CN1257267C - Potassium bacteria and process for producing microorganism manure strain agent using said bacteria - Google Patents
Potassium bacteria and process for producing microorganism manure strain agent using said bacteria Download PDFInfo
- Publication number
- CN1257267C CN1257267C CN 200410088855 CN200410088855A CN1257267C CN 1257267 C CN1257267 C CN 1257267C CN 200410088855 CN200410088855 CN 200410088855 CN 200410088855 A CN200410088855 A CN 200410088855A CN 1257267 C CN1257267 C CN 1257267C
- Authority
- CN
- China
- Prior art keywords
- new
- substratum
- sucrose
- potassium
- fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Fertilizers (AREA)
Abstract
The technical scheme provided by the present invention belongs to a microorganism manure field of an organism manure field. According to conditions of national united inspection, the sample qualified rate of microorganism manure is lower than 60%, product quality has the main problems that effective bacterium amount does not reach the standard and guarantee periods are too short, and surviving capability is decreased and surviving time is short in a preserving process after strain is prepared into microorganism strain agents. Therefore, in the process of production, the problems are represented in that strain activity is decreased, production performance is weakened, propagation speed is slow and stress resistance is poor. The new strain provided by the present invention and the microorganism strain agents produced by the new technical scheme can better solve the problems that the strain amount does not reach the standard and the guarantee periods are not extended, and the present invention is very important and significant.
Description
Technical field
Technical solutions according to the invention belong to the agricultural fertilizer field, concretely, are the microbial fertilizer fields that belongs in the biological fertilizer field, according to International Patent Classification, belong to C05F 11/08 field.
Background technology
China has a large population, ploughs few, and along with successively decreasing year by year in the development arable land in population growth, industry and city.According to the statistics made by the departments concerned, China's population increases by 1,600 ten thousand every year on average, and 4,000,000 mu of the annual minimizings of ploughing.Nineteen fifty population 5.5 hundred million, plough 16.5 hundred million mu, 2.99 mu of per capita cultivated lands, the per capita cultivated land was 1.51 mu in 1980, the nineteen ninety per capita cultivated land drops to 1.1 mu, is significantly less than 4.03 mu of world per capita cultivated lands' level, also is lower than 2.1 mu of Asia per capita cultivated lands' level.For this reason, unique feasible way of China's increase agricultural output is to increase the yield per unit area.Could satisfy the demand of industrial development and population increase to agricultural-food, chemical fertilizer is also with regard to the natural widespread use that obtained.
The soil compaction that unreasonable for a long time use chemical fertilizer brings, soil fertility decline, ecological damage, environmental pollution and agricultural byproducts quality decline problem have been subjected to the great attention of national departments concerned and agricultural science and technology circle.Using in a large number of chemical fertilizer caused soil fertility decline, the disadvantage that soil compaction and agriculture production cost improve constantly.In recent decades, China makes great efforts to explore the rational application of fertilizer always, overcomes the drawback of chemical fertilizer and the effective way of raising chemical fertilizer utilization ratio.
Agricultural fertilizer comprises chemical fertilizer and bio-feritlizer, is the irreplaceable important production means of China's agriculture production.Wherein, bio-feritlizer is as the important branch of agro-biological engineering subject and the important component part of soil fertilizer science, develops rapidly all having obtained aspect fundamental research and the applied research in nearly ten years.And the microbial fertilizer in the agricultural fertilizer (belonging to a kind of of bio-feritlizer) more and more is familiar with by people as a kind of important effect of agrotechnical measure in the development an agriculture featuring high yields, fine quality and high efficiency, this provides sizable development space for its exploitation, popularization, application, particularly the irreplaceability in development Organic farming, production A level, AA level green food has demonstrated fully its potential glamour.
