WO2019223286A1 - Preparation method for fermented cordyceps sinensis powder cs-4 decoction pieces - Google Patents

Abstract

A preparation method for fermented Cordyceps sinensis powder Cs-4 decoction pieces. Said method comprises the following steps: cultivating a strain to obtain a qualified mycelium liquid, collecting the qualified mycelium liquid, filtering and blow-drying same to obtain mycelia, drying the blow-dried mycelia using a double-cone dryer, preparing the dried mycelia into pills, drying the pills in an oven, and crushing, mixing and packaging same to obtain the Cordyceps sinensis powder Cs-4 decoction pieces. The method performs slow multiple drying on the mycelia, and thus well protects the medicinal ingredients in the fermented Cordyceps sinensis powder, and improves the medicinal value of the fermented Cordyceps sinensis powder. In addition, the fermented Cordyceps sinensis powder Cs-4 decoction pieces prepared by the present method have good color and luster, have good fragrance, and are convenient to carry and use.

Description

Preparation method of fermented Cordyceps fungus powder Cs-4 decoction pieces Technical field

The invention relates to the field of pharmaceuticals, in particular to a method for preparing fermented Cordyceps powder Cs-4 decoction pieces.

Background technique

Cordyceps sinensis, also known as Cordyceps sinensis, Cordyceps sinensis, Cordyceps sinensis, is a Cordyceps sinensis fungus larvae parasitic in the alpine meadow soil by the carnivorous genus Cordyceps. In summer, a long rod-shaped constellation is drawn from the head of the zombie, that is, the complex of the fruit body of the Cordyceps sinensis and the sclerotia of the zombie. Cordyceps sinensis has a variety of effects such as regulating the function of the immune system, anti-tumor, anti-fatigue, nourishing lungs and kidneys, hemostasis and phlegm, and nourishing essence and qi. Because its output is decreasing year by year and the demand is increasing year by year, the researchers finally realized the artificial fermentation method to cultivate Cordyceps mycelium through unremitting efforts. The most common method of cultivating Cordyceps mycelium is: obtaining strains, then slant culture, shake flask culture, seed tank culture, and fermentation culture, followed by filtration to obtain mycelium, drying, crushing, sieving, mixing, and packaging to obtain Cordyceps fungus powder .

Existing processes for preparing Cordyceps fungus powder are that the mycelia obtained by filtering are dried, directly crushed, and then mixed to obtain a product. The existing preparation process involves only one drying, and a single drying has various problems. If only drying in an oven, the drying time is too long, and a large amount of stacking will make it difficult to dry the interior thoroughly; if simply drying with a double cone, it is easy to cause the product to "burn" due to poor control of temperature and time.

At present, fermented Cordyceps powder powder pieces have no mature processing technology, resulting in a low utilization rate of originally high-value medicinal materials. Therefore, in order to improve the medicinal value of Cordyceps powder, it is necessary to study a new preparation method of Cordyceps powder, which can protect the original medicinal value of Cordyceps powder.

Summary of the Invention

The purpose of the present invention is to provide a method for preparing fermented Cordyceps fungus powder Cs-4 decoction pieces, improve the fermentation method of bacteria, improve the drying method of mycelium, better protect the original medicinal ingredients of Cordyceps fungus powder, and improve Medicinal value.

Specifically, in view of the shortcomings of the prior art, the present invention provides the following technical solutions:

A method for preparing fermented Cordyceps powder Cs-4 decoction pieces, the method includes the following steps:

S1. Strain culture to obtain qualified mycelial body fluids;

S2. Collect the mycelial body fluid obtained in step S1, filter and blow dry to obtain mycelium;

S3. Use a double cone dryer to dry the mycelium obtained in step S2;

S4. The dried mycelia of step S3 are made into pills;

S5. Drying the pill prepared in step S4;

S6. Crush the dried pill in step S5 to obtain a medicinal powder;

S7. Mix the medicine powder and package to obtain fermented Cordyceps powder Cs-4 decoction pieces.

Double cones dry the mycelium, then make a pill, and continue to dry the pill. This process is to slowly and repeatedly dry the mycelium with high water content after filtering, which can completely dry the mycelium and prevent excessive drying. The active components of the fungus powder were destroyed, which improved the medicinal value of the fermented Cordyceps fungus powder Cs-4.

