CN1605572A - Microbiological ammonia nitrogen aligning agent and method for making the same - Google Patents
Microbiological ammonia nitrogen aligning agent and method for making the same Download PDFInfo
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- CN1605572A CN1605572A CN 200310107801 CN200310107801A CN1605572A CN 1605572 A CN1605572 A CN 1605572A CN 200310107801 CN200310107801 CN 200310107801 CN 200310107801 A CN200310107801 A CN 200310107801A CN 1605572 A CN1605572 A CN 1605572A
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- ammonia nitrogen
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- Biological Treatment Of Waste Water (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
The present invention relates to one kind of microbial ammonia nitrogen regulator, and features that the microbial ammonia nitrogen regulator is prepared with composite bacteria preparation, culture medium, inorganic salt, organic carrier, water and other material and through composite fermentation, adding chemical matter and curing. The preparation process of the microbial ammonia nitrogen regulator features all the bacteria strains obtained with sewage or sludge and through induction mutation and culture, the simultaneous culture of all the bacteria strains, coating the microbes with natural polymer, composite fermentation process to obtain great amount of active metabolic matter, and composite fixing to fix several components on special carrier. The present invention is used in producing water of different quality.
Description
Technical field
The present invention relates to a kind of microorganism ammonia nitrogen conditioning agent and making method,, belong to the technology of waste water control field applicable to utilizing microorganism that sewage is handled.
Background technology
At present, disclosed patent, publication number is CN1348987A, the preparation method who is entitled as a kind of microorganism species of the mud of efficiently disposing of sewage, it is characterized in that: from sewage or mud, gather original strain, after separation and enrichment mixed culture, obtain engineering bacteria, add pending mud or sewage then and carry out strain domestication, cultivate the processing microbial inoculum that obtains enlarging after 10-20 days, every milliliter viable count is greater than 1,000,000,000.But this patent fails to illustrate the strain name and the kind of employing, and simultaneously the needed time in early stage is longer in actual use, and viable bacteria content is not high, because it be a liquid when handling mobile sewage, can be subjected to bigger influence.
Summary of the invention
The purpose of this invention is to provide a kind of probiotics count height, through composite curing and have the water conditioner-microorganism ammonia nitrogen conditioning agent of various active material.Be not only applicable to sewage disposal, the water that also can be used for aquaculture, aquarium or mobile water system is administered engineering.After the use, can decompose the various organic pollution materials in water body and the mud, purify water, reduce the ammonia nitrogen in the water body, the content formation of nitrite nitrogen, in water body, form benign microecological balance.
For realizing above purpose, technical scheme of the present invention provides a kind of microorganism ammonia nitrogen conditioning agent, it is characterized in that, separates from sewage work or Sewage outlet or organic sludge, utilizes the method strain screening of selection by mutation; Adopt compound culture technique, the many bacterial classifications that filter out are cultivated in the substratum of same special component; Utilize natural high molecular substance, wrap processed the bacterial classification in the composite fungus agent; By complex ferment technology, make and contain a large amount of living microorganisms in the product and by the physiological metabolism material of its generation; Utilize the composite curing technology at last, multiple composition is fixed on the special carrier, play consistently its effect.Raw material is made up of composite fungus agent, carbon source, nitrogenous source, inorganic salt, inductor, and it is prepared by following weight percentages:
Composite fungus agent 12-30%
Substratum 15-35%
Inorganic salt 0.02-1.8%
Organic carrier 30-40%
Water 20-30%
The optimum weight proportioning of each raw material of preparation of the present invention is:
Composite fungus agent 18%
Substratum 22%
Inorganic salt 0.06%
Organic carrier 35%
Water 24.94%
Composite bacteria of the present invention is bacillus (Bacillus sp.), Candida utilis (Candida utilis), Rhodopseudomonas palustris (Rhodopseudomonas palustris), reaches the mould genus of knurl spore (Sepedonium sp.), Geotrichum (Geotrichirm sp.), gives birth to one of a branch moving glue bacterium (Zoogloearamigera), denitrification thiobacillus (Thiobacillus denitrificans), nitrobacteria (Nitrobacter) or their mixture, and processed through bag; Described substratum is one of molasses, peptone, yeast extract paste, somatomedin solution or their mixture; Described inorganic salt are one of potassium primary phosphate, dipotassium hydrogen phosphate, magnesium chloride, ammonium sulfate, ammonium nitrate, sodium-chlor or their mixture; Described organic carrier is one of Semen Maydis powder, blue or green chaff, straw powder or their mixture.