China's microbial fertilizer development in recent years is very fast, has many enterprises to be engaged in production and operation, and kind constantly increases, and also has some external products to squeeze into the domestic market simultaneously.Owing to reasons such as bacterial classifications, the quality product of existing market is uneven, has occurred some problems in the production application.According to Ministry of Agriculture's random checking recent years, tracking selective examination, the situation of country's system inspection is seen, spot-check qualification rate less than 60%, and product quality problem mainly is that effective bacterium number is not up to standard, and the quality guaranteed period is too short.Living bacteria count is the important indicator of quality product in the microbial fertilizer, living bacteria count is not up to standard, quality product just can not get guaranteeing, what reflect is the effect instability, wherein reason mainly is the bacterial classification problem, bacterial classification is after being prepared as the microbial strains agent, and the survival ability in preserving process descends, and the survival time shortens.Show as bacterial activity and descend in the production use, production performance weakens, and reproduction speed is slack-off, and resistance is poor.Therefore, the bacterial classification problem perplexs the production and the development of China's microbial fertilizer always.Under these circumstances, bacterial classification provided by the invention and the microbial strains agent that utilizes this bacterial classification to produce can extraordinaryly solve the problem that the bacterium number is up to standard and the quality guaranteed period prolongs, and this makes the present invention seem very important and meaningful.
Summary of the invention
Microbial fertilizer is a kind of goods that cause obtaining the specific fertilizer effect with the effect of microbial life active, is a kind of of the fertilizer that uses in the agriculture production, and is different on main points and intension with traditional fertilizer and fertilizer.The microbial fertilizer production cost is low, and is easy to use, and effect is good, and is free from environmental pollution, not only volume increase after using, and can improve agricultural product quality and reduce the traditional fertilizer consumption.Microbial fertilizer is a fertilizer of producing green food, is the fertilizer of protection green environment, is rising fertilizer.Present this project China again is in new developmental stage.
The technical problem that solves
After soil compaction, soil fertility decline, ecological damage, environmental pollution and agricultural byproducts quality decline problem that national departments concerned is fully paid attention to using chemical fertilizer to bring, microbial fertilizer in the bio-feritlizer has obtained swift and violent development, but, owing to reasons such as bacterial classifications, the quality product of existing market is uneven, has occurred some problems in production application.According to Ministry of Agriculture's random checking recent years, tracking selective examination, the situation of country's system inspection is seen, spot-check qualification rate less than 60%, and product quality problem mainly is that effective bacterium number is not up to standard, and the quality guaranteed period is too short.Living bacteria count is the important indicator of quality product in the microbial fertilizer, living bacteria count is not up to standard, quality product just can not get guaranteeing, what reflect is that effect instability, quality guaranteed period are short, wherein reason mainly is the bacterial classification problem, bacterial classification is after being prepared as the microbial strains agent, and the survival ability in preserving process descends, and the survival time shortens.This problem is actually owing to the employed bacterial classification of these microbial strains agent or can not produces gemma, the ability that produces gemma is more weak, perhaps the gemma that produces is sprouted ability, can not finely spend stress conditions in the microbial strains agent production process and stock, preserving process, thereby showing as bacterial activity in the production use descends, production performance weakens, and reproduction speed is slack-off or the like.The bacterial classification problem perplexs the production and the development of China's microbial fertilizer always.Under these circumstances, novel bacterial provided by the invention and the microbial strains agent that utilizes new solution to produce can extraordinaryly solve the problem that the bacterium number is up to standard and the quality guaranteed period prolongs, and this makes the present invention seem very important and meaningful.
Technical scheme
At first, it should be explicitly made clear at this point that per-cent and the ratio mentioned among the present invention all are meant weight percent and part by weight.
The invention provides a kind of technical scheme that addresses the above problem, concrete, the invention provides a kind of microorganism (bacillusmusilaginosiengineering, Bacillus mucilaginosus), concrete, bacterial classification is named as " No. 1, new potassium ", and (it was in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on April 15th, 2004, preserving number is CGMCC1131), this microorganism separates from high-yielding grain fields, and through domestication, proterties is stable for a long time.
The biological characteristics of this bacterial classification is that thalline is shaft-like, big or small 1-1.2*2-5 micron, Gram-positive, cell has metachromatic granules and beta-hydroxy-butanoic acid salt, and living or inferior end is given birth to ellipse in the gemma, do not make volumination, shaft-like thalline has chaining trend, the stability decision colonial morphology of chain, bacterium colony is an oyster white on the beef peptone substratum, and majority is circular, and the surface is slightly coarse, the edge is irregular, tarnish, non-pigment, diameter 2-6 millimeter.Can utilize multiple nitrogenous source, carbon source, growth temperature 20-55 ℃, 37 ℃ of optimum growth temps, optimal pH 7.1.Under bad condition, thalline transforms and produces gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature and drying to the poor environment strong stress resistance.
