CN1257266C - Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria - Google Patents
Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria Download PDFInfo
- Publication number
- CN1257266C CN1257266C CN 200410088854 CN200410088854A CN1257266C CN 1257266 C CN1257266 C CN 1257266C CN 200410088854 CN200410088854 CN 200410088854 CN 200410088854 A CN200410088854 A CN 200410088854A CN 1257266 C CN1257266 C CN 1257266C
- Authority
- CN
- China
- Prior art keywords
- phosphorus
- substratum
- bacteria
- new
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 77
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 62
- 239000011574 phosphorus Substances 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 26
- 244000005700 microbiome Species 0.000 title claims abstract description 15
- 241000894006 Bacteria Species 0.000 title claims description 74
- 210000003608 fece Anatomy 0.000 title abstract description 12
- 239000010871 livestock manure Substances 0.000 title abstract description 12
- 230000000813 microbial effect Effects 0.000 claims description 94
- 239000003337 fertilizer Substances 0.000 claims description 63
- 238000000855 fermentation Methods 0.000 claims description 22
- 230000004151 fermentation Effects 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 19
- 239000001888 Peptone Substances 0.000 claims description 17
- 108010080698 Peptones Proteins 0.000 claims description 17
- 235000019319 peptone Nutrition 0.000 claims description 17
- 239000000725 suspension Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 14
- 239000002054 inoculum Substances 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 11
- 229920002472 Starch Polymers 0.000 claims description 11
- 239000008107 starch Substances 0.000 claims description 11
- 235000019698 starch Nutrition 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 11
- 229910019142 PO4 Inorganic materials 0.000 claims description 9
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 229930006000 Sucrose Natural products 0.000 claims description 9
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 9
- 229940095054 ammoniac Drugs 0.000 claims description 9
- 238000009792 diffusion process Methods 0.000 claims description 9
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 9
- 239000010452 phosphate Substances 0.000 claims description 9
- 239000011591 potassium Substances 0.000 claims description 9
- 229910052700 potassium Inorganic materials 0.000 claims description 9
- 239000005720 sucrose Substances 0.000 claims description 9
- 244000068988 Glycine max Species 0.000 claims description 8
- 235000010469 Glycine max Nutrition 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 29
- 238000004519 manufacturing process Methods 0.000 abstract description 25
- 230000000694 effects Effects 0.000 abstract description 11
- 238000011161 development Methods 0.000 abstract description 10
- 238000007689 inspection Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 12
- 239000000047 product Substances 0.000 description 11
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 8
- 241000726221 Gemma Species 0.000 description 8
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 238000002156 mixing Methods 0.000 description 7
- 230000014670 detection of bacterium Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000011159 matrix material Substances 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 230000007423 decrease Effects 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 241000287828 Gallus gallus Species 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 239000003818 cinder Substances 0.000 description 4
- 230000015271 coagulation Effects 0.000 description 4
- 238000005345 coagulation Methods 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 238000012262 fermentative production Methods 0.000 description 4
- 238000009413 insulation Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 241000195940 Bryophyta Species 0.000 description 3
- 238000005056 compaction Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 238000005469 granulation Methods 0.000 description 3
- 230000003179 granulation Effects 0.000 description 3
- 238000000643 oven drying Methods 0.000 description 3
- 239000003415 peat Substances 0.000 description 3
- 235000017060 Arachis glabrata Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 235000018262 Arachis monticola Nutrition 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 244000106835 Bindesalat Species 0.000 description 2
- 235000000318 Bindesalat Nutrition 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
- 244000302544 Luffa aegyptiaca Species 0.000 description 2
- 235000009814 Luffa aegyptiaca Nutrition 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000012204 lemonade/lime carbonate Nutrition 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- FAIAAWCVCHQXDN-UHFFFAOYSA-N phosphorus trichloride Chemical compound ClP(Cl)Cl FAIAAWCVCHQXDN-UHFFFAOYSA-N 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000009329 organic farming Methods 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The technical scheme provided by the present invention belongs to a microorganism manure field in an organism manure field. During recent years, the sample qualified rate of microorganism manure is lower than 60% according to national united inspection. Product quality mainly has the problems that effective bacterial amount does not reach the standard, guarantee periods are too short, surviving capability is reduced and surviving time is shortened in a storing process after strain is prepared into microorganism strain agents; in the process of production and use, the problems are represented in that strain activity is reduced, production performance is weakened, propagation speed is slowed and stress resistance is poor. The strain problems always obsess the development of the microorganism manure. The new phosphorus strain provided by the present invention and the microorganism strain agents produced by the new technical scheme can better solve the problems that the strain amount does not reach the standard and the guarantee periods are not extended, and the present invention is very important and significant.
