CN1565174A - Artificial breeding method for fresh water shark - Google Patents
Artificial breeding method for fresh water shark Download PDFInfo
- Publication number
- CN1565174A CN1565174A CNA031262198A CN03126219A CN1565174A CN 1565174 A CN1565174 A CN 1565174A CN A031262198 A CNA031262198 A CN A031262198A CN 03126219 A CN03126219 A CN 03126219A CN 1565174 A CN1565174 A CN 1565174A
- Authority
- CN
- China
- Prior art keywords
- water
- shark
- parent population
- fish
- artificial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an artificial breeding method for fresh water sharks which mainly comprises the steps of, (1) parent fish source selection, (2) parent fish breeding, (3) artificial induced spawning, (4) incubation, and (5) sprout cultivating.
Description
Technical field
The present invention relates to artificial propagation of a kind of fresh water shark and seedling-cultivating method.
Background technology
Fresh water shark formal name used at school is a Su Shi circle abdomen fish (Pang sing, sutchi).Belong to Gyrinocheilidae,
Kind, mainly be distributed in Southeast Asia one band, be the good cultured freshwater fish on ground such as Thailand, Malaysia, the kind body colour of China's introduction at present is white shark and blue shark.The smooth no squama in this fish surface, the bodily form is long and flat-sided, has second adipose fin, is tropic fishes, and comfort zone is 12-36 ℃, and the growth optimum water temperature is 22-32 ℃.Advantages such as feeding habits are assorted, growth is fast, lower oxygen concentration resistance owing to have, and disease is few, output is high, and adaptability is strong, thorn is few between flesh in addition, fine and tender taste, deliciousness, characteristics such as nutritive value height, culture 1 year individuality and can reach the 1-2 kilogram, bigger reached at 2-3 kilogram, thereby have the value of popularization breed widely.But promote this fry kind mainly by import, improved aquaculture cost greatly, and be subjected to certain limitation, up to the present, the domestic report that does not also have about this fish artificial propagation success.
Summary of the invention
The purpose of this invention is to provide artificial propagation of a kind of fresh water shark and seedling-cultivating method.
Technical scheme of the present invention is: artificial propagation of a kind of fresh water shark and seedling-cultivating method, mainly comprise the following steps,
(1) in July, 1999 is formed marketable fish then from 3cm seed 5000 tails that Malaysia introduces in the parent population source, part listing, and part is stayed and is done parent population, and by calendar year 2001 1O month, the average individuality of parent population reaches the 3.5-4 kilogram, and maximum individuality reaches 5.5 kilograms.
(2) it is 50 meters the parent fish rearing parent fish rearing
2Cement pit, 1.7 meters of the depth of waters keep water quality pure and fresh, and the dissolved oxygen of water is higher, should remain on 4-6mgO
2/ L; PH6.8-7.4; Water temperature remains on 12-36 ℃, and forage feed guarantees fresh, never degenerates, and antenatal cultivation adds part Fructus Hordei Germinatus in addition again except that the special-purpose parent population feed of preparation, and fresh wild fishes etc. are to promote the gonad development of parent population.
(3) artificial induced spawning 3-11 month is carried out artificial induced spawning, and operation format adopts selects ripe parent population, is put in one 50 meters after hastening parturition
2Cement pit or net cage in, carry out stimulation by running water; The form of hastening parturition adopts the injection of two pins, and first pin is 1/3, the second a pin surplus of accumulated dose, grows the bad 3-4 of benefit again pin individually; The dry method fertilization is adopted in artificial insemination, and the ovum of extruding is spheroidal, and is light yellow, has viscosity, and the ovum footpath is 0.95-1mm approximately, and the parent population of egg laying amount 3.5-5.5 kilogram lays eggs between 650-1500g at every turn; Oxytocic drug adopts the mixture of hastening parturition.Injection back stimulation by running water is observed reproductive behavior, the record effect time, sticks at after laying eggs and carries out the flowing water hatching on net sheet and the brown sheet.
(4) hatching adopts the flowing water pond to hatch, water absorbent cotton filters during hatching, under water temperature 27-32 ℃ situation, approximately need 18-22 hour rupture of membranes, it is moving that the young behind the rupture of membranes is up and down string, very active, and just the rupture of membranes prelarva is transparence, individuality is very little, the about 1.7-2mm of length, juvenile fish is through the flat gradually trip of beginning in 48-56 hour, treats to begin to throw something and feed after the flat trip of most of fish body yolk and milk powder.