Preferably, the strain culture in step S1 includes slant culture, mother bottle culture, seed tank culture, propagation tank culture, and fermentation tank culture; wherein the culture medium components of the slant culture include: glucose, peptone, bran, and sulfuric acid. Potassium hydrogen, magnesium sulfate, agar, and water; the culture medium components of the mother bottle culture include: glucose, peptone, bran, potassium dihydrogen phosphate, magnesium sulfate, and water.

Preferably, the medium components of the seed tank culture, the propagation tank culture and the fermentation tank culture all include: soybean meal, glucose, sucrose, soybean oil, potassium dihydrogen phosphate, magnesium sulfate, and water.

Preferably, the filtration in step S2 is selected from one of a plate and frame filtration method, a high-speed centrifugal filtration method, a bag filtration method and a column filtration method, preferably a plate and frame filtration method; the filtration time is 3-5 hours, and the pressure is 0.25-0.35MPa.

Preferably, the jacket pressure of the double cone dryer in step S3 is 0.15-0.25 MPa, the vacuum degree is -0.05Mpa to -0.04Mpa, the temperature is 50-90 ° C, and the drying time is 8-18 hours.

Preferably, the solid content of the mycelium obtained in step S2 is 30-40%.

Preferably, the water content of the mycelium after the double cone drying in step S3 is less than 55%.

Preferably, the solid content of the dried pellets in step S5 is above 85%.

Preferably, the drying in step S5 is drying in an oven, and the storage time of the pills made in step S4 before entering the drying in the oven is within 24 hours.

Preferably, the blow-drying in step S2 uses air with a pressure of 0.1-0.2Mpa and blow-drying for 1-2 hours; the pulverizing temperature in step S6 is 25-28 ° C. and pulverization is performed twice.

Compared with the prior art, the effects and benefits of the present invention are:

(1) Self-made culture medium, gradually expanding the culture of Cordyceps strains, and finally obtaining qualified mycelial body fluid in the fermentation tank, the mycelium concentration is above 15%.

(2) Slow and multiple drying of the filtered mycelium by double cone drying, pill making and oven drying in order, which can well protect the medicinal ingredients in the fermented Cordyceps fungus powder, and improve the fermented Cordyceps fungus powder Cs-4 Medicinal value of decoction pieces.

(3) Through the control of temperature and time during the entire preparation process, the mycelium is slowly formed to obtain the fermented Cordyceps fungus powder Cs-4. The fermented Cordyceps fungus powder Cs-4 has the characteristics of good color, fragrant smell, easy to swallow, and easy to carry. It can be swallowed in water or stewed in soup to meet the needs of different scenarios.

Detailed ways

The preparation method of the fermented Cordyceps fungus powder Cs-4 decoction piece includes the following steps:

S1. Strain culture to obtain qualified mycelial body fluids: Isolate the strain Paccilomyces hepiali Cs-4 from fresh Cordyceps sinensis in Qinghai, first perform slant culture, then select colonies with full mycelium growth, many spores, and no contamination by bacteria. Cultivation, when the strain concentration is above 10%, and the residual sugar, residual nitrogen and pH meet the requirements, transfer to a seed tank for cultivation, and then enter the breeding tank and fermentation tank for cultivation in order to obtain a qualified mycelial body fluid.

S2. Collect the mycelial body fluid in the fermenter and filter to obtain moist mycelium; the filtering method is preferably plate and frame filtration, the filtering time is 3-5 hours, and the pressure is 0.25-0.35 MPa. After the filtration is completed, the mycelium is dried with air at a pressure of 0.1-0.2 MPa for 1-2 hours, and a mycelium with a solid content of 30-40% is obtained.

S3. Use a double cone dryer to dry and dry the mycelium after filtering: the jacket pressure of the dryer is 0.15-0.25MPa, the vacuum is -0.05Mpa to -0.04Mpa, the temperature is 50-90 ° C, and the drying time For 8-18 hours. The moisture content of the dried mycelium is less than 55%.

S4. The mycelium dried by the double cone is made into pills; the prepared pills are evenly packed in a stainless steel tray, and the thickness of the pills in the stainless steel tray is controlled to be 2-3 layers.

