CN1602723A - Grouper bait and method for making same - Google Patents

Grouper bait and method for making same Download PDF

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Publication number
CN1602723A
CN1602723A CNA2004100520445A CN200410052044A CN1602723A CN 1602723 A CN1602723 A CN 1602723A CN A2004100520445 A CNA2004100520445 A CN A2004100520445A CN 200410052044 A CN200410052044 A CN 200410052044A CN 1602723 A CN1602723 A CN 1602723A
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animal protein
phagostimulant
enzymolysis
raw material
antioxidant
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CN1281149C (en
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刘永坚
田丽霞
王胜
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Sun Yat Sen University
National Sun Yat Sen University
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National Sun Yat Sen University
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Abstract

The invention pertains a kind of phagostimulant to rockfishes and other fishes, and its produce way. Materials by weight: animal protease 70-85%, nucleotide 2-10%, sulphocompound 0.4-3%, complex aminoacid 5-15%, betaine 2-20%, pigment 0.5-5%, oxidation preventive 0.1-0.5%. Production way: add animal protease after enzymolysis in oxidation preventive of 0.5-3% of fresh animal protease by weight; add by rate nucleotide, sulphocompound, complex aminoacid, betaine, pigment, oxidation preventive, mix and dry them, then the phagostimulant is made over. This phagostimulant can absorb sober of water animal effectually, promote growing, increase the ability of defeat disease, and increase the rate of living.

Description

A kind of grouper phagostimulant and preparation method thereof
Technical field
A kind of efficient phagostimulant that provides for aquiculture animals such as groupers and preparation method thereof is provided.
Technical background
Famous and precious economic fish grouper meat flavour deliciousness in the aquaculture, economic worth is very high, especially in Southeast Asia, Japan and Hongkong and Macro market, cost an arm and a leg, be a kind of marine product of top grade.Along with people grow with each passing day to the demand of fresh and alive marine product, more and more supply falls short of demand in market.Lot of domestic and international country all carries out propagating artificially of seawater economic animals such as grouper positive.The universal demand of seedling that makes of culturing is increasing, but the wild seedling limited amount, artificial breeding of seawater economic animals such as grouper and adult fish culture are the fairly obvious high-valued technical industries of economic benefit, have vast market prospect.
Phagostimulant belongs to a kind of non-nutritive additive, in aquatic feeds, add the palatability that can improve feed in right amount, improve the bait taste, can stimulate sense of smell, vision and the sense of taste of aquiculture animal, make they accumulate in bait around, thereby improve the appetite of aquatic livestock, keep high nutrition and take in, change the situation of ingesting under disease or the stressed condition, promote ingesting of aquatic livestock, strengthen the immunity of animal, alleviate some state of an illness, breeding fish for health provides safeguard; And can strengthen aquatic livestock to the digesting and assimilating and promote growth of artitificial food, improve feed efficiency, shorten the time of searching for food, reduce feed waste, keep the clean of water body environment, favourable greatly to the health of aquatic cultivated animals.In addition, the development and use of phagostimulant can improve the local flavor of fish meal-less feed, solve protein feeds problem in short supply, for the development and use of new feedstuff resource provide wide prospect.Phagostimulant should have good stable usually.Quality after storing, process and processing should be stablized, and simultaneously, bad variation should not take place between phagostimulant and other raw materials, additive.Ester class and aromatic series aldehydes have been subjected to very big restriction in use owing to very unstable to alkali.Phagostimulant itself is wanted uniformity, and favorable dispersibility, adsorptivity and absorption are normal, and safe, nontoxic.As the contaminant water (sawdust) not only of some composition in the bait additive of Chinese patent application number 93105426.5 and 95102782.4 documents record, and easily nourish bacterium (bright cow dung).Also some mainly is exactly to extract the composition with food calling effect with the method for chemistry from animal and plant.But many shortcomings are also arranged, and as the food ration of animal, trophic level and bait utilization are not high.Single L-amino acid or its mixture also once became the food calling additive of fishes and shrimps, but owing to its cost is low, commodity price is higher its use is restricted, so the phagostimulant that food calling is effective, cost is low become problem and the research and development focus that faces the needs solution.More than all problems all need further to research and develop.
