the keep-alive time, anhydrous state under the first time dormancy time at 5～12 hours, specifically the time along with the difference of the kind of fish difference;
3, the recovery time of resting is 5～50 hours, eliminates a spot of alcohol residue in the issuable fish body of last time dormancy, makes live fish recover normal physiological function; When transportation or transhipment need more than twice dormancy repeatedly, last time dormancy and once more the time of resting of the recovery between dormancy preferable with 8～20 hours effects;
4, the present invention with more than twice or twice repeatedly dormancy be characteristics, select for use edible alcohol or liquor to contain ethanol dilution liquid as dormancy inducer, impel live fish to enter resting state at normal temperatures and pressures, edible alcohol or liquor safety non-toxic, and the diluent concentration that is used for the live fish dormancy is very low, safety, convenient, easily accepted by the consumer;
5, dormancy is compared with the single dormancy there is not influence substantially by the fresh-water fishes quality of keep-alive repeatedly, twice or twice above dormancy keep-alive, and its waterless keep-alive time obviously prolongs, and helps the live transport of fresh-water fishes more.
Description of drawings
Fig. 1 is twice dormancy waterless keep-alive process lactic acid content changing trend diagram
Fig. 2 is twice dormancy waterless keep-alive process lactate dehydrogenase content figure
Embodiment
Further specify technical solution of the present invention below in conjunction with specific embodiment, these embodiment can not be interpreted as it is restriction to technical solution.
Embodiment 1:
Select for use Huai rice and fuel river valley crucian some, average body length is 15.5 ± 1.5cm, and average weight is 54.2 ± 10.0g, and physique is healthy and strong active, and size is even, and scale is complete, healthy no disease; At first, room temperature is stopped eating and is supported temporarily 50h, supports water temporarily and is through the air-pump inflating running water of 24h at least; Subsequently, live fish is changed in the edible alcohol dilution over to induced dormancy, waterless keep-alive 12h under 5 ℃ of environment; Wherein, to contain the pure content of ethanol dilution liquid be to contain ethanol dilution liquid among publication " method of the keeping fresh water fish alive without water " CN101107922A to edible alcohol; Then, recover in the room temperature clear water, 50h rests; Next changes over to induce in the edible alcohol dilution and implements dormancy for the second time, waterless keep-alive 12h under 5 ℃ of environment; At last, room temperature is recovered in clear water.
Adopt reverse high performance liquid chromatography, measure the variation of twice keep-alive process lactic acid content, the variation tendency that the results are shown in Figure lactic acid content in 1, the twice keep-alive process is the basically identical time of advent of peak particularly.
Adopt determined by ultraviolet spectrophotometry lactate dehydrogenase (LDH) content, the results are shown in Figure the variation tendency basically identical of LDH content in 2, the twice keep-alive processes.
Lactic acid is the end-product of glycolytic pathway.When the body anoxic, cell generates ATP by anaerobic metabolism.For the enough NAD of energy regeneration
+, carbohydrate metabolism is continued, lactate dehydrogenase is reduced to lactic acid by the pyruvic acid that glycolytic pathway is produced, and makes the NADH oxidation.The lactic acid of muscle accumulation is released in the blood circulation during histanoxia, passes through liver metabolism.
Embodiment 2:
Select for use Huai rice and fuel river valley crucian some, crucian carp fish length 10～12cm, body weight 100～150g; At first, stop eating in the room temperature clear water and supported 35 hours temporarily, support water temporarily and be through the air-pump inflating running water of 24h at least; Subsequently, live fish is changed in the liquor dilution over to induced dormancy, waterless keep-alive 8h under 12 ℃ of environment; Wherein, to contain the pure content of ethanol dilution liquid be to contain ethanol dilution liquid among publication " method of the keeping fresh water fish alive without water " CN101107922A to liquor; Then, room temperature is recovered in clear water, rests 27 hours; Next changes over to induce in the liquor dilution and implements dormancy for the second time, waterless keep-alive 8h under 12 ℃ of environment; At last, room temperature is recovered in clear water.
So repeated multiple times is induced timing certainly first, and the limit time-to-live of crucian can reach 126 hours.Before and after the keep-alive, it is extremely small that flesh of fish ash value, the flesh of fish are that indexs such as waterpower, flesh of fish fat content and fish meat protein content change, and shows that dormancy repeatedly do not have influence substantially to the quality characteristic of crucian.
