CN1561690A - Ecological regulating method for seedling growing of porphyra haitanensis - Google Patents
Ecological regulating method for seedling growing of porphyra haitanensis Download PDFInfo
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- CN1561690A CN1561690A CN 200410017250 CN200410017250A CN1561690A CN 1561690 A CN1561690 A CN 1561690A CN 200410017250 CN200410017250 CN 200410017250 CN 200410017250 A CN200410017250 A CN 200410017250A CN 1561690 A CN1561690 A CN 1561690A
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Abstract
An ecological regulation method for breeding laver in earthen jar features that it is washed and its water is exchanged once per 40-50 days, the illuminant intensity and time and the concentration of nutritive salt are increased for increasing the number of microseaweeds, or the average density of copepod is artificially regulated by artificial inoculation or water exchange.
Description
Technical field
The present invention relates to the method that a kind of Porphyra haitanensis is grown seedlings, especially relate to the ecological control method that a kind of Porphyra haitanensis is grown seedlings.
Background technology
The laver approach of growing seedlings mainly contains following three kinds at present: the one, and the direct Cheng Miao of free conchocelis, the laver carpospore can not pierce shell, and can in culture fluid, directly form filamentous, the suspension culture free conchocelis generation conchospore that grows, (Chen Guoyi is about the research that the Porphyra haitanensis free conchocelis is cultivated and directly collected seedling, aquatic product journal to grow the laver seedling, 1980,4 (1): 19~29).By breeding porphyra yezoensis sporangium branch clone and to the implementation developmental regulation research also arranged.Impel the porphyra yezoensis filamentous maturation of suspension culture and diffuse conchospore with the method for ventilation, and then develop into normal seedling, this method is not used in actual production basically.The 2nd, laver cell engineering enzyme process is grown seedlings, and is to select the good thallus of trait expression as somatic source, need not consider whether thallus is ripe.The somatic cell that obtains through enzymolysis is used as grow seedlings (Dai Jixun etc., 1988, the enzyme process separation of thallus cell of laver and breed research thereof, biotechnology journal, 4 (2) 133~137).The period of growing seedlings only for the tradition grow seedlings 1/5, save a large amount of time, space, manpower and material resources have favorable economic benefit.Mode by refrigeration is preserved thallus, in conjunction with temperature control control light, can grow seedlings at any time.But, the quality of enzyme and limited amount, technical problems such as the stability of growing seedlings await further to test.The 3rd, utilize the early stage algal filament cell inoculation shell of free conchocelis, cultivate the shell conchocelis of laver, produce a large amount of conchospores by shell conchocelis again and produce seedling, improved seeds are imported the technical system of producing, obtained tangible progress (Ceng Chengkui, economical alga germplasm seedling biology, Shandong science tech publishing house, 1992.).This process also involves the cultivation of shell conchocelis, also is a kind of method comparatively commonly used at present.The cultivation of the shell conchocelis of traditional Porphyra haitanensis is carried out in seedling rearing room, period be April from every year to September, whole seedling raise period reaches 5 months.Owing to exist one or more benthic algaes or planktonic algae in the water body of cultivating, growth is rapid, and the density height by competing nutritive salt with conchocelis of porphyra, has influenced the absorption of shell conchocelis to nutritive salt; Cover shell conchocelis, influenced the surface that light reaches shell conchocelis, weakened filamentous photosynthesis, cause the shell conchocelis can not to be covered with shell, poor growth.The measure of generally taking is: every two weeks will carry out scrubbing of a shell conchocelis, changes water, turns around, to alleviate the influence of benthic algae or planktonic algae.Like this, waste time and energy, consume great amount of manpower and material resources, it is too small to change water, scrub untimely, cause benthic algae or planktonic algae on shell conchocelis surface a large amount of the appearance, influence the normal growth of filamentous; It is excessive to change water, scrubs too quicklyly, causes cost to increase, and reveals emptyly for a long time, influences the survival rate of shell conchocelis.Adopt the method for the growth that suppresses little algae, such condition also can be unfavorable for the growth of filamentous.
Summary of the invention
Technical problem to be solved by this invention is the ecological control method that provides a kind of Porphyra haitanensis to grow seedlings at above-mentioned prior art present situation, can improve the survival rate of shell conchocelis effectively, and it is time saving and energy saving, with low cost, can lower simultaneously eutrophication pressure, have favorable economic benefit, society and ecological benefits for environment.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: the ecological control method that a kind of Porphyra haitanensis is grown seedlings, in Porphyra haitanensis shell conchocelis incubation, increase or reduce the averag density of copepoda, the averag density that keeps more than one copepoda is 3000~30000/square metre water bodys.