The applicant thinks, because bacterial classification is new, so the technical scheme that this bacterial classification uses traditional microbial strains agent and process for producing same to produce the microbial strains agent has novelty, (effectively the bacterium number is not up to standard if the microbial strains agent of producing can overcome above-mentioned shortcoming, quality guaranteed period is too short), have outstanding characteristics and obvious improvement, just had creativeness and practicality, had and be awarded Patent right primary condition.
Substratum that traditional microbial strains agent is used in producing and cultural method have detailed description in " potassium bacterium " volumes such as (, agriculture press, nineteen fifty-nine, Beijing) Chen Yanwei book.
Simultaneously, the applicant gropes the fermentation condition of No. 1, new potassium in the present invention, has drawn 1 and has been highly suitable for this culture of strains base and fermentation condition (this substratum and fermentation condition also are applicable to other part bacterial classifications).The applicant thinks, because bacterial classification is new, substratum provided by the invention and fermentation condition also are new, so the technical scheme that this bacterial classification uses new microbial fermentation condition provided by the invention to produce the microbial strains agent has novelty, if the microbial strains agent of producing can overcome above-mentioned shortcoming (effectively the bacterium number is not up to standard, and the quality guaranteed period is too short), have outstanding characteristics and obvious improvement, just had creativeness and practicality, had and be awarded Patent right primary condition.
This new substratum that culture scheme used has:
New 1 substratum (slant medium): sucrose 5g/L, sal epsom 0.5g/L, lime carbonate 0.1g/L, sodium-chlor 1.0g/L, iron trichloride 0.005g/L, Sodium phosphate dibasic 2g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8.
New 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8.
New 2 substratum: starch 10g/L, sucrose 10g/L, sal epsom 1g/L, sulfate of ammoniac 2g/L, Yeast diffusion juice 0.2g/L, lime carbonate 3g/L, sodium-chlor 1g/L, potassium primary phosphate 1g/L, PH transfers to 6.5-7.8.
New 3 substratum: starch 10g/L, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.2g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1.5g/L, lime carbonate 1g/L, PH transfers to 6.5-7.8.
New 4 substratum: starch 7g/L, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.1g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1g/L, lime carbonate 1g/L, PH transfers to 6.5-7.8.
More than all substratum all before use through high pressure steam sterilization (121 ℃, 1.1kg/cm
3, 30 minutes), cooling.
The concrete mode of production of microbial strains agent:
One, culture presevation and activation culture
Bacterial classification is gone up preservation in streak culture mode at new 1 substratum (slant medium), and the short term storage condition is 4 ℃, activates per February 1 time; Long-term storage conditions is-20 ℃, activation in per 6 months 1 time.
Two, microorganism culturing
1, microorganism culturing (traditional microbial fermentation production method)
Substratum: starch 10%, soybean cake powder 10%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.1%, sal epsom 0.05%, iron(ic) chloride 0.001%, lime carbonate 0.01%, yeast extract paste 0.01%, PH7.2-7.4.
To insert behind the actication of culture in the above-mentioned substratum of 1000ml, triangular flask shakes bottle 160RPM30-38 ℃ and cultivated 16 hours, be inoculated in the 200L seed fermentation jar by 1% inoculum size then and (still be substratum of the same race), ventilation, cultivated 16 hours for 30-38 ℃, receive (still being substratum of the same race) in the 2000L fermentor tank by 10% inoculum size again, cultivate for 30-38 ℃ and be warming up to 40-45 ℃ after 20 hours, treat to stop fermentation after 80% thalline produces gemma cultivation 10 hours.
2, microorganism culturing (method provided by the invention):
1) preserve the inclined-plane certainly and get kind after, line inserts slant medium (new 1 substratum), 30-38 ℃ of incubator left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of about 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of about 5-10 times volume, and it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of about 50-100 times volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of about 5-10 times volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude.
Three, the production of microbial strains agent
High-quality peat composed of rotten mosses powder (commercially available) is carried out the dry sterilization postcooling; join in the fermented liquid that fermentation ends (traditional microbial fermentation production method or production method provided by the invention) obtains according to the content of wanting to obtain microorganism in the microbial strains agent; fully stir; use the tablets press granulation; oven drying at low temperature; be packaged into Kucheng and be microbial strains agent product, mixing with common chemical fertilizer or organic carrier as required during use to become microbial fertilizer.