Description
Technical field
Technical solutions according to the invention belong to the agricultural fertilizer field, concretely, are the microbial fertilizer fields that belongs in the biological fertilizer field, according to International Patent Classification, belong to C05F 11/08 field.
Background technology
China has a large population, ploughs few, and along with successively decreasing year by year in the development arable land in population growth, industry and city.According to the statistics made by the departments concerned, China's population increases by 1,600 ten thousand every year on average, and 4,000,000 mu of the annual minimizings of ploughing.Nineteen fifty population 5.5 hundred million, plough 16.5 hundred million mu, 2.99 mu of per capita cultivated lands, the per capita cultivated land was 1.51 mu in 1980, the nineteen ninety per capita cultivated land drops to 1.1 mu, is significantly less than 4.03 mu of world per capita cultivated lands' level, also is lower than 2.1 mu of Asia per capita cultivated lands' level.For this reason, unique feasible way of China's increase agricultural output is to increase the yield per unit area.Could satisfy the demand of industrial development and population increase to agricultural-food, chemical fertilizer is also with regard to the natural widespread use that obtained.
The soil compaction that unreasonable for a long time use chemical fertilizer brings, soil fertility decline, ecological damage, environmental pollution and agricultural byproducts quality decline problem have been subjected to the great attention of national departments concerned and agricultural science and technology circle.Using in a large number of chemical fertilizer caused soil fertility decline, the disadvantage that soil compaction and agriculture production cost improve constantly.In recent decades, China makes great efforts to explore the rational application of fertilizer always, overcomes the drawback of chemical fertilizer and the effective way of raising chemical fertilizer utilization ratio.
Agricultural fertilizer comprises chemical fertilizer and bio-feritlizer, is the irreplaceable important production means of China's agriculture production.Wherein, bio-feritlizer is as the important branch of agro-biological engineering subject and the important component part of soil fertilizer science, develops rapidly all having obtained aspect fundamental research and the applied research in nearly ten years.And the microbial fertilizer in the agricultural fertilizer (belonging to a kind of of bio-feritlizer) more and more is familiar with by people as a kind of important effect of agrotechnical measure in the development an agriculture featuring high yields, fine quality and high efficiency, this provides sizable development space for its exploitation, popularization, application, particularly the irreplaceability in development Organic farming, production A level, AA level green food has demonstrated fully its potential glamour.
China's microbial fertilizer development in recent years is very fast, has many enterprises to be engaged in production and operation, and kind constantly increases, and also has some external products to squeeze into the domestic market simultaneously.Owing to reasons such as bacterial classifications, the quality product of existing market is uneven, has occurred some problems in the production application.According to Ministry of Agriculture's random checking recent years, tracking selective examination, the situation of country's system inspection is seen, spot-check qualification rate less than 60%, and product quality problem mainly is that effective bacterium number is not up to standard, and the quality guaranteed period is too short.Living bacteria count is the important indicator of quality product in the microbial fertilizer, living bacteria count is not up to standard, quality product just can not get guaranteeing, what reflect is the effect instability, wherein reason mainly is the bacterial classification problem, bacterial classification is after being prepared as the microbial strains agent, and the survival ability in preserving process descends, and the survival time shortens.Show as bacterial activity and descend in the production use, production performance weakens, and reproduction speed is slack-off, and resistance is poor.Therefore, the bacterial classification problem perplexs the production and the development of China's microbial fertilizer always.Under these circumstances, bacterial classification provided by the invention and the microbial strains agent that utilizes this bacterial classification to produce can extraordinaryly solve the problem that the bacterium number is up to standard and the quality guaranteed period prolongs, and this makes the present invention seem very important and meaningful.
Summary of the invention
Microbial fertilizer is a kind of goods that cause obtaining the specific fertilizer effect with the effect of microbial life active, is a kind of of the fertilizer that uses in the agriculture production, and is different on main points and intension with traditional fertilizer and fertilizer.The microbial fertilizer production cost is low, and is easy to use, and effect is good, and is free from environmental pollution, not only volume increase after using, and can improve agricultural product quality and reduce the traditional fertilizer consumption.Microbial fertilizer is a fertilizer of producing green food, is the fertilizer of protection green environment, is rising fertilizer.This project is in new developmental stage in China at present.
