CN1561687A - 一种无菌培养沉水植物的方法 - Google Patents
一种无菌培养沉水植物的方法 Download PDFInfo
- Publication number
- CN1561687A CN1561687A CNA2004100128637A CN200410012863A CN1561687A CN 1561687 A CN1561687 A CN 1561687A CN A2004100128637 A CNA2004100128637 A CN A2004100128637A CN 200410012863 A CN200410012863 A CN 200410012863A CN 1561687 A CN1561687 A CN 1561687A
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- CN
- China
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- explant
- medium
- culture
- liquid nutrient
- nutrient medium
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- Granted
Links
- 238000000034 method Methods 0.000 title claims description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 229920001817 Agar Polymers 0.000 claims abstract description 7
- 239000008272 agar Substances 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims abstract description 4
- 238000012258 culturing Methods 0.000 claims abstract description 3
- 239000002609 medium Substances 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 20
- 239000007787 solid Substances 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000007943 implant Substances 0.000 claims description 3
- 238000011177 media preparation Methods 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 abstract 2
- 230000001112 coagulating effect Effects 0.000 abstract 1
- 238000012136 culture method Methods 0.000 abstract 1
- 239000002355 dual-layer Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 21
- 241000195493 Cryptophyta Species 0.000 description 6
- 240000004590 Potamogeton malaianus Species 0.000 description 5
- 241001123263 Zostera Species 0.000 description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 229940064880 inositol 100 mg Drugs 0.000 description 2
- 235000001968 nicotinic acid Nutrition 0.000 description 2
- 229960003512 nicotinic acid Drugs 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000001863 plant nutrition Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 229960000344 thiamine hydrochloride Drugs 0.000 description 2
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 2
- 239000011747 thiamine hydrochloride Substances 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000009602 toxicology test Methods 0.000 description 1
Classifications
-
- Y02P60/216—
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Hydroponics (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2004100128637A CN1258968C (zh) | 2004-03-20 | 2004-03-20 | 一种无菌培养沉水植物的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2004100128637A CN1258968C (zh) | 2004-03-20 | 2004-03-20 | 一种无菌培养沉水植物的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1561687A true CN1561687A (zh) | 2005-01-12 |
CN1258968C CN1258968C (zh) | 2006-06-14 |
Family
ID=34478019
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004100128637A Expired - Lifetime CN1258968C (zh) | 2004-03-20 | 2004-03-20 | 一种无菌培养沉水植物的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1258968C (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102450150A (zh) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | 篦齿眼子菜繁殖技术 |
CN103053422A (zh) * | 2013-01-09 | 2013-04-24 | 成都华都农业发展有限责任公司 | 繁殖穿叶眼子菜的方法 |
CN104719110A (zh) * | 2015-02-15 | 2015-06-24 | 浙江大学 | 一种基于作物-藻共生作用的生态水培方法 |
CN109699443A (zh) * | 2019-02-25 | 2019-05-03 | 重庆大学 | 一种沉水植物种植基质和使用该基质种植沉水植物的方法 |
CN115553184A (zh) * | 2022-11-03 | 2023-01-03 | 中国科学院水生生物研究所 | 一种促进沉水植物苦草种子萌发及幼苗生长的方法 |
CN115581175A (zh) * | 2022-11-03 | 2023-01-10 | 中国科学院水生生物研究所 | 一种促进沉水植物苦草种子萌发和幼苗生长的方法 |
-
2004
- 2004-03-20 CN CNB2004100128637A patent/CN1258968C/zh not_active Expired - Lifetime
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102450150A (zh) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | 篦齿眼子菜繁殖技术 |
CN102450150B (zh) * | 2010-11-01 | 2013-05-22 | 南京中科水治理股份有限公司 | 篦齿眼子菜繁殖技术 |
CN103053422A (zh) * | 2013-01-09 | 2013-04-24 | 成都华都农业发展有限责任公司 | 繁殖穿叶眼子菜的方法 |
CN103053422B (zh) * | 2013-01-09 | 2014-03-26 | 成都华都农业发展有限责任公司 | 繁殖穿叶眼子菜的方法 |
CN104719110A (zh) * | 2015-02-15 | 2015-06-24 | 浙江大学 | 一种基于作物-藻共生作用的生态水培方法 |
CN109699443A (zh) * | 2019-02-25 | 2019-05-03 | 重庆大学 | 一种沉水植物种植基质和使用该基质种植沉水植物的方法 |
CN115553184A (zh) * | 2022-11-03 | 2023-01-03 | 中国科学院水生生物研究所 | 一种促进沉水植物苦草种子萌发及幼苗生长的方法 |
CN115581175A (zh) * | 2022-11-03 | 2023-01-10 | 中国科学院水生生物研究所 | 一种促进沉水植物苦草种子萌发和幼苗生长的方法 |
Also Published As
Publication number | Publication date |
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CN1258968C (zh) | 2006-06-14 |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Guangzhou Rongchuan Feeds Co.,Ltd. Assignor: Institute of Hydrobiology, Chinese Academy of Sciences Contract fulfillment period: 2007.2.1 to 2012.1.31 Contract record no.: 2008420000015 Denomination of invention: Method for aseptic cultivating submerged plant Granted publication date: 20060614 License type: Exclusive license Record date: 20080707 |
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Free format text: EXCLUSIVE LICENCE; TIME LIMIT OF IMPLEMENTING CONTACT: 2007.2.1 TO 2012.1.31 Name of requester: YUNG-CHUAN FEED CO., LTD., GUANGZHOU CITY Effective date: 20080707 |
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Assignee: Guangzhou Rongchuan Feeds Co.,Ltd. Assignor: Institute of Hydrobiology, Chinese Academy of Sciences Contract record no.: 2008420000015 Date of cancellation: 20150228 |
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EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20050112 Assignee: WUHAN ZHONGKE HYDRBIOLOGY ENVIRONMENT ENGINEERING CO.,LTD. Assignor: Institute of Hydrobiology, Chinese Academy of Sciences Contract record no.: 2015420000048 Denomination of invention: Method for aseptic cultivating submerged plant Granted publication date: 20060614 License type: Exclusive License Record date: 20150414 |
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LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
CX01 | Expiry of patent term | ||
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Granted publication date: 20060614 |