(5) the seed rearing fry hatches and promptly began flat trip in back second day, just can absorb to the belly yolk of the 3rd day prelarva and to finish, the prelarva initial feeding, this moment the agreeable to the taste feed of should in time throwing something and feeding because fish body individuality is too little, feeding method: opening material is yolk and milk material for the first time, begin to add fairy shrimp subsequently gradually, throw something and feed every day six times, long through 18-20 days take pains to foster to 2-3cm when seed, can change pond or sale.
Also comprise the following steps:
(6) processing of accelerating the ripening
The parent population processing of once accelerating the ripening January before hastening parturition, concrete grammar is: after the peanut oil heating, add No. 2 (LKH-A2) 2.5 microgram/kilograms of ovulation induction again, grind and make emulsion, intramuscular injection after the emulsification, the position is in the first dorsal fin downside intramuscular injection, and every endnote is penetrated the 2-3 milliliter.
The water quality of crawl allotment requirement is in the described step (2): well water 2/3, pure water 1/3.
Special-purpose parent population feed in the described step (2) is shark A-I number, and the primary raw material in its 50000 gram is: fish meal 15000 grams, dregs of beans 23000 grams, wheat bran 7000 grams, rice bran 3500 grams, calcium monohydrogen phosphate 1000 grams, salt 500 grams, vitamin E.
The mixture of hastening parturition in the described step (3) is: urge shark-A number, it is made up of hypophysis PG, human chorionic gonadtropin HCG, No. 2 LRH--A2 of ovulation induction, its proportioning is that every kilogram of fry needs: PG 2-5, and LRH--A2 6-10 microgram, HCG 400-600 international unit.
Optimum water temperature in the described step (2) remains on 22-32 ℃
Result of the test
Through test more than a year, reached our test objective substantially.No matter be parent fish rearing, artificial induced spawning hatching, seed rearing has all reached yielding ability level preferably, the parent fish rearing maturing rate reaches 85%, spawning rate reaches more than 96%, incubation rate reaches 60-80%, from having verified the reliability and stability of this technical matters, summed up the artificial propagation techniques data of the complete system of a cover, artificial propagation and seedling growing process obtain breaking through at home to make the fresh water shark.Hasten parturition altogether 52 batches of parent populations, accumulative total is hatched spray more than 1980.5 ten thousand tails.Be generalized to ground such as Hebei, Henan, Beijing, Tianjin after the cultivation.
Embodiment
For solving the artificial propagation problem, we are on the basis of culturing, begin the exploratory artificial propagation test of fresh water shark 2001 October, through priority repetition test, demonstration more than a year, obtained success, hasten parturition 52 batches altogether, accumulative total is hatched spray 1980.5 ten thousand tails, cultivates 2-3cm seed 29.8 ten thousand tails.Now the test situation with parent fish rearing, artificial induced spawning, seed rearing is reported as follows:
One, materials and methods
1, parent population source: then form marketable fish from external introduction 3cm seed 5000 tails in July, 1999, part listing, and part is stayed and is done parent population, and by October calendar year 2001, the average individuality of parent population reaches the 3.5-4 kilogram, and maximum individuality reaches 5.5 kilograms.
2, parent fish rearing and management
It is 50 meters parent fish rearing
2Cement pit, 1.7 meters of the depth of waters, owing to be power plant's hot water, water quality is better, and hardness of water is 8-9, and water exchange capacity is very big, and dissolved oxygen is also higher, can remain on 4-6mgO often
2/ L; PH7.2-7.4; Water temperature remains on 27-34 ℃.Forage feed guarantees fresh, never degenerates, and antenatal cultivation adds part Fructus Hordei Germinatus in addition again except that the special feed of preparation, and fresh wild fishes etc. are to promote the gonad development of parent population.