The technical problem that solves
After soil compaction, soil fertility decline, ecological damage, environmental pollution and agricultural byproducts quality decline problem that national departments concerned is fully paid attention to using chemical fertilizer to bring, microbial fertilizer in the bio-feritlizer has obtained swift and violent development, but, owing to reasons such as bacterial classifications, the quality product of existing market is uneven, has occurred some problems in production application.According to Ministry of Agriculture's random checking recent years, tracking selective examination, the situation of country's system inspection is seen, spot-check qualification rate less than 60%, and product quality problem mainly is that effective bacterium number is not up to standard, and the quality guaranteed period is too short.Living bacteria count is the important indicator of quality product in the microbial fertilizer, living bacteria count is not up to standard, quality product just can not get guaranteeing, what reflect is that effect instability, quality guaranteed period are short, wherein reason mainly is the bacterial classification problem, bacterial classification is after being prepared as the microbial strains agent, and the survival ability in preserving process descends, and the survival time shortens.This problem is actually owing to the employed bacterial classification of these microbial strains agent or can not produces gemma, the ability that produces gemma is more weak, perhaps the gemma that produces is sprouted ability, can not finely spend stress conditions in the microbial strains agent production process and stock, preserving process, thereby showing as bacterial activity in the production use descends, production performance weakens, and reproduction speed is slack-off or the like.The bacterial classification problem perplexs the production and the development of China's microbial fertilizer always.Under these circumstances, novel bacterial provided by the invention and the microbial strains agent that utilizes new solution to produce can extraordinaryly solve the problem that the bacterium number is up to standard and the quality guaranteed period prolongs, and this makes the present invention seem very important and meaningful.
Technical scheme
At first, it should be explicitly made clear at this point that per-cent and the ratio mentioned among the present invention all are meant weight percent and part by weight.
The invention provides a kind of technical scheme that addresses the above problem, concrete, the invention provides a kind of microorganism (bacillus cereus, Bacillus cereus), concrete, bacterial classification be named as No. 1, phosphorus " new " (on April 15th, 2004 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, preserving number is CGMCC1132), this microorganism separates from high-yielding grain fields, and through domestication, proterties is stable for a long time.
The biological characteristics of this bacterial classification is that thalline is shaft-like, size 0.6-0.8*2-3 micron, Gram-positive, cellular plasm even dyeing, the flagellum adnation, give birth in the gemma or middle partially giving birth to, shape cylindricality or ellipse do not make volumination, bacterium colony is an oyster white on the beef peptone substratum, circle, edge irregular hair shape is outstanding, diameter 1-2 millimeter.Can utilize multiple nitrogenous source, carbon source, 37 ℃ of optimal pHs 7.1 of growth temperature 20-55 ℃ of optimum growth temp.Under bad condition, thalline is converted into gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature and drying to the poor environment strong stress resistance.
The applicant thinks, because bacterial classification is new, so the technical scheme that this bacterial classification uses traditional microbial strains agent and process for producing same to produce the microbial strains agent has novelty, (effectively the bacterium number is not up to standard if the microbial strains agent of producing can overcome above-mentioned shortcoming, quality guaranteed period is too short), have outstanding characteristics and obvious improvement, just had creativeness and practicality, had and be awarded Patent right primary condition.
Substratum that traditional microbial strains agent is used in producing and cultural method have detailed description in " phosphorus bacteria " volumes such as (, agriculture press, nineteen fifty-nine, Beijing) Chen Yanwei book.
Simultaneously, the applicant gropes the fermentation condition of No. 1, new phosphorus in the present invention, has drawn 1 and has been highly suitable for this culture of strains base and fermentation condition (this substratum and fermentation condition also are applicable to other part bacterial classifications).The applicant thinks, because bacterial classification is new, substratum provided by the invention and fermentation condition also are new, so the technical scheme that this bacterial classification uses new microbial fermentation condition provided by the invention to produce the microbial strains agent has novelty, if the microbial strains agent of producing can overcome above-mentioned shortcoming (effectively the bacterium number is not up to standard, and the quality guaranteed period is too short), have outstanding characteristics and obvious improvement, just had creativeness and practicality, had and be awarded Patent right primary condition.
This new substratum that culture scheme used has:
New No. 1 substratum of phosphorus (slant medium): peptone 5g/L, sodium-chlor 5g/L, extractum carnis 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8.
New No. 1 liquid nutrient medium of phosphorus: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8.
New No. 2 substratum of phosphorus: starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, extractum carnis 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8.
New No. 3 substratum of phosphorus: starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8.
New No. 4 substratum of phosphorus: starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8.
More than all substratum all before use through high pressure steam sterilization (121 ℃, 1.1kg/cm
3, 30 minutes), cooling.