Summary of the invention
The purpose of this invention is to provide a kind of efficient phagostimulant that food calling is effective, cost is low that is suitable for aquiculture animals such as grouper and preparation method thereof, to overcome the above-mentioned shortcoming of existing in prior technology, solve the ubiquitous problem of current feeding additive aquatic animal (phagostimulant).
Grouper phagostimulant of the present invention be a kind of serve as the absorption main body with animal protein enzymolysis thing dry powder, make even distribution and dry blended composition through stirring after adding materials such as nucleotides, sulfur-containing compound, compound amino acid, betaine and pigment.This phagostimulant is rich in protein, polypeptide, free amino acid etc., and have materials such as certain aliphatic acid, nucleotides and alkaloid, produce strong physical stimulation and chemical stimulation from aspects such as vision, sense of smell, the sense of taste to the marine cultured animal fry, fry is had strong bait food calling effect.Raw materials used economy is easy to get, and processing method meets sanitary standard, is a kind of green additive safely and efficiently.
Phagostimulant of the present invention, its one-tenth are grouped into and proportioning is by weight: animal protein enzymolysis thing dry powder 70-85%, nucleotides 2-10%, sulfur-containing compound 0.4-3%, compound amino acid 5-15%, betaine 2-20%, pigment 0.5-5% and antioxidant 0.1-0.5%.Described nucleotides can be to be selected from inosinicacid, adenosine diphosphate, the adenosine triphosphate one or more; Described sulfur-containing compound can be to be selected from dimethyl-β-DMPT (DMPT), methyl-sulfoxide (DM), the allicin one or more; Described compound amino acid can be to be selected from glycine (Gly), taurine (Tau), alanine (Phe), glutamic acid (Glu), methionine (Met), valine (Val), proline (Pro), arginine amino acid such as (Arg) and the glutathione (GSH) two or more; Described pigment can be that astaxanthin is or/and vitamin B2; Described antioxidant can be to be selected from vitamin E, vitamin C phosphoric ester, the ethoxyquinoline one or more.
Animal protein enzymolysis thing dry powder used in the phagostimulant of the present invention can add the antioxidant that accounts for fresh animal protein sources raw material weight 0.5-3%, spray-dried obtaining by fresh animal protein sources raw material behind protease hydrolyzed.
The phagostimulant of the invention described above can prepare by the following method, the steps include:
(1) with fresh animal protein source raw material, according to expecting water weight ratio=1: ratio (2-6) is mixed, and regulates pH8.5-9.5, and the alkali extraction is carried out in homogenate, crosses 60-100 mesh sieve removal partial impurities; Add protease, regulate 40-70 ℃ of temperature, and regulate suitable pH value according to the kind of used protease; 50-70 ℃ of constant temperature enzymolysis is 1-10 hour in Cooling or heating jar, heats to 80-95 ℃ then and keeps 10-15 minute so that enzyme deactivation is then lowered the temperature back 40-70 ℃, adds exopeptidase enzymolysis 1-10 hour again with the elimination bitter taste; Intact back adds the antioxidant that accounts for fresh animal protein sources raw material weight 0.5-3%, filter hydrolyzate, through concentrating, spray-drying gets required animal protein enzymolysis thing dry powder then.Shower nozzle rotating speed during spray-drying is generally 8000-22000r/min (being preferably 12000-20000r/min), and inlet temperature is 150-230 ℃, and outlet temperature is 90-110 ℃; Described protease is generally one or more in trypsase, pepsin, chymotrypsin, papain, bromelain, the ficin; Exopeptidase is generally Aminopeptidase P or/and carboxypeptidase; The protease consumption is generally every gram bright protein sources raw material 5000-30000IU or every milliliter of enzymolysis liquid 50-500IU; The exopeptidase consumption is generally every gram bright protein sources raw material 5000-30000IU or every milliliter of enzymolysis liquid 50-500IU;
(2) the animal protein enzymolysis thing dry powder with gained is absorption carrier, adds nucleotides, sulfur-containing compound, compound amino acid, betaine, pigment and antioxidant in proportion, through mixing, promptly obtaining required phagostimulant after the drying.
Animal protein source raw material described in the invention described above method can be selected from one or more in the cheap animal protein raw materials such as mussel meat, cuttlefish meat, red worm, seawater iced trash fish.Described antioxidant can be to be selected from vitamin E, vitamin C phosphoric ester, the ethoxyquinoline one or more.
