CN1546644A - Production method of Chinese caterpillar fungus powder and active Chinese caterpillar fungus polysaccharide freeze-drying powder for immunity, and product thereof - Google Patents
Production method of Chinese caterpillar fungus powder and active Chinese caterpillar fungus polysaccharide freeze-drying powder for immunity, and product thereof Download PDFInfo
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- CN1546644A CN1546644A CNA2003101189883A CN200310118988A CN1546644A CN 1546644 A CN1546644 A CN 1546644A CN A2003101189883 A CNA2003101189883 A CN A2003101189883A CN 200310118988 A CN200310118988 A CN 200310118988A CN 1546644 A CN1546644 A CN 1546644A
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Abstract
In the invention, the life history characteristic of Chinese caterpillar fungus is studied, and tissue cell culturing technology is employed to obtain phorozoon cell of Chinese caterpillar fungus from the natural Chinese caterpillar fungus fruiting body through isolation, carrying out purification and domestication, utilizing automatic control fermenting method, spray drying, cytoclasis to produce Chinese caterpillar fungus bacterium powder, wherein the active immunological Chinese caterpillar fungus polysaccharide freeze-drying powder can be prepared from Chinese caterpillar fungus bacterium powder as raw material the steps of complex enzyme extraction and adsorption.
Description
Content of the present invention belongs to biochemical article preparing technical field, relates to a kind of method and product that utilizes method that Cordyceps strain prepares Cordyceps powder and product and the above bacterium powder raw material of a kind of basis to extract Chinese caterpillar fungus immunocompetence polysaccharide.
Cordyceps sinensis (Cordyceps sinensis) is a kind of famous and precious edible and medicinal fungi that is distributed in provinces and regions high mountain such as China Sichuan, Tibet, Qinghai, Yunnan, Gansu and subalpine meadow area, belongs to Ascomycetes, Clavicipitaceae.Because compositions such as contained cordycepic acid and cordycepin have expansion tracheae, calmness, effect such as antibiotic and hypotensive in the Cordyceps sinensis, be used as medicine with exsiccant stroma and polypide on the traditional Chinese medicine, can be used for treatment and consider various disease conditions such as impairment and cough phlegm blood, asthma, pain in the back, pulmonary tuberculosis, chronic nephritis, diabetes, tumour, seminal emission impotence, spontaneous sweating, insomnia forgetfulness, Chinese caterpillar fungus itself also contains multiple human body essential amino acid and the necessary vitamins B of human body hematopoiesis in addition
12, thereby it has splendid medical treatment and nourishing function, and in recent years, society's being in great demand to it.In view of the residing growing environment of wild cordyceps is high and cold particular surroundings; its host bat moth needs just can finish epoch more than 3 years; cause the worm grass resources deficiency of natural propagation; produce for contradiction originally very outstanding; artificial in recent years in addition excessively digging; ecotope is seriously damaged, and some geographic Cordyceps sinensis have been on the verge of exhaustion, and setting up effective sfgd. also has been very urgent.Therefore; this area demands seeking out the anamorph cell paste and products thereof of the Cordyceps sinensis of novel Tong Guo suitability for industrialized production urgently; make it become the surrogate of natural cordyceps sporophore; to alleviate the imbalance between supply and demand of Chinese medicinal materials; preserve the ecological environment, also enter the world market simultaneously and produce road of high value added product wound for China's herbal medicine.
One of purpose of the present invention is at the prior art present situation, and a kind of Cordyceps fungus powder and production method thereof of alternative natural cordyceps is provided; It is that raw material extracts kind of the production method and the product of active immne Cordyceps polysaccharide lyophilized powder that another object of the present invention is to provide more than one to state the Cordyceps fungus powder product.
Be used to realize that the whole design idea of foregoing invention purpose is such: the feature of dividing teleomorph and anamorph according to the life history of Cordyceps sinensis, adopt modern histocyte culture technique, separate the anamorph cell that obtains Cordyceps sinensis from the sexual sporophore of natural cordyceps, after it is carried out purifying and instructionization, utilize the automatic operations such as fermentation method and spraying drying, cytoclasis of controlling to produce Cordyceps fungus powder; Then be that raw material is prepared active immne Cordyceps polysaccharide lyophilized powder by operations such as prozyme extraction method and absorption methods with the Cordyceps fungus powder.
Production method of the present invention and product comprise according to natural cordyceps sporophore pure strain and prepare the production method of Cordyceps fungus powder and product and be two kinds of the production method of feedstock production active immne Cordyceps polysaccharide lyophilized powder and products with the Cordyceps fungus powder.
The method of production Cordyceps fungus powder of the present invention is made of following production process:
(1), the acquisition of good pure bacterial strain bacterial classification: the fresh individuality that selects the natural cordyceps sporophore, from bacterial strain, filter out the bacterial strain of relatively stable and better performances with the primary dcreening operation of routine and multiple sieve method, then successively by good relatively peacilomyce hepiahi (the paecilomyces hepiali Chen ﹠amp of the isolating method acquisition of conventional microfloras such as separation, ultraviolet mutagenesis, rejuvenation and contrast; Dai) bacterial classification;
(2), use the fermentative Production winter worm summer herb thallus: comprise two kinds of solid fermentation method and solution fermentations, wherein:
A, solid fermentation working system---making the bacterial classification that obtains through operation () is that (per unit part material formula of this comprehensive PDA culture medium is: 20 weight unit of mealy potato for 6.8～7.2 comprehensive PDA culture medium at pH value, 20 weight unit of sucrose, 1.5 weight unit of potassium primary phosphate, 1 weight unit of sal epsom, vitamins B
10.02 individual weight unit, 1.5 weight unit of native protein, 30 weight unit of wheat bran, 10 weight unit of Semen Maydis powder.) go up inoculation, and make it to do solid fermentation cultivation 15～20 days with substratum, culture temperature is 16～25 ℃, humidity 70%; Thereafter thalline is carried out cryodrying, temperature obtains dry thalline below 60 ℃;
B, liquid fermenting working system---will insert pH value through the bacterial classification that operation () obtains is that (prescription of this comprehensive PDA culture medium liquid is: mealy potato 20g, sucrose 20g, potassium primary phosphate g, sal epsom 1g, vitamins B for 6.8～7.2 comprehensive PDA culture medium liquid
120mg, native protein 0.5g, wheat bran 30g, Semen Maydis powder 10g, water 1000ml.) inoculate in the jar fermenter, and make it in liquid medium, to do submerged fermentation and cultivated 5～7 days, culture temperature is 16～25 ℃; Thereafter the method by evaporation concentration and Plate Filtration is separated the thalline after liquid medium and the growth, and isolated thalline is carried out spraying drying, obtains dry thalline;
3., pulverize and produce ultrafine powder: will make the ultrafine powder of granularity for the Cordyceps fungus powder of-300 orders (particle diameter is 4～10 microns) through the dry thalline that operation (two) obtains with the cell pulverization machine, in the crushing process, envrionment temperature is no more than 60 ℃;
(4), end properties detects, packs, puts in storage.
Bacterium powder cell wall breaking rate＞95% of the product of Cordyceps fungus powder of the present invention for making through above-mentioned operation ()～(four).Because cell and micro mist are the bases that pharmaceuticals improve drug effect, in, the main pharmacodynamics composition of Western medicine all is distributed in the cell usually and in the intercellular substance, and in cell, behind cell wall breaking, effective constituent comes out in its cell, the cell wall breaking rate is high more, and the drug effect of product is just high more, and onset speed is also fast more.The determination and analysis result who bacterium powder finished product is done through units such as Shaanxi Province's examination and test of products institute, Shaanxi Province health and epidemic prevention station, the Shaanxi Province academy of traditional Chinese medicine, the attached medical colleges in Shanghai Communications University, Xi'an shows: the content of this product inner nucleotide class material, N.F,USP MANNITOL, active immne Cordyceps polysaccharide etc. all is greater than or equal to the content of natural cordyceps, it also contains each seed amino acid, trace element and organoselenium etc. in addition, its sanitary index meets national standard, and antitumous effect is obvious.Thisly not only reach the performance that can substitute natural cordyceps from chemical constitution, nutrition, pharmacological action by the Cordyceps fungus powder that manually makes, and the tissue morphology of its product also reached the peak demand of international pharmaceutical industry to Chinese medicine, but the product one-time-reach-place is directly integrated with the world market.
The finished product of the present invention are to utilize Cordyceps fungus powder to extract immunocompetence polysaccharide and other product.In recent years, a lot of in this area about fungi immunocompetence STUDY ON POLYSACHAROSE report, but product seldom, and its major cause is exactly to extract at present the yielding poorly of polysaccharide production technique (as hot water extraction method, organic solvent extraction method, conventional enzyme extraction method etc.), cost height, active low.The present invention is directed to the defective of prior art, adopt the extracting method of combined-enzyme method as Cordyceps polysaccharide, this method has overcome prior art and has destroyed the three-dimensional arrangement of bioactive molecules and reduce shortcoming such as biological activity ability in leaching process, improved extraction yield, obtain the high Cordyceps polysaccharide of immunocompetence, also reduced production cost simultaneously.Operational path by Cordyceps fungus powder production immunocompetence Cordyceps polysaccharide is: under being lower than 40 ℃ of conditions 1 part of proteolytic ferment, 1～2 part of amylolytic enzyme and 1 part of cellulolytic enzyme are added respectively in 10 parts of Cordyceps fungus powders, be under 75% the condition it to be stirred in water content, then be after 25～40 ℃ and pH value are to act on 45～90 minutes under 6.0～8.0 conditions in temperature, adopt conventional organic solvent precipitation method to extract polysaccharide component in the above-mentioned material; Obtaining content by the frozen drying method again is 75～85% polysaccharide raw product; Then utilize proteolytic ferment, amylolytic enzyme and cellulolytic enzyme to extract again with the raw product effect respectively again, and utilize absorption method that extract is again decoloured and handle the back and obtain content at the refinishing polyose product more than 95%, again with after the freeze-drying of refinishing polyose product, obtain active immne Cordyceps polysaccharide lyophilized powder at last.
Compare with traditional extraction polysaccharide method, utilize reaction conditions gentleness, the enzyme reaction character of combined-enzyme method extraction polysaccharide single-minded, under general condition, can not destroy or influence 1 of the three-dimensional arrangement of polysaccharide and macromolecular main chain structure, the poly-hydroxy group that anti-tumor activity is played an important role on the 3-glycosidic link skeleton, thus reach the impregnable purpose of protection active group physiological activity; In addition, the reaction times of prozyme extraction method weak point, enzyme source can utilize saccharifying enzyme production, and it is simple for process, cost is low, can produce at any time as required, have overcome difficult extraction in general enzyme source and the costly problem in enzyme source.The Cordyceps polysaccharide that obtains according to aforesaid method has the particularly immune antitumor action of pharmacological action widely, its anti-tumor activity mainly relies on host's reaction, that is to say that it produces antibody by activation phagocytic cell and stimulation and reaches the physiological function that improves the human immunological competence, after antibody produces, just show as intensive anti-tumor activity and the restraint very strong to cancer cells, add the effect of cordycepin and organoselenium, its drug effect is relatively extensively also practical, thereby we can say that it is prevention and the functional matrix for the treatment of all diseases.
The production technique of product of the present invention is carried out biotechnology production fully; do not adopt any chemical substance; the starting material of using also all derive from agricultural byproducts; do not produce any source of pollution; the product accessory that obtains has the rare Chinese medicine material of high added value and advanced rare biochemical special effect medicine raw material both at home and abroad; with high content of technology, competitive power is strong, market outlook good; the practicing of its technical scheme can effectively be alleviated the contradiction of protection environment and human's demand, and realizes that manually instruction and batch production production surrogate have been founded precedent for rare, natural crude drugs for China.
Claims (6)
1, a kind of method that is used to produce Cordyceps fungus powder is characterized in that comprising following production process:
1.1, the acquisition of good pure bacterial strain bacterial classification: the fresh individuality that selects the natural cordyceps sporophore, from bacterial strain, filter out the bacterial strain of relatively stable and better performances with the primary dcreening operation of routine and multiple sieve method, then successively by the isolating method acquisition of conventional microfloras such as separation, ultraviolet mutagenesis, rejuvenation and contrast peacilomyce hepiahi bacterium kind;
1.2, with peacilomyce hepiahi bacterium kind fermentative Production winter worm summer herb thallus: comprise two kinds of solid fermentation method and solution fermentations:
1.2a, the solid fermentation working system---making the bacterial classification that obtains through 1.1 operations is to inoculate on 6.8～7.2 the comprehensive PDA culture medium at pH value, and makes it to make solid fermentation with substratum and cultivates 15～20 days, culture temperature is 16～25 ℃; Substratum is separated with the growth thalline, and thalline is carried out cryodrying, temperature obtains dry thalline below 60 ℃;
1.2b, the liquid fermenting working system---will insert pH value through the bacterial classification that 1.1 operations obtain is to inoculate in 6.8～7.2 the comprehensive PDA culture medium liquid jar fermenter, and makes it to do submerged fermentation cultivated 5～7 days in liquid medium, culture temperature is 16～25 ℃; Thereafter the method by evaporation concentration and Plate Filtration is separated the thalline after liquid medium and the growth, and isolated thalline is carried out spraying drying, obtains dry thalline;
1.3, pulverize and to produce ultrafine powder: will make granularity through the dry thalline of 1.2 operations acquisition with the cell pulverization machine is-300 purpose ultrafine powders, and envrionment temperature is no more than 60 ℃ in the crushing process.