The concrete mode of production of microbial strains agent:
One, culture presevation and activation culture
Bacterial classification is gone up preservation in streak culture mode at new No. 1 substratum of phosphorus (slant medium), and the short term storage condition is 4 ℃, activates per February 1 time; Long-term storage conditions is-20 ℃, activation in per 6 months 1 time.
Two, microorganism culturing
1, microorganism culturing (traditional microbial fermentation production method)
Substratum: starch 3%, soybean cake powder 1%, peptone 0.1%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, yeast powder 0.1%, lime carbonate 0.01%, PH7.2-7.4.
To insert behind the actication of culture in the above-mentioned substratum of 1000ml, triangular flask shakes bottle 160RPM 30-38 ℃ and cultivated 16 hours, be inoculated in the 200L seed fermentation jar by 1% inoculum size then and (still be substratum of the same race), ventilation, cultivated 16 hours for 30-38 ℃, receive (still being substratum of the same race) in the 2000L fermentor tank by 10% inoculum size again, cultivate for 30-38 ℃ and be warming up to 40-45 ℃ after 20 hours, treat to stop fermentation after 80% thalline produces gemma cultivation 10 hours.
2, microorganism culturing (method provided by the invention):
1) preserve the inclined-plane certainly and get kind after, line inserts slant medium (new No. 1 substratum of phosphorus), 30-38 ℃ of incubator left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in No. 1 liquid nutrient medium of new phosphorus of about 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in No. 2 substratum of new phosphorus of about 5-10 times volume, and it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in No. 3 substratum of new phosphorus of about 100-200 times volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in No. 4 substratum of new phosphorus of about 20-40 times volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude.
Three, the production of microbial strains agent
High-quality peat composed of rotten mosses powder (commercially available) is carried out the dry sterilization postcooling; join in the fermented liquid that fermentation ends (traditional microbial fermentation production method or production method provided by the invention) obtains according to the content of wanting to obtain microorganism in the microbial strains agent; fully stir; use the tablets press granulation; oven drying at low temperature; be packaged into Kucheng and be microbial strains agent product, mixing with common chemical fertilizer or organic carrier as required during use to become microbial fertilizer.
So, can utilize phosphorus bacteria provided by the invention, use known microbial fermentation processes to ferment and further be processed into the microbial strains agent.And can be with the phosphorus bacteria microbial strains agent of making through further processing, with known chemical fertilizer and or organic carrier mix microbial fertilizer.
Useful effect
Use the microbial strains agent of bacterial classification production provided by the present invention that higher effective bacterium number and long quality guaranteed period are arranged.
Use the traditional zymotic mode to carry out the inoculum agent of fermentative production at this bacterial classification, carry out effective bacterium number during warehouse-in and detect, its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effective bacterium number is 3.2 times of national standard.Solved strain number problem not up to standard.
The inoculum agent warehouse-in that uses fermentation mode provided by the invention to carry out fermentative production at this bacterial classification the time carries out effective bacterium number and detects, and its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effectively the bacterium number is 3.7 times of national standard.Also solved strain number problem not up to standard.
The inoculum agent that this bacterial classification uses the traditional zymotic mode to carry out fermentative production is preserved 2 years in the warehouse after, carry out effective bacterium number and detect, its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effective bacterium number is 2.95 times of national standard.Solved short problem of quality guaranteed period.
The inoculum agent that this bacterial classification uses fermentation mode provided by the invention to carry out fermentative production is preserved 2 years in the warehouse after, carry out effective bacterium number and detect, its result obviously is better than the effective bacterium number in commercially available other microbial strains agent, and effective bacterium number is 3.4 times of national standard.Solved short problem of quality guaranteed period.
Embodiment
Embodiment 1: utilize traditional method that new phosphorus is carried out the agent of fermentative preparation microbial strains for No. 1
Substratum: starch 3%, soybean cake powder 1%, peptone 0.1%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, yeast powder 0.1%, lime carbonate 0.01%, PH7.2-7.4.
To insert behind the actication of culture in the above-mentioned substratum of 1000ml, triangular flask shakes bottle 160RPM and cultivated 16 hours for 37 ℃, be inoculated in the 200L seed fermentation jar by 1% inoculum size then and (still be substratum of the same race), ventilation, cultivated 16 hours for 37 ℃, receive (still being substratum of the same race) in the 2000L fermentor tank by 10% inoculum size again, cultivate for 37 ℃ and be warming up to 42 ℃ after 20 hours, treat to stop fermentation after 80% thalline produces gemma cultivation 10 hours.Detect cell concentration, reach 10
9, mix with the high-quality peat composed of rotten mosses powder (commercially available) that carries out the dry sterilization postcooling by 1: 4 weight percent then, carry out granulation again, oven drying at low temperature becomes the microbial strains agent.