So, can utilize potassium bacterium provided by the invention, use known microbial fermentation processes to ferment and further be processed into the microbial strains agent.And can be with the potassium bacterial micro-organism inoculum agent made through further processing, with known chemical fertilizer and or organic carrier mix microbial fertilizer.
Useful effect
Use the microbial strains agent of bacterial classification production provided by the present invention that higher effective bacterium number and long quality guaranteed period are arranged.
Use the traditional zymotic mode to carry out the inoculum agent of fermentative production at this bacterial classification, carry out effective bacterium number during warehouse-in and detect, its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effective bacterium number is 3.3 times of national standard.Solved strain number problem not up to standard.
The inoculum agent warehouse-in that uses fermentation mode provided by the invention to carry out fermentative production at this bacterial classification the time carries out effective bacterium number and detects, and its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effectively the bacterium number is 4 times of national standard.Also solved strain number problem not up to standard.
The inoculum agent that this bacterial classification uses the traditional zymotic mode to carry out fermentative production is preserved 2 years in the warehouse after, carry out effective bacterium number and detect, its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effective bacterium number is 3 times of national standard.Solved short problem of quality guaranteed period.
The inoculum agent that this bacterial classification uses fermentation mode provided by the invention to carry out fermentative production is preserved 2 years in the warehouse after, carry out effective bacterium number and detect, its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effective bacterium number is 3.6 times of national standard.Solved short problem of quality guaranteed period.
Embodiment
Embodiment 1: utilize traditional method that new potassium is carried out the agent of fermentative preparation microbial strains for No. 1
Substratum: starch 0.5%, sucrose 0.1%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.1%, sal epsom 0.05%, iron(ic) chloride 0.001%, lime carbonate 0.01%, yeast extract paste 0.02%, PH7.2-7.4.
To insert behind the actication of culture in the above-mentioned substratum of 1000ml, triangular flask shakes bottle 160RPM and cultivated 16 hours for 37 ℃, be inoculated in the 200L seed fermentation jar by 1% inoculum size then and (still be substratum of the same race), ventilation, cultivated 16 hours for 37 ℃, receive (still being substratum of the same race) in the 2000L fermentor tank by 10% inoculum size again, cultivate for 37 ℃ and be warming up to 42 ℃ after 20 hours, treat to stop fermentation after 80% thalline produces gemma cultivation 10 hours.Detect cell concentration, reach 10
9, mix with the high-quality peat composed of rotten mosses powder (commercially available) that carries out the dry sterilization postcooling by 1: 4 weight percent then, carry out granulation again, oven drying at low temperature becomes the microbial strains agent.
Embodiment 2: utilize method provided by the invention that new potassium is carried out the agent of fermentative preparation microbial strains for No. 1
Earlier bacterial classification is activated, use 5ml aseptic water washing inclined-plane again, form bacteria suspension, bacteria suspension inserts the 500ml triangular flask, this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours, and culture condition is 37 ℃, 180-200rpm detects cell concentration, reaches 10 in concentration
8The order of magnitude carries out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 10 in concentration
8The order of magnitude carries out next step;
Bacterium liquid in the 2000ml triangular flask is inserted 1m
3In the fermentor tank, the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m
3/ h cultivated 8 hours; Detect cell concentration, reach 10 in concentration
8The order of magnitude carries out next step;
With 1m
3Bacterium liquid in the fermentor tank inserts 5m
3Fermentor tank, access amount are 500 liters, and this fermentor tank contains 3m
3New 4 substratum, fermentation condition is: temperature 36-38 ℃, tank pressure 0.5kg/cm, air flow 120m
3/ h cultivated 15 hours.Detect cell concentration, reach 10 in concentration
9The order of magnitude stops fermentation.Mix with the high-quality peat composed of rotten mosses powder (commercially available) that carries out the dry sterilization postcooling in 1: 4 ratio then, carry out granulation again, oven drying at low temperature becomes the microbial strains agent.
Embodiment 3: the microbial strains agent that utilizes the traditional method preparation is not being carried out the detection of bacterium number under stock's the condition for a long time
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock's two weeks), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new 1 substratum (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We are not carrying out the detection of bacterium number under stock's the condition for a long time to the microbial strains agent that utilizes the traditional method preparation, and detected result is 6.6*10
8Individual/gram, (national standard is 2.0*10 for 3.3 times of national standard
8Individual/gram).Solved inoculum agent microbe number problem not up to standard.
