CN1660867A - Method of microeave radiation for preparing holoside in solid phase - Google Patents
Method of microeave radiation for preparing holoside in solid phase Download PDFInfo
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- CN1660867A CN1660867A CN 200410065805 CN200410065805A CN1660867A CN 1660867 A CN1660867 A CN 1660867A CN 200410065805 CN200410065805 CN 200410065805 CN 200410065805 A CN200410065805 A CN 200410065805A CN 1660867 A CN1660867 A CN 1660867A
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Abstract
A process for preparing oligose by solid-phase microwave radiation inclues condensation reaction between glucose or other monosaccharides or bisaccharides, water and catalyst under microwave radiation, baking, and pulverizing.
Description
Technical field
A kind of method of microeave radiation for preparing holoside in solid phase relates to the preparation method of oligose.
Background technology
Oligose is called oligosaccharides (oligosaccharide) again, refers generally to the glucosides that is made of 2-10 monose.Different oligosaccharides kinds and source have determined their distinctive biologic activity.Generally speaking, oligosaccharides has following several biological activity:
1, gi tract activity
Pure oligosaccharides has many important biological functions, can be used as a kind of short bifidobacterium growth factor as Polylevulosan, soybean oligosaccharide, isomaltose etc.Can improve body immunity, make pH value decline in the enteron aisle, suppress the harmful intestinal tract bacteria growing, produce vitamin B group, decompose carcinogenic substances promotes intestinal peristalsis and protein adsorption.Natural in addition pure oligosaccharides also has other as crucial functions such as energy supply, prevention decayed tooth, blood fat reducing and the absorptions of promotion mineral substance.
2, strengthen the hemopoietic function activity
Some oligosaccharides is the main effective constituent of Chinese medicine, and Chinese medicine has advantages such as pure natural, low side effect.The oligosaccharides effective constituent that extracted from Chinese medicine in recent years has a lot, and wherein the research of the oligose in Radix Glycyrrhizae and the Root of Medicinal Indian mulberry is especially noticeable.The glutinous rehmannia oligose can make CFU-S, CFU-GM, CFU-E and the BFU-E showed increased of rapid ageing model mice (SAMP8), and the WBC number that reduces in the peripheral blood is obviously increased.Point out it can stimulate the proliferation and differentiation of SAMP8 mouse hematopoietic stem cell, chief cell thus.The colony stimulating activity experiment shows that oligose can make the intravital G CFS of mouse produce showed increased.The bone marrow of mice result of study has confirmed further that also oligose can strengthen the effect of SAMP8 mouse hemopoietic function.Above-mentioned these experimental results disclose oligose can strengthen the hemopoietic function of SAMP8 mouse.
3, the preventing dental caries effect of oligosaccharides
Oligosaccharides such as Nutriflora P and the isomaltose of producing etc. can not had adhering insoluble glucan so do not generate by the glucosyl transferase cracking of bacterium, thereby reach the effect of preventing dental caries at present.
4, the stable blood sugar function of oligosaccharides
With the galactooligosaccharicomposition is example, the severe constipation patient is under the particular case of edible 30 gram oligosaccharides every day, blood glucose value does not have any variation, general health people every day, edible 3-5 restrained, blood glucose value can not change yet, this mainly is that oligosaccharides can not be broken down into monose in small intestine, so blood sugar concentration can not raise because do not decompose the enzyme of this oligosaccharides in the small intestine.
5, improve immunocompetence
Have the enhancing body immunity as oligochitosan, improving food configuration, improve food water retention property aspect and play an important role.In addition, oligochitosan has and can be made the index of the derivative of oligochitosan as medical diagnosis by the zymolytic character of bacteriolyze.Aspect medical, few six sugar of discovery N-acetyl shell such as Suzuki have very high anti-infective, antitumour activity in recent years; People such as Minami have verified that also oligochitosan has significant effect to the healing of wound, are expected to become practical medical products; Find that in addition oligochitosan also has signaling activity plant-growth, differentiation and organism aspect resisting.
The synthetic of the oligosaccharides of bibliographical information has enzyme process to synthesize and two kinds of methods of chemosynthesis.Yet enzyme process is synthetic to exist the easy inactivation of enzyme, the shared cost height of enzyme in the production, product separation and purification difficulty, problem such as productive rate is lower.Shortcomings such as the cost height is synthesized in the synthetic then existence of conventional chemical, and reaction intermediates prepares loaded down with trivial details, and output is little.
Summary of the invention
The method that the purpose of this invention is to provide a kind of microeave radiation for preparing holoside in solid phase, this is that a kind of reaction times is short, productive rate height, and environmental friendliness, the preparation method of the oligose of save energy.
Technical scheme of the present invention: under microwave irradiation effect, with water is reaction initiator, with monose, disaccharide, monose and monose or monose and disaccharide, with the initiator mixing, after carrying out condensation reaction in 3-10 minute under the katalysis of acid, cooling, pulverizing promptly obtain the oligose product.
Used glycogen material: monose is glucose, seminose, semi-lactosi, wood sugar, and disaccharide is sucrose, lactose.
In the aforesaid method, reaction initiator is selected water for use, and its add-on is counted the 5%-30% of raw material sugar with the quality percentage.Catalyzer is selected hydrochloric acid, sulfuric acid, citric acid or phosphoric acid for use, and its add-on is counted the 0.5%-3% of raw material sugar with the quality percentage.The Best Times of condensation reaction is 5-8 minute; Microwave frequency is 2450MHz, and power is 100-500W.
