CN1623960A - General microbial fertilizer - Google Patents
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- CN1623960A CN1623960A CN 200410088851 CN200410088851A CN1623960A CN 1623960 A CN1623960 A CN 1623960A CN 200410088851 CN200410088851 CN 200410088851 CN 200410088851 A CN200410088851 A CN 200410088851A CN 1623960 A CN1623960 A CN 1623960A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 98
- 230000000813 microbial effect Effects 0.000 title claims abstract description 70
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 25
- 239000011574 phosphorus Substances 0.000 claims description 25
- 229910052698 phosphorus Inorganic materials 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 22
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 17
- 239000011591 potassium Substances 0.000 claims description 17
- 229910052700 potassium Inorganic materials 0.000 claims description 17
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- 244000005700 microbiome Species 0.000 claims description 13
- 229910019142 PO4 Inorganic materials 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 10
- 239000010452 phosphate Substances 0.000 claims description 10
- 241000195940 Bryophyta Species 0.000 claims description 7
- 239000003415 peat Substances 0.000 claims description 7
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 4
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 4
- 239000011609 ammonium molybdate Substances 0.000 claims description 4
- 229940010552 ammonium molybdate Drugs 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 229910021538 borax Inorganic materials 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- ZFXVRMSLJDYJCH-UHFFFAOYSA-N calcium magnesium Chemical compound [Mg].[Ca] ZFXVRMSLJDYJCH-UHFFFAOYSA-N 0.000 claims description 4
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 4
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 4
- 239000011790 ferrous sulphate Substances 0.000 claims description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
- 239000004328 sodium tetraborate Substances 0.000 claims description 4
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- 229960001763 zinc sulfate Drugs 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 22
- 230000000694 effects Effects 0.000 abstract description 22
- 230000035558 fertility Effects 0.000 abstract description 6
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 30
- 239000007788 liquid Substances 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 18
- 238000004519 manufacturing process Methods 0.000 description 15
- 239000000725 suspension Substances 0.000 description 14
- 239000000047 product Substances 0.000 description 12
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- 238000011161 development Methods 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 7
- 229930006000 Sucrose Natural products 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 239000005720 sucrose Substances 0.000 description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 6
- 241000726221 Gemma Species 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 6
- 108010080698 Peptones Proteins 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 6
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 6
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 6
- 229940095054 ammoniac Drugs 0.000 description 6
- 238000009792 diffusion process Methods 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
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- 235000011389 fruit/vegetable juice Nutrition 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 235000019319 peptone Nutrition 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 239000008107 starch Substances 0.000 description 6
- 235000019698 starch Nutrition 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000007423 decrease Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 235000012204 lemonade/lime carbonate Nutrition 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000005056 compaction Methods 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 238000005469 granulation Methods 0.000 description 3
- 230000003179 granulation Effects 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 238000000643 oven drying Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 241000193755 Bacillus cereus Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 241000237502 Ostreidae Species 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012262 fermentative production Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 235000020636 oyster Nutrition 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- WHBMMWSBFZVSSR-UHFFFAOYSA-N 3-hydroxybutyric acid Chemical class CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 244000106835 Bindesalat Species 0.000 description 1
- 235000000318 Bindesalat Nutrition 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000881860 Paenibacillus mucilaginosus Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000007633 bacillus mucilaginosus Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000003458 metachromatic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000009329 organic farming Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A universal microbial fertilizer features that new bacterial strains are used and its active components are regulated, resulting in high fertility and high effect to increase yield of agricultural crops.
Description
Technical field
Technical solutions according to the invention belong to the agricultural fertilizer field, concretely, are the microbial fertilizer fields that belongs in the biological fertilizer field, according to International Patent Classification, belong to CO5F 11/08 field.
Background technology
China has a large population, ploughs few, and along with successively decreasing year by year in the development arable land in population growth, industry and city.According to the statistics made by the departments concerned, China's population increases by 1,600 ten thousand every year on average, and 4,000,000 mu of the annual minimizings of ploughing.Nineteen fifty population 5.5 hundred million, plough 16.5 hundred million mu, 2.99 mu of per capita cultivated lands, the per capita cultivated land was 1.51 mu in 1980, the nineteen ninety per capita cultivated land drops to 1.1 mu, is significantly less than 4.03 mu of world per capita cultivated lands' level, also is lower than 2.1 mu of Asia per capita cultivated lands' level.For this reason, unique feasible way of China's increase agricultural output is to increase the yield per unit area.Could satisfy the demand of industrial development and population increase to agricultural-food, chemical fertilizer is also with regard to the natural widespread use that obtained.