S5. Within 24 hours, preferably within 12 hours, the stainless steel tray containing the pills is sent to an oven for drying. The temperature in the oven is 50-90 ° C, the vacuum is -0.04Mpa, the drying time is 18-26 hours, and the solid content of the pills after drying is above 85%.

S6. Crush the pill dried in the oven to obtain a powder; the crushing temperature is 25-28 ° C., and the powder is crushed twice; 95% of the powder obtained through the crushing can pass through a No. 7 sieve (120 mesh).

S7. Mix the medicinal powder under the environment of 25-28 ° C, and then package to obtain the fermented Cordyceps powder Cs-4.

In a preferred embodiment, in terms of w / v (w is the weight of the solid material and v is the total volume of the culture medium), the composition of the slanted culture medium is: glucose 2-5%, peptone 0.2-0.5%, bran 4-10%, potassium dihydrogen phosphate 0.1-0.2%, magnesium sulfate 0.02-0.03%, agar 2-5%, and then add water to the preparation amount (ie, the total volume of the culture medium v). The composition of the mother bottle culture medium is: glucose 2-5%, peptone 0.4-1.0%, bran 4-10%, potassium dihydrogen phosphate 0.1-0.2%, magnesium sulfate 0.02-0.03%, and then water is added to the preparation amount. The conditions for slant culture are: constant temperature of 23 ± 3 ° C, humidity control at 80-95%, and culture for 5-7 days. The conditions of the mother bottle culture are: in a 23 ± 3 ° C constant temperature room, the humidity is controlled to 80-95%, and the culture is carried out on a shaker for 3-4 days.

In another preferred embodiment, the medium in the seed tank, the breeding tank and the fermentation tank is composed of soybean meal, glucose, sucrose, soybean oil, potassium dihydrogen phosphate, magnesium sulfate, and water. The pH is natural and the pH value is controlled. 6.8-7.8, but the proportion of each component in different culture tanks is different. The temperature in the culture tank is 23 ± 6 ° C, the tank pressure is 0.03-0.07Mpa, and the culture time is 50-90 hours.

The present invention is further described below with specific examples.

It should be noted that the materials and equipment used in the present invention are all commercially available; among them, the strain Paccilomyces hepiali Cs-4 was isolated from fresh Cordyceps sinensis in Qinghai, and the strain was deposited in the Chinese microorganism on October 21, 1997. Ordinary Microbiology Center of Strain Preservation Management Committee, deposit number is CGMCC NO.0327.

Example 1

Strain isolation

The fresh Cordyceps sinensis sclerotia were washed, rinsed and disinfected with sterile water several times. Under aseptic conditions, the outer skin was cut off, avoiding the intestinal tract, and the white tissue was cut into thin slices. After 7 days of static culture at ℃, the white hypha can grow from the sheet, and the white hypha can be further separated and purified to obtain the Cordyceps sinensis strain, that is, Cs-4.

Expansion of Cordyceps strain Paccilomyces Hepiali Cs-4 to obtain qualified mycelial body fluids

The strain cultivation process is shown in the following steps. In this embodiment, three batches of Cordyceps strains are expanded and cultured. The composition and culture conditions of the slant culture and mother bottle culture of each batch are shown in Table 1. The amount of each raw material in Table 1 is calculated relative to the total formulated amount (ie, the total volume of the culture medium v).

Table 1. Composition and culture conditions of slant and mother bottle culture

(1) Inclined culture

a. Preparation of slanted media:

Take the weighed bran, boil it with drinking water for half an hour, filter and take the filtrate, add other raw materials, dissolve and dilute it with drinking water to the prepared amount, and then divide it into test tubes, the filling amount is 15-20mL / piece (can also be divided Packed into eggplant-shaped bottles, 60mL / piece), stoppered, wrapped, put into high-pressure steam sterilization in a sterilizer, steam pressure 0.09-0.11Mpa, temperature 121 ℃, sterilization time 30 minutes, after cooling Beveled as required.

b. Inclined inoculation culture:

Take out the stored bacteria, and under sterile conditions, use an inoculation shovel to scoop about 1cm ^{2 of the} original seed, and evenly spread it on the inclined surface. Put a cotton stopper on the bottle stopper and wrap it. 23 ± 3 ℃ constant temperature and humidity control It is cultured at 80-95% for 5-7 days. After the growth is mature, visual inspection is performed. The culture characteristics are: spores and mycelium are plump and white or light yellow fluff. Remove the slanted surface with poor appearance and place the qualified slanted surface in the refrigerator at 1-8 ° C for future use.