Making method of the present invention is characterized in that, all bacterial classifications of employing all are to separate from sewage work or Sewage outlet or organic sludge, utilizes the method for selection by mutation to filter out; Adopted the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum; In composite fungus agent, add sodium alginate and zein, bacterial classification is wrapped processed; After fermenting, carry out composite curing and granulate with other batch mixes; It comprises the following steps:
(1) from sewage work or Sewage outlet or organic sludge, separates, utilize the method screening of selection by mutation;
(2) each bacterial classification after the purifying cultivation is cultivated in same substratum, substratum is the aqueous solution that contains peptone 0.2-0.8%, glucose 1.0-1.8%, sodium-chlor 0.01-0.07%, yeast extract paste 1.8-2.8%, sal epsom 0.01-0.06%, and the pH value is to 6.8-7.2; Culture temperature is that 20-32 ℃, time are 72-120 hour, obtains composite fungus agent;
(3) in composite fungus agent, add sodium alginate 2-5% and zein 4-7%, regulate pH value to 5.0, in closed tin, mix, rotating speed 65r/min, 35 ℃ of temperature promptly obtain after 10-15 hour through wrapping processed composite fungus agent;
(4) composite fungus agent added insert in the sealed fermenting jar after substratum, organic carrier, inorganic salt and water stir, 22-30 ℃ bottom fermentation 96-108 hour, obtain the work in-process of semi-fluid shape; And then the Na[AlSi about adding 16-25%
2O
2] H
2O, Ca (Al
2Si
3O
10H
2O, (CaNa
2) (Al
2Si
2O
8) H
2O carries out composite curing; After 20-30 hour, granulate and obtain microorganism ammonia nitrogen conditioning agent finished product.
This invention is suitable for all kinds of waste water, comprises deodorizing, purification and the harmless treatment of city domestic sewage, trade effluent, livestock-raising field waste water; The purification of water quality of city river, blowdown pipe network, man-made lake, swimming pool, boat paradise, aquafarm etc.; Purify the water pollution in rivers, lake.
The product of this patent invention belongs to biotechnological formulation, contain definite chemical composition, microorganism used therefor all belongs to the probiotics useful to the mankind, therefore, people, animal, plant are perfectly safe, without any side effects, antioxidant that contains on the contrary and several physiological active substances are to the animal in the processing environment, useful coordination and the promoter action of plant.
In sewage treatment process, according to the nutritional condition in the external world, carry out the adjusting of bacterium internal energy, when extraneous nutritive deficiency, the bacterium internal energy reduces, and surface charge reduces, and the bonding force between the bacterium forms the suede grain greater than repulsive force, forms zoogloea; When external nutrition is enriched (sewage mixes the initial stage with active sludge, and F/M is bigger), the suede grain of formation disperses again, and then decomposes the various organic substances in the sewage.The beta-hydroxy-butanoic acid of bacterium cylinder accumulation is discharged into liquid phase simultaneously, also can make aggegation mutually between bacterium, forms the suede grain, and the viscous substance that the microorganism process of ingesting discharges also can promote aggegation.
Microorganism absorption pollutent mainly is the ion exchange by cytolemma, one netted peptidoglycan structure is arranged on the gram-positive bacteria cell wall, and there are teichoic acid or teichuronic acid to connect, phosphatide on it or carboxyl make cell walls electronegative, to the stronger absorbing power of positively charged ion tool in the sewage, and with positively charged contaminant ion generation permutoid reaction, pollution substance can be entered in the cell.Some active groups on the cell walls also have stronger complexing action, with particularly some macromolecular material complexings of pollution substance, make pollutant sediment at cell surface, and then, process body secretion exoenzyme etc. makes pollutent take place to transform or degraded.
The physiologically active substances such as coenzyme that produce in the composite bacteria metabolic process and discharge pathogenic former in the water body, as the equal certain restraining effect of tool such as Aeromonas hydrophila, Edwardsiella, mould, also can with the actinomycetes symbiosis in the water, make in the waters profitable strain propagation, suppress other pernicious bacterias breedings.
The entire sewage treating processes is actually with coagulation-deamination-biochemical treatment process and finishes.After microorganism ammonia nitrogen conditioning agent is thrown in, under the aeration situation, form a large amount of zoogloeas, various organic suspension things in the active adsorption sewage, and simultaneously it is decomposed, by a series of effects such as oxidation, ammonification, nitrated, denitrification, phosphorus decomposing, sulfurations, various organism and harmful pollutants can be decomposed into carbonic acid gas, nitrate, vitriol etc., reduce organic content and ammonia-nitrogen content in the water body, other chemical ingredients of Jia Ruing also has deamination simultaneously, also can improve the water quality of water body when decomposing mud.
