CN101884423A - Intestinal canal regulating probiotic drink and preparation method thereof - Google Patents

Intestinal canal regulating probiotic drink and preparation method thereof Download PDF

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CN101884423A
CN101884423A CN2010102182275A CN201010218227A CN101884423A CN 101884423 A CN101884423 A CN 101884423A CN 2010102182275 A CN2010102182275 A CN 2010102182275A CN 201010218227 A CN201010218227 A CN 201010218227A CN 101884423 A CN101884423 A CN 101884423A
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culture medium
starch
intestinal canal
cultivation
molasses
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CN101884423B (en
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杨雄
黄洁容
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GUANGDONG XINDI BIOLOGICAL ENGINEERING RESEARCH INSTITUTE Co.,Ltd.
Heshan Sewete Biological Product Co.,Ltd.
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杨雄
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Abstract

The invention discloses an intestinal canal regulating probiotic drink. The drink is prepared by performing the following steps of: (1), inoculating microzyme into a culture medium, and compositely culturing the microzyme at a cultivation temperature of 20 to 35 DEG C for 10 to 48 hours to obtain composite culture solution, wherein the culture medium contains 30 to 80g of starch, 5 to 15g of molasses, 5 to 15g of meringue powder, 1 to 5g of sodium chloride and 1,000ml of water, and the pH value of the solution is 4.5 to 7.0; and (2), inoculating the composite culture solution and lactobacillus into a new culture medium, and compositely culturing the mixture for 12 to 72 hours at the cultivation temperature of 20 to 35 DEG C to obtain the probiotic composite drink, wherein the formula of the new culture medium is that the new culture medium contains 20 to 80g of starch, 10 to 30g of molasses, 0.5 to 3g of dipotassium phosphate and 1,000ml of water and the pH value is 2.8 to 4.8. The invention also discloses a preparation method for the intestinal canal regulating probiotic drink. The drink provided by the invention can supply beneficial bacteria in human bodies to form a dominant bacterium group of the beneficial bacteria in human bodies so that intestinal canal micro-ecosystems of human bodies is regulated and problems of astriction, diarrhea and the like of intestinal canals of human bodies, which are caused by chaos of the micro-ecosystems, are solved.