Embodiment 4: the microbial strains agent that utilizes method preparation provided by the invention is not being carried out the detection of bacterium number under stock's the condition for a long time
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock's two weeks), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new 1 substratum (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We are not carrying out the detection of bacterium number under stock's the condition for a long time to the microbial strains agent that utilizes method preparation provided by the invention, and detected result is 8*10
8Individual/gram, be 4 times of national standard.Solved inoculum agent microbe number problem not up to standard.
Embodiment 5: the bacterium number is carried out in the microbial strains agent that utilizes the traditional method preparation detect under long-time stock's condition
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock 2 years), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new 1 substratum (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We carry out the detection of bacterium number to the microbial strains agent that utilizes the traditional method preparation under long-time stock's (2 years) condition, detected result is 6*10
8Individual/gram, be 3 times of national standard.Solved inoculum agent microbe number problem and the too short problem of preservation term not up to standard.
Embodiment 6: the bacterium number is carried out in the microbial strains agent that utilizes method preparation provided by the invention detect under long-time stock's condition
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock 2 years), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new 1 substratum (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We carry out the detection of bacterium number to the microbial strains agent that utilizes method preparation provided by the invention under long-time stock's (2 years) condition, detected result is 7.2*10
8Individual/gram, be 3.6 times of national standard.Solved inoculum agent microbe number problem and the too short problem of preservation term not up to standard.
Embodiment 7: the microbial strains agent of lengthy warehousing does not mix the effect that use the back with fertilizer
Chicken manure, ground corn core, cinder are mixed with 1: 2: 3 weight percent, adjusting water content is 30%, piled up 7-10 days under in temperature, the microbial strains agent of stock's two weeks is mixed with 1: 9 weight percent with it, make microbial fertilizer greater than 30 ℃ condition.
The microbial fertilizer of making is carried out on Different Crop and the fertile and of equal value conventional fertile sub-district fertilizer efficiency experiment relatively of matrix, 0.16 mu of each sub-district area, 3 repetitions are established in experiment.Execute 25 kilograms of microbial fertilizers for every mu during experiment; Matrix fertilizer (mix with 1: 2: 3 weight percent via chicken manure, ground corn core, cinder, after adjusting water content and being 30%, pile up under greater than 30 ℃ condition in temperature and to make in 7-10 days) is executed 25 kilograms for every mu; Conventional fertile (phosphoric acid diamino: urea: Repone K is mixing in 3: 3: 2) of equal value executes 25 kilograms for every mu.All, once executed, do not appended as base manure.Blank does not apply fertilizer.
Experimental result following (data are the volume increase per-cent of each fertilizer with respect to blank)
| Romaine lettuce | Wheat | Corn | Paddy rice | Cotton | Cucumber | |
| Microbial fertilizer | 10 | 14 | 15 | 20 | 15 | 35 |
| Matrix fertilizer | 1 | 7 | 9 | 9 | 8 | 25 |
| Of equal value conventional fertile | 3 | 7 | 8 | 5 | 6 | 18 |
The result shows that this microbial strains agent can finely play a role, and has good effect of increasing production.
Embodiment 8: the microbial strains agent of lengthy warehousing (2 years) mixes the effect that use the back with fertilizer
Chicken manure, ground corn core, cinder are mixed with 1: 2: 3 weight percent, adjusting water content is 30%, piled up 7-10 days under in temperature, the microbial strains agent in 2 years of stock is mixed with 1: 9 weight percent with it, make microbial fertilizer greater than 30 ℃ condition.
The microbial fertilizer of making is carried out on Different Crop and the fertile and of equal value conventional fertile sub-district fertilizer efficiency experiment relatively of matrix, 0.16 mu of each sub-district area, 3 repetitions are established in experiment.Execute 25 kilograms of microbial fertilizers for every mu during experiment; Matrix fertilizer (mix with 1: 2: 3 weight percent via chicken manure, ground corn core, cinder, after adjusting water content and being 30%, pile up under greater than 30 ℃ condition in temperature and to make in 7-10 days) is executed 25 kilograms for every mu; Conventional fertile (phosphoric acid diamino: urea: Repone K is mixing in 3: 3: 2) of equal value executes 25 kilograms for every mu.All, once executed, do not appended as base manure.Blank does not apply fertilizer.