Beneficial effect of the present invention: microwave is the hertzian wave of a kind of frequency at 300MHz-300GHz.Microwave just is widely used in industry, agricultural, medical treatment and scientific research after being found and having heat effect.The maximum characteristics of microwave heating are to produce heat simultaneously at the different depths of heated material, and also this just " internal heating " make rate of heating fast and even, and about temperature is identical.The rapid heating of microwave has shortened the reaction times, has saved the energy.Just because this heat characteristic of microwave, make its can be directly and chemical system have an effect, thereby promote the carrying out of all kinds of chemical reactions.
Microwave technology is applied to organic synthesis, has the shortening reaction times, improve reaction yield, advantages of environment protection.Experiment shows that the present invention adopts microwave-assisted synthesis to prepare oligose, has productive rate height, reaction times weak point, environmental friendliness, advantage such as pollution-free.
Embodiment
Claim of the present invention is not limited to following examples.
Embodiment 1
Take by weighing glucose 20 grams, place the 200ml beaker, add initiator water 6 grams, hydrochloric acid 0.5 gram mixes.Place microwave organic synthesis instrument, regulate corresponding microwave power and between 100-500W, change, react after 5 minutes, be cooled to room temperature.Pulverize the oligose product, oligosaccharide contg accounts for about 80% in the product, if use the ethanol sedimentation method, purity can be brought up to more than 90%.
Embodiment 2
Take by weighing each 5 gram of glucose and seminose and mix, place the 200ml beaker, add initiator water 0.5 gram, sulfuric acid 0.3 gram mixes.Place microwave organic synthesis instrument, regulate corresponding microwave power and between 100-500W, change, react after 8 minutes, be cooled to room temperature, pulverize the oligose product, oligosaccharide contg accounts for about 70% in the product.
Embodiment 3
Take by weighing sucrose 20 grams, place the 200ml beaker, add initiator water 4 grams, phosphoric acid 0.4 gram mixes.Place microwave organic synthesis instrument, regulate corresponding microwave power and between 100-500W, change, react after 3 minutes, be cooled to room temperature.Pulverize behind the product dehydrated alcohol quiescent setting, throw out oven dry the oligose product, oligosaccharide contg accounts for about 60% in the product.
Embodiment 4
Take by weighing each 10 gram of lactose and wood sugar and mix, place the 200ml beaker, add initiator water 2 grams, citric acid 0.1 gram mixes.Place microwave organic synthesis instrument, regulate corresponding microwave power and between 100-500W, change, react after 10 minutes, be cooled to room temperature.Product precipitates with dehydrated alcohol, pulverize after the throw out oven dry the oligose product, oligosaccharide contg accounts for about 65% in the product.
Claims (7)
1, a kind of preparation method of oligose, it is characterized in that under microwave irradiation effect, with water is reaction initiator, with monose, disaccharide, monose and monose or monose and disaccharide, with the initiator mixing, under the katalysis of acid, carry out condensation reaction in 3-10 minute, cooling, pulverize, promptly obtain the oligose product.
2, the preparation method of oligose according to claim 1, monose wherein is glucose, seminose, semi-lactosi, wood sugar, disaccharide is sucrose, lactose.
3, the preparation method of oligose according to claim 1, wherein said microwave frequency are 2450MHz.
4, the preparation method of oligose according to claim 1, wherein said reaction initiator is a water; Catalysts is hydrochloric acid, sulfuric acid, citric acid or phosphoric acid.
5, the preparation method of oligose according to claim 1, wherein said condensation reaction time is 5-8 minute.
6, the preparation method of oligose according to claim 1, the add-on of wherein said catalyst acid count the 0.5%-3% of raw material sugar with the quality percentage.
7, the preparation method of oligose according to claim 1, the add-on of wherein said initiator water count the 5%-30% of raw material sugar with the quality percentage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410065805 CN1272336C (en) | 2004-12-15 | 2004-12-15 | Method of microeave radiation for preparing holoside in solid phase |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410065805 CN1272336C (en) | 2004-12-15 | 2004-12-15 | Method of microeave radiation for preparing holoside in solid phase |
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| CN1660867A true CN1660867A (en) | 2005-08-31 |
| CN1272336C CN1272336C (en) | 2006-08-30 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766695A (en) * | 2013-12-26 | 2014-05-07 | 江南大学 | Polydextrose with controllable molecular weight and rapid preparation method thereof |
| CN103833868A (en) * | 2013-12-26 | 2014-06-04 | 江南大学 | Rapid preparation method for megalosaccharide |
| CN105001349A (en) * | 2015-07-31 | 2015-10-28 | 江南大学 | Preparation method and application of cordyceps active oligosaccharide |
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2004
- 2004-12-15 CN CN 200410065805 patent/CN1272336C/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766695A (en) * | 2013-12-26 | 2014-05-07 | 江南大学 | Polydextrose with controllable molecular weight and rapid preparation method thereof |
| CN103833868A (en) * | 2013-12-26 | 2014-06-04 | 江南大学 | Rapid preparation method for megalosaccharide |
| CN103766695B (en) * | 2013-12-26 | 2015-05-20 | 江南大学 | Polydextrose with controllable molecular weight and rapid preparation method thereof |
| CN105001349A (en) * | 2015-07-31 | 2015-10-28 | 江南大学 | Preparation method and application of cordyceps active oligosaccharide |
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| Publication number | Publication date |
|---|---|
| CN1272336C (en) | 2006-08-30 |
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Address after: No. 1800 road 214122 Jiangsu Lihu Binhu District City of Wuxi Province Patentee after: Jiangnan University Address before: 214036 Jiangsu city of Wuxi Province River Road No. 170 Patentee before: Jiangnan University |