CN1602721A - Cow milk promoting formulation and its preparation method - Google Patents

Abstract

This invention provides a kind of preparation for increasing milk of milk cow. The preparation is made of by weight: dangshen10-20, shuangong4-10, danggui4-10, wangbuliuxing6-8, liulutong5-15, tongcao4-10, huangshi15-25, loulu6-15, kuizi10-20, blowball0-20, balloonflower1-8, pangolin0-15, yellow bean0-15. Way to produce: a. mix dangshen, wangbuliu and yellow bean, then crush them and sift; b. crush pangolin to the powder and sift; c. fry dongkuizi before mixed; d. water fry other materials; filtrate the three kinds of filtrate; e. mix an and d, add b and c, mix them, then it's made over.

Description

Promote preparation of fodder for increasing milk and preparation method thereof

Technical field

The present invention relates to a kind of health medicine that is used for milk cow, especially a kind of is the preparation that raw material is made with the Chinese herbal medicine, the invention still further relates to the preparation method of said preparation.

Background technology

Improving the milk production of cow, increase economic benefit, is the problem that cattle farm and individual cowboying family extremely are concerned about.At present, herding production is in order to reach the desirable newborn benefit that increases, and the feed addictive on the market is of a great variety.More or less there is residue problem in some feed addictive in carcass and products thereof milk, this residue constitutes potential danger to eater's health to some extent, makes its application be subjected to certain limitation.And the Chinese herbal medicine in motherland's medical treasure-house can be avoided the deficiency of above-mentioned additive, has unique advantages.Chinese herbal medicine can be traced back to the record of " the Chinese catalpa leaf is raised pig, loose three times " in the medicine book Shennong's Herbal the earliest as feed addictive, in the Western Han Dynastry of China " the fertile pig method of numb salt " is just arranged the epoch.Facts have proved that Chinese herbal medicine has is nontoxic, harmless, do not have advantage such as harmful medicament residue.

Chinese herbal and crude drugs preparations is used for fodder for increasing milk, the existing report of lactogenesis, as usefulness Radix Codonopsis, Radix Angelicae Sinensis etc. such as Xie Huisheng 10 flavor Chinese prescriptions " increasing breast looses " by name, and referring to " Chinese veterinarian's medical magazine " 2:6 such as Xie Huisheng, 1994.And for example the Chen Hui of Shandong Laiyang Agricultural College cloud with the seed of cowherb, draw together that beach wormwood, the Radix Astragali,  hold, dandelion, Divine Comedy, anise, eight kinds of medicament composing prescriptions of sodium acid carbonate " increasing breast looses ", referring to Chen Huiyun " Chinese veterinarian's medical magazine " 4:9,1995.Li Xichou (1999) makes feed additive for promoting milk secretion of milk cow with the seed of cowherb, LIULUTONG, the stem pith of the rice-paper plant, akebi, pangolin, Radix Rhapontici seu Radix Echinopsis, luffa, likes ugly feed additive for promoting milk secretion of milk cow [P] 1999:99109394.1 referring to Lee.Zhang Faliang etc. form prescription " No. 2, yellowish-white drink " with 7 flavor Chinese medicines such as the Radix Astragali, the root of herbaceous peony, Radix Glycyrrhizae, referring to " ox magazines 24 (5): 59,1998 such as Zhang Faliang.

The powder of fodder for increasing milk and additive are most just simply to mix pulverizing with Chinese herbal medicine in proportion, makes coarsely, and taste of traditional Chinese medicine is very dense.Liquid increasing milk agent then palatability is poorer, influences feeding and the normal appetite of milk cow.

Summary of the invention

One of purpose of the present invention provides a kind of materials such as any chemicals, hormone, antibiotic that do not contain, it is remarkable to increase newborn effect, palatability is good, nontoxic, without any side effects, can improve the milk cow immunity of organisms, increase appetite, effectively prevent the preparation of the promotion fodder for increasing milk of various diseases perinatal period; Two of purpose provides the preparation method that the production technology that realizes one of purpose simply promotes the preparation of fodder for increasing milk.

One of purpose of the present invention can realize by following technical measures

The preparation of promotion fodder for increasing milk of the present invention is the preparation of being made by following weight parts proportioning herbal raw material

Radix Codonopsis 10～20 Ligusticum wallichiis 4～10 Radix Angelicae Sinensis 4～10

The seed of cowherb 6～18 LIULUTONGs 5～15 stem piths of the rice-paper plant 4～10

The Radix Astragali 15～25 Radix Rhapontici seu Radix Echinopsis 6～15 chingma abutilon seeds 10～20

Dandelion 0～20 balloonflower root 1～8 pangolin 0～15

Stir-bake to yellowish 0～15

One of purpose of the present invention also can realize by following technical measures

Wherein preferred weight part proportioning of each raw material is

Radix Codonopsis 15 Ligusticum wallichiis 8 Radix Angelicae Sinensis 8

The seed of cowherb 10 LIULUTONGs 10 stem piths of the rice-paper plant 8

The Radix Astragali 20 Radix Rhapontici seu Radix Echinopsis 10 chingma abutilon seeds 15

Balloonflower root 5

The combination 10 that dandelion or pangolin or stir-bake to yellowish beans or this three component are any two kinds

Two of purpose of the present invention can realize by following technical measures

The preparation method of the preparation of promotion fodder for increasing milk of the present invention respectively will

A, Radix Codonopsis, the seed of cowherb and stir-bake to yellowish beans mix the back by proportioning to be pulverized, and sieves;

B, pangolin are worn into fine powder, sieve;

The frying before mixing of c, chingma abutilon seed; Then will

D, all the other raw material decoctings 3 times filter, merge filtrate, concentrated respectively three times; At last

E, a and d are merged, add b and c again, be processed into preparation behind the stirring and evenly mixing.

