CN1544356A - Probiotic bacterium compound formulation preparation method - Google Patents
Probiotic bacterium compound formulation preparation method Download PDFInfo
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- CN1544356A CN1544356A CNA2003101123462A CN200310112346A CN1544356A CN 1544356 A CN1544356 A CN 1544356A CN A2003101123462 A CNA2003101123462 A CN A2003101123462A CN 200310112346 A CN200310112346 A CN 200310112346A CN 1544356 A CN1544356 A CN 1544356A
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- bacteria
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- compound cultivation
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- 241000894006 Bacteria Species 0.000 title claims abstract description 47
- 150000001875 compounds Chemical class 0.000 title claims description 24
- 239000006041 probiotic Substances 0.000 title claims description 12
- 230000000529 probiotic effect Effects 0.000 title claims description 12
- 235000018291 probiotics Nutrition 0.000 title claims description 12
- 238000009472 formulation Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 238000002360 preparation method Methods 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 claims abstract description 22
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 19
- 239000002131 composite material Substances 0.000 claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 13
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 11
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 11
- 239000010865 sewage Substances 0.000 claims abstract description 9
- 238000012216 screening Methods 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims abstract description 4
- 230000004151 fermentation Effects 0.000 claims abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 16
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 241000233866 Fungi Species 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 229920002472 Starch Polymers 0.000 claims description 10
- 239000008107 starch Substances 0.000 claims description 10
- 235000019698 starch Nutrition 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 7
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 7
- 235000013379 molasses Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 210000000969 egg white Anatomy 0.000 claims description 3
- 235000014103 egg white Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- ILRLTAZWFOQHRT-UHFFFAOYSA-N potassium;sulfuric acid Chemical compound [K].OS(O)(=O)=O ILRLTAZWFOQHRT-UHFFFAOYSA-N 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 2
- 241000235342 Saccharomycetes Species 0.000 abstract 2
- 230000003044 adaptive effect Effects 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 239000004310 lactic acid Substances 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 230000006978 adaptation Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 5
- 241000282414 Homo sapiens Species 0.000 description 4
- 238000000746 purification Methods 0.000 description 3
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 230000007483 microbial process Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A process for preparing healthy composite bacteria, characterized in that photosynthetic bacteria, saccharomycete, pachydermatis and lactic acid bacteria are used as basic bacterial, and the process comprises the steps of, planting these bacterial into organic sewage environment whose COD concentration is greater than 400 for adaptation acclimation, screening dominant production bacterial strain adaptive to high COD and BOD environment, subjecting saccharomycete and photosynthetic bacteria to composite culture, subjecting culturing liquid and pachydermatis to composite culture, and subjecting composite culturing liquid and lactic acid bacteria to composite culture, repeating the above steps to screen and confirm production bacterial strain adaptive to composite environment, on the basis of which a three-step method for fermentation manufacturing technique and culture medium formula can be determined.
Description
Technical field:
The invention discloses a kind of manufacture method of probiotic bacterium composite fungus agent, particularly the manufacture method of environmental protection tailored version probiotic bacterium composite fungus agent.
Background technology:
As everyone knows, the earth of living at us exists countless microorganisms, from the air to the seabed, from the South Pole and equator, from human body, animal, ubiquitous in the body of plant to body surface, microorganism is the most ancient on the earth, the primeval life type that quantity is maximum, be to keep the of paramount importance role of the eubiosis on the earth, there is not the existence of microorganism, just there is not human living space on the earth, the purification of rivers, the regeneration of rubbish even the corruption of corpse, all be the result of microbial process, but along with the progress and the industrialized development of human society, the primary eubiosis is destroyed gradually, with regard to by water surrounding, a large amount of sanitary sewages, breeding wastewater, trade effluent, agricultural effluent is directly arranged the original microorganism structure that people's rivers have destroyed rivers, destroyed the self-purification capacity of water body, made water resources be subjected to serious pollution, these problems have directly jeopardized human beings'health and safety.