3, female, the male discriminating
Parent population is in mating season, and ripe parent population external form, feature are apparent in view, and the raun belly expands, and a little less than the pine, the ovary profile is obvious, gonopore expansion, ruddy, round pure, and milter gently squeezes gonopore has ripe milky seminal fluid to flow out.In addition milter was than the Zao maturation of raun 1 year, this batch fish milter just can be extruded seminal fluid in March, 2000, and dissect raun several times ovary all in the III beginning, strengthen cultivating and all get nowhere, when checking ovary once more the October of calendar year 2001, reached physiological maturity, the ovum grain is obviously free, promptly begins to prepare to hasten parturition.
4, artificial induced spawning
(1) hasten parturition the time: the time of hastening parturition is to finish from August 17 calendar year 2001 to November 4, begins on March 30th, 2002 to end by the end of November, and specifically the situation of hastening parturition sees attached list one.
(2) method of hastening parturition: because of belonging to the exploratory test of groping, do not have can reference and by the pattern of hastening parturition, so done groping of a large amount of experimental works and the form of hastening parturition at duration of test, a collection of sensitivity of all conscientiously observing every group of piscinity gland situation of change and sexual gland to medicine is whenever done in every group of dosage, every batch of oxytocic drug combination.Operation format adopts selects ripe parent population, is put in one 50 meters after hastening parturition
2Cement pit or net cage in, carry out stimulation by running water; The form of hastening parturition adopts the injection of two pins, and first pin is 1/3, the second a pin surplus of accumulated dose, grows the bad 3-4 of benefit again pin individually; The dry method fertilization is adopted in artificial insemination, and the ovum of extruding is spheroidal, and is light yellow, has viscosity, and the ovum footpath is 0.95-1mm approximately, and the parent population of egg laying amount 3.5-5.5 kilogram lays eggs between 650-1500g at every turn; Oxytocic drug adopts the mixture of hastening parturition.Injection back stimulation by running water is observed reproductive behavior, the record effect time, sticks at after laying eggs and carries out the flowing water hatching on net sheet and the brown sheet.
5, hatching
Hatching is adopted these 50 meters
2Hatch in the flowing water pond, and hatching water absorbent cotton filters, and approximately needs 18-22 hour rupture of membranes under water temperature 27-32 ℃ situation, and the young behind the rupture of membranes is that string is moving, very active up and down, and just the rupture of membranes prelarva is transparence, and individuality is very little, the about 1.7-2mm of length.Because the deficiency of hatching experience, early stage, incubation rate only had 10-40%; Along with the increase of lot number, operation skilled makes the later stage incubation rate be increased to 60-80%; Hatching depth of water 30-40cm is increased to about 60cm after hatching prelarva gradually, and juvenile fish is through the flat gradually trip of beginning in 48-56 hour, treats to begin to throw something and feed after the flat trip of most of fish body yolk and milk powder.
6, seed rearing
Fry hatches and promptly began flat trip in back second day, just can absorb to the belly yolk of the 3rd day prelarva and to finish, the prelarva initial feeding, this moment the agreeable to the taste feed of should in time throwing something and feeding, because fish body individuality is too little, feeding method: our the opening material first time is yolk and milk material, begin to add fairy shrimp subsequently gradually, throw something and feed every day six times, the concrete time was respectively 8 o'clock, 12 o'clock, 14 o'clock, 18 o'clock, 22 o'clock, 2 o'clock, however, prelarva just cuts one another's throat from the beginning in the 4th day of hatching, just take a favorable turn after to about the 1cm until the 10th day seed is long,, can change pond or sale through 18-20 days take pains to foster seed and can grow 2-3cm.
Two, result of the test
Through test more than a year, reached our test objective substantially.No matter be parent fish rearing, artificial induced spawning hatching, seed rearing has all reached yielding ability level preferably, the parent fish rearing maturing rate reaches 85%, spawning rate reaches more than 96%, incubation rate reaches 60-80%, from having verified the reliability and stability of this technical matters, summed up the artificial propagation techniques data of the complete system of a cover, artificial propagation and seedling growing process obtain breaking through at home to make the fresh water shark.Hasten parturition altogether 52 batches of parent populations, accumulative total is hatched spray more than 1980.5 ten thousand tails.Be generalized to ground such as Hebei, Henan, Beijing, Tianjin after the cultivation.