In Porphyra haitanensis shell conchocelis incubation, the number of times of scrubbing and change water can be per 40~50 days once, each quantity of exchanged water can be 35%~65% of Total Water, and the number of times of scrubbing and change water is preferably per 45 days once, and each quantity of exchanged water is 50% of a Total Water.
The increase of the averag density of described copepoda also can be by increasing intensity of illumination and prolonging light application time or concentration by the salt that has additional nutrients increases dwell little algae or planktonic microalgae quantity of the end and realizes.
The increase of the averag density of described copepoda can realize by the mode of artificial inoculation.
The minimizing of the averag density of described copepoda realizes by changing water, also can be by reducing intensity of illumination and shortening light application time or reduce dwell little algae or planktonic microalgae quantity of the end by the concentration that reduces nutritive salt and realize.
Advantage: by in water, keeping the copepoda of proper density, reach zooplankton and benthic algae or planktonic algae dynamic equilibrium in the water body, on this basis, form ecology regulation and control that Porphyra haitanensis grows seedlings technical system, the shell conchocelis growth that guarantees laver is normal with zoon, can also lower the eutrophication pressure for environment; Reduce and to change the waterside number, increase the cycle of cultivating, can also save and scrub the manpower that changes water and the consumption of material resources and the energy and water.The present invention not only has certain economic benefits, also has good social benefit and ecological benefits.
With 500 square metres areas is example, and common grows seedlings, and changes water once in general 15 days, and each water is 400t, and the energy consumption that needs is 120 yuan, scrubs, and changes water, turn around once, requirement skillfully manually be 12 workers, with 30 yuan of calculating of every worker, need 360 yuan.Grow seedlings period whole, carry out 8 times, the seawater that needs is 3200t altogether, needs 960 yuan, and labour cost is 2880 yuan.Amount to 3840 yuan; And employing the present invention, total quantity of exchanged water is 600t, the energy consumption that needs is 180 yuan, scrubs and turns around 2 times, needs 720 yuan, is 1/4 of common seedling management cost.
Embodiment
By the following examples the present invention is described in further detail.
Embodiment one: 500 square metres of the areas that Porphyra haitanensis is grown seedlings, the degree of depth in pond are 0.8 meter, inoculation on April 10th, 2003 carpospore, change water 35% in per 40 days, and shell surface does not have mud, the 2/mm of averag density of benthic algae or planktonic algae
2, the averag density of copepoda is 3000/square metre water bodys, mainly contains very wise by force water flea in Calanus sinicus (Calanussinicus), Asia (Eucalanus subcrassus) and Pacific Ocean spindle water flea (Acartia pacifica).Grow seedlings season whole, change water 3 times, total quantity of exchanged water is 420t, and to 30~September 5 August, shell conchocelis is reached maturity, and produces conchospore, can satisfy the needs that yielding ability is grown seedlings.Grow seedlings 500 mu, the seed well-grown.
Embodiment two: 500 square metres of the areas that Porphyra haitanensis is grown seedlings, the degree of depth in pond are 0.8 meter, inoculation on April 10th, 2003 carpospore, change water 50% in per 45 days, and shell surface does not have mud, the 4/mm of averag density of benthic algae or planktonic algae
2, the averag density of copepoda is 6000/square metre water bodys, mainly contains very wise by force water flea in Calanus sinicus (Calanussinicus), Asia (Eucalanus subcrassus) and exquisite true thorn water flea (Euchaeta concinna).Grow seedlings season whole, change water 3 times, total quantity of exchanged water is 600t, and to September 3~12, shell conchocelis was reached maturity, and produced conchospore, can satisfy the needs that yielding ability is grown seedlings.Grow seedlings 500 mu, the seed well-grown.
Embodiment three: 500 square metres of the areas that Porphyra haitanensis is grown seedlings, the degree of depth in pond are 0.8 meter, inoculation on April 10th, 2003 carpospore, change water 65% in per 50 days, and shell surface does not have mud, the 6/mm of density of benthic algae or planktonic algae
2, the averag density of copepoda is 10000/square metre water bodys, mainly contains the very wise by force water flea in Asia (Eucalanussubcrassus), exquisite true thorn water flea (Euchaeta concinna) and Pacific Ocean spindle water flea (Acartia pacifica).Grow seedlings season whole, change water 2 times, total quantity of exchanged water is 520t, and to 28~September 3 August, shell conchocelis is reached maturity, and produces conchospore, can satisfy the needs that yielding ability is grown seedlings.Grow seedlings 500 mu, the seed well-grown.