When used enzyme is multiple in the invention described above method, can so that playing one's part to the full, it reach optimum efficiency according to its optimum temperature separately and the temperature and the acid-base value of acid-base value adjustment enzymolysis.The enzymolysis optimum condition of Partial Protein enzyme is: pepsin (vigor is 75000IU/G), pH1.5-2.5, temperature 40-60 ℃, hydrolysis 1-10 hour; Bacillus subtilis neutral proteinase (vigor is 50000IU/G), pH6.0-8.5, temperature 40-60 ℃, hydrolysis 1-10 hour, trypsase (vigor is 60000IU/G), pH7.0-9.5, temperature 40-60 ℃, hydrolysis 1-12 hour.Papain (600,000 IU/G) PH5.5-7.5, temperature 40-60 ℃, hydrolysis 1-12 hour.
Phagostimulant of the present invention, add in the feed according to the ratio of 1-10%wt, by contrast experiment, electrophysiology experiment and the growth experiment of ingesting, show that all this phagostimulant can successfully attract aquiculture animals such as the grouper feed of ingesting, improve food utilization efficiency effectively, promoted growth, strengthened disease resistance, improved survival rate, overcome the many problems in the current techniques, propagating artificially of aquiculture animals such as grouper had great importance and higher economic value.Therefore, phagostimulant of the present invention is the efficient phagostimulant that is suitable for multiple aquatic products economic animals such as grouper.
Efficient phagostimulant of the present invention has following advantage:
1. show that from the feed nutrient analysis result protein content is higher, ash is low, and more seminase hydrolysis products such as little peptide and free amine group acids etc. are arranged, and meets the demand of grouper son, young, juvenile fish nutrition substantially.
2. see tangible fresh and sweet flavor on the physical behavior, pure and fresh seafood fishy smell has been arranged in the sense of smell, can effectively stimulate the nervous system of fry to cause the reaction of ingesting; The color aspect is brighter, can well cause the vision response of lithosporic fry, has food calling effect preferably.
3. the raising experiment result through prelarva shows to have good food calling effect with efficient phagostimulant of the present invention, improves food utilization efficiency effectively, has promoted growth, has strengthened disease resistance, has improved survival rate, acts on very obvious.
The specific embodiment
Embodiment one: mussel meat 50kg and cod 30kg according to material water weight ratio=mix at 1: 5, are regulated pH9.0 and homogenate and carry out alkali extraction, the removal partial impurities of sieving; Add papain (600,000 IU/G) 1.2 grams, regulate suitable pH7.5,65 ℃ of constant temperature enzymolysis heated to 90 ℃ and keep 10 minutes so that enzyme deactivation after 4 hours in Cooling or heating jar, then lower the temperature back 45 ℃, add Aminopeptidase P and carboxypeptidase enzymolysis 5 hours again to eliminate bitter taste; Intact back adds the ethoxyquinoline that accounts for fresh animal protein sources raw material weight 0.8%, filter hydrolyzate, through concentrating, in Highspeedcentrifugingandsprayingdrier, carry out spray-drying and promptly obtain required animal protein enzymolysis thing dry powder then; Spray-dired shower nozzle rotating speed is 18000r/min, and inlet temperature is 180 ℃, and outlet temperature is 100 ℃.Animal protein enzymolysis thing dry powder with gained is absorption carrier, add the vitamin E of inosinicacid 3%, DMPT (dimethyl-β-DMPT) 1%, glutathione 5%, L-methionine 1%, betaine 10%, astaxanthin 2% and 0.5% in the gross weight ratio, through mixing, promptly obtaining required efficient phagostimulant after the drying.
The phagostimulant of above-mentioned gained added to by 5% raise grouper fry experiment in the feed, the time of having ingested from throwing something and feeding to is obviously short than the control group that does not add phagostimulant, growth and feed efficiency are all than control group high about 40%, survival rate also increases than control group, has got food calling and growth promotion effect preferably.