Embodiment 2: utilize method provided by the invention that new phosphorus is carried out the agent of fermentative preparation microbial strains for No. 1
Earlier bacterial classification is activated, use 5ml aseptic water washing inclined-plane again, form bacteria suspension, bacteria suspension inserts the 500ml triangular flask, this triangular flask contains No. 1 liquid nutrient medium of the new phosphorus of 100ml, uses shaking table to cultivate 18 hours, and culture condition is 37 ℃, 180-200rpm detects cell concentration, reaches 10 in concentration
8The order of magnitude carries out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains No. 2 substratum of the new phosphorus of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 10 in concentration
8The order of magnitude carries out next step;
Insert 1m with taking out bacterium liquid in the 2000ml triangular flask
3In the fermentor tank, the access amount is 3.5 liters, contains No. 3 substratum of the new phosphorus of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m
3/ h cultivated 8 hours; Detect cell concentration, reach 10 in concentration
8The order of magnitude carries out next step;
With 1m
3Bacterium liquid in the fermentor tank takes out and inserts 5m
3Fermentor tank, access amount are 130 liters, and this fermentor tank contains 3m
3New 4 substratum, fermentation condition is: temperature 36-38 ℃, tank pressure 0.5kg/cm, air flow 120m
3/ h cultivated 15 hours.Detect cell concentration, reach 10 in concentration
9The order of magnitude stops fermentation.Mix with the high-quality peat composed of rotten mosses powder (commercially available) that carries out the dry sterilization postcooling in 1: 4 ratio then, carry out granulation again, oven drying at low temperature becomes the microbial strains agent.
Embodiment 3: the microbial strains agent that utilizes the traditional method preparation is not being carried out the detection of bacterium number under stock's the condition for a long time
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock's two weeks), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new No. 1 substratum of phosphorus (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We are not carrying out the detection of bacterium number under stock's the condition for a long time to the microbial strains agent that utilizes the traditional method preparation, and detected result is 6.4*10
8Individual/gram, (national standard is 2.0*10 for 3.2 times of national standard
8Individual/gram).Solved inoculum agent microbe number problem not up to standard.
Embodiment 4: the microbial strains agent that utilizes method preparation provided by the invention is not being carried out the detection of bacterium number under stock's the condition for a long time
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock's two weeks), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new No. 1 substratum of phosphorus (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We are not carrying out the detection of bacterium number under stock's the condition for a long time to the microbial strains agent that utilizes method preparation provided by the invention, and detected result is 7.4*10
8Individual/gram/gram is 3.7 times of national standard.Solved inoculum agent microbe number problem not up to standard.
Embodiment 5: the bacterium number is carried out in the microbial strains agent that utilizes the traditional method preparation detect under long-time stock's condition
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock 2 years), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new No. 1 substratum of phosphorus (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We carry out the detection of bacterium number to the microbial strains agent that utilizes the traditional method preparation under long-time stock's (2 years) condition, detected result is 5.9*10
8Individual/gram, be 2.95 times of national standard.Solved inoculum agent microbe number problem and the too short problem of preservation term not up to standard.
Embodiment 6: the bacterium number is carried out in the microbial strains agent that utilizes method preparation provided by the invention detect under long-time stock's condition
According to the viable bacteria content in the dilution plate number scale detection microbial strains agent of routine.Take by weighing microbial strains agent 1 gram (stock 2 years), dissolve with sterilized water, being diluted to the thalline number with sterilized water is 20-100/ml, pipette the 1ml diluent then in sterile petri dish, add an amount of new No. 1 substratum of phosphorus (slant medium), the thorough mixing after coagulation, in 37 ℃ of insulation cans, be inverted and cultivated 48 hours, bacterium colony on the statistics flat board, more than the triplicate, multiply by this gram microbial strains dilution agent with mean value is the strain number that the milliliter number of cell concentration when being 20-100/ml is the agent of every gram microbial strains.We carry out the detection of bacterium number to the microbial strains agent that utilizes method preparation provided by the invention under long-time stock's (2 years) condition, detected result is 6.8*10
8Individual/gram, be 3.4 times of national standard.Solved inoculum agent microbe number problem and the too short problem of preservation term not up to standard.