Description

A kind of intestinal canal regulating probiotic drink and manufacture method thereof
Technical field
The present invention relates to a kind of probiotic beverage, particularly a kind of intestinal canal regulating probiotic drink.The invention still further relates to the manufacture method of this intestinal canal regulating probiotic drink.
Background technology
The earth of living at us exists countless microorganisms, from the air to the seabed, from the South Pole and equator, from human body, animal, ubiquitous in the body of plant to body surface, microorganism is the most ancient on the earth, the primitive organism type that quantity is maximum, be to keep the of paramount importance role of the ecological balance on the earth, there is not the existence of microorganism, just there is not human living space on the earth, the purification of rivers, the regeneration of rubbish even the corruption of corpse, all be the result of microbial action, but progress and industrialized development along with human society, abuse of antibiotics, the hormone phenomenon is very general, the original ecological balance is destroyed gradually, microecosystem with regard to the body inside of making things difficult for others, along with antibiotic, hormone, various chemicalses use in a large number, add that food brings the influence of the various poisonous and harmful substances of human body, microorganism species in the human body is seriously damaged, the various microbial bacterias that counteract each other have lost original balance, by the metabolite that these microbial bacterias produced, enzyme system, immune system all has been subjected to influence in various degree, Here it is, and why so much inferior health appears in the modern, the main cause of so much queer disease occurs.
Beverage is a kind of modal food that people are used to quench one's thirst, the function of beverage is more and more at present, except traditional function of quenching one's thirst, other function also is developed gradually at present, for example nutritious drink, the beverage that lies fallow, fiery beverage etc. falls, but also not occurring with the microorganism in the market is main component, can regulate the beverage of human body intestinal microecology system.
Summary of the invention
First purpose of the present invention provides a kind of intestinal canal regulating probiotic drink, this beverage can replenish beneficial bacteria in the human body, make beneficial bacterium formation dominant microflora in the human body, regulate human body intestinal microecology system thereby reach, solve human body intestinal canal because the problems such as just closing, have loose bowels that the microecosystem confusion causes.
Second purpose of the present invention provides the manufacture method of this probiotic beverage.
First purpose of the present invention realizes by following technical measures: a kind of intestinal canal regulating probiotic drink, and it gets by the following steps preparation:
(1) getting saccharomycete is seeded to and carries out compound cultivation in the culture medium obtained complex culture medium in 10~48 hours under 20~35 ℃ cultivation temperature, described culture medium is starch-containing 30~80g in the 1000ml water, molasses 5~15g, egg-white powder 5~15g, sodium chloride 1~5g, and pH is 4.5~7.0;
(2) complex culture medium in the step (1) and lactobacillus inoculum are carried out compound cultivation to the new culture medium under 20~35 ℃ cultivation temperature and obtained the probio composite beverage in 12~72 hours, described new culture medium prescription is the starch-containing 20~80g of water 1000ml, molasses 10~30g, dipotassium hydrogen phosphate 0.5~3g, pH are 2.8~4.8.
Saccharomycete in the step of the present invention (1) is seeded in the culture medium according to the inoculum concentration of routine, just can realize purpose of the present invention.But as the preferred saccharomycetic inoculum concentration of the present invention in 3 ‰ of culture medium cumulative volume~6 ‰ scopes.
Same, the lactic acid bacteria in the described step (1) also can adopt conventional inoculum concentration to inoculate, and its preferred inoculum concentration is in 0.5%~1.5% scope of culture medium cumulative volume.
The present invention can do following improvement: get saccharomycete in the described step (1) and bacillus is seeded in the culture medium, and carry out compound cultivation obtained complex culture medium in 10~48 hours under 20~35 ℃ cultivation temperature.
The inoculum concentration of described bacillus also can adopt conventional inoculum concentration to inoculate, and its preferred inoculum concentration is in 0.5 ‰ of culture medium cumulative volume~1.5 ‰ scopes.
Starch of the present invention can adopt existing can be used for to be fit to the various starch that saccharomycete, bacillus and lactic acid bacteria are cultivated, and comprises cornstarch, wheaten starch, farina etc.
Second purpose of the present invention is to realize by following technical measures, the manufacture method of above-mentioned intestinal canal regulating probiotic drink, and it may further comprise the steps:
(1) getting saccharomycete is seeded to and carries out compound cultivation in the culture medium obtained complex culture medium in 10~48 hours under 20~35 ℃ cultivation temperature, described culture medium is starch-containing 30~80g in the 1000ml water, molasses 5~15g, egg-white powder 5~15g, sodium chloride 1~5g, and pH is 4.5~7.0;
(2) complex culture medium in the step (1) and lactobacillus inoculum are carried out compound cultivation to the new culture medium under 20~35 ℃ cultivation temperature and obtained the probio composite beverage in 12~72 hours, described new culture medium prescription is the starch-containing 20~80g of water 1000ml, molasses 10~30g, dipotassium hydrogen phosphate 0.5~3g, pH are 2.8~4.8.