Embodiment four: 350 square metres of the areas that Porphyra haitanensis is grown seedlings, the degree of depth in pond are 0.8 meter, inoculation on April 3rd, 2003 carpospore, change water 45% in per 40 days, and shell surface does not have mud, the 8/mm of density of benthic algae or planktonic algae
2The density of copepoda is 20000/square metre water bodys, mainly containing the exquisite true thorn water flea of Calanus sinicus (Calanus sinicus), the very wise by force water flea in Asia (Eucalanus subcrassus) (Euchaeta concinna) grows seedlings season whole, change water 3 times, total quantity of exchanged water is 378t, and to September 2~12, shell conchocelis was reached maturity, produce conchospore, can satisfy the needs that yielding ability is grown seedlings.Grow seedlings 350 mu, the seed well-grown.
Embodiment five: 350 square metres of the areas that Porphyra haitanensis is grown seedlings, the degree of depth in pond are 0.8 meter, inoculation on April 3rd, 2003 carpospore, change water 55% in per 45 days, and shell surface does not have mud, the 10/mm of density of benthic algae or planktonic algae
2The density of copepoda is 30000/square metre water bodys, mainly containing Calanus sinicus (Calanus sinicus), the very wise by force water flea in Asia (Eucalanus subcrassus), exquisite true thorn water flea (Euchaeta concinna) and Pacific Ocean spindle water flea (Acartia pacifica) grows seedlings season whole, change water 3 times, total quantity of exchanged water is 462t, and to September 2~12, shell conchocelis was reached maturity, produce conchospore, can satisfy the needs that yielding ability is grown seedlings.Grow seedlings 350 mu, the seed well-grown.
In the above embodiments, the increase of the averag density of copepoda can also increase dwell little algae or planktonic microalgae quantity of the end by the concentration that increases intensity of illumination and prolong the light application time or the salt that has additional nutrients and realize, also can realize by the mode of artificial inoculation.The minimizing of the averag density of copepoda then can realize by changing water, also can be by reducing intensity of illumination and shortening light application time or reduce dwell little algae or planktonic microalgae quantity of the end by the concentration that reduces nutritive salt and realize.
Claims (5)
1, a kind of Porphyra haitanensis ecological control method of growing seedlings is characterized in that in Porphyra haitanensis shell conchocelis incubation, increases or reduce the averag density of copepoda, and the averag density that keeps more than one copepoda is 3000~30000/square metre water bodys.
2, a kind of Porphyra haitanensis according to claim 1 ecological control method of growing seedlings is characterized in that in Porphyra haitanensis shell conchocelis incubation, the number of times of scrubbing and change water be per 40~50 days once, each quantity of exchanged water is 35%~65% of a Total Water.
3, a kind of Porphyra haitanensis according to claim 2 ecological control method of growing seedlings is characterized in that in Porphyra haitanensis shell conchocelis incubation, the number of times of scrubbing and change water be per 45 days once, each quantity of exchanged water is 50% of a Total Water.
4, a kind of Porphyra haitanensis according to claim 1 ecological control method of growing seedlings, the increase that it is characterized in that the averag density of described copepoda are that the mode by artificial inoculation realizes.
5, a kind of Porphyra haitanensis according to claim 1 ecological control method of growing seedlings is characterized in that the minimizing of the averag density of described copepoda realizes by changing water.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102845296A (en) * | 2012-03-26 | 2013-01-02 | 宁波大学 | Releasing method for porphyra haitanensis seedlings |
CN103999763A (en) * | 2014-04-18 | 2014-08-27 | 中国科学院海洋研究所 | Method for clearing wild algae contamination in live history micro-phase of large economic algae |
CN104067928A (en) * | 2014-06-25 | 2014-10-01 | 无锡市崇安区科技创业服务中心 | Culturing method of nori seedlings |
CN105379613A (en) * | 2015-11-28 | 2016-03-09 | 王谷安 | Laver culturing rope capable of preventing laver culturing raft frame from being attached with hybrid algae |
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2004
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102845296A (en) * | 2012-03-26 | 2013-01-02 | 宁波大学 | Releasing method for porphyra haitanensis seedlings |
CN102845296B (en) * | 2012-03-26 | 2014-11-05 | 宁波大学 | Releasing method for porphyra haitanensis seedlings |
CN103999763A (en) * | 2014-04-18 | 2014-08-27 | 中国科学院海洋研究所 | Method for clearing wild algae contamination in live history micro-phase of large economic algae |
CN103999763B (en) * | 2014-04-18 | 2016-01-20 | 中国科学院海洋研究所 | A kind ofly remove the large-scale economical alga microcosmic history of life stage and to mix the method for algae pollution |
CN104067928A (en) * | 2014-06-25 | 2014-10-01 | 无锡市崇安区科技创业服务中心 | Culturing method of nori seedlings |
CN105379613A (en) * | 2015-11-28 | 2016-03-09 | 王谷安 | Laver culturing rope capable of preventing laver culturing raft frame from being attached with hybrid algae |
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