Beneficial microorganism in the microorganism ammonia nitrogen conditioning agent at first is a decomposer to the decomposition of fermenting of organic and other pollutents in the water body, makes it innoxious.Then, the synthesis type microorganism utilizes its degradation production again, as NH
3, H
2S, CH
4, CO
2Deng, carrying out organic synthesis, larger molecular organics matter that is synthesized and microorganism coprecipitation become mud in the bottom, reach solid and liquid separation state, thereby reach the purpose of water purification.
Using this technology, at first is to make that beneficial microorganism quantity heightens in the water body, forms good waters micro-ecological environment.Then be planktonic organism and zootic increasing such as red worm, nematode, finally reach healthy and have the purpose that the fine water of vitality forms.Present technique is purified waste water is to recover and fortified water inherent oneself detergent power at all, forms health and is rich in the good water area ecological environment of vitality.
Probiotics is in finishing the water purification process, and the physiologically active substance of generation and antioxidant etc. all have good effect to animal, plant.The safety and Health of purifying waste water that utilizes this technology to produce can be used as farming, woods, herds, secondary, fishing and other every profession and trade water, improves the regeneration rate of water.
Advantage of the present invention is:
1. at the organic pollution materials that causes the water pollution major cause, filter out the strong microorganism of multiple specificity, water body is carried out the comprehensive regulation;
2. developed the compound cultivation technology of bacterial classification, made microorganism growth and breeding under same culture condition of multiple different genera;
3. utilize the composite curing technology, multiple composition is fixed on the special carrier, and made microorganism and metabolite mass-energy thereof in a relatively long time, play consistently effect, can be applicable to the processing of mobile sewage;
4. when water body is purified, can also effectively decompose mud, eliminate the root that causes water pollution;
5. applied widely, be suitable for all kinds of waste water, comprise deodorizing, purification and the harmless treatment of city domestic sewage, trade effluent, livestock-raising field waste water; The purification of water quality of city river, blowdown pipe network, man-made lake, swimming pool, boat paradise, aquafarm etc.; Purify the water pollution in rivers, lake;
6. the product of this patent invention belongs to biotechnological formulation, contain definite chemical composition, microorganism used therefor all belongs to the probiotics useful to the mankind, therefore, people, animal, plant are perfectly safe, without any side effects, antioxidant that contains on the contrary and several physiological active substances are to the animal in the processing environment, useful coordination and the promoter action of plant.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail:
Bacterial classification assembly in the composite fungus agent:
From sewage work or Sewage outlet or organic sludge, separate, after utilizing the method separation screening of selection by mutation, take by weighing by following weight proportion:
Bacillus (Bacillus sp.) 24%, Candida utilis (Candida utilis) 18%, Rhodopseudomonas palustris (Rhodopseudomonas palustris) 20%, the mould genus of knurl spore (Sepedoniumsp.) 8%, Geotrichum (Geotrichirm sp.) 8%, the living moving glue bacterium of branch (Zoogloea ramigera) 10%, denitrification thiobacillus (Thiobacillus denitrificans) 7%, nitrobacteria (Nitrobacter) 5%
The making method of composite fungus agent:
Take by weighing substratum by following weight proportion: peptone 0.5%, glucose 1.5%, sodium-chlor 0.04%, yeast extract paste 2.4%, sal epsom 0.03%, the pH value is adjusted to 6.8
Each bacterial classification after the purifying cultivation is cultivated in above substratum; Culture temperature is that 28 ℃, time are 96 hours, obtains composite fungus agent; Add 3% sodium alginate and 5.5% zein, regulate pH value to 5.0, in closed tin, mix, rotating speed 65r/min, 35 ℃ of temperature promptly obtained through wrapping processed composite fungus agent after 12 hours.
Product processes takes by weighing composite fungus agent and raw material by following weight proportion:
Composite fungus agent 18%, substratum 22%, inorganic salt 0.06%, wherein ammonium sulfate 10%, dipotassium hydrogen phosphate 14%, potassium primary phosphate 10%, magnesium chloride 20%, ammonium nitrate 15%, sodium-chlor 31%; Organic carrier 35%, water 24.94%.