Two of purpose of the present invention also can realize by following technical measures

The described fineness of sieving is 40～80 orders; 1st, 2,3 decoctings add 10,8,8 times of water gagings successively respectively, respectively fry in shallow oil 1 hour.

Concrete preparation process is as follows:

1. measure Radix Codonopsis, the seed of cowherb and stir-bake to yellowish beans by component, be broken into fine powder, cross the 40-80 mesh sieve;

2. measure pangolin by component, wear into fine powder, cross 80 mesh sieves;

3. before mixing chingma abutilon seed is fried;

4. remaining herbal raw material is added 10 times of water gagings and fry in shallow oil 1h, for the second time and add 8 times of water gagings respectively for the third time and respectively fry in shallow oil 1h, filter, merge three times decocting liquid, be concentrated into the 1/50-1/90 of original volume;

5. mix 1 and 4, add 2 and 3, abundant stirring and evenly mixing, 50-70 ℃ of oven dry, packing, sterilization gets pulvis.Or be pressed into the particle that diameter is 3-6mm after stirring, and 50-70 ℃ of oven dry, packing, sterilization gets granule.

The present invention has overcome the deficiency of above Chinese prescription, is guidance with the traditional Chinese medicine theory, the theoretical and nutrition prescription of utilization Chinese veterinarian; develop the pure tcm product of fodder for increasing milk granule, do not contain materials such as any chemicals, hormone, antibiotic, have benefiting qi and nourishing blood; clearing and activating the channels and collaterals, dredge newborn road, mammary gland, the lactogenesis detumescence; the benefit essence protects the liver, and coordinating Chong and Conception Vessels is aid digestion; short appetite; and can improve the milk cow immunity of organisms, replenish its nutrition, and can effectively prevent function such as various diseases perinatal period.Said preparation increases that newborn effect is remarkable, and palatability is good, and is nontoxic, without any side effects, and cheap, low price, and production technology is simple, and economic benefit is obvious, is suitable for wide popularization and application in animal husbandry.

The principle of fodder for increasing milk:

Chinese veterinarian thinks that " breast forms for the qi and blood life.Blood is wet nurse, and no blood then breast is difficult to give birth to; Gas is that breast is handsome, and no gas then breast is difficult down.The breast row is complete bad pneumatic, and lid is changed life with promoting circulation of blood ".Milk cow has gas to lose resembling of the deficiency of blood or qi depression to blood stasis postpartum more, thus the medicine that adopts benefiting qi and nourishing blood, clearing and activating the channels and collaterals, the promoting the circulation of qi of regulating the flow of vital energy in conjunction with " real when dredge it, empty when benefits, dredge mend and with " theoretical prescription, to increase breast, logical breast, change newborn, protect the liver remarkable result all arranged.

By monarch, minister, help, make the principles of formulating prescriptions to analyze prescription compatibility of medicines:

Pangolin flavor is salty, and cold nature is good at walking to scurry, and property specially row is loose, and can stimulate the menstrual flow network and reaches disease institute.Qualcomm meridian, following milk is the key medicine of the lactogenesis of stimulating the menstrual flow.Radix Astragali flavor is sweet, slightly warm in nature, gas that can the tonifying spleen lung has the merit of elevate a turnable ladder yang-energy, with pangolin be we's monarch drug in a prescription altogether.Radix Codonopsis tonifying spleen green blood, nourishing generate fluid; Seed of cowherb hardship, flat can be walked blood system, property row and incessantly also, merit is arrogated to oneself the activating blood to promote menstruation lactogenesis; Sweet light being slightly cold of the stem pith of the rice-paper plant gone into bright stomach warp, reaches in the ventilation and following milk, and the logical breast of effect clearing away heat and promoting diuresis does not stay same usefulness with pangolin, king, and effect is better, this medicine with Radix Codonopsis, Wang Buliu, playing both sides of the street is we's ministerial drug altogether.Radix Angelicae Sinensis is sweet, hot, warm, enriches blood and invigorates blood circulation; Radix Rhapontici seu Radix Echinopsis hardship, cold; Chingma abutilon seed is clearing heat and detoxicating, following milk, with Radix Angelicae Sinensis be adjutant altogether.Up being of balloonflower root medicine carrying makes medicine.Take a broad view of full side, benefiting qi and nourishing blood, nourishing Yin and promoting production of body fluid, qualcomm meridian, following milk amounts to the effect that newborn rich milk gushes.