The present invention can do following improvement: get saccharomycete in the described step (1) and bacillus is seeded in the culture medium, and carry out compound cultivation obtained complex culture medium in 10~48 hours under 20~35 ℃ cultivation temperature.
The inoculum concentration of described bacillus also can adopt conventional inoculum concentration to inoculate, and its preferred inoculum concentration is in 0.5 ‰ of culture medium cumulative volume~1.5 ‰ scopes.
Saccharomycete in the step of the present invention (1) is seeded in the culture medium according to the inoculum concentration of routine, just can realize purpose of the present invention.But as the preferred saccharomycetic inoculum concentration of the present invention in 3 ‰ of final products gross weight~6 ‰ scopes.
Similarly, the lactic acid bacteria in the described step (1) also can adopt conventional inoculum concentration to inoculate, and its preferred inoculum concentration is in 0.5%~1.5% scope of final products gross weight.
Starch of the present invention can adopt existing can be used for to be fit to the various starch that saccharomycete, bacillus and lactic acid bacteria are cultivated, and comprises cornstarch, wheaten starch, farina etc.
The present invention is owing to adopted saccharomycete, bacillus and lactic acid bacteria carry out compound cultivation and produce the probio composite bacteria agent capable, utilized counteracting each other between the various microbial bacterias fully, check and balance, the characteristic of utilizing mutually and having complementary functions, drink as beverage, the dominant microflora that it is main bacterium that energy forms with these beneficial microbe bacterium in human body intestinal canal rapidly, reach the inhibition pernicious bacteria, microecosystem in the balance enteron aisle, reparation is by the micro-ecological environment of antibiotic destruction, thereby repair destroyed and unbalanced enzyme system and immune system, be particularly suitable for a variety of causes and cause just and close, the disease that intestinal microecology system problem such as have loose bowels causes is used.
Below in conjunction with clinical testing beneficial effect of the present invention is described:
Be used for the treatment of the clinical testing that diabetic complication closes once, intestinal canal regulating probiotic drink
Two groups of test branch control group and test group, test group adopts intestinal canal regulating probiotic drink of the present invention, control group is with No. 1 beverage in the common world, every group of patient is 13, and diabetic history is 5-15, minimum 35 years old of experimenter person's age, maximum 68 years old, 5 women of 8 women of test group, 6 male sex of 7 women of control group all are accompanied with the serious complication of just closing.Experimental program: sooner or later respectively drink 50 milliliters of beverages every day first month, drank 50 milliliters of beverages every one day per morning and evening in second month, respectively drank 50 milliliters of beverages on the 3rd month week about sooner or later.Experimental result: 13 patients' of control group the syndrome of just closing is not improved, 13 patients of experimental group, 11 people just close symptom and begin successively after 3 days to improve from drinking probiotic beverage, have clear improvement after 1 month, but there are 2 people (to be the women, age was respectively 58 years old and 62 years old) 3 of tests are monthly does not improve, and this 2 routine patient is checked to analyze find, and organic variation two has oversizely taken place because of just closing the time in per capita.
Can significantly find out from curative effect, probiotic beverage provided by the invention has the obvious treatment effect to the complication of just closing that diabetes cause, as long as organic variation does not take place, just to close symptom and all can obviously improve, its reason is: the diabetes patient uses antibiotic for a long time, microecosystem in the body has been caused serious damage, caused just closing complication, after use this product, owing to repaired microecosystem in the body, make that microecosystem restores balance in the body, thereby make and just close symptom and be improved.
Two, intestinal canal regulating probiotic drink is used for the treatment of the clinical testing that stool is not smooth or have loose bowels
During year December in January, 2008 to 2009, utilize the employee of the intestinal canal regulating probiotic drink of the present invention biological products Co., Ltd new to carry out irregular clinical testing in Heshan City, according to experimental record, there are 122 person-times in the period of two because just close or the problem of having loose bowels has been drunk this probiotic beverage, wherein drink have 82 person-times of product of the present invention because of the just problem of closing, wherein the male sex is 52 person-times, 30 person-times of women, and all obtained tangible improvement.The cause problem of having loose bowels is drunk have 40 person-times of product of the present invention, and wherein the male sex is 20 person-times, 20 person-times of women, and all obtained tangible improvement.
From above result as seen, intestinal canal regulating probiotic drink provided by the invention has the effect of two-way intestinal canal regulating.
The specific embodiment
Embodiment one:
Lactic acid bacteria is adopted lactobacillus acidophilus (Lactobacillus acidophilus) in the present embodiment, and saccharomycete adopts saccharomyces cerevisiae (Saccharomyces cerevisiae), and starch adopts cornstarch.
(1) getting saccharomyces cerevisiae is seeded to by 5 ‰ of culture medium cumulative volume and carries out compound cultivation in the culture medium obtained complex culture medium in 12 hours under 28 ℃ cultivation temperature, wherein, culture medium is to contain cornstarch 50g, molasses 10g, egg-white powder 10g, sodium chloride 3g in the 1000ml water, and pH is 5.0.