Above raw material stirring is inserted in the sealed fermenting jar after evenly, and 28 ℃ of bottom fermentations 100 hours obtain the work in-process of semi-fluid shape; And then add Na[AlSi about 20%
2O
2] H
2O, Ca (Al
2Si
3O
10H
2O, (CaNa
2) (Al
2Si
2O
8) H
2O carries out composite curing.After 24 hours, granulate and obtain microorganism ammonia nitrogen conditioning agent finished product.
In use, use, use 1 kilogram by per 20 square metres, the dark water surface of 1-1.5 rice generally speaking according to the water quality situation of sewage; When being applied to aquaculture or landscape water, use the 3-5 kilogram by 1 meter every mu of dark water surface.
Claims (7)
1. microorganism ammonia nitrogen conditioning agent is characterized in that it is prepared by following weight percentages:
Composite bacteria 12-30%
Substratum 15-35%
Inorganic salt 0.02-1.8%
Organic carrier 30-40%
Water 20-30%
2. a microorganism ammonia nitrogen conditioning agent is characterized in that, wherein the optimum weight proportioning of each raw material is:
Composite bacteria 18%
Substratum 22%
Inorganic salt 0.06%
Organic carrier 35%
Water 24.94%
3. microorganism ammonia nitrogen conditioning agent according to claim 1, it is characterized in that, described composite bacteria is bacillus (Bacillus sp.), Candida utilis (Candida utilis), Rhodopseudomonas palustris (Rhodopseudomonas palustris), and the mould genus of knurl spore (Sepedonium sp.), Geotrichum (Geotrichirm sp.), give birth to branch moving glue bacterium (Zoogloea ramigera), denitrification thiobacillus (Thiobacillus denitrificans), one of nitrobacteria (Nitrobacter) or their mixture, and processed through bag.
4. microorganism ammonia nitrogen conditioning agent according to claim 1 is characterized in that, described substratum is one of molasses, peptone, yeast extract paste, somatomedin solution or their mixture.
5. microorganism ammonia nitrogen conditioning agent according to claim 1 is characterized in that, described inorganic salt are one of potassium primary phosphate, dipotassium hydrogen phosphate, magnesium chloride, ammonium sulfate, ammonium nitrate, sodium-chlor or their mixture.
6. microorganism ammonia nitrogen conditioning agent according to claim 1 is characterized in that, described organic carrier is one of Semen Maydis powder, blue or green chaff, straw powder or their mixture.
7. the making method of microorganism ammonia nitrogen conditioning agent according to claim 1 is characterized in that, all bacterial classifications of employing all are to separate from sewage work or Sewage outlet or organic sludge, utilizes the method for selection by mutation to filter out; Adopted the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum; In composite fungus agent, add sodium alginate and zein, bacterial classification is wrapped processed; After fermenting, carry out composite curing and granulate with other batch mixes; It comprises the following steps:
(1) from sewage work or Sewage outlet or organic sludge, separates, utilize the method screening of selection by mutation;
(2) each bacterial classification after the purifying cultivation is cultivated in same substratum, substratum is the aqueous solution that contains peptone 0.2-0.8%, glucose 1.0-1.8%, sodium-chlor 0.01-0.07%, yeast extract paste 1.8-2.8%, sal epsom 0.01-0.06%, and the pH value is to 6.8-7.2; Culture temperature is that 20-32 ℃, time are 72-120 hour, obtains composite fungus agent;
(3) in composite fungus agent, add sodium alginate 2-5% and zein 4-7%, regulate pH value to 5.0, in closed tin, mix, rotating speed 65r/min, 35 ℃ of temperature promptly obtain after 10-15 hour through wrapping processed composite fungus agent;
(4) composite fungus agent added insert in the sealed fermenting jar after substratum, organic carrier, inorganic salt and water stir, 22-30 ℃ bottom fermentation 96-108 hour, obtain the work in-process of semi-fluid shape; And then the Na[AlSi of adding 16-25%
2O
2] H
2O, Ca (Al
2Si
3O
10H
2O, (CaNa
2) (Al
2Si
2O
8) H
2O carries out composite curing; After 20-30 hour, granulate and obtain microorganism ammonia nitrogen conditioning agent finished product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310107801 CN1257113C (en) | 2003-10-08 | 2003-10-08 | Microbiological ammonia nitrogen aligning agent and method for making the same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310107801 CN1257113C (en) | 2003-10-08 | 2003-10-08 | Microbiological ammonia nitrogen aligning agent and method for making the same |