Summary of the invention:
The purpose of this invention is to provide and a kind ofly can reduce organic pollutant content, the BOD, the COD that reduce sewage pollute, and recover the rivers self-purification capacity, safely and efficiently the probiotic bacterium composite fungus agent.
The object of the present invention is achieved like this: at first be to select photosynthetic bacteria, yeast, spore bacteria and milk-acid bacteria are basic bacterial classification, these bacterial classifications are implanted COD concentration respectively carry out the adaptability domestication greater than 400 organic sewage environment, preliminary screening goes out each bacterial classification and adapts to high COD, the advantage of BOD environment is produced bacterial strain, yeast and photosynthetic bacteria that preliminary screening is gone out carry out compound cultivation, again complex culture medium and spore bacteria are carried out compound cultivation, again complex culture medium and milk-acid bacteria are carried out compound cultivation, so repeatedly, screen at last and determine that each bacterial classification adapts to the production bacterial strain of compound culture environment, determine the fermentation manufacturing technique and the culture medium prescription of three-step approach on this basis, it is the first step, earlier selected yeast and photosynthetic bacteria bacterial strain are carried out compound cultivation, substratum is starch 50～200g, glucose 10～30g, peptone 3～8g, potassium dihydrogen sulfate 1～5g, water 1000ml, pH is 4.5～7.0, culture temperature is 20～35 ℃, incubation time is 8～36 hours, second step, above-mentioned complex culture medium and the spore bacteria of selecting are carried out compound cultivation, substratum is starch 30～80g, molasses 5～15g, egg-white powder 5～15g, sodium-chlor 1～5g, water 1000ml, pH is 4.5～7.0, culture temperature is 20～35 ℃, incubation time is 10～48 hours, the 3rd step was carried out compound cultivation with above-mentioned complex culture medium and milk-acid bacteria, culture medium prescription is starch 20～80g, molasses 10～30g, dipotassium hydrogen phosphate 0.5～3g, water 1000ml, pH is 2.8～4.8, culture temperature is 20～35 ℃, incubation time is 12～72 hours, compound cultivation through above-mentioned three steps, just can produce probiotic bacterium composite fungus agent of the present invention, this microbial inoculum can directly apply to the purifying treatment of sewage and the fermentative deodorizing of organic solid rubbish is handled.
The present invention produces the probiotic bacterium composite fungus agent owing to adopted photosynthetic bacteria, yeast, spore bacteria and milk-acid bacteria to carry out compound cultivation, utilized the characteristic that checks and balance, utilizes mutually and have complementary functions between the various microbial bacterias fully, by its application in organic sewage is handled, can rapidly beneficial microorganism bacterium in the sewage environment be developed into dominant microflora, reach the inhibition pernicious bacteria, decomposing organic pollutant matter reduces BOD, COD, the effect that purifies water.
Embodiment:
Embodiment: the manufacture method that is used for the probiotic bacterium composite fungus agent of sewage disposal
The milk-acid bacteria that we adopt is Lactobacterium acidophilum (Lactobacillus acidophilus), yeast is saccharomyces cerevisiae (Saccharomyces cerevisiae), photosynthetic bacteria is Rhodopseudomonas palustris (Rhodopseudom onasPalustris), spore bacteria is bacillus subtilis (Bacillussubtilis), starch is selected corn steep liquor starch for use, at first buy Lactobacterium acidophilum from Guangdong Province Institute of Micro-biology bacterial classification center, saccharomyces cerevisiae, Rhodopseudomonas palustris, concentration and separation goes out bacillus subtilis from the earth of rice field, pond again, these bacterial classifications are implanted COD concentration respectively carry out the adaptability domestication greater than 400 organic sewage environment, filter out the advantage that adapts to high COD environment and produce bacterial strain, saccharomyces cerevisiae and Rhodopseudomonas palustris are carried out compound cultivation, again complex culture medium and bacillus subtilis are carried out compound cultivation, again complex culture medium and Lactobacterium acidophilum are carried out compound cultivation, so repeatedly, screen and determine to be fit to the production bacterial strain of composite environment at last, determine the fermentation manufacturing technique and the culture medium prescription of three-step approach on this basis, it is the first step, carry out the compound cultivation of saccharomyces cerevisiae and Rhodopseudomonas palustris earlier, substratum is corn steep liquor 150g, glucose 20g, peptone 5g, potassium dihydrogen sulfate 3g, water 1000ml, pH is 5.5, culture temperature is 28 ℃, incubation time is 24 hours, second step, above-mentioned complex culture medium and bacillus subtilis are carried out compound cultivation, substratum is corn steep liquor 50g, molasses 10g, egg-white powder 10g, sodium-chlor 3g, water 1000ml, pH is 5.0, culture temperature is 28 ℃, incubation time is 12 hours, the 3rd step was carried out compound cultivation with above-mentioned complex culture medium and Lactobacterium acidophilum, culture medium prescription is corn steep liquor 30g, molasses 10g, dipotassium hydrogen phosphate 1g, water 1000ml, pH are 3.3, culture temperature is 28 ℃, and incubation time is 48 hours.