The soil compaction that unreasonable for a long time use chemical fertilizer brings, soil fertility decline, ecological damage, environmental pollution and agricultural byproducts quality decline problem have been subjected to the great attention of national departments concerned and agricultural science and technology circle.Using in a large number of chemical fertilizer caused soil fertility decline, the disadvantage that soil compaction and agriculture production cost improve constantly.In recent decades, China makes great efforts to explore the rational application of fertilizer always, overcomes the drawback of chemical fertilizer and the effective way of raising chemical fertilizer utilization ratio.
Agricultural fertilizer comprises chemical fertilizer and bio-feritlizer, is the irreplaceable important production means of China's agriculture production.Wherein, bio-feritlizer is as the important branch of agro-biological engineering subject and the important component part of soil fertilizer science, develops rapidly all having obtained aspect fundamental research and the applied research in nearly ten years.And the microbial fertilizer in the agricultural fertilizer (belonging to a kind of of bio-feritlizer) more and more is familiar with by people as a kind of important effect of agrotechnical measure in the development an agriculture featuring high yields, fine quality and high efficiency, this provides sizable development space for its exploitation, popularization, application, particularly the irreplaceability in development Organic farming, production A level, AA level green food has demonstrated fully its potential glamour.
China's microbial fertilizer development in recent years is very fast, has many enterprises to be engaged in production and operation, and kind constantly increases, and also has some external products to squeeze into the domestic market simultaneously.But for various reasons, the quality product of existing market is uneven, has occurred some problems in the production application.According to Ministry of Agriculture's random checking recent years, tracking selective examination, the situation of country's system inspection is seen, spot-check qualification rate less than 60%, and product quality problem mainly is that effective bacterium number is not up to standard, and the quality guaranteed period is too short.Living bacteria count is the important indicator of quality product in the microbial fertilizer, and living bacteria count is not up to standard, and quality product just can not get guaranteeing that what reflect is the effect instability.But, the bacterial classification reason is not the sole cause that influences effect of fertilizer, in fact, composition proportion in the fertilizer is also affecting the effect of fertilizer greatly, if can be,, coordinate the relation between bacterial classification and the composition proportion and obtain better effect by the innovation of fertilizer component proportioning solving the bacterial classification problem simultaneously, effectively improve output, can play great innovation effect the development in microbial fertilizer field.Under these circumstances, technical scheme provided by the invention can very significantly improve the effectiveness of microbial fertilizer by using new bacterial classification and the composition proportion in the fertilizer being innovated, and this makes the present invention seem very important and meaningful.
Summary of the invention
Microbial fertilizer is a kind of goods that cause obtaining the specific fertilizer effect with the effect of microbial life active, is a kind of of the fertilizer that uses in the agriculture production, and is different on main points and intension with traditional fertilizer and fertilizer.Microbial fertilizer is mixed by microbial strains agent and microbe fertilizer formulation and makes.The microbial fertilizer production cost is low, and is easy to use, and effect is good, and is free from environmental pollution, not only volume increase after using, and can improve agricultural product quality and reduce fertilizer amount.Microbial fertilizer is a fertilizer of producing green food, is the fertilizer of protection green environment, is rising fertilizer.Present this project China again is in new developmental stage.