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| CN1605572A true CN1605572A (en) | 2005-04-13 |
| CN1257113C CN1257113C (en) | 2006-05-24 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100343184C (en) * | 2005-12-09 | 2007-10-17 | 汪承保 | High-efficient fast ammonia nitragen rmoving agent |
| CN100400440C (en) * | 2006-01-24 | 2008-07-09 | 沈阳大学 | Efficient ecological sewage treating promoter and its prepn process |
| CN102531195A (en) * | 2011-05-31 | 2012-07-04 | 武汉华科特新技术有限公司 | High-efficiency autotrophic denitrification water quality regulation bacterium agent and application thereof |
| CN104276665A (en) * | 2013-11-16 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for snakehead culture water and preparation method thereof |
| CN104276664A (en) * | 2013-11-15 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for hairy crab culture water and preparation method thereof |
| CN105399269A (en) * | 2015-10-27 | 2016-03-16 | 桂林瑞丰环保微生物应用研究所 | Dual-aeration gained microbiological treatment method for industrial wastewater |
| CN105417837A (en) * | 2015-10-28 | 2016-03-23 | 桂林瑞丰环保微生物应用研究所 | Method for treating industrial wastewater by micro-biological degradation |
| CN108249588A (en) * | 2018-02-08 | 2018-07-06 | 上海山恒生态科技股份有限公司 | A kind of composite bacteria agent formula for reducing water body ammonia-nitrogen content |
| CN113943671A (en) * | 2021-10-24 | 2022-01-18 | 甘肃省科学院生物研究所 | Improved compound microbial agent and application thereof in yield increase of angelica sinensis |
| CN114230019A (en) * | 2021-10-25 | 2022-03-25 | 盐城工学院 | Application of probiotic activated sludge in sewage treatment |
| CN115159680A (en) * | 2020-05-21 | 2022-10-11 | 浙江海元环境科技有限公司 | Composite sludge strain for yellow rice wine wastewater treatment and wastewater treatment method |
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2003
- 2003-10-08 CN CN 200310107801 patent/CN1257113C/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100343184C (en) * | 2005-12-09 | 2007-10-17 | 汪承保 | High-efficient fast ammonia nitragen rmoving agent |
| CN100400440C (en) * | 2006-01-24 | 2008-07-09 | 沈阳大学 | Efficient ecological sewage treating promoter and its prepn process |
| CN102531195B (en) * | 2011-05-31 | 2016-04-27 | 武汉华科特新技术有限公司 | A kind of autotrophic denitrification water quality regulation bacterium agent and application thereof |
| CN102531195A (en) * | 2011-05-31 | 2012-07-04 | 武汉华科特新技术有限公司 | High-efficiency autotrophic denitrification water quality regulation bacterium agent and application thereof |
| CN104276664A (en) * | 2013-11-15 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for hairy crab culture water and preparation method thereof |
| CN104276665A (en) * | 2013-11-16 | 2015-01-14 | 上海葵亚环保科技有限公司 | Microbial water purifying agent for snakehead culture water and preparation method thereof |
| CN105399269A (en) * | 2015-10-27 | 2016-03-16 | 桂林瑞丰环保微生物应用研究所 | Dual-aeration gained microbiological treatment method for industrial wastewater |
| CN105417837A (en) * | 2015-10-28 | 2016-03-23 | 桂林瑞丰环保微生物应用研究所 | Method for treating industrial wastewater by micro-biological degradation |
| CN108249588A (en) * | 2018-02-08 | 2018-07-06 | 上海山恒生态科技股份有限公司 | A kind of composite bacteria agent formula for reducing water body ammonia-nitrogen content |
| CN115159680A (en) * | 2020-05-21 | 2022-10-11 | 浙江海元环境科技有限公司 | Composite sludge strain for yellow rice wine wastewater treatment and wastewater treatment method |
| CN115159680B (en) * | 2020-05-21 | 2024-02-13 | 浙江海元环境科技有限公司 | Composite sludge strain for yellow wine wastewater treatment and wastewater treatment method |
| CN113943671A (en) * | 2021-10-24 | 2022-01-18 | 甘肃省科学院生物研究所 | Improved compound microbial agent and application thereof in yield increase of angelica sinensis |
| CN113943671B (en) * | 2021-10-24 | 2022-07-19 | 甘肃省科学院生物研究所 | Improved compound microbial agent and application thereof in yield increase of angelica sinensis |
| CN114230019A (en) * | 2021-10-25 | 2022-03-25 | 盐城工学院 | Application of probiotic activated sludge in sewage treatment |
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|---|---|
| CN1257113C (en) | 2006-05-24 |
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