Claims (3)
1, a kind of manufacture method of probiotic bacterium composite fungus agent, it is characterized in that selecting photosynthetic bacteria, yeast, spore bacteria and milk-acid bacteria are basic bacterial classification, these bacterial classifications are implanted COD concentration respectively carry out the adaptability domestication greater than 400 organic sewage environment, preliminary screening goes out each bacterial classification and adapts to high COD, the advantage of BOD environment is produced bacterial strain, yeast and photosynthetic bacteria that preliminary screening is gone out carry out compound cultivation, again complex culture medium and spore bacteria are carried out compound cultivation, again complex culture medium and milk-acid bacteria are carried out compound cultivation, so repeatedly, screen at last and determine that each bacterial classification adapts to the production bacterial strain of compound culture environment, determine the fermentation manufacturing technique and the culture medium prescription of three-step approach on this basis, it is the first step, earlier selected yeast and photosynthetic bacteria bacterial strain are carried out compound cultivation, substratum is starch 50～200g, glucose 10～30g, peptone 3～8g, potassium dihydrogen sulfate 1～5g, water 1000ml, pH is 4.5～7.0, culture temperature is 20～35 ℃, incubation time is 8～36 hours, second step, above-mentioned complex culture medium and the spore bacteria of selecting are carried out compound cultivation, substratum is starch 30～80g, molasses 5～15g, egg-white powder 5～15g, sodium-chlor 1～5g, water 1000ml, pH is 4.5～7.0, culture temperature is 20～35 ℃, incubation time is 10～48 hours, the 3rd step was carried out compound cultivation with above-mentioned complex culture medium and milk-acid bacteria, culture medium prescription is starch 20～80g, molasses 10～30g, dipotassium hydrogen phosphate 0.5～3g, water 1000ml, pH are 2.8～4.8, culture temperature is 20～35 ℃, incubation time is 12～72 hours, through the compound cultivation of above-mentioned three steps, just can produce probiotic bacterium composite fungus agent of the present invention.