Embodiment 7: the microbial strains agent of lengthy warehousing does not mix the effect that use the back with fertilizer
Chicken manure, ground corn core, cinder are mixed with 1: 2: 3 weight percent, adjusting water content is 30%, piled up 7-10 days under in temperature, the microbial strains agent of stock's two weeks is mixed with 1: 9 weight percent with it, make microbial fertilizer greater than 30 ℃ condition.
The microbial fertilizer of making is carried out on Different Crop and the fertile and of equal value conventional fertile sub-district fertilizer efficiency experiment relatively of matrix, 0.16 mu of sub-district area, 3 repetitions are established in experiment.Execute 25 kilograms of microbial fertilizers for every mu during experiment; Matrix fertilizer (mix with 1: 2: 3 weight percent via chicken manure, ground corn core, cinder, after adjusting water content and being 30%, pile up under greater than 30 ℃ condition in temperature and to make in 7-10 days) is executed 25 kilograms for every mu; Conventional fertile (phosphoric acid diamino: urea: phosphorus chloride is mixing in 3: 3: 2) of equal value executes 25 kilograms for every mu.All, once executed, do not appended as base manure.Blank does not apply fertilizer.
Experimental result following (data are the volume increase per-cent of each fertilizer with respect to blank)
| Romaine lettuce | Sponge gourd | Corn | Paddy rice | Peanut | Cucumber | |
| Microbial fertilizer | 14 | 25 | 15 | 14 | 17 | 30 |
| Matrix fertilizer | 1 | 19 | 10 | 9 | 10 | 25 |
| Of equal value conventional fertile | 3 | 18 | 8 | 5 | 11 | 17 |
The result shows that this microbial strains agent can finely play a role, and has good effect of increasing production.
Embodiment 8: the microbial strains agent of lengthy warehousing (2 years) mixes the effect that use the back with fertilizer
Chicken manure, ground corn core, cinder are mixed with 1: 2: 3 weight percent, adjusting water content is 30%, piled up 7-10 days under in temperature, the microbial strains agent in 2 years of stock is mixed with 1: 9 weight percent with it, make microbial fertilizer greater than 30 ℃ condition.
The microbial fertilizer of making is carried out on Different Crop and the fertile and of equal value conventional fertile sub-district fertilizer efficiency experiment relatively of matrix, 0.16 mu of sub-district area, 3 repetitions are established in experiment.Execute 25 kilograms of microbial fertilizers for every mu during experiment; Matrix fertilizer (mix with 1: 2: 3 weight percent via chicken manure, ground corn core, cinder, after adjusting water content and being 30%, pile up under greater than 30 ℃ condition in temperature and to make in 7-10 days) is executed 25 kilograms for every mu; Conventional fertile (phosphoric acid diamino: urea: phosphorus chloride is mixing in 3: 3: 2) of equal value executes 25 kilograms for every mu.All, once executed, do not appended as base manure.Blank does not apply fertilizer.
Experimental result following (data are the volume increase per-cent of each fertilizer with respect to blank)
| Romaine lettuce | Sponge gourd | Corn | Paddy rice | Peanut | Cucumber | |
| Microbial fertilizer | 13 | 24 | 13 | 13 | 16 | 30 |
| Matrix fertilizer | 1 | 19 | 10 | 9 | 10 | 25 |
| Of equal value conventional fertile | 3 | 18 | 8 | 5 | 11 | 17 |
The result shows that this microbial strains agent can be good at solving the quality guaranteed period problem, do not have marked difference by the fertilizer of its preparation with respect to the fertilizer of the microbial strains agent preparation of prolonged preservation not on result of use, has solved the present invention and has wanted the problem that solves.
Claims (11)
1, a kind of phosphorus bacteria is characterized in that its preserving number at China Committee for Culture Collection of Microorganisms common micro-organisms center is CGMCC1132.
2, a kind of phosphorus bacteria microbial strains agent is characterized in that this inoculum agent contains the described phosphorus bacteria of claim 1.
3, the described phosphorus bacteria microbial strains of claim 2 agent is characterized in that it is fermented through known microbial fermentation processes by the described phosphorus bacteria of claim 1 and further be processed into.