Embodiment 3:
Select for use Huai rice and fuel river valley crucian some, crucian carp fish length 10～12cm, body weight 100～150g; At first, stop eating in the room temperature clear water and supported 20 hours temporarily, support water temporarily and be through the air-pump inflating running water of 24h at least; Subsequently, live fish is changed in the liquor dilution over to induced dormancy, waterless keep-alive 5h under 20 ℃ of environment; Wherein, to contain the pure content of ethanol dilution liquid be to contain ethanol dilution liquid among publication " method of the keeping fresh water fish alive without water " CN101107922A to liquor; Then, room temperature is recovered in clear water, rests 5 hours; Next changes over to induce in the liquor dilution and implements dormancy for the second time, waterless keep-alive 5h under 20 ℃ of environment; At last, room temperature is recovered in clear water.
Embodiment 4:
Select for use Huai rice and fuel river valley crucian some, average body length is 15.5 ± 1.5cm, and average weight is 54.2 ± 10.0g, and physique is healthy and strong active, and size is even, and scale is complete, healthy no disease; At first, room temperature is stopped eating and is supported temporarily 50h, supports water temporarily and is through the air-pump inflating running water of 24h at least; Subsequently, live fish is changed in the edible alcohol dilution over to induced dormancy, waterless keep-alive 12h under 5 ℃ of environment; Wherein, to contain the pure content of ethanol dilution liquid be to contain ethanol dilution liquid among publication " method of the keeping fresh water fish alive without water " CN101107922A to edible alcohol; Then, recover in the room temperature clear water, 20h rests; Next changes over to induce in the edible alcohol dilution and implements dormancy for the second time, waterless keep-alive 12h under 5 ℃ of environment; At last, room temperature is recovered in clear water.
Embodiment 5:
Select for use Huai rice and fuel river valley crucian some, crucian carp fish length 10～12cm, body weight 100～150g; At first, stop eating in the room temperature clear water and supported 35 hours temporarily, support water temporarily and be through the air-pump inflating running water of 24h at least; Subsequently, live fish is changed in the liquor dilution over to induced dormancy, waterless keep-alive 8h under 12 ℃ of environment; Wherein, to contain the pure content of ethanol dilution liquid be to contain ethanol dilution liquid among publication " method of the keeping fresh water fish alive without water " CN101107922A to liquor; Then, room temperature is recovered in clear water, rests 14 hours; Next changes over to induce in the liquor dilution and implements dormancy for the second time, waterless keep-alive 8h under 12 ℃ of environment; At last, room temperature is recovered in clear water.
Embodiment 6:
Select for use Huai rice and fuel river valley crucian some, crucian carp fish length 10～12cm, body weight 100～150g; At first, stop eating in the room temperature clear water and supported 20 hours temporarily, support water temporarily and be through the air-pump inflating running water of 24h at least; Subsequently, live fish is changed in the liquor dilution over to induced dormancy, waterless keep-alive 5h under 20 ℃ of environment; Wherein, to contain the pure content of ethanol dilution liquid be to contain ethanol dilution liquid among publication " method of the keeping fresh water fish alive without water " CN101107922A to liquor; Then, room temperature is recovered in clear water, rests 8 hours; Next changes over to induce in the liquor dilution and implements dormancy for the second time, waterless keep-alive 5h under 20 ℃ of environment; At last, room temperature is recovered in clear water.