We's raw materials used (medicinal material) standard meets 5 editions one one of Chinese Pharmacopoeia.

The Radix Astragali: be the dry root of legume astragalus mongolicus Asthagalus membrana cues (Fish.) Bge.Uar.Mongholicus (Bge.) Hsiao or Astragalus membranacus Asthagalus membranaceueus (Fisch.) Bge..Contain Astragaloside IV and must not be less than 0.06%.Contain saponin component in the Radix Astragali, wherein Astragaloside IV is one of its main member, contains compositions such as polysaccharide, flavonoids and amino acid, trace element, cupreol in addition.

Pangolin (system) (Squama manitis): this product is the scale and shell of Manidae animal pangolin Manis penadactyla Linnaeus.Contain several amino acids and trace element, it is loose to concoct the back quality, is easy to pulverize.

The seed of cowherb (stir-fry) (Semen vaccariae): this product is the dry mature seed of pinkwort cow-fat Vaccaria segetalis (Neck) Garcke.Contain multiple saponin, wherein vacsedoside is made up of gypsogenin, glucuronic acid, glucose, wood sugar, arabinose, salt soap sugar, rhamnose.Starch-containing in addition 5.3%, fat 4.32%, protein 9.34%, ash content 4.28%.After frying, its active ingredient is fried easily.

The stem pith of the rice-paper plant (Medulla tetrapanacis): this product is the dry stem marrow of Araliaceae rice-paper plant Tetrapanax Papyriferus (Hook) k.koch.Contain ash content 5.95%, fat 1.07%, protein 1.11%, crude fibre 48.73%, pentosan 5.83%.Still contain uronic acid 28.04%, wherein a part is present in poly-β-D galacturonic acid etc.

Radix Rhapontici seu Radix Echinopsis (Radix rhapontici): this product is the dry root that feverfew is prayed state Radix Rhapontici seu Radix Echinopsis Rhaponticum uniflorum (L.) DC..Contain ecdysterone and a small amount of volatile oil, modern pharmacology experimental results show that the sterol of casting off a skin that extracts in the Radix Rhapontici seu Radix Echinopsis has the immunocompetent effect of the mouse cell of raising.The decocting thing of Radix Rhapontici seu Radix Echinopsis root and aerial part has antioxidation.

Radix Codonopsis: this product is Campanulaceae herbaceos perennial Radix Codonopsis Codonopsis Pilosula (Franch.) Nannf. and the root that belongs to various plants together.The property sweet, flat, return spleen, lung channel.Contain saponin in the Radix Codonopsis, protein, vitamin B1, B2, sucrose, synanthrin, alkaloid etc.Nervous system there is excitation, can strengthens the phagocytic function of reticuloendothelial system, improve body resistance against diseases.Can expand peripheral vessels and inhibition adrenaline and be hypotensive effect.The white blood cell that causes for chemotherapy and radiotherapy descends the effect that makes its rising is arranged.Radix Codonopsis uses and deficiency of vital energy deficiency, mainly is the illness due to spleen-lung Qi deficiency.Again as the benefiting qi and nourishing blood medicine.Experimental results show that this product can the effect by spleen increase red blood cell and hemochrome, be applicable to the illness of the sallow and qi-blood deficiency that the chronic bleeding illness causes of the deficiency of blood.

Ligusticum wallichii: this product is the rhizome of Umbelliferae herbaceos perennial Ligusticum wallichii Ligusticum wallichii Franch..The property suffering, temperature is returned liver, courage, pericardium channel.Effect with blood-activating and qi-promoting, wind-expelling pain-stopping.Contain volatile oil in the Ligusticum wallichii, the derivative of a lactones body is arranged in the oil, contain oily alkaloid, forulic acid etc. in addition.Now isolated tetramethylpyrazine (ligustrazine).Rhizoma Chuanxiong extractum (10%) can make it shrink and strengthen the tame rabbit uterus of stripped gestation.Then benumb in a large number and shrink and stop.Effects such as analgesia, calmness, antispastic are arranged.The excited medullary respiratory center of energy, vasomotor center are directly expanded peripheral vessels, coronary blood flow are increased and the increase of lower extremity blood flow amount, bring high blood pressure down.In vitro test all has inhibitory action to sonne bacillus, Escherichia coli, typhoid bacillus, Pseudomonas aeruginosa, comma bacillus and various skin fungi.In addition, the effect that also has antivitamin E to lack.It is reported that Ligusticum wallichii is used for high blood pressure, share that good synergy is arranged with reserpine.Tetramethylpyrazine (ligustrazine) has hemangiectasis, increases coronary blood flow, improves effects such as microcirculation and inhibition platelet aggregation, and can pass through blood-brain barrier.

Dandelion: this product is the whole herb with root of composite family herbaceos perennial dandelion Taraxacum mongolicum Hand.-Mazz. and multiple congener thereof.The property hardship, sweet, cold.Return liver, stomach warp.Contain taraxasterol, taraxacin, taraxacin, synanthrin, pectin, choline etc.Dandelion has stronger bactericidal action to staphylococcus aureus, hemolytic streptococcus.Diplococcus pneumopniae, meningococcus, corynebacterium diphtheriae, Pseudomonas aeruginosa, shigella dysenteriae, typhoid bacillus etc. also there are certain bactericidal action, effects such as cholagogic diuresis are still arranged.

Chingma abutilon seed: this product is the mature seed of the annual or herbaceos perennial winter certain herbaceous plants with big flowers Malva verticillata L. of Malvaceae.The property sweet, cold, return large intestine, small intestinl channel.Have effects such as inducing diuresis for treating strangurtia, lactogenesis, ease constipation.

LIULUTONG (playing both sides of the street): this product is Hamamelidaceae deciduous tree Chinese sweet gum Liquidambar taiwaniana Hance, the fruit of epithmut., and it is real to have another name called maple.The property suffering, hardship, flat.Return liver, stomach, urinary bladder channel.Effect with wet, the network that stimulates the menstrual flow of dispeling the wind, Li Shui, lactogenesis.Contain volatile oil, wherein be mainly sesquiterpene compounds and cinnamic acid ester, cinnamic acid, cinnamic alcohol, left-handed borneol.Be usually used in stagnation of QI-blood, alactation, swollen breasts, can with compatibilities such as the rascal of the promoting flow of qi and blood circulation, pangolin.

Radix Angelicae Sinensis (Radix angeliacae sinensis): this product is umbelliferae angelica Angeliaca sinensis (the dry root of Oliv.Diels.Mainly contain volatile oil and water soluble ingredient.Seed amino acid etc. is arranged in the water soluble ingredient surplus forulic acid, succinic acid, nicotinic acid, uracil, gland pyrimidine, the sucrose and 10, and wherein forulic acid has the effect of the platelet aggregation of inhibition.

Balloonflower root (Radix ophiopogonis): this product is the dry root of Campanulaceae Platycodon grandiflouorum Platycodon grandiflorum (Jacq.) A.Dck..Contain saponin, polygalic acid, platycodigenin; Also contain spinasterol, hitodesterol-β-D glycoside, betulin, and contain synanthrin, platycodonin, platycogenic acid A, B, C.

According to the physicochemical property of contained active ingredient of component drug and main component, with reference to same medicine process conditions and traditional preparation method in other modern preparations, carried out craft screening than system, determined the preparation technology of this granule.For more valuable medicine pangolin,, adopt the pulverizing back directly to add for fully keeping the active ingredient of this medicine.For the denseer Ligusticum wallichii of taste of traditional Chinese medicine, Radix Angelicae Sinensis, LIULUTONG, the stem pith of the rice-paper plant, the Radix Astragali, Radix Rhapontici seu Radix Echinopsis, dandelion, balloonflower root etc. eight flavor Chinese medicines, the principle according to " mix fry in shallow oil compatible " adopts water extraction to extract the active principle of each medicine.Radix Codonopsis, seed of cowherb flavor be sweetness, does not contain taste of traditional Chinese medicine, carries out seasoning so employing grinds direct adding jointly with the stir-bake to yellowish beans.The chingma abutilon seed flavor is fragrant, and harder, and fragrance is denseer after frying, for avoiding pulverizing the mode of directly adding after the lost employing frying of back fragrance.After adding the fine powder and chingma abutilon seed of Radix Codonopsis, the seed of cowherb, stir-bake to yellowish beans, covered the bitter taste of decocting herb concentrate effectively, made the particle flavor that makes fragrant, palatability is fabulous.

Increasing the relevant leading indicator of breast measures:

1, grouping is observed in experiment

Require: the milk cow of calving just, determine kind, age, body weight, parity, lactation fate, former lactation amount.By age, the strict pairing of principle of parity, output of milk basically identical, be divided into test group and control group then at random, 30 every group, totally 60.

2, feeding and management

Feed 3 every day in the equal holddown groove of experiment milk cow position.Test group is identical with the control group daily ration.Test group only adds in fine fodder at noon and increases newborn granule, first three day addings side one, later every day side of taking two or side three or square four 60g.

3, experimental period:

Experimental period is five months, and to be advisable spring, environment temperature is 15 ℃ of the bests.Because milk bovine lactation optimum gas temperature generally is at 10-15 ℃, high temperature makes the disorder of milk cow organism endocrine, directly influences the decline of the output of milk.So be advisable for environment temperature 10-15 ℃.

4, the leading indicator of measuring

4.1 the output of milk

Milk every day two to three times, the output of milk of the accurate every cow head of weighing, and carry out record, the record content comprises: adopt phase, fate, the output of milk, and carry out the different statistics of probability difference.(the results are shown in Table 1)

As can be seen from Table 1: test group output of milk in five months experimental periods all is higher than control group, wherein, 1-2 month test group output of milk improves extremely significantly (P＜0.01) after taking increasing milk agent, and 3-5 month test group output of milk is also apparently higher than control group (P＜0.05).The every cow head daily milk yield of test group average specific control group improves 4.50Kg in experimental period.

Table 1: each monthly average output of milk unit of test group and control group: head, Kg,

Group	Average day of the equal daily yielding of sample number parity head
			May April in March February in January the output of milk
Test group control group difference	???30???3.10±1.46??28.54???28.37???28.33???27.99???28.26??????28.29 ???30???3.20±1.28??23.50???23.66???23.92???23.89???24.02??????23.79 ????????????????????5.04????4.71????4.41????4.10????4.24???????4.50

4.2 the detection of milk composition

(1) sampling:

Getting each 2 parts in the milk cow's milk sample of participating in the experiment on the the 1st, 3,5,50,90 day in experiment.The method of sampling: gather before filter the back of milking in the morning.Pour into and stir evenly in the clean milk churn squeezing good normal milk, at the bottom of then suction pipe from top to bottom slowly being inserted to barrel, milk liquid is flowed in the suction pipe naturally.Get 3-4 time so repeatedly, adopt the sample breast of required mensuration.

(2) assay method:

The a centrifugal degreasing of newborn sample is adopted by institute, adds 22% perchloric acid by 9: 1, and fully effect back protein precipitation by centrifugation matter is neutralized to pH7-8 with 2.2mol/L KOH solution then, and the centrifuging and taking supernatant is stored in 0-4 ℃ of refrigerator, cAMP to be measured.CAMP measured by radioimmunoassay, instrument are FJ-2100 type liquid scintillation counter.The newborn sample of another part is put in the 1-5 ℃ of ice can and is preserved, and inferior daily dairy products comprehensive tester MTLKOSCAN-104 (production of Denmark Foss electric corporation) measures, contrast statistics then.Testing index has: butterfat percnetage, lactose, lactoprotein, dry, proportion.The results are shown in Table 2, table 3

Table 2: increasing milk agent is to the unit that influences of milk composition: %

Group butterfat percnetage lactose milk protein as dry matter proportion
	Control group 3.56+0.44 4.81+0.26 3.07+0.29 11.44+0.91 1.02+0.12 test group 3.87+0.53 4.79+0.33 3.11+0.33 11.21+1.10 1.03+0.11

Table 3: the content unit of Ruzhong cAMP: pmol/ml

Fate cAMP content, (d) test group (n=30) control group (n=30
	??1?????189.56±135.80?????123.19±210.33 ??3?????635.78±301.45?????326.35±289.65 ??5?????615.26±345.76?????512.36±395.21 ??50????1069.68±589.23????789.61±578.26 ??90????1278.64±895.46????916.28±678.39

As can be seen from Table 2, the test group butterfat percnetage improves 8.7% than control group, significant difference (P＜0.05), and lactoprotein increases by 1.3% than control group.And lactose, dry, content and two groups of basically identicals of proportion.Illustrate that granule of the present invention can improve butterfat percnetage and content of milk protein, improve the milk quality, improve nutritive value.

Once reported that the output of milk rose with the rising of Ruzhong cAMP content in the past experiment, during the cAMP peaking, the output of milk is peaking also.As can be seen from Table 2, test group cAMP content is higher than control group.

Lactation is the biological process that many hormones participate in the complexity of adjusting.Hormones such as prolactin, auxin, thyroxine lactation start and keep in play main regulating and controlling effect, these effects all realize by its second messenger cAMP, cGMP.Therefore the height of cAMP, cGMP level has very big influence to the lactation activity in the mammary glandular cell.Studies show that cAMP can impel the decomposition of materials such as fat, glycogen, mobilize body fat to stock and supply raw materials to milk is synthetic.And cGMP can impel the synthetic and activation of zymoprotein relevant with lactation in the cell, and by the effect of enzyme the synthetic metabolic activity of milk is strengthened.The cAMP level is high more, and the raw material of synthetic breast is just abundant more, and the output of milk is just high more.Ruzhong cAMP content raises, and provides the raw material of synthesise lactic to increase, and lactoprotein, butterfat is synthetic increases.

We have added some Qi-tonifying drug (as the Radix Astragali, Radix Codonopsis) and blood circulation and stasis-eliminating medicine (as Ligusticum wallichii, the seed of cowherb) in prescription, and on theory of traditional Chinese medical science, Qi-tonifying drug has the effect of kidney tonifying gas, beneficial temper.The spleen being the foundation of acquired constitution, the source of vital function; The lung is concerned with QI of the whole body, so the gas of spleen lung is vigorous, various metabolic activities are strengthened thereupon.Drug for invigorating blood circulation and eliminating stasis has promoting blood circulation, and the mediation blood vessels are strengthened sanguimotor effect.The test group milk cow showed honey stomach after use increased newborn granule, and milk performance strengthens, and the output of milk is significantly higher than control group.Qi-tonifying drug has the effect of similar cortex hormone of aadrenaline, can strengthen sympathetic-adrenal medella activity, and the cAMP level is raise; The active ingredient ligustrazine of drug for invigorating blood circulation and eliminating stasis Ligusticum wallichii can obviously suppress the activity of di-phosphate ester, reduces the degraded of cAMP, thereby the cAMP level is raise.

As can be seen from Table 3, test group Ruzhong cAMP content is higher than control group, so wherein lactoprotein, dairy fat content are significantly higher than control group (P＜0.05).Therefore, fodder for increasing milk granule of the present invention can obviously improve milk-quality, improves nutritive value.

4.3 the mensuration of blood parameters

The foreneck venous blood collection of feeding morning was advisable on the 4th day, and all the other times can be in the in-test stochastical sampling.Adopt blood sample add anticoagulant heparin, 37 ℃ leave standstill 0.5h after, centrifugal 5min (3000r/min) makes blood plasma, the peace bottle of packing into is sealed, it is to be determined to put ultra low temperature freezer.

(1) prolactin (PRL) is measured: with two anti-radioimmunologies

(2) estradiol (E2) is measured: with two anti-radioimmunologies

(3) blood sugar (G) is measured: with glucose oxidase end point analysis method

(4) serum paraoxonase is measured: ferrous sulfate phosphorus molybdenum blue colorimetric method

(5) serum calcium is measured: EDTA method or orthocresol phthalein complexon colorimetric method

(6) serum glutamic oxalacetic transaminase (GOT) is measured: Lai Shi colorimetric method or improvement Ka Menshi method

(7) serum alkaline phosphatase (ALP) is measured: to nitro disodium hydrogen phosphate-diethanolamine method

(8) serum (γ-GT) measure: gamma-glutamyl paranitroanilinum matrix method

(9) total serum protein (TP), albumin (Alb): biuret colorimetric method and bromocresol green colorimetric method

(10) serum ketoboidies: salicylide colorimetric method

(11) serum triglyceride (TG), T-CHOL (CT): isopropyl alcohol extracting acetylacetone,2,4-pentanedione development process and isopropyl alcohol extracting high ferro-acetic acid-sulfuric acid development process

The results are shown in Table 4～6

Illustrate:

1) milk cow E2 in postpartum descends, and PRL raises, and to increase lactation amount, removes the inhibitory action of E2 to lactation.PRL raises and E2 descends indicates that lactation increases.Postpartum these two kinds of hormones variation, especially PRL all can reach peak at every turn, and is extremely favourable to lactation.PRL makes the synthetic increase of RNA in the mammary glandular cell, thereby make the synthetic acceleration of important substance such as interior relevant enzyme of cell and protein, show preceding the starting of lactation to act on, PRL also can induce the cAMP activated protein kinase, thereby make glycogen and lipolysis, increase the raw material of nutrition of breast milk material.PRL level and lactation amount are proportionate.

2) perinatal period milk cow GOT, γ-GT the activity arrival of containing the phase along with the arriving and the lactation in term continue to rise, Alb continues to descend, and has reflected that perinatal period, the milk cow liver function was subjected to grievous injury.Because a large amount of mobilization that the arrival of phase causes storage fat in the body are contained in lactation, intermediate product is stored up in vivo, and hepatic glycogen consumes in a large number, makes the aggravation of liver load.In addition, body fat is mobilized and is caused liver fatty infiltration, and liver parenchyma and liver function suffer damage.Its result is because the metabolism utilization of fat metabolic intermediate ketoboidies weakens, hepatic parenchymal steatosis etc. and bring out many perinatal period diseases.Though test group milk cow GOT, γ-GT are in rising trend, enzyme activity significantly is lower than control group.Illustrate that increasing milk agent normally has good effect to the milk cow liver function.

3) ALP sharply descended in postpartum, and the variation of ALP is also closely related with liver function.Test group milk cow ALP significantly is lower than control group (P＜0.05), shows that this product has the good action that improves or safeguard liver function.

4) postpartum, blood ketone, blood lipid level rose, and blood sugar descends.Blood fat, blood ketone level rise and certainly will cause the raising of ketoacidosis, fatty liver, acid poisoning and other perinatal period disease incidences.Therefore, slow down body fat and mobilize, safeguard the normal function of hepatic metabolism, it is favourable to protecting the liver to increase blood-sugar content.Result of the test shows that test group milk ox blood ketone content and content of triglyceride are low than control group, and blood-sugar content is kept higher level.Illustrate that this product is slowing down the mobilization of body fat, increase blood bupropion metabolite is kept milk cow energetic supersession aspect good effect.

Table 4: hormone serum level measured value

Fate E2 content (pg/ml) PRL content (mg/ml) is test group (n=30) control group (n=30) test group (n=30) control group (n=30) (d)
	??0??????1450.4±234.5???1478±356??????????278±34?????????283±26 ??1??????214.4±103.1????351.7±78.8????????199±36?????????168±45 ??4??????12.3±1.4???????13.64±3.4?????????156±27?????????120±39 ??50?????12.9±3.6???????13.5±4.2??????????68±24??????????139±19 ??90?????12.1±1.3???????14.6±2.7??????????174±28?????????142±27

Table 5: sero-enzyme index determining value

Fate GOT (ku/L) ALP (iu/L) γ-GT (u/L) is control group test group control group test group control group test group (d)
	?0???102.2±35.3??100.7±27.1??374.7±90.1??348.3±105.4??23.6±6.3????21.1±4.4 ?1???164.6±36.1??121.2±31.2??350.7±87.3??310.5±114.3??27.8±8.1????24.3±5.6 ?4???175.8±40.1??132.4±45.2??339.1±96.2??298.4±78.2???30.2±7.2????26.3±6.2 ?50??188.5±50.6??156.8±47.2??310.4±87.9??278.1±87.1???36.7±5.9????30.5±7.6 ?90??199.1±62.3??160.7±40.6??291.5±75.3??254.1±88.3???39.2±6.1????32.4±6.9

Table 6: serum sugar, fat, albumen, ketoboidies, serum calcium, serum paraoxonase measurement result

Group project fate 014 50

( mg/100ml ) 62.4±7.8 56.3±10.7 49.7±12.4 38.1±14.6 64.6±9.4 60.2±12.3 54.4±13.7 45.2±11.2 ( mg/100ml ) 14.8±6.3 24.5±15.3 27.7±18.9 36.2±14.31 11.52±7.4 12.5±3.8 12.9±4.1 18.3±10.2 188.8±55.2 190.2±21.2 195.3±29.6 198.9±30. ( mg/100ml ) 173.1±13.8 191.2±51.3 196.5±60.7 199.8±45.6 264.1±53.1 289.3±15.2 303.7±19.8 380.8±56.8 ( mg/100ml ) 248.9±41.6 264.3±28.1 285.4±49.0 298.2±44.8 ( g/100ml ) 7.1±0.5 7.2±0.6 7.3±0.5 7.3±0.8 7.6±0.5 7.8±0.6 7.8±0.7 7.9±0.5 ( g/100ml ) 3.8±0.3 3.7±0.4 3.6±0.4 3.5±0.6 4.2±0.6 3.9±0.5 3.8±0.4 3.8±0.7 5.24±1.17 4.87±0.98 4.26±0.91 4.10±0.78 ( mg/100ml ) 4.89±0.56 4.78±0.76 4.76±0.78 4.49±1.02 8.79±1.13 8.56±0.58 8.48±0.74 8.38±0.60 ( mg/100ml ) 8.76±0.76 8.70±0.86 8.88±0.84 8.94±0.76

4.4 pathological observation statistics

Test group and control group milk cow are carried out pathological observation, observation index: do not eat in mastitis, retention of afterbirth, pregnancy general edema, postpartum paralysis, postpartum ketoacidosis, postpartum, lochiorrhagia.(the results are shown in Table 7)

As can be seen from Table 7, this product increases that newborn granule can not eaten several diseases of milk cow perinatal period such as retention of afterbirth, pregnancy general edema, postpartum paralysis, postpartum ketoacidosis, postpartum, lochiorrhagia etc. plays certain prevention effect.This safeguards the normal of milk cow liver function with it, slow down body fat and mobilize, and it is closely related to keep the clinical pharmacology effect that Chinese medicine had in milk cow eubolism and the prescription.

Mammitis is a kind of disease that milk cow is the most general, harm is maximum, therefore, the prevention of mammitis is had the meaning of special Chinese medicine on the milk farm.The Qi-tonifying drug Radix Astragali in the prescription etc. contains multiple bioactive ingredients, energy activated T cell, the activity of enhancing macrophage and NK.Can also act on DNA and cyclic adenosine monophosphate system (cAMP/cGMP) in addition, improve the DNA synthetic ratio, promote the generation of immune substances such as complement, antibody, interferon, strengthen body's immunity, thereby improve body resistance against diseases.Dandelion has stronger bactericidal action to staphylococcus aureus, hemolytic streptococcus.Diplococcus pneumopniae, meningococcus, corynebacterium diphtheriae, Pseudomonas aeruginosa, shigella dysenteriae, typhoid bacillus etc. also there are certain bactericidal action, the prevention mastitis for milk cows is played a role.In process of the test, the control group mammitis incidence of disease reaches 20%, and none example of test group takes place, and as seen the agent of this product fodder for increasing milk strengthens premunition to improving the milk cow immunity of organisms, prevents certain effect that has of mammitis.

Table 7: clinical onset situation unit: head

Group mastitis retention of afterbirth pregnancy general edema postpartum paralysis ketoacidosis in postpartum is not eaten lochiorrhagia postpartum
	Control group 6 12 99 25 16 20 test group 0823 13 6 14

Annotate: the above person of (1) blood acetone number rising 10mg/100ml is judged to be the ketoacidosis ox;

(2) postpartum aphagia, apocleisis fine fodder, ensiling appear with visiting, feed intake acutely drops to standard;

(3) still to efflux a large amount of rheuminess things from vagina with one week of postpartum be criterion to lochiorrhagia.

The specific embodiment

Embodiment 1: component is as follows, is weight portion:

Radix Codonopsis 14 Ligusticum wallichiis 7 Radix Angelicae Sinensis 7 seeds of cowherb 12 LIULUTONGs 9 stem piths of the rice-paper plant 7

The Radix Astragali 16 Radix Rhapontici seu Radix Echinopsis 9 chingma abutilon seeds 15 balloonflower roots 6 pangolins 9

One, the pre-treatment of bulk drug

1, pulverizing medicinal materials: pangolin is ground into fine powder, crosses 80 mesh sieves, and is standby.Radix Codonopsis, the seed of cowherb mix to be pulverized, and crosses the 40-80 mesh sieve, standby.

2, chingma abutilon seed is standby through frying.

3, other components are the pharmacopeia medicine materical crude slice, and are standby after the removal of impurities.

Two, preparation method

The decocting medicine extracts: the weight by component takes by weighing the Radix Astragali, Ligusticum wallichii, Radix Angelicae Sinensis, LIULUTONG, the stem pith of the rice-paper plant, Radix Rhapontici seu Radix Echinopsis, balloonflower root seven flavor medicine thing, adds 10 times of water gagings and decocts 1h, for the second time with add 8 times of water gagings respectively for the third time and respectively fry in shallow oil 1h, filter, merge three times decocting liquid, be concentrated into 1/90 of original volume, standby.

Radix Codonopsis, the seed of cowherb pulverized are mixed with concentrate, add pangolin fine powder and chingma abutilon seed, stir, 50-70 ℃ of oven dry, by every bag of 60g packing, sterilization, promptly.

Embodiment 2: as described in embodiment 1, different is the particle that is pressed into after stirring about diameter 5mm, makes granule, oven dry.

Embodiment 3: as described in embodiment 2, different is that component is as follows, is weight portion:

Radix Codonopsis 14 Ligusticum wallichiis 7 Radix Angelicae Sinensis 7 seeds of cowherb 12 LIULUTONGs 9

The stem pith of the rice-paper plant 6 Radixs Astragali 16 Radix Rhapontici seu Radix Echinopsis 9 chingma abutilon seeds 14 balloonflower roots 6

Embodiment 4: as described in embodiment 2, different is that component is as follows, is weight portion:

Radix Codonopsis 14 Ligusticum wallichiis 7 Radix Angelicae Sinensis 7 seeds of cowherb 12 LIULUTONGs 9 stem piths of the rice-paper plant 6

The Radix Astragali 16 Radix Rhapontici seu Radix Echinopsis 9 chingma abutilon seeds 14 dandelions 15 balloonflower roots 6

Embodiment 5: as described in embodiment 2, different is that component is as follows, is weight portion:

Radix Codonopsis 14 Ligusticum wallichiis 7 Radix Angelicae Sinensis 7 seeds of cowherb 12 LIULUTONGs 9 stem piths of the rice-paper plant 6

The Radix Astragali 16 Radix Rhapontici seu Radix Echinopsis 9 chingma abutilon seeds 14 balloonflower roots 6 dandelions 15 stir-bake to yellowish beans 12

Claims (6)

1, promotes the preparation of fodder for increasing milk, it is characterized in that it is the preparation of being made by following weight parts proportioning herbal raw material

Radix Codonopsis 10～20 Ligusticum wallichiis 4～10 Radix Angelicae Sinensis 4～10

The seed of cowherb 6～18 LIULUTONGs 5～15 stem piths of the rice-paper plant 4～10

The Radix Astragali 15～25 Radix Rhapontici seu Radix Echinopsis 6～15 chingma abutilon seeds 10～20

Dandelion 0～20 balloonflower root 1～8 pangolin 0～15

Stir-bake to yellowish beans 0～15

2, the preparation of promotion fodder for increasing milk as claimed in claim 1, wherein preferred weight part proportioning of each raw material is

Radix Codonopsis 15 Ligusticum wallichiis 8 Radix Angelicae Sinensis 8

The seed of cowherb 10 LIULUTONGs 10 stem piths of the rice-paper plant 8

The Radix Astragali 20 Radix Rhapontici seu Radix Echinopsis 10 chingma abutilon seeds 15

Balloonflower root 5

The combination 10 that dandelion or pangolin or stir-bake to yellowish beans or this three component are any two kinds

3, the preparation of promotion fodder for increasing milk as claimed in claim 1 is characterized in that described preparation is granule or pulvis.

4, the preparation method of the preparation of the described promotion fodder for increasing milk of claim 1 respectively will is characterized in that

A, Radix Codonopsis, the seed of cowherb and stir-bake to yellowish beans mix the back by proportioning to be pulverized, and sieves;

B, pangolin are worn into fine powder, sieve;

The frying before mixing of c, chingma abutilon seed; Then will

D, all the other raw material decoctings 3 times filter, merge filtrate, concentrated respectively three times; At last

E, a and d are merged, add b and c again, be processed into preparation behind the stirring and evenly mixing.

5, the preparation method of the preparation of promotion fodder for increasing milk as claimed in claim 4 is characterized in that the described fineness of sieving is 40～80 orders.

6, the preparation method of the preparation of promotion fodder for increasing milk as claimed in claim 4 is characterized in that the 1st, 2,3 decocting adds 10,8,8 times of water gagings successively respectively, respectively fries in shallow oil 1 hour.