Embodiment two: blue or green squama fish 60kg and cuttlefish meat 10kg according to material water weight ratio=mix at 1: 4.5, are regulated pH9.0 and homogenate and carry out alkali extraction, the removal partial impurities of sieving; Add trypsase (6000IU/G) 1.0 gram and papain (700,000 IU/G) 0.8g, regulate suitable pH7.0,60 ℃ of constant temperature enzymolysis are after 3 hours in Cooling or heating jar, heat to 88 ℃ and keep 15 minutes so that enzyme deactivation, then lower the temperature back 60 ℃, add Aminopeptidase P enzymolysis 5 hours again to eliminate bitter taste; Intact back adds the ethoxyquinoline that accounts for fresh animal protein sources raw material weight 1.5%, filter hydrolyzate, through concentrating, in Highspeedcentrifugingandsprayingdrier, carry out spray-drying and promptly obtain required animal protein enzymolysis thing dry powder then; Spray-dired shower nozzle rotating speed is 16000r/min, and inlet temperature is 170 ℃, and outlet temperature is 950 ℃.Animal protein enzymolysis thing dry powder with gained is absorption carrier, add the ethoxyquinoline of adenosine diphosphate 4%, DMPT (dimethyl-β-DMPT) 0.8%, glutathione 5%, L-methionine 0.5%, L-alanine 0.5%, betaine 8%, astaxanthin 1.2% and 0.4% in the gross weight ratio, through mixing, promptly obtaining required efficient phagostimulant after the drying.
The phagostimulant of above-mentioned gained added to by 6% raise grouper juvenile fish experiment in the feed, the speed of ingesting is obviously fast than the control group that does not add phagostimulant, growth and feed efficiency are all than control group high about 35%, survival rate also increases than control group, has got food calling and growth promotion effect preferably.
Embodiment three: cuttlefish meat 30kg and cod 30kg according to material water weight ratio=mix at 1: 5, are regulated pH9.0 and homogenate and carry out alkali extraction, the removal partial impurities of sieving; Add papain (600,000 IU/G) 1.2 grams, regulate suitable pH7.5,65 ℃ of constant temperature enzymolysis heated to 90 ℃ and keep 10 minutes so that enzyme deactivation after 4 hours in Cooling or heating jar, then lower the temperature back 45 ℃, add Aminopeptidase P and carboxypeptidase enzymolysis 5 hours again to eliminate bitter taste; Intact back adds the ethoxyquinoline that accounts for fresh animal protein sources raw material weight 3%, filter hydrolyzate, through concentrating, in Highspeedcentrifugingandsprayingdrier, carry out spray-drying and promptly obtain required animal protein enzymolysis thing dry powder then; Spray-dired shower nozzle rotating speed is 18000r/min, and inlet temperature is 180 ℃, and outlet temperature is 100 ℃.Animal protein enzymolysis thing dry powder with gained is absorption carrier, add the vitamin E of inosinicacid 3%, DMPT (dimethyl-β-DMPT) 1%, glutathione 5%, L-methionine 1%, betaine 12%, astaxanthin 1.5% and 0.4% in the gross weight ratio, through mixing, promptly obtaining required efficient phagostimulant after the drying.
The phagostimulant of above-mentioned gained added to by 5% raise grouper fry experiment in the feed, the time of having ingested from throwing something and feeding to is obviously short than the control group that does not add phagostimulant, growth and feed efficiency are all than control group high about 42%, survival rate also increases than control group, has got food calling and growth promotion effect preferably.

Claims (6)

1. grouper phagostimulant, its one-tenth is grouped into and proportioning is by weight: animal protein enzymolysis thing dry powder 70-85%, nucleotides 2-10%, sulfur-containing compound 0.4-3%, compound amino acid 5-15%, betaine 2-20%, pigment 0.5-5% and antioxidant 0.1-0.5%; Described nucleotides is to be selected from inosinicacid, adenosine diphosphate, the adenosine triphosphate one or more; Described sulfur-containing compound is to be selected from dimethyl-β-DMPT, methyl-sulfoxide, the allicin one or more; Described compound amino acid is to be selected from glycine, taurine, alanine, glutamic acid, methionine, the acid of figured silk fabrics base, proline, arginine and the glutathione two or more; Described pigment is that astaxanthin is or/and vitamin B2; Described antioxidant is to be selected from vitamin E, vitamin C phosphoric ester, the ethoxyquinoline one or more.
2. according to the described phagostimulant of claim 1, it is characterized in that described animal protein enzymolysis thing dry powder is to add the antioxidant that accounts for fresh animal protein sources raw material weight 0.5-3% by fresh animal protein sources raw material behind protease hydrolyzed, spray-dried obtaining.