(2) get complex culture medium in the step (1) and lactobacillus acidophilus and be seeded to and carry out compound cultivation in the new culture medium obtained the probio composite beverage in 48 hours under 28 ℃ cultivation temperature, the inoculum concentration of lactobacillus acidophilus is 1% of a culture medium cumulative volume.New culture medium prescription contains corn steep liquor starch 30g for water 1000ml, molasses 10g, and dipotassium hydrogen phosphate 1g, pH are 3.3.
Embodiment two:
Lactic acid bacteria is adopted lactobacillus acidophilus (Lactobacillus acidophilus) in the present embodiment, and saccharomycete adopts saccharomyces cerevisiae (Saccharomyces cerevisiae), and starch adopts corn steep liquor starch.
(1) getting saccharomyces cerevisiae is seeded to by 3 ‰ of culture medium cumulative volume and carries out compound cultivation in the culture medium obtained complex culture medium in 10 hours under 35 ℃ cultivation temperature, wherein, culture medium is to contain cornstarch 30g, molasses 5g, egg-white powder 5g, sodium chloride 1g in the 1000ml water, and pH is 5.0.
(2) get complex culture medium in the step (1) and lactobacillus acidophilus and be seeded to and carry out compound cultivation in the new culture medium obtained the probio composite beverage in 50 hours under 35 ℃ cultivation temperature, the inoculum concentration of lactobacillus acidophilus is 1.5% of a culture medium cumulative volume.New culture medium prescription contains corn steep liquor starch 80g for water 1000ml, molasses 30g, and dipotassium hydrogen phosphate 3g, pH are 4.8.
Embodiment three:
Lactic acid bacteria is adopted lactobacillus acidophilus (Lactobacillus acidophilus) in the present embodiment, saccharomycete adopts saccharomyces cerevisiae (Saccharomyces cerevisiae), bacillus is adopted bacillus licheniformis (Bacillus licheniformis), and starch adopts corn steep liquor starch.
(1) getting saccharomyces cerevisiae is seeded in the culture medium by 5 ‰ of culture medium cumulative volume, getting bacillus licheniformis is seeded in the culture medium by 1 ‰ of culture medium cumulative volume, under 28 ℃ cultivation temperature, carry out compound cultivation and obtained complex culture medium in 12 hours, wherein, culture medium is to contain cornstarch 50g, molasses 10g, egg-white powder 10g, sodium chloride 3g in the 1000ml water, and pH is 5.0.
(2) get complex culture medium in the step (1) and lactobacillus acidophilus and be seeded to and carry out compound cultivation in the new culture medium obtained the probio composite beverage in 48 hours under 28 ℃ cultivation temperature, the inoculum concentration of lactobacillus acidophilus is 1% of a culture medium cumulative volume.New culture medium prescription contains corn steep liquor starch 30g for water 1000ml, molasses 10g, and dipotassium hydrogen phosphate 1g, pH are 3.3.
Embodiment four:
Lactic acid bacteria is adopted lactobacillus acidophilus (Lactobacillus acidophilus) in the present embodiment, saccharomycete adopts saccharomyces cerevisiae (Saccharomyces cerevisiae), bacillus is adopted bacillus licheniformis (Bacillus licheniformis), and starch adopts corn steep liquor starch.
(1) getting saccharomyces cerevisiae is seeded in the culture medium by 4.5 ‰ of culture medium cumulative volume, getting bacillus licheniformis is seeded in the culture medium by 1.5 ‰ of culture medium cumulative volume, under 20 ℃ cultivation temperature, carry out compound cultivation and obtained complex culture medium in 48 hours, wherein, culture medium is to contain cornstarch 60g, molasses 14g, egg-white powder 14g, sodium chloride 4.5g in the 1000ml water, and pH is 6.0.
(2) get complex culture medium in the step (1) and lactobacillus acidophilus and be seeded to and carry out compound cultivation in the new culture medium obtained the probio composite beverage in 72 hours under 25 ℃ cultivation temperature, the inoculum concentration of lactobacillus acidophilus is 0.9% of a culture medium cumulative volume.New culture medium prescription contains corn steep liquor starch 20g for water 1000ml, molasses 10g, and dipotassium hydrogen phosphate 1g, pH are 2.8.
Embodiment five:
Lactic acid bacteria is adopted lactobacillus acidophilus (Lactobacillus acidophilus) in the present embodiment, saccharomycete adopts saccharomyces cerevisiae (Saccharomyces cerevisiae), bacillus is adopted bacillus licheniformis (Bacillus licheniformis), and starch adopts corn steep liquor starch.
(1) getting saccharomyces cerevisiae is seeded in the culture medium by 6 ‰ of culture medium cumulative volume, getting bacillus licheniformis is seeded in the culture medium by 0.5 ‰ of culture medium cumulative volume, under 3028 ℃ cultivation temperature, carry out compound cultivation and obtained complex culture medium in 24 hours, wherein, culture medium is to contain cornstarch 80g, molasses 15g, egg-white powder 15g, sodium chloride 5g in the 1000ml water, and pH is 7.0.
(2) get complex culture medium in the step (1) and lactobacillus acidophilus and be seeded to and carry out compound cultivation in the new culture medium obtained the probio composite beverage in 60 hours under 30 ℃ cultivation temperature, the inoculum concentration of lactobacillus acidophilus is 0.5% of a culture medium cumulative volume.New culture medium prescription contains corn steep liquor starch 60g for water 1000ml, molasses 25g, and dipotassium hydrogen phosphate 2g, pH are 4.0.
The above embodiment of the present invention only is used for the present invention is set forth, yet protection scope of the present invention is not only to be confined to above embodiment.The person of an ordinary skill in the technical field only need get saccharomycete, bacillus and lactic acid bacteria as raw material, according to content disclosed by the invention, all can realize purpose of the present invention.