Claims (2)
1. the method for dormancy keeping fresh water fish alive without water repeatedly, it is characterized in that: dormancy waterless keep-alive method comprises twice dormancy at least repeatedly, the number of times of dormancy is selected according to the actual needs of transportation range and time repeatedly; The step of this method comprise temporarily support, dormancy for the first time, recovery are rested, dormancy for the second time and recovery; Concrete steps are: at first, that fish for or the commercially available fresh-water fishes that are going strong place clear water to support a few hours temporarily, and keep the cleaning of water; Secondly, change live fish over to edible alcohol or liquor contains ethanol dilution liquid, treat that live fish enters resting state after, break away from water environment, place the container of moist environment to transport; Then, as required, the live fish that will be in after a few hours in the dormancy places water to recover, treat fish recover normal after, in clear water, rest a few hours; Then, change edible alcohol once more over to after resting or liquor contains ethanol dilution liquid, according to the method dormancy once more of the dormancy first time; At last, recover in the clear water; Wherein, supported the time temporarily and be in the clear water 20～50 hours, make fish on an empty stomach; Wherein, supporting water temporarily is through air-pump inflating running water or the pond water of 24h at least; Wherein, the dormancy time under the anhydrous state first time was at 5～12 hours; Wherein, the recovery time of resting is 5～50 hours, eliminates the interior a spot of alcohol residue of fish body that last time dormancy produces, and makes live fish recover normal physiological function; Wherein, the dormancy time under the anhydrous state second time was at 5～12 hours; Wherein, edible alcohol or the liquor pure content that contains ethanol dilution liquid is implemented according to publication " method of keeping fresh water fish alive without water " CN101107922A; Wherein, support temporarily, recover to rest and at room temperature implement, the temperature of dormancy transportation is at 5～20 ℃.
2. the method for the keeping fresh water fish alive without water of dormancy repeatedly according to claim 1 is characterized in that: wherein, when transhipment needs more than twice dormancy repeatedly, last time dormancy and the recovery time of resting between dormancy is 8～20 hours once more.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102417463A CN101940174A (en) | 2010-07-30 | 2010-07-30 | Method for anhydrously surviving freshwater fish through repeated dormancy |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102417463A CN101940174A (en) | 2010-07-30 | 2010-07-30 | Method for anhydrously surviving freshwater fish through repeated dormancy |
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| CN101940174A true CN101940174A (en) | 2011-01-12 |
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| CN2010102417463A Pending CN101940174A (en) | 2010-07-30 | 2010-07-30 | Method for anhydrously surviving freshwater fish through repeated dormancy |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706981A (en) * | 2016-03-07 | 2016-06-29 | 五河县金满塘生态种养殖家庭农场 | Fresh water live fish transportation management method |
| CN108887206A (en) * | 2018-06-15 | 2018-11-27 | 施乐谦 | A kind of freshwater fish waterless keep-alive transportation resources |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1027567C (en) * | 1993-05-07 | 1995-02-08 | 李振瑜 | Reservation and transportation method for living aquatic product |
| JPH11341934A (en) * | 1998-06-01 | 1999-12-14 | Nihon Nosan Kogyo Kk | Transportation and maintenance of fish and shellfish and prolongation of hatching period of embryonic egg |
| CN1669413A (en) * | 2004-03-19 | 2005-09-21 | 中国水产科学研究院黄海水产研究所 | Marine finishes life preserving method without water |
| CN101107922A (en) * | 2007-08-13 | 2008-01-23 | 淮阴工学院 | Method of keeping fresh water fish alive without water |
-
2010
- 2010-07-30 CN CN2010102417463A patent/CN101940174A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1027567C (en) * | 1993-05-07 | 1995-02-08 | 李振瑜 | Reservation and transportation method for living aquatic product |
| JPH11341934A (en) * | 1998-06-01 | 1999-12-14 | Nihon Nosan Kogyo Kk | Transportation and maintenance of fish and shellfish and prolongation of hatching period of embryonic egg |
| CN1669413A (en) * | 2004-03-19 | 2005-09-21 | 中国水产科学研究院黄海水产研究所 | Marine finishes life preserving method without water |
| CN101107922A (en) * | 2007-08-13 | 2008-01-23 | 淮阴工学院 | Method of keeping fresh water fish alive without water |
Non-Patent Citations (2)
| Title |
|---|
| 《江苏农业科学》 20100430 张恒等 食用酒精对淡水鱼无水保活保鲜品质的影响 第297-300页 1-2 , 第2期 2 * |
| 《河北渔业》 20091231 黄海等 水产品保活运输技术研究进展 第45-47页 1-2 , 第9期 2 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706981A (en) * | 2016-03-07 | 2016-06-29 | 五河县金满塘生态种养殖家庭农场 | Fresh water live fish transportation management method |
| CN108887206A (en) * | 2018-06-15 | 2018-11-27 | 施乐谦 | A kind of freshwater fish waterless keep-alive transportation resources |
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Application publication date: 20110112 |