(2) Mother bottle culture

a. Preparation of mother bottle culture medium:

Take the weighed bran, boil it with drinking water for half an hour, filter, and take the filtrate. Add other raw materials according to the proportion, dissolve and dilute with drinking water to the preparation amount, and then distribute into the triangle bottle. The 750mL triangle bottle is 200mL, stoppered, wrapped, put into high-pressure steam sterilization in a sterilization cabinet, 0.09-0.11Mpa pressure, 120 ± 1 ℃ steam sterilization for 30 minutes, and wait to cool to less than 25 ℃ Rear use.

b. Mother bottle inoculation culture:

Under aseptic conditions, a 1 cm ² slant cultured mycelium with a shovel for inoculation was used to grow the colony with abundant spores and no bacteria. In a 23 ± 3 ° C constant temperature room, the humidity is controlled at 80-95%, and placed on a shaker and cultured for 3-4 days.

c, and bottle:

Under aseptic conditions, 10-15 vial (750 mL female vials) female vial seeds were combined into inoculated steel vials. Take a small amount of seed solution from the inoculation steel bottle for sterility test, take an amount of a mother bottle for intermediate determination, check the mycelium concentration, residual sugar, residual nitrogen, pH; after inspection, no bacteria, bacterial concentration ≥ 10%, intermediate The measurement meets the requirements, and the pressure difference method is used to access the seed tank.

(3) Seed tank, breeding tank and fermentation tank culture

Based on the total volume of the culture medium v, the solid material ratio is w / v, and the liquid material is calculated according to v / v. The composition and culture conditions of the culture medium in each culture tank are shown in Table 2. The inoculation methods are differential pressure method. Three batches of strains were cultured in the same conditions.

Table 2. Medium composition and culture conditions of seed tank, breeding tank and fermentation tank

When the bacteria concentration in the seed tank is ≥8% and there are no foreign bacteria, inoculate it into the breeding tank for cultivation; when the bacteria concentration in the breeding tank is ≥8% and there is no foreign bacteria, inoculate it into the fermentation tank; the bacteria concentration in the fermentation tank is ≥ 15% and no bacteria, that is, qualified mycelial body fluid.

In this embodiment, three batches of qualified Cordyceps mycelium body fluids are prepared, which are mycelial body fluid ①, mycelial body fluid ②, and mycelial body fluid ③; the prepared mycelial body fluid can be used to prepare Cordyceps fungus powder Cs-4 decoction pieces.

Example 2

Preparation of fermented Cordyceps fungus powder Cs-4 decoction pieces

Collect the qualified mycelial body fluid from the fermentation tank ①, and pump the mycelial body fluid into a plate and frame filter with a pump for filtration. The filtration pressure was controlled to 0.35 MPa, and the filtration time was 4 hours. After the filtration was completed, the mycelium obtained by the filtration was air-dried for 2 hours at an ambient temperature of 26 ° C. and an air-drying pressure of 0.2 MPa, to obtain a mycelium with a solid content of 35%.

The blown mycelium was put into a double cone dryer, and the vacuum and steam valves were opened for vacuum drying. The jacket pressure was 0.25 MPa, the vacuum was -0.04Mpa, the temperature was 65 ° C, and the drying was performed for 10 hours. After 8 hours of drying, take a non-stick sampling shovel every half an hour for moisture detection. When the moisture of the bacteria is less than 55%, it can be taken out.

Take the double cone drying mycelium with qualified quality, use a medicine shovel to add the double cone drying mycelium to the hopper of the extruder pill making machine, turn on the extruder pill making machine switch, and perform pelleting. The obtained pills are evenly packed. Into the stainless steel tray, control the thickness of the pills in the stainless steel tray to 2 layers. After 4 hours of storage, the stainless steel tray containing the pills was placed in an oven, and the vacuum and steam were used to dry it; the vacuum was -0.04Mpa, the temperature was 65 ° C, and the drying time was 20 hours. The bacteria with a solid content of 85% were obtained. Silk body products.