The parent fish rearing test report:
Parent fish rearing is the primary work of artificial propagation, to improving lethality in spawning rate, fertilization rate, incubation rate, the minimizing fry rearing process etc. direct influence is arranged all.So parent fish rearing work is the key point of fresh water shark artificial propagation, must accomplish sternly to grab tubule, strengthen raising, hold feeding quality and close, create a good living environment for parent population, make it The Gift of Being the Best You Can Be.This batch parent population is to cultivate from the seed oneself of the 2-3cm of Malaysia's introduction in July, 1999, and by August calendar year 2001, the average individuality of parent population has reached the 3.5-4 kilogram, maximum individual 5.5 kilograms.As far back as in March, 2000 milter ripe, raun is dissected ovary all in the III beginning, has possessed the parent population condition, and must strengthen cultivating, creating conditions promotes gonad development as early as possible, now that concrete parent fish rearing situation report is as follows:
One, parent fish pond condition
1, the parent fish rearing pond is 50 meters
2Cement pit, 1.7 meters of the depth of waters.
2, water condition: the water source is power plant's circulating hot water, and water exchange capacity is big, and water quality is better, PH7.2-7.4, hardness 5.5-9; Dissolved oxygen can remain on 4-6mgO often
2More than/the L, water temperature is controlled at 27-34 ℃.
Two, parent population stocking model and density
1, parent population stocking model and male and female are raised together with.
2, parent population breeding density is 50 square metres and throws in parent population 420 tails, i.e. every cubic metre of water body 8.4 tails, and the fish body weight is 33.6 kilograms every cubic metre, overall fish weight is 1680 kilograms.
Three, feed and throwing something and feeding
Feed is a material base of cultivating parent population, satisfies the required nutritional requirement of fish body, will play decisive role to its gonad development.The feed formula that the cultivation parent population is used is shark A-I number, its primary raw material is: fish meal, dregs of beans, wheat bran, salt, rice bran, calcium monohydrogen phosphate and additive etc., contain crude protein 34.8%, animal protein accounts for 30-40%, add part Fructus Hordei Germinatus, fresh wild fishes etc. again at product the first two months in addition, parent population is carried out reinforced cultivating.The throwing amount of raising is the 3-4% of fish body weight, throws something and feeds every day three times, is respectively at 8 o'clock in the morning, manages with delicacy by above-mentioned at 12 o'clock at noon, at 18 o'clock in afternoon, maintains strict control over the feeding quality pass, makes the parent population gonad development good, and the gonad maturity rate reaches more than 90%, has reached intended purposes.
Four, the management of parent fish pond and diseases prevention
1, the management of parent fish pond must accomplish to patrol round the clock the pool, and the one, control water temperature well, can not make water temperature too high or too low, the 2nd, can not allow parent fish pond anoxic occur, in case run into special circumstances generation anoxic, start the oxygen pump and inflate at once.
2, carry out diseases prevention: parent fish pond is regularly carried out diseases prevention work.We adhere to that all the time every first quarter moon carries out disinfection once with chlorine dioxide 0.5-1ppm; Carry out full pool spilling head once with enemy worm for fishing 0.5-1ppm in every month, and prevented and treated the generation of parasitosis.
Five, the discriminating of parent population maturity
The fine discriminating on the profile of ripe parent population, the milter bodily form is less, and belly has no change, and the raun bodily form is bigger, and belly changes greatly, and belly expands and justifies.From the also fine discriminating of reproductive organs: the similar mamillary of milter sexual organ, plumpness and projection expand and stiff; The similar circle of raun sexual organ, little red expansion usually, the ovary profile is obvious, and belly has upwards lifted jumping phenomena.Selecting to do when hastening parturition parent population, general raun belly expands, the ruddy open person of gonopore, and spawning rate can reach 100%.
Six, the processing of accelerating the ripening
The parent population processing of once accelerating the ripening January before hastening parturition, concrete grammar is: after the peanut oil heating, add No. 2 (LKH-A2) 2.5 microgram/kilograms of ovulation induction again, grind and make emulsion, intramuscular injection after the emulsification, the position is in the first dorsal fin downside intramuscular injection, and every endnote is penetrated the 2-3 milliliter.
High density intensification hatching and embryonic development and seedling growth test report:
Though the artificial propagation of fresh water shark is achieved success, has obtained a large amount of fertilized eggs.But fertilized egg need just might be finished embryonic development under certain environmental condition, hatch normal fry.Over 2 years, we have carried out a large amount of explorations and experimental study, seek to solve shark artificial propagation hatching optimised process and technology, and have completely solved this difficult problem, thereby fresh water shark versatility is finished.