Experimental result following (data are the volume increase per-cent of each fertilizer with respect to blank)
| Romaine lettuce | Wheat | Corn | Paddy rice | Cotton | Cucumber | |
| Microbial fertilizer | 7 | 12 | 13 | 14 | 10 | 30 |
| Matrix fertilizer | 1 | 7 | 9 | 9 | 8 | 25 |
| Of equal value conventional fertile | 3 | 7 | 8 | 5 | 6 | 18 |
The result shows that this microbial strains agent can be good at solving the quality guaranteed period problem, do not have marked difference by the fertilizer of its preparation with respect to the fertilizer of the microbial strains agent preparation of prolonged preservation not on result of use, has solved the present invention and has wanted the problem that solves.
Claims (11)
1, a kind of potassium bacterium is characterized in that its preserving number at China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC 1131.
2, a kind of potassium bacterial micro-organism inoculum agent is characterized in that this inoculum agent contains the described potassium bacterium of claim 1.
3, the described potassium bacterial micro-organism of claim 2 inoculum agent is characterized in that it is fermented through known microbial fermentation processes by the described potassium bacterium of claim 1 and further be processed into.
4, the described potassium bacterial micro-organism of claim 2 inoculum agent is characterized in that it is fermented through following steps by the described potassium bacterium of claim 1 and further be processed into:
1) preserve the inclined-plane certainly and get kind after, line inserts new 1 substratum, 30-38 ℃ of incubator leaves standstill under the dark condition to be cultivated 36 hours, and the composition of this new 1 substratum is that sucrose 5g/L, sal epsom 0.5g/L, lime carbonate 0.1g/L, sodium-chlor 1.0g/L, iron trichloride 0.005g/L, Sodium phosphate dibasic 2g/L, agar 1.5%-2.0%, pH transfer to 6.5-7.8;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of this new 1 liquid nutrient medium is that sucrose 5g/L, sal epsom 0.5g/L, lime carbonate 0.1g/L, sodium-chlor 1.0g/L, iron trichloride 0.005g/L, Sodium phosphate dibasic 2g/, pH transfer to 6.5-7.8;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of these new 2 substratum is that starch 10g/L, sucrose 10g/L, sal epsom 1g/L, sulfate of ammoniac 2g/L, Yeast diffusion juice 0.2g/L, lime carbonate 3g/L, sodium-chlor 1g/L, potassium primary phosphate 1g/L, pH transfer to 6.5-7.8;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of these new 3 substratum is that starch 10g/L, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.2g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1.5g/L, lime carbonate 1g/L, pH transfer to 6.5-7.8;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, the composition of these new 4 substratum is that starch 7g/L, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.1g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1g/L, lime carbonate 1g/L, pH transfer to 6.5-7.8.
5, a kind of microbial fertilizer is characterized in that this fertilizer contains the described potassium bacterium of claim 1.
6, the described microbial fertilizer of claim 5, it is characterized in that it be by claim 2 or 3 or 4 described potassium bacterial micro-organism inoculum agents through further processing, with known chemical fertilizer and or organic carrier mix.
7, a kind of method of producing the microbial strains agent is characterized in that having used the described potassium bacterium of claim 1 to ferment.
8, the described method of claim 7 is characterized in that the fermentation of the described potassium bacterium of claim 1 is carried out with known fermentation process.
9, the described method of claim 7 is characterized in that the fermentation of the described potassium bacterium of claim 1 is carried out by the following method:
1) preserve the inclined-plane certainly and get kind after, line inserts new 1 substratum, 30-38 ℃ of incubator leaves standstill under the dark condition to be cultivated 36 hours, and the composition of this new 1 substratum is that sucrose 5g/L, sal epsom 0.5g/L, lime carbonate 0.1g/L, sodium-chlor 1.0g/L, iron trichloride 0.005g/L, Sodium phosphate dibasic 2g/L, agar 1.5%-2.0%, pH transfer to 6.5-7.8;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of this new 1 liquid nutrient medium is that sucrose 5g/L, sal epsom 0.5g/L, lime carbonate 0.1g/L, sodium-chlor 1.0g/L, iron trichloride 0.005g/L, Sodium phosphate dibasic 2g/, pH transfer to 6.5-7.8;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of these new 2 substratum is that starch 10g/L, sucrose 10g/L, sal epsom 1g/L, sulfate of ammoniac 2g/L, Yeast diffusion juice 0.2g/L, lime carbonate 3g/L, sodium-chlor 1g/L, potassium primary phosphate 1g/L, pH transfer to 6.5-7.8;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of these new 3 substratum is that starch 10g/L, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.2g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1.5g/L, lime carbonate 1g/L, pH transfer to 6.5-7.8;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, the composition of these new 4 substratum is that starch 7g/L, sucrose 1g/L, Semen Maydis powder 1g/L, sal epsom 0.5g/L, Yeast diffusion juice 0.1g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 1g/L, lime carbonate 1g/L, pH transfer to 6.5-7.8.