4, the described phosphorus bacteria microbial strains of claim 2 agent is characterized in that it is fermented through following steps by the described phosphorus bacteria of claim 1 and further be processed into:
1) preserve the inclined-plane certainly and get kind after, line inserts No. 1 substratum of new phosphorus, 30-38 ℃ of incubator leaves standstill under the dark condition to be cultivated 36 hours, and the composition of No. 1 substratum of this new phosphorus is that peptone 5g/L, sodium-chlor 5g/L, extractum carnis 5g/L, agar 1.5%-2.0%, pH transfer to 6.5-7.8;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in No. 1 liquid nutrient medium of new phosphorus of 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of No. 1 substratum of this new phosphorus is that peptone 5g/L, sodium-chlor 5g/L, extractum carnis 5g/L, pH transfer to 6.5-7.8;
4) bacteria suspension that step 3) is obtained joins in No. 2 substratum of new phosphorus of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of No. 2 substratum of this new phosphorus is that starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, extractum carnis 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfer to 6.5-7.8;
5) bacteria suspension that step 4) is obtained joins in No. 3 substratum of new phosphorus of 100-200 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of No. 3 substratum of this new phosphorus is that starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, pH transfer to 6.5-7.8;
6) bacteria suspension that step 5) is obtained joins in No. 4 substratum of new phosphorus of 20-40 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, the composition of No. 4 substratum of this new phosphorus is that starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfer to 6.5-7.8.
5, a kind of microbial fertilizer is characterized in that this fertilizer contains the described phosphorus bacteria of claim 1.
6, the described microbial fertilizer of claim 5, it is characterized in that it be by claim 2 or 3 or 4 described phosphorus bacteria microbial strains agent through further processing, with known chemical fertilizer and or organic carrier mix.
7, a kind of method of producing the microbial strains agent is characterized in that having used the described phosphorus bacteria of claim 1 to ferment.
8, the described method of claim 7 is characterized in that the fermentation of the described phosphorus bacteria of claim 1 is carried out with known fermentation process.
9, the described method of claim 7 is characterized in that the fermentation of the described phosphorus bacteria of claim 1 is carried out by the following method:
1) preserve the inclined-plane certainly and get kind after, line inserts No. 1 substratum of new phosphorus, 30-38 ℃ of incubator leaves standstill under the dark condition to be cultivated 36 hours, and the composition of No. 1 substratum of this new phosphorus is that peptone 5g/L, sodium-chlor 5g/L, extractum carnis 5g/L, agar 1.5%-2.0%, pH transfer to 6.5-7.8;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in No. 1 liquid nutrient medium of new phosphorus of 20 volumes, it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of No. 1 substratum of this new phosphorus is that peptone 5g/L, sodium-chlor 5g/L, extractum carnis 5g/L, pH transfer to 6.5-7.8;
4) bacteria suspension that step 3) is obtained joins in No. 2 substratum of new phosphorus of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of No. 2 substratum of this new phosphorus is that starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, extractum carnis 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfer to 6.5-7.8;
5) bacteria suspension that step 4) is obtained joins in No. 3 substratum of new phosphorus of 100-200 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude, the composition of No. 3 substratum of this new phosphorus is that starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, pH transfer to 6.5-7.8;
6) bacteria suspension that step 5) is obtained joins in No. 4 substratum of new phosphorus of 20-40 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, the composition of No. 4 substratum of this new phosphorus is that starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, sulfate of ammoniac 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfer to 6.5-7.8.
10, a kind of method of producing microbial fertilizer is characterized in that having used the described phosphorus bacteria of claim 1.
11, the described method of claim 10, it is characterized in that it be by claim 2 or 3 or 4 described phosphorus bacteria microbial strains agent through further processing, with known chemical fertilizer and or organic carrier mix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410088854 CN1257266C (en) | 2004-11-08 | 2004-11-08 | Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410088854 CN1257266C (en) | 2004-11-08 | 2004-11-08 | Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1624110A CN1624110A (en) | 2005-06-08 |
| CN1257266C true CN1257266C (en) | 2006-05-24 |
Family
ID=34766114
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410088854 Expired - Fee Related CN1257266C (en) | 2004-11-08 | 2004-11-08 | Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1257266C (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102838398A (en) * | 2011-09-05 | 2012-12-26 | 麻林涛 | Special compound microbial fertilizer for peanuts and method for preparing special compound microbial fertilizer |