The pellets after the drying are slowly put into the coarse pulverizer, and pulverized again after the first pulverization is completed, and the pulverization temperature is 25 ° C; the pulverized cordyceps powder can pass through the No. 7 medicine sieve. Then, the pulverized bacterial powder was put into the cleaned two-dimensional mixer, and mixed at a controlled room temperature of 28 ° C. and normal pressure for 30 minutes. The mixed fungal powder is packaged in a sterile environment to prepare a finished product of fermented Cordyceps fungus powder Cs-4. The powder of the fermented Cordyceps fungus powder Cs-4 prepared by the method has fine powder, good color and pleasant fragrance.

Example 3

Preparation of fermented Cordyceps fungus powder Cs-4 decoction pieces

Collect the qualified mycelial body fluid from the fermentation tank ②, and pump the mycelial body fluid into a plate and frame filter with a pump for filtration. The filtration pressure was controlled to 0.30 MPa, and the filtration time was 5 hours. After the filtration was completed, the mycelium obtained by the filtration was air-dried for 2 hours at an ambient temperature of 26 ° C and an air-drying pressure of 0.1 MPa, to obtain a mycelium with a solid content of 30%.

The blown mycelium was put into a double cone dryer, and the vacuum and steam valves were opened for vacuum drying. The jacket pressure was 0.15 MPa, the vacuum degree was -0.05Mpa, the temperature was 50 ° C, and the drying time was 18 hours. After drying for 15 hours, take a non-stick sampling shovel every half an hour for moisture detection. When the moisture of the bacteria is less than 55%, you can take it out.

Take the double cone drying mycelium with qualified quality, use a medicine shovel to add the double cone drying mycelium to the hopper of the extruder pill making machine, turn on the extruder pill making machine switch, and perform pelleting. The obtained pills are evenly packed. Into the stainless steel tray, control the thickness of the pills in the stainless steel tray to 3 layers. After 12 hours of storage, put the stainless steel tray containing the pills into the oven, and turn on the vacuum and steam to dry; the vacuum degree is -0.04Mpa, the temperature is 90 ° C, and the drying time is 18 hours, and the bacteria with a solid content of 88% are obtained. Silk body products.

The pellets after the drying are slowly put into the coarse pulverizer, and pulverized again after the first pulverization is completed, and the pulverization temperature is 27 ° C; 95% of the pulverized Cordyceps powder can pass through the No. 7 medicine sieve. Then, the pulverized bacterial powder was put into the cleaned two-dimensional mixer, and mixed at a controlled room temperature of 25 ° C. and normal pressure for 30 minutes. The mixed fungal powder is packaged in a sterile environment to prepare a finished product of fermented Cordyceps fungus powder Cs-4. The powder of the fermented Cordyceps fungus powder Cs-4 prepared by the method has fine powder, good color and pleasant fragrance.

Example 4

Preparation of fermented Cordyceps fungus powder Cs-4 decoction pieces

Collect the qualified mycelial body fluid from the fermentation tank ③, and pump the mycelial body fluid into a plate and frame filter with a pump for filtration. Control the filtration pressure to 0.25 MPa and the filtration time to 3 hours. After the filtration was completed, the mycelium obtained by the filtration was air-dried for 1 hour at an ambient temperature of 26 ° C. and an air-drying pressure of 0.2 MPa to obtain a mycelium with a solid content of 40%.

The blown mycelium was put into a double cone dryer, and the vacuum and steam valves were opened for vacuum drying. The jacket pressure was 0.20 MPa, the vacuum was -0.04 MPa, the temperature was 90 ° C, and the drying time was 8 hours. After 5 hours of drying, take a non-stick sampling shovel every half an hour for moisture detection. When the moisture of the bacteria is less than 55%, it can be taken out.

Take the double cone drying mycelium with qualified quality, use a medicine shovel to add the double cone drying mycelium to the hopper of the extruder pill making machine, turn on the extruder pill making machine switch, and perform pelleting. The obtained pills are evenly packed. Into the stainless steel tray, control the thickness of the pills in the stainless steel tray to 2 layers. After storing for 24 hours, put the stainless steel tray containing the pills into the oven, and turn on the vacuum and steam to dry; the vacuum degree is -0.04Mpa, the temperature is 50 ° C, and the drying time is 26 hours. The bacteria with a solid content of 90% are obtained. Silk body products.

The pills after the above drying are slowly put into the coarse pulverizer, and the pulverization is performed again after the first pulverization is completed, and the pulverization temperature is 28 ° C; 95% of the pulverized Cordyceps powder can pass through the No. 7 medicine sieve. Then, the pulverized bacterial powder was put into a cleaned two-dimensional mixer, and mixed at a controlled room temperature of 26 ° C. and normal pressure for 30 minutes. The mixed fungal powder is packaged in a sterile environment to prepare a finished product of fermented Cordyceps fungus powder Cs-4. The powder of the fermented Cordyceps fungus powder Cs-4 prepared by the method has fine powder, good color and pleasant fragrance.

Comparative Example 1

The mycelial body fluid ① was prepared by the same method as in Example 1, and then continued processing to prepare fermented Cordyceps powder Cs-4 decoction pieces. The process of preparing decoction pieces in this comparative example is as follows:

Put the mycelial body fluid into the plate and frame filter artificially, and filter it at 0.35MPa for 3 hours; then put the filtered mycelium into the double cone dryer to dry, the jacket pressure is 0.25MPa, and the vacuum degree is -0.04MPa, drying at 65 ℃ for 28 hours. After drying, crush at room temperature, mix the materials, and package to obtain the fermented Cordyceps fungus powder Cs-4. The particles of Cordyceps powder prepared by this method are not uniform, and only 50% of the powder passed through the No. 7 sieve. In addition, the dried material can be seen to be charred, which results in the color of Cordyceps powder Cs-4 decoction pieces. First, it affects medicinal value.

Comparative Example 2

The mycelial body fluid ② was prepared by the same method as in Example 1, and then continued processing to prepare fermented Cordyceps powder Cs-4 decoction pieces. The process of preparing decoction pieces in this comparative example is as follows:

The mycelial body fluid was put into a plate and frame filter with a pump, and filtered at 0.30 MPa for 5 hours; then, the wet mycelial body fluid obtained by filtration was blown dry with air at 0.1 MPa for a total of 2 hours. Next, the blown-out mycelium was dried in an oven with a vacuum degree of -0.04 MPa, and dried at 90 ° C for 32 hours. After drying, crush at room temperature, mix the materials, and package to obtain the fermented Cordyceps fungus powder Cs-4. The particles of Cordyceps powder prepared by this method are not uniform due to insufficient drying and agglomeration. Eventually, the color of the fermented Cordyceps fungus powder Cs-4 will vary, affecting its medicinal value.

Comparative Example 3

The mycelial body fluid was prepared in the same manner as in Example 1 and then continued to prepare fermented Cordyceps powder Cs-4 decoction pieces. The process of preparing decoction pieces in this comparative example was similar to that in Example 4, but no preparation was performed after the first drying. Into pills. This comparative example is also different from Example 4 in that the mycelial body fluid is manually put into the plate and frame filter, and the wet mycelium is directly put into the double-cone dryer for drying after the filtration is completed. The temperature was 95 ° C and the degree of vacuum was -0.03 MPa. After the drying of the double cone, it was left for 2 days, then placed in an oven for drying, and finally crushed, mixed, and packaged to obtain fermented Cordyceps fungus powder Cs-4 decoction pieces. The granules of Cordyceps powder prepared by this method are not uniform, and there is a phenomenon of agglomeration. Eventually, the color of the fermented Cordyceps powder Cs-4 is different, which affects its medicinal value.

By comparing the product characteristics of the fermented Cordyceps powder Cs-4 decoction pieces prepared in the above Examples 2-4 and Comparative Examples 1-3, it can be clearly obtained that the fermented Cordyceps powder Cs-4 decoction pieces prepared by the method of the present invention better quality. The method of the invention has simple operation, is suitable for industrialized production, and has high practical value.