3. the preparation method of claim 1 or 2 described phagostimulants the steps include:
(1) with fresh animal protein source raw material, according to expecting water weight ratio=1: ratio (2-6) is mixed, and regulates pH8.5-9.5, and the alkali extraction is carried out in homogenate, crosses 60-100 mesh sieve removal partial impurities; Add protease, regulate 40-70 ℃ of temperature, and regulate suitable pH value according to the kind of used protease; 50-70 ℃ of constant temperature enzymolysis is 1-10 hour in Cooling or heating jar, heats to 80-95 ℃ then and keeps 10-15 minute so that enzyme deactivation is then lowered the temperature back 40-70 ℃, adds the exopeptidase enzymolysis again 1-10 hour; Intact back adds the antioxidant that accounts for fresh animal protein sources raw material weight 0.5-3%, filter hydrolyzate, through concentrating, spray-drying gets required animal protein enzymolysis thing dry powder then; Shower nozzle rotating speed during spray-drying is 8000-22000r/min, and inlet temperature is 150-230 ℃, and outlet temperature is 90-110 ℃; Described protease is one or more in trypsase, pepsin, chymotrypsin, papain, bromelain, the ficin; Exopeptidase is that Aminopeptidase P is or/and carboxypeptidase; The protease consumption is every gram bright protein sources raw material 5000-30000IU or every milliliter of enzymolysis liquid 50-500IU; The exopeptidase consumption is every gram bright protein sources raw material 5000-30000IU or every milliliter of enzymolysis liquid 50-500IU;
(2) the animal protein enzymolysis thing dry powder with gained is absorption carrier, adds nucleotides, sulfur-containing compound, compound amino acid, betaine, pigment and antioxidant in proportion, through mixing, promptly obtaining required phagostimulant after the drying.
4. in accordance with the method for claim 3, it is characterized in that the fresh animal protein source raw material described in the step (1) is to be selected from mussel meat, red worm, the seawater iced trash fish one or more.
5. in accordance with the method for claim 3, it is characterized in that the antioxidant described in the step (1) is to be selected from vitamin E, vitamin C phosphoric ester, the ethoxyquinoline one or more.
6. according to claim 3,4 or 5 described methods, the shower nozzle rotating speed when it is characterized in that spray-drying in the step (1) is 12000-20000r/min.
CNB2004100520445A 2004-11-04 2004-11-04 Grouper bait and method for making same Expired - Fee Related CN1281149C (en)

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CN100350853C (en) * 2005-09-02 2007-11-28 中国海洋大学 Phagostimulant of pseudosciaena crocea larvae
CN100459875C (en) * 2006-05-16 2009-02-11 华东师范大学 Phagostimulant specially for river crab and its prepn process
CN102018138A (en) * 2010-10-21 2011-04-20 江门粤海饲料有限公司 Freshwater aquaculture fish feed attractant growth-promoting additive
CN102067953A (en) * 2010-10-22 2011-05-25 广西粤海饲料有限公司 Feed promoting agent for seawater cultured fishes
CN102090537A (en) * 2010-12-27 2011-06-15 福建天马科技集团有限公司 Rockfish powdery compound feed
CN101513227B (en) * 2009-03-20 2011-10-19 中国水产科学研究院黑龙江水产研究所 Composite attraction agent for huchen
CN102293345A (en) * 2011-09-02 2011-12-28 汤阴县益农饲料有限责任公司 Method for preparing bait
CN102318784A (en) * 2011-10-24 2012-01-18 天津市晨辉饲料有限公司 Attractant for marine fish and preparation method for attractant
CN103431192A (en) * 2013-07-19 2013-12-11 清远海贝生物技术有限公司 Special feeding accelerant for Penaeus vannamei, and preparation method thereof
CN103478497A (en) * 2013-10-10 2014-01-01 山东升索鱼用饲料研究中心 Sea fish mixed feed for deepwater net cage culture and manufacturing method thereof
CN103989022A (en) * 2014-06-09 2014-08-20 中山大学 Phagostimulant specially used for hybrid snakeheads and preparation method thereof
CN104585484A (en) * 2015-01-23 2015-05-06 南宁市春华农业科技有限公司 Feed for controlling parotitis diseases of truogx sinensis and preparation method thereof