The technical problem that solves
After soil compaction, soil fertility decline, ecological damage, environmental pollution and agricultural byproducts quality decline problem that national departments concerned is fully paid attention to using chemical fertilizer to bring, the microbial fertilizer in the bio-feritlizer has obtained swift and violent development.But owing to reasons such as bacterial classification, composition proportion, the quality product of existing market is uneven, has occurred some problems in production application.According to Ministry of Agriculture's random checking recent years, tracking selective examination, the situation of country's system inspection is seen, spot-check qualification rate less than 60%, and product quality problem mainly is that effective bacterium number is not up to standard, and the quality guaranteed period is too short.Living bacteria count is the important indicator of quality product in the microbial fertilizer, and living bacteria count is not up to standard, and quality product just can not get guaranteeing that what reflect is the effect instability that wherein reason mainly is the bacterial classification problem.But, the bacterial classification reason is not the sole cause that influences effect of fertilizer, in fact, other compositions in the fertilizer beyond the microbial strains and mutually proportion relation also affecting the effect of fertilizer greatly, because there is the relation of cooperation, antagonism mutually between each composition, appropriate ingredients, proportioning all have close influence for the growth of the microbial reproduction in the microbial fertilizer, activity and crop.If can by the innovation of fertilizer component and proportioning (also being prescription), and obtain better effect solving the bacterial classification problem simultaneously, effectively improve output, also can play great innovation effect to the development in microbial fertilizer field.Under these circumstances, technical scheme provided by the invention is not only by using novel bacterial to solve the bacterial classification problem, and the composition proportion in the fertilizer innovated, the microbial fertilizer of production has the effect of increasing production of highly significant, and this makes the present invention seem very important and meaningful.
Technical scheme
At first, it should be explicitly made clear at this point that per-cent and the ratio mentioned among the present invention all are meant weight percent and part by weight.
The invention provides a kind of microorganism (bacillusmusilaginosiengineering, Bacillus mucilaginosus), concrete, bacterial classification be named as No. 1, potassium " new " (on April 15th, 2004 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, preserving number is CGMCC1131), this microorganism separates from high-yielding grain fields, and through domestication, proterties is stable for a long time.The biological characteristics of this bacterial classification is that thalline is shaft-like, big or small 1-1.2*2-5 micron, Gram-positive, cell has metachromatic granules and beta-hydroxy-butanoic acid salt, and living or inferior end is given birth to ellipse in the gemma, do not make volumination, shaft-like thalline has chaining trend, the stability decision colonial morphology of chain, bacterium colony is an oyster white on the beef peptone substratum, and majority is circular, and the surface is slightly coarse, the edge is irregular, tarnish, non-pigment, diameter 2-6 millimeter.Can utilize multiple nitrogenous source, carbon source, 37 ℃ of optimal pHs 7.1 of growth temperature 20-55 ℃ of optimum growth temp.Under bad condition, thalline is converted into gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature and drying to the poor environment strong stress resistance.
The present invention also provides a kind of microorganism (bacillus cereus, Bacillus cereus), concrete, bacterial classification be named as No. 1, phosphorus " new " (on April 15th, 2004 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, preserving number is CGMCC1132), this microorganism separates from high-yielding grain fields, and through domestication, proterties is stable for a long time.The biological characteristics of this bacterial classification is that thalline is shaft-like, size 0.6-0.8*2-3 micron, Gram-positive, cellular plasm even dyeing, the flagellum adnation, give birth in the gemma or middle partially giving birth to, shape cylindricality or ellipse do not make volumination, bacterium colony is an oyster white on the beef peptone substratum, circle, edge irregular hair shape is outstanding, diameter 1-2 millimeter.Can utilize multiple nitrogenous source, carbon source, 37 ℃ of optimal pHs 7.1 of growth temperature 20-55 ℃ of optimum growth temp.Under bad condition, thalline is converted into gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature and drying to the poor environment strong stress resistance.
The present invention also provides a kind of prescription that uses when producing microbial fertilizer, called after " general microbial fertilizer formula ".
Concrete, the mode of production of this microbial fertilizer is as follows:
Earlier respectively to No. 1, new potassium and new No. 1 bacterial classification of phosphorus ferments and further (mix or respectively) be processed as the microbial strains agent, this inoculum agent is made microbial fertilizer according to " general microbial fertilizer formula " provided by the invention.
The applicant thinks that the bacterial classification of this general microbial fertilizer, the composition of other compositions and proportion relation have formed technical solutions according to the invention on the whole.Because the bacterial classification that this fertilizer uses and the composition of other compositions and proportioning and all existing fertilizer are different, so, this technical scheme has novelty, if the commercially available microbial fertilizer that this microbial fertilizer is common relatively, can increase substantially output, just have outstanding progress and outstanding feature, just had creativeness and practicality, had and be awarded Patent right primary condition.