| CN102399710A (en) * | 2011-09-05 | 2012-04-04 | 麻林涛 | Brevibacillus laterosporus, microorganism strain agent and microorganism fertilizer prepared by using brevibacillus laterosporus as well as preparation methods thereof |
| CN102417418A (en) * | 2011-09-05 | 2012-04-18 | 麻林涛 | Compound microbial fertilizer special for wheat and a preparation method thereof |
| CN102424626A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof |
| CN102976840A (en) * | 2011-09-05 | 2013-03-20 | 麻林涛 | Specialized composite microbial fertilizer for corn, and preparation method thereof |
| CN102432362A (en) * | 2011-09-05 | 2012-05-02 | 麻林涛 | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof |
| CN102424627A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Special compound microbial fertilizer for vegetables and preparation method thereof |
-
2004
- 2004-11-08 CN CN 200410088854 patent/CN1257266C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1624110A (en) | 2005-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109022327B (en) | Preparation method of microbial mixed inoculant and application of microbial mixed inoculant in high-temperature composting | |
| CN111073839B (en) | Siam bacillus, microbial inoculum and application thereof | |
| CN111363684B (en) | Composite microbial inoculum for efficiently degrading wood fibers and application thereof in composting | |
| CN102199050A (en) | Composite microbial fertilizer and preparation method thereof | |
| CN111334452B (en) | Compound bacterium agent for degrading livestock and poultry manure and application thereof | |
| CN101066897A (en) | K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith | |
| CN111533586B (en) | Chicken manure bio-organic fertilizer and preparation method thereof | |
| CN102424626A (en) | Microbial fertilizer prepared by bacillus laterosporus and preparation method thereof | |
| CN114561327B (en) | Cellulose degradation composite microbial inoculant, and preparation method and application thereof | |
| CN109402014B (en) | Bacillus for producing cellulase and application thereof | |
| CN109517755B (en) | Acid-resistant bacillus licheniformis and application thereof in composting | |
| CN111187739A (en) | Compound microbial preparation and culture method and application thereof | |
| CN1257266C (en) | Phosphorus bacteria and process for producing microorganism manure strain agent using said bacteria | |
| CN1775035A (en) | Antago nistic fungus capable of preventing and treating continuous crops blight and its microbial organic fertilizer | |
| CN102432362A (en) | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof | |
| CN111154661B (en) | Complex microbial inoculant and application thereof | |
| CN1257267C (en) | Potassium bacteria and process for producing microorganism manure strain agent using said bacteria | |
| CN105219676A (en) | A kind of cultural method of bacillus laterosporus | |
| CN102399711B (en) | Bacillus subtilis, microorganism strain agent and microorganism fertilizer prepared by using bacillus subtilis as well as preparation methods thereof | |
| CN105254424A (en) | Microbial fertilizer prepared from bacillus subtilis and preparation method thereof | |
| CN114045232B (en) | Organic material rapid fermentation synergistic microbial agent and application thereof | |
| CN113481111B (en) | Efficient biological straw fermentation inoculant and preparation method thereof | |
| CN105272406A (en) | Microbial fertilizer prepared by bacillus laterosporus and preparation method of microbial fertilizer | |
| CN102399710A (en) | Brevibacillus laterosporus, microorganism strain agent and microorganism fertilizer prepared by using brevibacillus laterosporus as well as preparation methods thereof | |
| CN109504623B (en) | Bacillus brevis thermophilus for producing amylase and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: WANG LI Owner name: BEIJING XINXIN SHENGDA BIOENGINEERING TECHNOLOGY C Free format text: FORMER OWNER: MA LINTAO Effective date: 20100928 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20100928 Address after: 100071 Beijing City, Fengtai District Science City 100 Road No. 6 hospital Baolong apartment No. A-2509 Patentee after: BEIJING XINXINSHENGDA BIOENGINEERING TECHNOLOGY Co.,Ltd. Address before: 100071, room 1, building 6, No. 2509, No. 100 Avenue, Fengtai District Science City, Beijing Co-patentee before: Wang Li Patentee before: Ma Lintao |
|
| PP01 | Preservation of patent right |
Effective date of registration: 20170125 Granted publication date: 20060524 |
|
| RINS | Preservation of patent right or utility model and its discharge | ||
| PD01 | Discharge of preservation of patent | ||
| PD01 | Discharge of preservation of patent |
Date of cancellation: 20170725 Granted publication date: 20060524 |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: BEIJING XINXINSHENGDA BIOENGINEERING TECHNOLOGY Co.,Ltd. Document name: Notification of Termination of Procedure |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: BEIJING XINXINSHENGDA BIOENGINEERING TECHNOLOGY Co.,Ltd. Document name: Notification to Pay the Fees |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200915 Address after: 450008 Floor 1603, No. 17, East Weisi Road, Jinshui District, Zhengzhou City, Henan Province Patentee after: Zhengzhou Ma doctor xiwanjia Biotechnology Co.,Ltd. Address before: 100071 Beijing City, Fengtai District Science City 100 Road No. 6 hospital Baolong apartment No. A-2509 Patentee before: BEIJING XINXINSHENGDA BIOENGINEERING TECHNOLOGY Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060524 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |