CN101736055A - Method for extracting auricularia auricula polysaccharides - Google Patents
Method for extracting auricularia auricula polysaccharides Download PDFInfo
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- CN101736055A CN101736055A CN200810162120A CN200810162120A CN101736055A CN 101736055 A CN101736055 A CN 101736055A CN 200810162120 A CN200810162120 A CN 200810162120A CN 200810162120 A CN200810162120 A CN 200810162120A CN 101736055 A CN101736055 A CN 101736055A
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Abstract
The invention relates to a method for extracting auricularia auricula polysaccharides, which comprises the following processes: soaking; adding complex enzyme and prolease, and extracting; taking supernate through centrifuging; obtaining a crude product through ethanol precipiration; refining the crude product, and the like. The extracting method is simple and convenient; and the extracted auricularia auricula polysaccharides contain three polysaccharides, namely crude polysaccharide (FA), acid polysaccharide (FA-A) and neutral polysaccharide (FA-N). The extracted auricularia auricula polysaccharides are prepared into proper medicaments for resisting ischemic cardiovascular and cerebrovascular diseases clinically, are favorable for further developing medicaments and foods related to the auricularia auricula polysaccharides, improve processing added value of black fungus and greatly improve the economic benefit of the black fungus.
Description
Technical field
The present invention relates to a kind of extracting method of Auricularia polycose.
Background technology
Black fungus has another name called wooden moth, also the name wooden chicken, the tree chicken, it is one of principal item of edible mushrooms, has very high nutritive value.Black fungus also has higher medicinal effect except edible in the people life.Ming Dynasty's LI Shi-Zhen has shown effects such as black fungus has cool blood, invigorates blood circulation, beneficial gas is kept fit to the record of black fungus " beneficial gas is not hungry, the strong will of making light of one's life by commiting suicide, disconnected paddy is controlled hemorrhoid " in Compendium of Material Medica.Black fungus contains abundant colloid, has good clear sliding effect for Digestive tract.Along with people to black fungus pharmaceutical use understanding gradually deeply, find that black fungus has biologic activity widely, wherein the effect of cardiovascular and cerebrovascular is caused just gradually people's attention.Wherein, from known pharmacotoxicological effect as can be known, Auricularia polycose is blood fat reducing, cholesterol, blood sugar and lipofuscin content obviously, also have effects such as antitumor, anti-inflammatory, anticoagulation, atherosclerosis, anti-hemocyte reduce, anti-radiation, antithrombotic formation simultaneously, but and enhance SOD vigor, reduce the objectionable impurities free radical and produce and also improve immunizing power.
The history in existing more than 2000 year of the record of black fungus.The growth distribution of wild black fungus is very wide, mainly is distributed in geographic Northeast Asia, temperate zone, the Northern Hemisphere, and China is with a vast territory, and most of area belongs to thermal zone, has a moderate climate, and is with heavy rainfall, the growing of very suitable black fungus.Therefore, China be produce the earliest in the world and the northern area output of country, especially China of edible black fungus maximum.Along with the development of continuous progress in science and technology and economic society, the variation of essence has also taken place in the growth pattern of black fungus and distribution, from grows wild method one linden punching inoculation method-cultivating in bag method.Mainly be distributed in ground such as Liaoning, Jilin, Heilungkiang, Sichuan, Zhejiang, Hebei, Fujian at present, Zhejiang Province is the main producing region with the Lishui.Ton surplus the bright black fungus output in Lishui reached 40,000 in 2004 is one of major source of revenues of peasants such as scape Ning County, Longquan City, Qingyuan County, and resource is very abundant, but its product processing added value less than 10%.Lishui College and Zhejiang University's medical research group are found in the screening study to Auricularia polycose recently, Auricularia polycose is irritated and can be reduced arteria cerebri media blocking-up, general character focal cerebral ischemia, the multiple infarction of brain area that causes of irritating again in one week of stomach, improve neural function scoring, alleviate the cardiomyocyte cell death that global ischemia causes and the release of cell injury enzyme.This preliminary observation shows that Auricularia polycose has the pharmacological effect of potential antagonism ischemic cardio cerebrovascular diseases.Therefore, Auricularia polycose may have higher medicinal and edibleness, further processes and develops relevant medicine of Auricularia polycose and food and have bigger social benefit and economic worth.And yet there are no the relevant report of Auricularia polycose proposition method now.
Summary of the invention
In order to improve the processing added value of black fungus product, further develop medicine and the food relevant with Auricularia polycose, the purpose of this invention is to provide the extracting method of the easy Auricularia polycose of a kind of method.
To achieve the above object, the present invention has adopted following technical scheme: a kind of extracting method of Auricularia polycose comprises following technology:
A) take by weighing black fungus, pulverize after, add the water of 80～100 times of weight ratios, soaked 20～40 minutes;
B) add composite hydrolysis enzyme reaction 20～40 minutes, temperature of reaction remains on 40～60 degrees centigrade;
C) add papoid reaction 80～100 minutes again, temperature of reaction keeps 40～60 degrees centigrade again;
D) enzyme that in 80～100 degrees centigrade of water-baths, goes out, lixiviate 1～2 hour, extract;
E) get above-mentioned steps d) the black fungus extract, with centrifugal 5～15 minutes of whizzer, remove precipitation, concentrated supernatant;
F) get above-mentioned supernatant liquor, being 90～95% ethanol with concentration of volume percent precipitates by 1: 2～1: 4 volume ratio, the Auricularia polycose crude product;
G) it is multiple water-soluble to get above-mentioned crude product, adds and 1: 3 by volume～1: 5 Sevag reagent deproteinated of sample liquid;
H) concentrate after the supernatant with dense volume percent degree 90～95% ethanol sedimentations, drying, must elaboration.
As preferably, above-mentioned steps b) composite hydrolytic enzyme in contains cellulase and polygalacturonase.
As preferably, above-mentioned every gram cellulase contains the 40000 enzymes unit that lives.
As preferably, above-mentioned every gram polygalacturonase contains the 20000 enzymes unit that lives.
As preferably, the mass percent concentration of above-mentioned composite hydrolytic enzyme is 2%.
As preferably, above-mentioned every gram papoid contains the 250000 enzymes unit that lives.
As preferably, the mass percent concentration of above-mentioned papoid is 1.25%.
As preferably, above-mentioned alcoholic acid concentration of volume percent is 95%.
Excellent as selecting, above-mentioned step h) drying is a vacuum lyophilization.
Simple and convenient extraction of the present invention, the Auricularia polycose that extracts contain 3 kinds of polysaccharide components, comprise Crude polysaccharides (FA), acidic polysaccharose (FA-A) and neutral polysaccharide (FA-N).The Auricularia polycose that the present invention extracts is made as the suitable medicament of ischemia resisting cardiovascular and cerebrovascular diseases medicament clinically, help further exploitation medicine and the food relevant with Auricularia polycose, improved the processing added value of black fungus product, improve the economic benefit of black fungus product greatly, according to budget, Lishui of Zhejiang city scape Ning County black fungus raw materials cost is 60 yuan of per kilograms, adds the cost of extraction, below 10 yuan, the added value of black fungus will improve more than 80 times every gram cost again.
Embodiment
Embodiment: a kind of extracting method of Auricularia polycose comprises following technology:
1, after black fungus 5g, the pulverizing, adds the water of 450g, soaked 30 minutes;
2, add mass percent concentration 2% composite hydrolytic enzyme, reacted 30 minutes, temperature of reaction keeps 50 degrees centigrade again, and wherein composite hydrolytic enzyme contains cellulase and polygalacturonase, and every gram cellulase contains 40,000 enzymes unit alive, and every gram polygalacturonase contains 20,000 enzymes unit alive;
3, add mass percent concentration 1.25% papoid reaction 90 minutes again, temperature of reaction keeps 50 degrees centigrade again; Wherein every gram papoid contains 250,000 enzymes unit alive;
4, again in 90 degrees centigrade of water-bath kinds enzyme that goes out, lixiviate 1.5 hours, extract;
5, get the black fungus extract of above-mentioned steps 4, use centrifugal 10 minutes of whizzer, remove precipitation, concentrate supernatant;
6, get above-mentioned supernatant liquor, precipitate, get the Auricularia polycose crude product with the volume ratio of concentration of volume percent 95% ethanol by 1: 3;
7, it is multiple water-soluble to get above-mentioned crude product, and adding with sample liquid is 1: 4 Sevag reagent deproteinated by volume;
8, concentrate after the supernatant with concentration of volume percent 95% ethanol sedimentation, put into the vacuum freeze drier drying, must elaboration.