10, a kind of method of producing microbial fertilizer is characterized in that having used the described potassium bacterium of claim 1.
11, the described method of claim 10, it is characterized in that it be by claim 2 or 3 or 4 described potassium bacterial micro-organism inoculum agents through further processing, with known chemical fertilizer and or organic carrier mix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410088855 CN1257267C (en) | 2004-11-08 | 2004-11-08 | Potassium bacteria and process for producing microorganism manure strain agent using said bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410088855 CN1257267C (en) | 2004-11-08 | 2004-11-08 | Potassium bacteria and process for producing microorganism manure strain agent using said bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1624111A CN1624111A (en) | 2005-06-08 |
| CN1257267C true CN1257267C (en) | 2006-05-24 |
Family
ID=34766115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410088855 Expired - Fee Related CN1257267C (en) | 2004-11-08 | 2004-11-08 | Potassium bacteria and process for producing microorganism manure strain agent using said bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1257267C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102424626A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof |
| CN102399710A (en) * | 2011-09-05 | 2012-04-04 | 麻林涛 | Brevibacillus laterosporus, microorganism strain agent and microorganism fertilizer prepared by using brevibacillus laterosporus as well as preparation methods thereof |
| CN102432362A (en) * | 2011-09-05 | 2012-05-02 | 麻林涛 | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof |
| CN105439724A (en) * | 2015-12-19 | 2016-03-30 | 佛山市艳晖生物科技有限公司 | Bacillus mucilaginosus bacterial fertilizer for farm onsite fermentation and applications thereof |
| CN107129939A (en) * | 2016-02-29 | 2017-09-05 | 株式会社可伶格林 | Produce the microorganism of chitosan catabolic enzyme and utilize its plant culture composition and cultural method |
-
2004
- 2004-11-08 CN CN 200410088855 patent/CN1257267C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1624111A (en) | 2005-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109022327B (en) | Preparation method of microbial mixed inoculant and application of microbial mixed inoculant in high-temperature composting | |
| CN1781881A (en) | Method for producing useful organic substance using industrial and agricultural organic wastes | |
| CN111073839B (en) | Siam bacillus, microbial inoculum and application thereof | |
| CN111363684B (en) | Composite microbial inoculum for efficiently degrading wood fibers and application thereof in composting | |
| CN111334452B (en) | Compound bacterium agent for degrading livestock and poultry manure and application thereof | |
| CN102703363A (en) | Bacillus methylotrophicus UTM401 and applications thereof | |
| CN101066897A (en) | K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith | |
| CN105543149B (en) | One plant of new bacillus megaterium and its application | |
| CN111533586B (en) | Chicken manure bio-organic fertilizer and preparation method thereof | |
| CN102424626A (en) | Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof | |
| CN114561327B (en) | Cellulose degradation composite microbial inoculant, and preparation method and application thereof | |
| CN109402014B (en) | Bacillus for producing cellulase and application thereof | |
| CN1257266C (en) | Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria | |
| CN102432362A (en) | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof | |
| CN1775035A (en) | Antago nistic fungus capable of preventing and treating continuous crops blight and its microbial organic fertilizer | |
| CN111154661B (en) | Complex microbial inoculant and application thereof | |
| CN1257267C (en) | Potassium bacteria and process for producing microorganism manure strain agent using said bacteria | |
| CN109517755B (en) | Acid-resistant bacillus licheniformis and application thereof in composting | |
| CN102399711B (en) | Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof | |
| CN116694526A (en) | Composite microbial agent for promoting rooting and preparation method thereof | |
| CN105219676A (en) | A kind of cultural method of bacillus laterosporus | |
| CN114045232B (en) | Organic material rapid fermentation synergistic microbial agent and application thereof | |
| CN105254424A (en) | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof | |
| CN113481111B (en) | Efficient biological straw fermentation inoculant and preparation method thereof | |
| CN105272406A (en) | Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060524 Termination date: 20211108 |