Over 2 years, adopt high density intensification hatching technique, hatched prelarva 1980.5 ten thousand tails, wherein white shark seedling 1,450 ten thousand tails, blue shark 530.5 ten thousand tails, incubation rate has reached 60-80%, increases to educate 2-3cm shark seedling 29.8 ten thousand tails.
This technology mainly comprises: four parts such as research, hatching technique, embryonic development and seed rearing of the modulation of hatching water, high density intensification hatching facility.
(1) modulation of hatching water:
The hatching of fresh water shark is relatively stricter to water quality requirement, especially to hardness of water, acid-base value, pure and fresh degree.Make lots of expriments contrasting work during this period, because of the test site is the electric power plant circulating water water source, filter through strictness, hatching could be successful, do not become equally with the too high hatching of pure well water, hardness, acid-base value.In such cases, we have expected going to regulate water quality with pure water and well water, a collection of fish-egg is divided into the combination of several ratios and gropes gradually in fish jar, make it to find out a not only economy but also can hatch the water quality combination of prelarva, by the repetition test demonstration, water quality is combined as well water 2/3, pure water 1/3, a difficult problem has obtained solution, for producing accomplished the laying the foundation of shark seedling in batches.
(2) high density intensification hatching facility and hatching technique
1, incubation equipment
Cement pit, 49 meters of areas (7 meters * 7 meters)
2, add water 30-40cm, about 15 meters of water body
3
2, charger: utilize air pressure pump (60 liters/minute of air mass flows, pressure 0.15 kg/cm).
3, heating equipment: electricity adds water, and with 2 of electrical bars (each is 2500W), water temperature is controlled at 27-30 ℃, with canopy cloth cement pit is covered to keep room temperature.
4, purification of water quality: divide three layer filtration with space wadding.
(3) hatching technique and technology
1, adopts high density hydrostatic aeration, 49 meters
2Cement pit adds 15 meters in water
3, with 12 on gas head.
2, fertilized egg is a viscid egg, treats abundant after fertilization, evenly splashes on homemade rectangle reinforced frame screen cloth, evenly puts.
3, put ovum before, hatching water abundant aeration is put behind the ovum and to be checked fertilization rate in 5-6 hour, whole hatching process dissolved oxygen guarantees at 4-6mgO
2More than/the L.PH value 6.8-7.4.Hardness of water is 8-9.
4, put ovum density, each plate is put ten thousand of ovum 5-10 (specification of plate is 30-150cm).
5, hatching is water temperature: 26-32 ℃, and is the most suitable with 27-30 ℃.
6, fertilization rate height, the measured ovum of matter need not change water in hatching process, but fertilization rate is low, the relatively poor ovum of archiblast is many because of many, the middle ruptures of membranes of bad ovum at incubation process, easily cause water quality deterioration, so will in time replenish new water, in case influence hatching effect, must note observing because of the water quality ammonia nitrogen is high.
Four, embryonic development and seed rearing
(1) embryonic development
The fresh water shark bodily form is bigger, but the ovum grain is less, and ovum is 1-1.2mm directly, and 8-12 little oily ball arranged in the ovum, and transparent, water temperature was finished at 27-30 ℃ in embryonic development 18-22 hour.(because of being subjected to the restriction of condition and equipment, embryonic development aging schedule and the embryonic development collection of illustrative plates of shark fail to finish, and remedy when having ready conditions after remaining again).
(2) seed rearing
The prelarva total length that has just hatched is 1.7-2mm, begins flat trip through 48-56 hour prelarva major part, at this agreeable to the taste bait of will in time throwing something and feeding.First day opening throw something and feed yolk and milk powder, since the fairy shrimp or the wheel animalcule of the artificial incubation of throwing something and feeding in second day, throw something and feed 5-6 every day, impels the fish body to grow fast with the bait of abundance.Begin to increase gradually bath from bait throwing in, what we adopted is to add water round the clock when heating to 27-30 ℃ with pure well water, and it is fresh to remain Chi Shui, thereby reaches the purpose that as far as possible reduces the disease generation between seedling stage training.
According to observations, prelarva is from hatching the phenomenon that began to occur cutting one another's throat in the 4th day, and quite serious, and the bait abundance is also lost control of, and one just obviously reduces to 1cm the time.But when fish body 0.6-0.8cm, just 7-8 days of prelarva is dead peaks.
Through 10-20 days take pains to foster, the fish body can long 2-3cm, will reach the time-division pond at this and support temporarily, and enure can go on the market and sell or change over to adult fish culture.
The shark seedling of 2-3cm can the material feeding foam, but requires albumen than higher feed (crude protein requires to reach more than 32).As carp small fish Liao Huo Channel-catfish fish small fish material, soft-shelled turtle adult fish material etc.During this period, water temperature growth rate between 27-32 ℃ is very fast, takes pains to foster through a friend, and fish body total length can reach more than the 10cm.
Promote by seeds in 2002, survival rate generally reflects very high, and major part is all more than 60-85%.Be the seed that breeds at home after all, weather and geographical conditions are suitable, have improved several times to tens times with the weed survival rate of coming in abroad.This technical research has all reached the intended purposes of our test.
The engineering test of hastening parturition report:
Adopt the external source pharmacological method, make shark in this subenvironment of power plant's hot-tub, overcome all unfavorable factors, create and alternative fully natural environment habit, reach the sexual maturity of male and female fish ovaries spermary.But the obstacle that still has the mechanism of ovulation in artificial propagation has very big-difference owing to domestic with Southeast Asia one band border condition simultaneously, therefore still needs to study effective artificial induced spawning technology, just might obtain hasten parturition success and a large amount of fertilized eggs.
Various schemes tests were carried out in this research in years 2 years from calendar year 2001-2002, filtered out various physiology and proved repeated test, obtained stablizing effect, and spawning rate reaches more than 96%, has reached yielding ability level preferably.
(1) maturity of parent population is differentiated
Fresh water shark 3-4 can reach sexual maturity age naturally, and its milter is a maturation ahead of time.Its mode of appearance feature when the raun experimental group reaches maturation: belly expands softness, high resilience, and the ovary profile is obvious, and gonopore is open rubescent.
Ripe egg mother cell is ecru or apricot, and the ovum grain disperses in water, but belongs to viscid egg.
(2) artificial induced spawning technology
Before set forth, ripe fresh water shark does not have the particular environment can natural spawning under field conditions (factors), we adopt the pharmacological method of external source to reach the purpose of laying eggs in artificial induced spawning.
1, oxytocic drug test:
2001-2002 hastens parturition 52 groups altogether, it is made up of hypophysis PG, human chorionic gonadtropin HCG, No. 2 LRH--A2 of ovulation induction " to urge shark-A " with pure pharmacology oxytocic drug, its proportioning is that every kilogram of fry needs: PG 2-5, and LRH--A2 6-10 microgram, HCG 400-600 international unit.Through the testing and verification of hastening parturition repeatedly, therefrom filter out one group of the best scheme of hastening parturition, make it spawning rate and reach more than 96%, specifically the situation of hastening parturition sees Table one.
2, injecting method:
(1), lumbar injection
(2), female, male ratio: 1: 1
(3), two pins are injected first pin, 1/3 dosage, second pin, 2/3 surplus
(4), entry needle distance: 12 hours
(5), the 8-10 hour effect time
See table two for details.
(3) the maturation of ovum time
In the time of water temperature 27-34 ℃, egg mother cell is discharged from the folliculus film, keeps fertility about 1.2-2 hour in ovarian cavity.
(4) artificial insemination
Dry method is adopted in artificial insemination, and the effect time one checks once that to paying special attention to carry out microscopy after ovum can be extruded as early as possible, reaching fertilization time must firmly grasp per half an hour, and not so missing fertilization time will influence fertilization rate.Behind the maturation of ovum, with the metal washbowl, squeeze ovum earlier, clamp-on seminal fluid then, finish the time of fertilization and stir gently with feather and splash on brown sheet screen cloth, can change the hatching stage over to.
Ovum footpath, fertilized egg suction back is at 1-1.2mm, and concentrating in the ovum has 8-12 little oily ball.As the complete bright fertilization time that promptly just lost of ovum central authorities, all white ovum after 6 hours.
Fresh water shark artificial induced spawning test situation table
Table one:
Annotate: egg laying amount refers to average every tail g number of laying eggs.November 2, two groups of fishes were the parent population the used fully-developed again then of hastening parturition April.
Artificial induced spawning engineering test table
Table two:
Claims (6)
1, artificial propagation of a kind of fresh water shark and seedling-cultivating method is characterized in that: mainly comprise the following steps,
(1) in July, 1999 is formed marketable fish then from 3cm seed 5000 tails that Malaysia introduces in the parent population source, part listing, and part is stayed and is done parent population, and by October calendar year 2001, the average individuality of parent population reaches the 3.5-4 kilogram, and maximum individuality reaches 5.5 kilograms.
(2) it is 50 meters the parent fish rearing parent fish rearing
2Cement pit, 1.7 meters of the depth of waters keep water quality pure and fresh, and the dissolved oxygen of water is higher, should remain on 4-6mgO
2/ L; PH6.8-7.4; Water temperature remains on 12-36 ℃, and forage feed guarantees fresh, never degenerates, and antenatal cultivation adds part Fructus Hordei Germinatus in addition again except that the special-purpose parent population feed of preparation, and fresh wild fishes etc. are to promote the gonad development of parent population.
(3) artificial induced spawning 3-11 month is carried out artificial induced spawning, and operation format adopts selects ripe parent population, is put in one 50 meters after hastening parturition
2Cement pit or net cage in, carry out stimulation by running water; The form of hastening parturition adopts the injection of two pins, and first pin is 1/3, the second a pin surplus of accumulated dose, grows the bad 3-4 of benefit again pin individually; The dry method fertilization is adopted in artificial insemination, and the ovum of extruding is spheroidal, and is light yellow, has viscosity, and the ovum footpath is 0.95-1mm approximately, and the parent population of egg laying amount 3.5-5.5 kilogram lays eggs between 650-1500g at every turn; Oxytocic drug adopts the mixture of hastening parturition.Injection back stimulation by running water is observed reproductive behavior, the record effect time, sticks at after laying eggs and carries out the flowing water hatching on net sheet and the brown sheet.
(4) hatching adopts the flowing water pond to hatch, water absorbent cotton filters during hatching, under water temperature 27-32 ℃ situation, approximately need 18-22 hour rupture of membranes, it is moving that the young behind the rupture of membranes is up and down string, very active, and just the rupture of membranes prelarva is transparence, individuality is very little, the about 1.7-2mm of length, juvenile fish is through the flat gradually trip of beginning in 48-56 hour, treats to begin to throw something and feed after the flat trip of most of fish body yolk and milk powder.
(5) the seed rearing fry hatches and promptly began flat trip in back second day, just can absorb to the belly yolk of the 3rd day prelarva and to finish, the prelarva initial feeding, this moment the agreeable to the taste feed of should in time throwing something and feeding because fish body individuality is too little, feeding method: opening material is yolk and milk material for the first time, begin to add fairy shrimp subsequently gradually, throw something and feed every day six times, long through 18-20 days take pains to foster to 2-3cm when seed, can change pond or sale.
2, fresh water shark according to claim 1 artificial propagation and seedling-cultivating method is characterized in that: also comprise the following steps:
(6) processing of accelerating the ripening
The parent population processing of once accelerating the ripening January before hastening parturition, concrete grammar is: after the peanut oil heating, add No. 2 (LKH-A2) 2.5 microgram/kilograms of ovulation induction again, grind and make emulsion, intramuscular injection after the emulsification, the position is in the first dorsal fin downside intramuscular injection, and every endnote is penetrated the 2-3 milliliter.
3, fresh water shark according to claim 1 artificial propagation and seedling-cultivating method is characterized in that: the water quality of crawl allotment requirement is in the described step (2): well water 2/3, pure water 1/3.
4, fresh water shark according to claim 1 artificial propagation and seedling-cultivating method, it is characterized in that: the special-purpose parent population feed in the described step (2) is shark A-I number, primary raw material in its 50000 gram is: fish meal 15000 grams, dregs of beans 23000 grams, wheat bran 7000 grams, rice bran 3500 grams, calcium monohydrogen phosphate 1000 grams, salt 500 grams, vitamin E.
5, fresh water shark according to claim 1 artificial propagation and seedling-cultivating method, it is characterized in that: the mixture of hastening parturition in the described step (3) is: urge shark-A number, it is made up of hypophysis PG, human chorionic gonadtropin HCG, No. 2 LRH--A2 of ovulation induction, its proportioning is that every kilogram of fry needs: PG 2-5, LRH--A2 6-10 microgram, HCG 400-600 international unit.
6, fresh water shark according to claim 1 artificial propagation and seedling-cultivating method is characterized in that: the optimum water temperature in the described step (2) remains on 22-32 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031262198A CN1258972C (en) | 2003-06-23 | 2003-06-23 | Artificial breeding method for fresh water shark |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031262198A CN1258972C (en) | 2003-06-23 | 2003-06-23 | Artificial breeding method for fresh water shark |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1565174A true CN1565174A (en) | 2005-01-19 |
| CN1258972C CN1258972C (en) | 2006-06-14 |
Family
ID=34469058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031262198A Expired - Fee Related CN1258972C (en) | 2003-06-23 | 2003-06-23 | Artificial breeding method for fresh water shark |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1258972C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255606A (en) * | 2014-09-25 | 2015-01-07 | 苏州市相城区新时代特种水产养殖场 | Artificial breeding method of pangasius sutchi |
| CN106360149A (en) * | 2016-11-01 | 2017-02-01 | 耿明生 | Pangasius sutchi feed and preparation method thereof |
| CN108633788A (en) * | 2018-03-28 | 2018-10-12 | 南宁学院 | A kind of Pangasius sutchi low salinity stocking method |
-
2003
- 2003-06-23 CN CNB031262198A patent/CN1258972C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255606A (en) * | 2014-09-25 | 2015-01-07 | 苏州市相城区新时代特种水产养殖场 | Artificial breeding method of pangasius sutchi |
| CN106360149A (en) * | 2016-11-01 | 2017-02-01 | 耿明生 | Pangasius sutchi feed and preparation method thereof |
| CN108633788A (en) * | 2018-03-28 | 2018-10-12 | 南宁学院 | A kind of Pangasius sutchi low salinity stocking method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1258972C (en) | 2006-06-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100506033C (en) | Bionics artificial reproduction method of large salamander | |
| CN101502243B (en) | Large-scale cultivation method for summer fingerlings of Pelteobagrus fulvidraco | |
| CN101919359B (en) | Method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates | |
| CN104067959B (en) | A method of improving loach large size seedling seed cultivating survival rate | |
| CN105393985A (en) | Frog culturing method | |
| CN103875572B (en) | It is a kind of that soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method | |
| CN106900608A (en) | A kind of breeding method of big squama Barb fishes | |
| CN101720688A (en) | Snakehead breeding method | |
| CN102077806A (en) | Method for artificial propagation of giant salamanders and cultivation of baby giant salamanders | |
| CN105052782B (en) | The artificial fecundation method of Xichang Triplophysa | |
| CN102440204B (en) | Mixed breeding method for spotted maigre and large yellow croaker | |
| CN103891644A (en) | Preparation method for aquaculture carp breeding agents and breeding method | |
| CN106489801A (en) | A kind of half pierces thick lip fish artificial seedling rearing's method | |
| CN104642212A (en) | Artificial breeding method for onychostoma simus | |
| CN103493757B (en) | Method for breeding and cultivating four generations of fish and shrimp seedlings in pond in one year | |
| CN102919186B (en) | Artificial breeding method for sillago sihama | |
| CN109220903A (en) | A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method | |
| CN105557595A (en) | Cultivation method for brachymystax lenok fry | |
| CN1258972C (en) | Artificial breeding method for fresh water shark | |
| CN110839572A (en) | Artificial seedling culture method for babylonia areolata | |
| CN105724298A (en) | Method for factory puffer fish fry cultivation | |
| CN107079873B (en) | A kind of artificial propagation promoting method of Batrachuperus pinchonii | |
| CN103858794B (en) | The small-sized fertilized egg of fugu obscurus cultivates the method for fry | |
| CN109511589A (en) | A kind of Macrobrachium rosenbergii and Bellamya sp pond polyculture method | |
| CN110506678B (en) | Integrated feeding technology-based carassius auratus gibelio feeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060614 Termination date: 20140623 |
|
| EXPY | Termination of patent right or utility model |