New No. 1 bacterial classification of potassium in streak culture mode at new 1 substratum (new 1 substratum: sucrose 5g/L, sal epsom 0.5g/L, lime carbonate 0.1g/L, sodium-chlor 1.0g/L, iron trichloride 0.005g/L, Sodium phosphate dibasic 2g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8.) going up preservation, the short term storage condition is 4 ℃, activates per February 1 time; Long-term storage conditions is-20 ℃, activation in per 6 months 1 time.Training method is for after preserving the inclined-plane and getting kind, and line inserts slant medium (new 1 substratum), and 30-38 ℃ of incubator left standstill dark condition under cultivation 36 hours; Use the aseptic water washing inclined-plane, form bacteria suspension; 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium (new 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8) of about 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude; The bacteria suspension that obtains is joined (new 2 substratum: starch 10g/L in new 2 substratum of about 5-10 times volume, sucrose 10g/L, sal epsom 1g/L, sulfate of ammoniac 2g/L, Yeast diffusion juice 0.2g/L, lime carbonate 3g/L, sodium-chlor 1g/L, potassium primary phosphate 1g/L, PH transfers to 6.5-7.8), it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude; The bacteria suspension that obtains is joined (new 3 substratum: starch 10g/L in new 3 substratum of about 50-100 times volume, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.2g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1.5g/L, lime carbonate 1g/L, PH transfers to 6.5-7.8), it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude; The bacteria suspension that obtains is joined (new 4 substratum: starch 7g/L in new 4 substratum of about 5-10 times volume, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.1g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1g/L, lime carbonate 1g/L, PH transfers to 6.5-7.8), it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude.
New No. 1 bacterial classification of phosphorus in streak culture mode at new No. 1 substratum of phosphorus (new No. 1 substratum of phosphorus: peptone 5g/L, sodium-chlor 5g/L, extractum carnis 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8) go up preservation, the short term storage condition is 4 ℃, activates per February 1 time; Long-term storage conditions is-20 ℃, activation in per 6 months 1 time.Training method is for after preserving the inclined-plane and getting kind, and line inserts slant medium (new No. 1 substratum of phosphorus), and 30-38 ℃ of incubator left standstill dark condition under cultivation 36 hours; Use the aseptic water washing inclined-plane, form bacteria suspension; 1 volume bacteria suspension is inserted in No. 1 liquid nutrient medium of new phosphorus (new No. 1 liquid nutrient medium of phosphorus: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8) of about 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude; The bacteria suspension that obtains is joined No. 2 substratum of new phosphorus (new No. 2 substratum of phosphorus: starch 5g/L of about 5-10 times volume, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, extractum carnis 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8) in, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude; The bacteria suspension that obtains is joined No. 3 substratum of new phosphorus (new No. 3 substratum of phosphorus: starch 10g/L of about 100-200 times volume, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8) in, it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude; The bacteria suspension that obtains is joined No. 4 substratum of new phosphorus (new No. 4 substratum of phosphorus: starch 10g/L of about 20-40 times volume, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8) in, it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude.
Final fermented liquid balanced mix with No. 1, the final fermented liquid of No. 1, new potassium and new phosphorus; use high-quality peat composed of rotten mosses powder (commercially available again; carry out the dry sterilization postcooling) join in the blended fermented liquid according to the content of wanting to obtain microorganism in the microbial strains agent; fully stir; use the tablets press granulation; oven drying at low temperature; becoming microbial strains agent product [also can use high-quality peat composed of rotten mosses powder (commercially available respectively the final fermented liquid of No. 1, the final fermented liquid of No. 1, new potassium and new phosphorus; carry out the dry sterilization postcooling) join in the blended fermented liquid according to the content of wanting to obtain microorganism in the microbial strains agent; fully stir; use the tablets press granulation; oven drying at low temperature; become two kinds of microbial strains agent products, two kinds of different microbial strains agent are mixed use again.This inoculum agent and prescription provided by the invention are mixed, obtain general microbial fertilizer.Microbe fertilizer formulation provided by the invention (being the per-cent that its composition and proportioning account in final fertilizer) is specific as follows: ammonium sulfate 20-30%, fused(calcium magnesium)phosphate 1-3%, general calcium 15-20%, Repone K 7-13%, zinc sulfate 0.3-1%, ferrous sulfate 0.2-0.6%, borax 0.1-0.3%, ammonium molybdate 0-0.3%, copper sulfate 0.3-0.7%, manganous sulfate 0.1-0.4%, peat composed of rotten mosses 30-50%, the microbial strains agent accounts for 3-15%.