Claims (7)

1. an intestinal canal regulating probiotic drink is characterized in that, it gets by the following steps preparation:
(1) getting saccharomycete is seeded to and carries out compound cultivation in the culture medium obtained complex culture medium in 10~48 hours under 20~35 ℃ cultivation temperature, described culture medium is starch-containing 30~80g in the 1000ml water, molasses 5~15g, egg-white powder 5~15g, sodium chloride 1~5g, and pH is 4.5~7.0;
(2) complex culture medium in the step (1) and lactobacillus inoculum are carried out compound cultivation to the new culture medium under 20~35 ℃ cultivation temperature and obtained the probio composite beverage in 12~72 hours, described new culture medium prescription is the starch-containing 20~80g of water 1000ml, molasses 10~30g, dipotassium hydrogen phosphate 0.5~3g, pH are 2.8~4.8.
2. intestinal canal regulating probiotic drink according to claim 1 is characterized in that, the saccharomycetic inoculum concentration in the described step (1) is in 3 ‰ of culture medium cumulative volume~6 ‰ scopes.
3. intestinal canal regulating probiotic drink according to claim 2 is characterized in that, the inoculum concentration of the lactic acid bacteria in the described step (1) is in 0.5%~1.5% scope of culture medium cumulative volume.
4. according to claim 1 or 2 or 3 described intestinal canal regulating probiotic drinks, it is characterized in that, get saccharomycete in the described step (1) and bacillus is seeded in the culture medium, and under 20~35 ℃ cultivation temperature, carry out compound cultivation and obtained complex culture medium in 10~48 hours.
5. intestinal canal regulating probiotic drink according to claim 4 is characterized in that, the inoculum concentration of described bacillus is in 0.5 ‰ of culture medium cumulative volume~1.5 ‰ scopes.
6. the manufacture method of the described intestinal canal regulating probiotic drink of the arbitrary claim of claim 1~3 is characterized in that it may further comprise the steps:
(1) gets saccharomycete in the described step (1) and bacillus is seeded in the culture medium, and under 20~35 ℃ cultivation temperature, carry out compound cultivation and obtained complex culture medium in 10~48 hours, described culture medium is starch-containing 30~80g in the 1000ml water, molasses 5~15g, egg-white powder 5~15g, sodium chloride 1~5g, and pH is 4.5~7.0;
(2) complex culture medium in the step (1) and lactobacillus inoculum are carried out compound cultivation to the new culture medium under 20~35 ℃ cultivation temperature and obtained the probio composite beverage in 12~72 hours, described new culture medium prescription is the starch-containing 20~80g of water 1000ml, molasses 10~30g, dipotassium hydrogen phosphate 0.5~3g, pH are 2.8~4.8.
7. the manufacture method of the described intestinal canal regulating probiotic drink of the arbitrary claim of claim 4~5 is characterized in that it may further comprise the steps:
(1) getting saccharomycete is seeded to and carries out compound cultivation in the culture medium obtained complex culture medium in 10~48 hours under 20~35 ℃ cultivation temperature, described culture medium is starch-containing 30~80g in the 1000ml water, molasses 5~15g, egg-white powder 5~15g, sodium chloride 1~5g, and pH is 4.5~7.0;
(2) complex culture medium in the step (1) and lactobacillus inoculum are carried out compound cultivation to the new culture medium under 20~35 ℃ cultivation temperature and obtained the probio composite beverage in 12~72 hours, described new culture medium prescription is the starch-containing 20~80g of water 1000ml, molasses 10~30g, dipotassium hydrogen phosphate 0.5~3g, pH are 2.8~4.8.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102334712A (en) * 2011-08-25 2012-02-01 罗四维 Preparation method of solution for improving enteric microorganisms
CN103937673A (en) * 2014-04-25 2014-07-23 浙江农林大学天目学院 Soil microbial culture medium and preparation method thereof
CN106261423A (en) * 2016-09-30 2017-01-04 皖西学院 A kind of Traditional Chinese medicine probiotic composite health care beverage and preparation method thereof
CN113430150A (en) * 2021-08-11 2021-09-24 真爱生命生物技术(重庆)有限公司 Probiotics and application thereof

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102334712A (en) * 2011-08-25 2012-02-01 罗四维 Preparation method of solution for improving enteric microorganisms
CN102334712B (en) * 2011-08-25 2015-07-01 罗四维 Preparation method of solution for improving enteric microorganisms
CN103937673A (en) * 2014-04-25 2014-07-23 浙江农林大学天目学院 Soil microbial culture medium and preparation method thereof
CN103937673B (en) * 2014-04-25 2016-06-08 浙江农林大学天目学院 A kind of soil microorganism culture medium and preparation method thereof
CN106261423A (en) * 2016-09-30 2017-01-04 皖西学院 A kind of Traditional Chinese medicine probiotic composite health care beverage and preparation method thereof
CN113430150A (en) * 2021-08-11 2021-09-24 真爱生命生物技术(重庆)有限公司 Probiotics and application thereof

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