2, the manufacture method of probiotic bacterium composite fungus agent according to claim 1, its feature with the milk-acid bacteria of being adopted be Lactobacterium acidophilum (Lactobacillus acidophilus), yeast is saccharomyces cerevisiae (Saccharomyces cerevisiae), photosynthetic bacteria is Rhodopseudomonas palustris (Rhodopseudom onasPalustris), spore bacteria is bacillus subtilis (Bacillussubtilis)
3, the manufacture method of probiotic bacterium composite fungus agent according to claim 1, its feature is being selected corn steep liquor starch for use with the starch that is adopted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101123462A CN100453479C (en) | 2003-11-26 | 2003-11-26 | Probiotic bacterium compound formulation preparation method |
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|---|---|---|---|
| CNB2003101123462A CN100453479C (en) | 2003-11-26 | 2003-11-26 | Probiotic bacterium compound formulation preparation method |
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| Publication Number | Publication Date |
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| CN1544356A true CN1544356A (en) | 2004-11-10 |
| CN100453479C CN100453479C (en) | 2009-01-21 |
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| CNB2003101123462A Expired - Fee Related CN100453479C (en) | 2003-11-26 | 2003-11-26 | Probiotic bacterium compound formulation preparation method |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100400440C (en) * | 2006-01-24 | 2008-07-09 | 沈阳大学 | Efficient ecological sewage treating promoter and its prepn process |
| CN101884423A (en) * | 2010-02-01 | 2010-11-17 | 杨雄 | Intestinal canal regulating probiotic drink and preparation method thereof |
| CN101356950B (en) * | 2008-09-24 | 2011-04-27 | 东北农业大学 | Preparation method of composite bacteria fermentation bed |
| CN103103148A (en) * | 2012-12-18 | 2013-05-15 | 山东西王糖业有限公司 | Method for screening Bacillus subtilis applicable to taking corn starch sewage as carbon source of culture medium and production method of wettable powder |
| CN101155923B (en) * | 2005-01-03 | 2013-06-05 | 第一研究实验室公司 | Method for producing probiotically derived compounds |
| CN101600795B (en) * | 2006-10-04 | 2013-06-05 | 达能日尔维公司 | Method for obtaining variants of lactic bacteria useful in the production of k2 vitamin and applications for the preparation of food products |
| CN103553739A (en) * | 2013-09-30 | 2014-02-05 | 眉县果业技术推广服务中心 | Production method for liquid organic fertilizer by using starch wastewater |
| CN103937673A (en) * | 2014-04-25 | 2014-07-23 | 浙江农林大学天目学院 | Soil microbial culture medium and preparation method thereof |
| CN104186960A (en) * | 2014-07-02 | 2014-12-10 | 广州大学 | Epinephelus coioides feed additive and application method thereof |
| CN104925943A (en) * | 2015-07-03 | 2015-09-23 | 山东通江三达环保科技有限公司 | Domestic sewage and garbage leachate treatment agent and preparation method therefor |
| CN105713863A (en) * | 2016-03-30 | 2016-06-29 | 林映津 | Production technology of microbial agent through recycling of bamboo shoot processing wastewater |
| CN105941198A (en) * | 2016-04-22 | 2016-09-21 | 苏州依科曼生物农业科技有限公司 | Micro-ecological mixed preparation and application thereof |
| CN105969699A (en) * | 2016-07-12 | 2016-09-28 | 佛山杰致信息科技有限公司 | Agricultural ecological probiotic composite microbial inoculant |
| CN110408572A (en) * | 2019-08-19 | 2019-11-05 | 南宁职业技术学院 | The preparation method and applications of microorganism fungus kind |
| CN111040970A (en) * | 2019-12-27 | 2020-04-21 | 河北九华勘查测绘有限责任公司 | Compound microbial agent, preparation method thereof and application thereof in black and odorous water body remediation |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197842A (en) * | 1997-10-09 | 1998-11-04 | 徐修文 | Microbiological preparation of beneficial microbe colony |
| CN1421402A (en) * | 2001-11-30 | 2003-06-04 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Microbial prepn for treating paper-making effluent and microbe culturing method |
| JP4317964B2 (en) * | 2002-02-06 | 2009-08-19 | 株式会社マサキ・エンヴェック | Wastewater treatment system using humic substances |