2, the method for production Cordyceps fungus powder as claimed in claim 1, the prescription that it is characterized in that every part of used in the 1.2a process solid-state comprehensive PDA culture medium is: 20 weight unit of mealy potato, 20 weight unit of sucrose, 1.5 weight unit of potassium primary phosphate, 1 weight unit of sal epsom, a vitaminB10 .02 weight unit, 1.5 weight unit of native protein, 30 weight unit of wheat bran, 10 weight unit of Semen Maydis powder.
3, the method for production Cordyceps fungus powder as claimed in claim 1, the prescription that it is characterized in that per unit part comprehensive PDA culture medium liquid used in the 1.2b process is: mealy potato 20g, sucrose 20g, potassium primary phosphate g, sal epsom 1g, Wei ShengsuB2 0mg, native protein 1.5g, wheat bran 30g, Semen Maydis powder 10g, water 1000ml.
4, a kind of Cordyceps fungus powder is characterized in that the product of said bacterium powder for cell wall breaking rate＞95% that makes according to the described production method of claim 1.
5, a kind of method of extracting active immne Cordyceps polysaccharide lyophilized powder according to the described Cordyceps fungus powder of claim 4, it is characterized in that under being lower than 40 ℃ of conditions, 1 part of proteolytic ferment, 1～2 part of amylolytic enzyme and 1 part of cellulolytic enzyme being added respectively in 10 parts of Cordyceps fungus powders, be under 75% the condition it to be stirred in water content, then be after 25～40 ℃ and pH value are to act on 45～90 minutes under 6.0～8.0 conditions in temperature, adopt conventional organic solvent precipitation method to extract polysaccharide component in the above-mentioned material; Obtaining content by the frozen drying method again is 75～85% polysaccharide raw product; Then utilize proteolytic ferment, amylolytic enzyme and cellulolytic enzyme to extract again with the raw product effect respectively again, and utilize absorption method that extract is again decoloured and handle the back and obtain content at the refinishing polyose product more than 95%, again with after the freeze-drying of refinishing polyose product, obtain active immne Cordyceps polysaccharide lyophilized powder at last.
6, a kind of active immne Cordyceps polysaccharide lyophilized powder is characterized in that the product of said lyophilized powder for making according to claim 1 and 5 described production methods.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856120A (en) * | 2010-03-23 | 2010-10-13 | 无锡市天赐康生物科技有限公司 | Health food for improving immunity of human body and preparation method thereof |
| CN101695257B (en) * | 2009-10-30 | 2011-03-30 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN103169065A (en) * | 2013-03-21 | 2013-06-26 | 武汉蜀泰科技有限公司 | Wall breaking method of cordyceps sinensis |
| CN104620844A (en) * | 2013-11-06 | 2015-05-20 | 孙悦迎 | Method for producing fermentative cordycep fungal powder and cordyceps polysaccharide powder through fermentation technology |
| CN105754809A (en) * | 2015-12-04 | 2016-07-13 | 北京本草通汇科技中心 | Organic selenium-rich original wine with Tibetan cordyceps and method for industrially producing organic selenium-rich original wine |
| WO2019223286A1 (en) * | 2018-05-25 | 2019-11-28 | 江西国药有限责任公司 | Preparation method for fermented cordyceps sinensis powder cs-4 decoction pieces |
-
2003
- 2003-12-11 CN CNA2003101189883A patent/CN1546644A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695257B (en) * | 2009-10-30 | 2011-03-30 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN101856120A (en) * | 2010-03-23 | 2010-10-13 | 无锡市天赐康生物科技有限公司 | Health food for improving immunity of human body and preparation method thereof |
| CN103169065A (en) * | 2013-03-21 | 2013-06-26 | 武汉蜀泰科技有限公司 | Wall breaking method of cordyceps sinensis |
| CN104620844A (en) * | 2013-11-06 | 2015-05-20 | 孙悦迎 | Method for producing fermentative cordycep fungal powder and cordyceps polysaccharide powder through fermentation technology |
| CN105754809A (en) * | 2015-12-04 | 2016-07-13 | 北京本草通汇科技中心 | Organic selenium-rich original wine with Tibetan cordyceps and method for industrially producing organic selenium-rich original wine |
| CN105754809B (en) * | 2015-12-04 | 2019-11-12 | 锬酃藏虫草生物科技(深圳)有限公司 | A kind of method and products therefrom of industrialized production organic Se-rich Tibetan cordyceps original plasm wine |
| WO2019223286A1 (en) * | 2018-05-25 | 2019-11-28 | 江西国药有限责任公司 | Preparation method for fermented cordyceps sinensis powder cs-4 decoction pieces |
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