Useful effect
The microbial fertilizer of producing according to technical scheme provided by the invention has higher fertilizer efficiency and long quality guaranteed period, has solved the short and bad problem of fertilizer efficiency of quality guaranteed period.
Embodiment
Embodiment 1: new No. 1 fermentative production of potassium
Earlier No. 1 bacterial classification of new potassium is activated, use 5ml aseptic water washing inclined-plane again, form bacteria suspension, bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours, culture condition is 37 ℃, 180-200rpm detects cell concentration, reaches 108 orders of magnitude in concentration; 100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 10 in concentration
8The order of magnitude; Bacterium liquid in the 2000ml triangular flask is inserted 1m
3In the fermentor tank, the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m
3/ h cultivated 8 hours; Detect cell concentration, reach 10 in concentration
8The order of magnitude; With 1m
3Bacterium liquid in the fermentor tank inserts 5m
3Fermentor tank, access amount are 500 liters, and this fermentor tank contains 3m
3New 4 substratum, fermentation condition is: temperature 36-38 ℃, tank pressure 0.5kg/cm, air flow 120m
3/ h cultivated 15 hours.Detect cell concentration, reach 10 in concentration
9The order of magnitude stops fermentation.
Embodiment 2: the fermentative production that No. 1, new phosphorus
Earlier No. 1 bacterial classification of new phosphorus is activated, use 5ml aseptic water washing inclined-plane again, form bacteria suspension, bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains No. 1 liquid nutrient medium of the new phosphorus of 100ml, uses shaking table to cultivate 18 hours, culture condition is 37 ℃, 180-200rpm detects cell concentration, reaches 10 in concentration
8The order of magnitude carries out next step; 100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains No. 2 substratum of the new phosphorus of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 10 in concentration
8The order of magnitude carries out next step; Insert 1m with taking out bacterium liquid in the 2000ml triangular flask
3In the fermentor tank, the access amount is 3.5 liters, contains No. 3 substratum of the new phosphorus of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m
3/ h cultivated 8 hours; Detect cell concentration, reach 10 in concentration
8The order of magnitude descends to relate to; With 1m
3Bacterium liquid in the fermentor tank takes out and inserts 5m
3Fermentor tank, access amount are 130 liters, and this fermentor tank contains 3m
3New 4 substratum, fermentation condition is: temperature 36-38 ℃, tank pressure 0.5kg/cm, air flow 120m
3/ h cultivated 15 hours.Detect cell concentration, reach 10 in concentration
9The order of magnitude stops fermentation.
Embodiment 3: the production of inoculum agent
No. 1 fermented liquid balanced mix of new phosphorus that No. 1 fermented liquid of new potassium that embodiment 1 is made and embodiment 2 make, mix with the high-quality peat composed of rotten mosses powder (commercially available) that carries out the dry sterilization postcooling in 1: 4 ratio then, carry out granulation again, oven drying at low temperature becomes the microbial strains agent.
Embodiment 4: the preparation of microbial fertilizer
The microbial strains agent that embodiment 3 is made further is processed as microbial fertilizer according to prescription provided by the invention.Concrete, in the microbial fertilizer of finally making, ammonium sulfate 28%, fused(calcium magnesium)phosphate 2%, general calcium 16%, Repone K 10%, zinc sulfate 0.5%, ferrous sulfate 0.4%, borax 0.2%, ammonium molybdate 0.2%, copper sulfate 0.5%, manganous sulfate 0.2%, the peat composed of rotten mosses 30%, microbial strains agent 12%.
Embodiment 5: the fertilizer efficiency contrast of microbial fertilizer
Microbial fertilizer and commercially available microbial fertilizer that the fertilizer efficiency contrast of microbial fertilizer has used the present invention to make.Fertilizer and the commercially available microbial fertilizer produced by embodiment 1,2,3,4 methods carry out effect comparison respectively under following field experiment condition, wherein preserved 2 years in the fertilizer storehouse in position of embodiment 1,2,3,4 methods production.