The above-mentioned elaboration that makes is a canescence floc sedimentation shape solid, fluffy softness, and the interim time spent adds mass percent concentration 0.9% physiological saline or the dissolving of K-H liquid.
Claims (9)
1. the extracting method of an Auricularia polycose is characterized in that comprising following technology:
A) take by weighing black fungus, pulverize after, add the water of 80～100 times of weight ratios, soaked 20～40 minutes;
B) add composite hydrolysis enzyme reaction 20～40 minutes, temperature of reaction remains on 40～60 degrees centigrade;
C) add papoid reaction 80～100 minutes again, temperature of reaction keeps 40～60 degrees centigrade again;
D) enzyme that in 80～100 degrees centigrade of water-baths, goes out, lixiviate 1～2 hour, extract;
E) get above-mentioned steps d) the black fungus extract, with centrifugal 5～15 minutes of whizzer, remove precipitation, concentrated supernatant;
F) get above-mentioned supernatant liquor, being 90～95% ethanol with concentration of volume percent precipitates by 1: 2～1: 4 volume ratio, the Auricularia polycose crude product;
G) it is multiple water-soluble to get above-mentioned crude product, adds and 1: 3 by volume～1: 5 Sevag reagent deproteinated of sample liquid;
H) use concentration of volume percent 90～95% ethanol sedimentations, drying after the concentrated supernatant, get elaboration.
2. the extracting method of a kind of Auricularia polycose according to claim 1 is characterized in that, the composite hydrolytic enzyme in the described step b) contains cellulase and polygalacturonase.
3. the extracting method of a kind of Auricularia polycose according to claim 2 is characterized in that, described every gram cellulase contains 40,000 enzymes unit alive.
4. the extracting method of a kind of Auricularia polycose according to claim 2 is characterized in that, described every gram polygalacturonase contains 20,000 enzymes unit alive.
5. according to the extracting method of claim 1 or 2 or 3 or 4 described a kind of Auricularia polycoses, it is characterized in that the mass percent concentration of described composite hydrolytic enzyme is 2%.
6. according to the extracting method of claim 1 or 2 or 3 or 4 described a kind of Auricularia polycoses, it is characterized in that described every gram papoid contains 250,000 enzymes unit alive.
7. the extracting method of a kind of Auricularia polycose according to claim 6 is characterized in that, the mass percent concentration of described papoid is 1.25%.
8. according to the extracting method of claim 1 or 2 or 3 or 4 described a kind of Auricularia polycoses, it is characterized in that described alcoholic acid concentration of volume percent is 95%.
9. according to the extracting method of claim 1 or 2 or 3 or 4 described a kind of Auricularia polycoses, it is characterized in that described step h) drying be vacuum lyophilization.