Example 5

The nutritional analysis was performed on the fermented Cordyceps powder Cs-4 prepared in Example 2-4, and it was found that the Cordyceps powder Cs-4 prepared pieces contained 19 amino acids, of which seven were essential amino acids that cannot be synthesized by the human body. Powdered tablets also contain potassium, sodium, calcium, magnesium, iron, copper, manganese, zinc, phosphorus, selenium, vitamins B1, B2, and vitamin E. The amino acids contained in the fermented Cordyceps powder Cs-4 prepared by the method of the present invention are shown in Table 3.

Amino acid	mg / 100mg sample	Amino acid	mg / 100mg sample
ASP aspartic acid	2.13	LEU Leucine	1.66
THR threonine	1.31	TYR tyrosine	0.80
SER serine	1.06	PHE phenylalanine	1.00
GLU glutamic acid	2.43	LYS Lysine	1.38
GLY glycine	1.12	NH 3 ammonia	0.44
ALA Alanine	1.84	HIS Histidine	0.49
VAL Valine	1.33	ARG arginine	1.29
MET methionine	0.30	PRO Proline	1.37
ILE isoleucine	1.22	TRP tryptophan	0.23
CYS cystine	0.30	sum	21.70

Table 3. Amino acid content in fermented Cordyceps powder Cs-4 prepared by the method of the present invention

The above are only the preferred embodiments of the present invention, but the scope of protection of the present invention is not limited to this. Any person skilled in the art can easily think of changes or changes within the technical scope disclosed by the present invention. Replacement should all be covered by the protection scope of the present invention.

Claims (10)

1. A method for preparing fermented Cordyceps fungus powder Cs-4 decoction pieces is characterized in that the method includes the following steps:

   S1. Strain culture to obtain qualified mycelial body fluids;

   S2. Collect the mycelial body fluid obtained in step S1, filter and blow dry to obtain mycelium;

   S3. Use a double cone dryer to dry the mycelium obtained in step S2;

   S4. The dried mycelia of step S3 are made into pills;

   S5. Drying the pill prepared in step S4;

   S6. Crush the dried pill in step S5 to obtain a medicinal powder;

   S7. Mix the medicine powder and package to obtain fermented Cordyceps powder Cs-4 decoction pieces.
2. The preparation method according to claim 1, characterized in that the strain culture in step S1 comprises slant culture, mother bottle culture, seed tank culture, breeding tank culture and fermentation tank culture; wherein, the culture medium component of the slant culture Including: glucose, peptone, bran, potassium dihydrogen phosphate, magnesium sulfate, agar, and water; the culture medium components of the mother bottle culture include: glucose, peptone, bran, potassium dihydrogen phosphate, magnesium sulfate, and water.
3. The preparation method according to claim 2, characterized in that the medium components of the seed tank culture, the propagation tank culture and the fermentation tank culture all include: soybean cake powder, glucose, sucrose, soybean oil, potassium dihydrogen phosphate, magnesium sulfate And water.
4. The preparation method according to claim 1, wherein the filtration in step S2 is selected from one of a plate and frame filtration method, a high-speed centrifugal filtration method, a bag filtration method and a column filtration method, and preferably a plate and frame filtration method. The filtration time is 3-5 hours and the pressure is 0.25-0.35 MPa.
5. The preparation method according to any one of claims 1 to 4, characterized in that the jacket pressure of the double cone dryer in step S3 is 0.15-0.25 MPa, the vacuum degree is -0.05Mpa to -0.04Mpa, and the temperature is 50-90 ° C, drying time is 8-18 hours.
6. The method according to claim 5, wherein the solid content of the mycelium obtained in step S2 is 30-40%.
7. The preparation method according to claim 5, wherein the water content of the mycelium after the double cone drying in step S3 is less than 55%.
8. The preparation method according to claim 5, wherein the solid content of the pill after drying in step S5 is 85% or more.
9. The preparation method according to claim 5, wherein the drying in step S5 is drying in an oven, and the storage time of the pills made in step S4 before entering the drying in the oven is within 24 hours.
10. The preparation method according to claim 5, characterized in that the blow drying in step S2 uses air with a pressure of 0.1-0.2Mpa and blow drying for 1-2 hours; the crushing temperature in step S6 is 25- Crushed twice at 28 ° C.