On Different Crop, carry out the fertilizer efficiency experiment (blank is set) of microbial fertilizer provided by the invention and commercially available microbial fertilizer, 0.16 mu of each experiment sub-district area, soil is the loamy texture moisture soil, and physical features is smooth, and fertility is even, 0-20cm soil layer result of laboratory test is: the soil organism 1.21%, full nitrogen 0.071%, alkali-hydrolyzable nitrogen 54ppm, rapid available phosphorus 13ppm, available potassium 113ppm, 3 repetitions are established in experiment, execute 25 kilograms of microbial fertilizers for every mu during experiment, all as base manure, once executed, do not appended.Blank does not apply fertilizer.
Experimental result following (data are the volume increase per-cent of each fertilizer with respect to blank)
| Tomato | Peanut | Romaine lettuce | Wheat | Corn | Paddy rice | Green pepper | Cucumber | |
| Commercially available microbial fertilizer | 14 | ?10 | ?10 | ?15 | ?13 | ?15 | ?14 | ?35 |
| Microbial fertilizer provided by the invention | 27 | ?21 | ?31 | ?29 | ?19 | ?21 | ?30 | ?48 |
Embodiment 6: the microbial fertilizer that utilizes method preparation provided by the invention is carried out the bacterium number detect under long-time stock's condition
According to the viable bacteria content in the dilution plate number scale detection microbial fertilizer of routine.Take by weighing microbial fertilizer 1 gram (stock 2 years), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of LB substratum (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial fertilizer dilution with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is every gram microbial fertilizer.We carry out the detection of bacterium number to the microbial fertilizer that utilizes method preparation provided by the invention under long-time stock's (2 years) condition, detected result is 0.62*10
8Individual/gram, (national standard is 0.2*10 for 3.1 times of national standard
8Individual/gram).Solved microbial fertilizer kind microbe number problem and the too short problem of preservation term not up to standard.
The result shows, this microbial fertilizer can finely play a role, the bacterium number is up to standard after the prolonged preservation, the fertility effect is fine, has good effect of increasing production, and microbial fertilizer provided by the invention is compared with commercially available microbial fertilizer, bigger volume increase ability is arranged, having significant effect, is obvious improvement, has solved issue to be resolved of the present invention.
Claims (3)
1. microbial fertilizer, it is characterized in that it contains No. 1 microorganism of new potassium provided by the invention and new No. 1 microorganism of phosphorus simultaneously, the new preserving number of No. 1 microorganism of potassium at China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC1131, and new No. 1 preserving number at China Committee for Culture Collection of Microorganisms common micro-organisms center of phosphorus is CGMCC1132.
2. the described microbial fertilizer of claim 1, the composition and the proportioning (weight percent) specific as follows that it is characterized in that it: ammonium sulfate 20-30%, fused(calcium magnesium)phosphate 1-3%, general calcium 15-20%, Repone K 7-13%, zinc sulfate 0.3-1%, ferrous sulfate 0.2-0.6%, borax 0.1-0.3%, ammonium molybdate 0-0.3%, copper sulfate 0.3-0.7%, manganous sulfate 0.1-0.4%, peat composed of rotten mosses 30-50%, the microbial strains agent accounts for 3-15%.
3. the described microbial fertilizer of claim 2, the composition and the proportioning (weight percent) specific as follows that it is characterized in that it: ammonium sulfate 28%, fused(calcium magnesium)phosphate 2%, general calcium 16%, Repone K 10%, zinc sulfate 0.5%, ferrous sulfate 0.4%, borax 0.2%, ammonium molybdate 0.2%, copper sulfate 0.5%, manganous sulfate 0.2%, the peat composed of rotten mosses 30%, microbial strains agent 12%.
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| CN 200410088851 CN1250487C (en) | 2004-11-08 | 2004-11-08 | General microbial fertilizer |
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| CN 200410088851 CN1250487C (en) | 2004-11-08 | 2004-11-08 | General microbial fertilizer |
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| CN1250487C CN1250487C (en) | 2006-04-12 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399711A (en) * | 2011-09-05 | 2012-04-04 | 麻林涛 | Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof |
| CN102432362A (en) * | 2011-09-05 | 2012-05-02 | 麻林涛 | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof |
-
2004
- 2004-11-08 CN CN 200410088851 patent/CN1250487C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399711A (en) * | 2011-09-05 | 2012-04-04 | 麻林涛 | Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof |
| CN102432362A (en) * | 2011-09-05 | 2012-05-02 | 麻林涛 | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof |
| CN102399711B (en) * | 2011-09-05 | 2013-08-14 | 麻林涛 | Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof |
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