| JP2003274938A (en) * | 2002-03-26 | 2003-09-30 | Noriyoshi Moriyama | Useful composition and method for production thereof or the like |
-
2003
- 2003-11-26 CN CNB2003101123462A patent/CN100453479C/en not_active Expired - Fee Related
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101155923B (en) * | 2005-01-03 | 2013-06-05 | 第一研究实验室公司 | Method for producing probiotically derived compounds |
| CN100400440C (en) * | 2006-01-24 | 2008-07-09 | 沈阳大学 | Efficient ecological sewage treating promoter and its prepn process |
| CN101600795B (en) * | 2006-10-04 | 2013-06-05 | 达能日尔维公司 | Method for obtaining variants of lactic bacteria useful in the production of k2 vitamin and applications for the preparation of food products |
| CN101356950B (en) * | 2008-09-24 | 2011-04-27 | 东北农业大学 | Preparation method of composite bacteria fermentation bed |
| CN101884423A (en) * | 2010-02-01 | 2010-11-17 | 杨雄 | Intestinal canal regulating probiotic drink and preparation method thereof |
| CN101884423B (en) * | 2010-02-01 | 2013-04-17 | 杨雄 | Intestinal canal regulating probiotic drink and preparation method thereof |
| CN103103148A (en) * | 2012-12-18 | 2013-05-15 | 山东西王糖业有限公司 | Method for screening Bacillus subtilis applicable to taking corn starch sewage as carbon source of culture medium and production method of wettable powder |
| CN103553739B (en) * | 2013-09-30 | 2015-06-10 | 眉县果业技术推广服务中心 | Production method for liquid organic fertilizer by using starch wastewater |
| CN103553739A (en) * | 2013-09-30 | 2014-02-05 | 眉县果业技术推广服务中心 | Production method for liquid organic fertilizer by using starch wastewater |
| CN103937673A (en) * | 2014-04-25 | 2014-07-23 | 浙江农林大学天目学院 | Soil microbial culture medium and preparation method thereof |
| CN103937673B (en) * | 2014-04-25 | 2016-06-08 | 浙江农林大学天目学院 | A kind of soil microorganism culture medium and preparation method thereof |
| CN104186960A (en) * | 2014-07-02 | 2014-12-10 | 广州大学 | Epinephelus coioides feed additive and application method thereof |
| CN104186960B (en) * | 2014-07-02 | 2017-01-11 | 广州大学 | Epinephelus coioides feed additive and application method thereof |
| CN104925943A (en) * | 2015-07-03 | 2015-09-23 | 山东通江三达环保科技有限公司 | Domestic sewage and garbage leachate treatment agent and preparation method therefor |
| CN105713863A (en) * | 2016-03-30 | 2016-06-29 | 林映津 | Production technology of microbial agent through recycling of bamboo shoot processing wastewater |
| CN105941198A (en) * | 2016-04-22 | 2016-09-21 | 苏州依科曼生物农业科技有限公司 | Micro-ecological mixed preparation and application thereof |
| CN105969699A (en) * | 2016-07-12 | 2016-09-28 | 佛山杰致信息科技有限公司 | Agricultural ecological probiotic composite microbial inoculant |
| CN110408572A (en) * | 2019-08-19 | 2019-11-05 | 南宁职业技术学院 | The preparation method and applications of microorganism fungus kind |
| CN111040970A (en) * | 2019-12-27 | 2020-04-21 | 河北九华勘查测绘有限责任公司 | Compound microbial agent, preparation method thereof and application thereof in black and odorous water body remediation |
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| CN100453479C (en) | 2009-01-21 |
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Application publication date: 20041110 Assignee: Heshan Sewete Biological Product Co.,Ltd. Assignor: Yang Xiong Contract record no.: 2010440000306 Denomination of invention: Probiotic bacterium compound formulation preparation method Granted publication date: 20090121 License type: Exclusive License Record date: 20100330 |
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Effective date of registration: 20200403 Address after: 529725 Jiaotong quarry, Zhongxin village, Taoyuan Town, Heshan City, Jiangmen City, Guangdong Province Co-patentee after: GUANGDONG XINDI BIOLOGICAL ENGINEERING RESEARCH INSTITUTE Co.,Ltd. Patentee after: Heshan Sewete Biological Product Co.,Ltd. Address before: 510640 Guangdong city of Guangzhou province Wushan of Guangdong Academy of Agricultural Sciences West 22-803 Patentee before: Yang Xiong |
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