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| CN200810162120A CN101736055A (en) | 2008-11-24 | 2008-11-24 | Method for extracting auricularia auricula polysaccharides |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150822A (en) * | 2010-12-24 | 2011-08-17 | 天津市诺奥科技发展有限公司 | Fresh tree fungus hydrolyzate prepared by utilizing biological enzyme technology |
| CN102150821A (en) * | 2010-12-24 | 2011-08-17 | 天津市诺奥科技发展有限公司 | Method for preparing fresh dried mushroom hydrolysate by bio-enzyme technology |
| CN102585032A (en) * | 2012-03-09 | 2012-07-18 | 武汉大学 | Water-soluble polysaccharide extracted from black fungus and preparation method thereof |
| CN103641924A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of Auricularia auricula polysaccharide from Auricularia auricula |
| CN103932039A (en) * | 2014-04-30 | 2014-07-23 | 河南工业大学 | Nutrient enriched steamed bun prepared from auricularia auricula polysaccharides through enzyme extraction |
| CN104382929A (en) * | 2014-11-25 | 2015-03-04 | 高秀媛 | Medicine for removing blood garbage and vivotoxin and preparation method thereof |
| CN106589156A (en) * | 2016-12-15 | 2017-04-26 | 昆明旭日丰华农业科技有限公司 | Agaric polysaccharide extraction method |
| CN106749724A (en) * | 2016-12-09 | 2017-05-31 | 昭平县科学技术指导站 | A kind of method for extracting Auricularia polysaccharide |
| CN107267571A (en) * | 2017-07-15 | 2017-10-20 | 合肥市晶谷米业有限公司 | A kind of extracting method of rice bran polysaccharide |
| CN108703285A (en) * | 2018-05-22 | 2018-10-26 | 大连工业大学 | A kind of edible fungi polysaccharide compounding high concentration protein beverage and preparation method thereof |
| CN110642959A (en) * | 2019-10-17 | 2020-01-03 | 吉林农业大学 | Auricularia fuscosuccinea acidic polysaccharide, and preparation method and application thereof |
| CN111234044A (en) * | 2020-01-19 | 2020-06-05 | 广西中医药大学 | Low-molecular-weight tremella aurantialba glucuronic acid-xylan and preparation method and application thereof |
-
2008
- 2008-11-24 CN CN200810162120A patent/CN101736055A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150822A (en) * | 2010-12-24 | 2011-08-17 | 天津市诺奥科技发展有限公司 | Fresh tree fungus hydrolyzate prepared by utilizing biological enzyme technology |
| CN102150821A (en) * | 2010-12-24 | 2011-08-17 | 天津市诺奥科技发展有限公司 | Method for preparing fresh dried mushroom hydrolysate by bio-enzyme technology |
| CN102585032A (en) * | 2012-03-09 | 2012-07-18 | 武汉大学 | Water-soluble polysaccharide extracted from black fungus and preparation method thereof |
| CN103641924B (en) * | 2013-11-27 | 2016-03-16 | 威海市桢昊生物技术有限公司 | A kind of method extracting Auricularia polysaccharide from auricularia auriculajudae |
| CN103641924A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of Auricularia auricula polysaccharide from Auricularia auricula |
| CN103932039A (en) * | 2014-04-30 | 2014-07-23 | 河南工业大学 | Nutrient enriched steamed bun prepared from auricularia auricula polysaccharides through enzyme extraction |
| CN104382929A (en) * | 2014-11-25 | 2015-03-04 | 高秀媛 | Medicine for removing blood garbage and vivotoxin and preparation method thereof |
| CN106749724A (en) * | 2016-12-09 | 2017-05-31 | 昭平县科学技术指导站 | A kind of method for extracting Auricularia polysaccharide |
| CN106589156A (en) * | 2016-12-15 | 2017-04-26 | 昆明旭日丰华农业科技有限公司 | Agaric polysaccharide extraction method |
| CN107267571A (en) * | 2017-07-15 | 2017-10-20 | 合肥市晶谷米业有限公司 | A kind of extracting method of rice bran polysaccharide |
| CN108703285A (en) * | 2018-05-22 | 2018-10-26 | 大连工业大学 | A kind of edible fungi polysaccharide compounding high concentration protein beverage and preparation method thereof |
| CN110642959A (en) * | 2019-10-17 | 2020-01-03 | 吉林农业大学 | Auricularia fuscosuccinea acidic polysaccharide, and preparation method and application thereof |
| CN111234044A (en) * | 2020-01-19 | 2020-06-05 | 广西中医药大学 | Low-molecular-weight tremella aurantialba glucuronic acid-xylan and preparation method and application thereof |
| CN111234044B (en) * | 2020-01-19 | 2021-04-27 | 广西中医药大学 | Low-molecular-weight tremella aurantialba glucuronic acid-xylan and preparation method and application thereof |
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Application publication date: 20100616 |