CN104799046A (en) * 2015-01-15 2015-07-29 南宁市春华农业科技有限公司 Truogx sinensis growth accelerator and preparation method thereof
CN104872420A (en) * 2015-05-19 2015-09-02 福建省淡水水产研究所 Premixed feed for epinephelussp
CN104920896A (en) * 2015-06-29 2015-09-23 溧阳正昌饲料科技有限公司 Novel aquatic product feeding attractant and preparation method thereof
CN107683981A (en) * 2017-10-23 2018-02-13 福建省农业科学院农业质量标准与检测技术研究所 A kind of aquatic feeds phagostimulant
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CN108271950A (en) * 2018-01-17 2018-07-13 广东海洋大学 A kind of feed addictive for promoting Grouper cultivating bottom of pond matter improvement water colour to improve
CN108432977A (en) * 2018-03-26 2018-08-24 佛山市顺德区活宝源生物科技有限公司 A kind of preparation and preparation method thereof promoting snakehead growth based on yeast class component
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Publication number Priority date Publication date Assignee Title
CN100350853C (en) * 2005-09-02 2007-11-28 中国海洋大学 Phagostimulant of pseudosciaena crocea larvae
CN100459875C (en) * 2006-05-16 2009-02-11 华东师范大学 Phagostimulant specially for river crab and its prepn process
CN101513227B (en) * 2009-03-20 2011-10-19 中国水产科学研究院黑龙江水产研究所 Composite attraction agent for huchen
CN102018138B (en) * 2010-10-21 2012-11-21 江门粤海饲料有限公司 Freshwater aquaculture fish feed attractant growth-promoting additive
CN102018138A (en) * 2010-10-21 2011-04-20 江门粤海饲料有限公司 Freshwater aquaculture fish feed attractant growth-promoting additive
CN102067953A (en) * 2010-10-22 2011-05-25 广西粤海饲料有限公司 Feed promoting agent for seawater cultured fishes
CN102090537A (en) * 2010-12-27 2011-06-15 福建天马科技集团有限公司 Rockfish powdery compound feed
CN102293345A (en) * 2011-09-02 2011-12-28 汤阴县益农饲料有限责任公司 Method for preparing bait
CN102293345B (en) * 2011-09-02 2013-01-23 汤阴县益农饲料有限责任公司 Method for preparing bait
CN102318784A (en) * 2011-10-24 2012-01-18 天津市晨辉饲料有限公司 Attractant for marine fish and preparation method for attractant
CN103431192A (en) * 2013-07-19 2013-12-11 清远海贝生物技术有限公司 Special feeding accelerant for Penaeus vannamei, and preparation method thereof
CN103478497A (en) * 2013-10-10 2014-01-01 山东升索鱼用饲料研究中心 Sea fish mixed feed for deepwater net cage culture and manufacturing method thereof
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CN103989022A (en) * 2014-06-09 2014-08-20 中山大学 Phagostimulant specially used for hybrid snakeheads and preparation method thereof
CN103989022B (en) * 2014-06-09 2016-02-24 中山大学 A kind of hybridized snakehead fish phagostimulant specially and preparation method thereof
CN104799046A (en) * 2015-01-15 2015-07-29 南宁市春华农业科技有限公司 Truogx sinensis growth accelerator and preparation method thereof
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CN108077648A (en) * 2017-12-14 2018-05-29 柳州市柳科科技有限公司 A kind of high protein feed for improving fish grazing rate and preparation method thereof
CN108142734A (en) * 2018-01-04 2018-06-12 广东越群海洋生物研究开发有限公司 A kind of east star spot opening material and preparation method thereof
CN108271950A (en) * 2018-01-17 2018-07-13 广东海洋大学 A kind of feed addictive for promoting Grouper cultivating bottom of pond matter improvement water colour to improve
CN108432977A (en) * 2018-03-26 2018-08-24 佛山市顺德区活宝源生物科技有限公司 A kind of preparation and preparation method thereof promoting snakehead growth based on yeast class component
CN109007255A (en) * 2018-07-27 2018-12-18 普定县冠辉种养殖专业合作社 A kind of beef cattle forage phagostimulant and preparation method thereof for promoting to digest and assimilate
CN109452496A (en) * 2018-12-29 2019-03-12 盐城工学院 A kind of aquaculture phagostimulant and preparation method thereof
CN110604313A (en) * 2019-09-30 2019-12-24 浙江海洋大学 Method for preparing high-protein high-calcium supplement by using mussel waste materials

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