CN110408572A - The preparation method and applications of microorganism fungus kind - Google Patents
The preparation method and applications of microorganism fungus kind Download PDFInfo
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Abstract
Present invention discloses a kind of microorganism fungus kind preparation method and applications, preparation method has following steps: 1) first being respectively connected to cultivate in culture medium by lactobacillus, lactic acid bacteria, saccharomycete, bacillus and photosynthetic bacteria, obtain first class inoculum；2) first class inoculum of bacillus and photosynthetic bacteria is respectively connected to mixing in the first class inoculum access culture medium for expanding numerous culture and other bacterium in culture medium again and expands numerous culture, obtain second class inoculum；3) it finally is respectively connected to the second class inoculum that bacillus, photosynthetic bacteria and other bacterium are mixed to expand numerous culture in culture medium, obtains three-class strain.Microorganism fungus kind made from above-mentioned preparation method is used to again to handle organic sewage.Preparation method of the invention is easy to operate, and the survival rate for turning out the microbial bacteria come is high, is used for the strain prepared to handle organic wastewater, can reduce or removes water body stench, reduces waste water COD, realizes sewage treatment purpose up to standard.
Description
Technical field
The present invention relates to a kind of preparation method and applications of microorganism fungus kind.
Background technique
It is organic containing carbohydrate, protein, grease etc. in the waste water such as food processing, livestock and poultry cultivation, sanitary sewage
Substance.These substances can be decomposed with suspending or dissolved state is present in sewage by the biological chemistry action of microorganism.This
Kind pollutant can cause oxygen in water to reduce, and influence the growth of fish and other aquatiles.After oxygen in water has consumed, have
Machine object carries out anaerobic fermentation, generates the bad smells such as hydrogen sulfide, ammonia and mercaptan, causes water pollution, serious to pollute environment and people
Living environment.Oxidation pond technology is used at this stage, handles waste water by the probiotic microorganisms in the pool, but in natural conditions
Under due in polluted-water spoilage organisms be often dominant microflora, probiotic microorganisms bacterium is difficult to play a role, and also suffers from advantage
Spoilage organisms inhibit growth, so using this method processing sewage typical time very long and during this usually with rotten
The growth for losing bacterium can give out a foul smell gas.
Summary of the invention
It is an object of the invention in view of the above-mentioned drawbacks in the prior art, provide a kind of preparation method of microorganism fungus kind
And its application, it is applied in organic pollution aquifer by the microorganism fungus kind that will be prepared, so that microbial bacteria obtained becomes excellent
Gesture flora and the growth for inhibiting spoilage organisms, thus pollution administration water body stench and purification sewage.
For achieving the above object, present invention employs following technical solutions:
Present invention firstly provides a kind of preparation methods of microorganism fungus kind, comprising the following steps:
1) first lactobacillus, lactic acid bacteria, saccharomycete, bacillus and photosynthetic bacteria are respectively connected to cultivate in culture medium, are obtained
To first class inoculum；
2) first class inoculum of bacillus and photosynthetic bacteria is respectively connected to again expand numerous culture in culture medium, and newborn bar
Bacterium, lactic acid bacteria, saccharomycete first class inoculum numerous culture is expanded with mixing in the weight ratio of 1:1:1 access culture medium, obtain second level bacterium
Kind；
3) finally the second class inoculum that bacillus, photosynthetic bacteria and other bacterium are mixed is respectively connected to cultivate
Expand numerous culture in base, obtains three-class strain；Microorganism fungus kind includes above-mentioned first class inoculum, second class inoculum and three-class strain.
In above-mentioned preparation method:
The culture medium of the strains at different levels of bacillus, includes the component matched as follows: peptone 10g/L, powdered beef 3g/L,
Sodium chloride 5g/L.The culture medium of the strains at different levels of photosynthetic bacteria includes the component matched as follows: glucose 10g/L, biphosphate
Potassium 0.2g/L, bitter salt 0.2g/L, sodium chloride 0.2g/L, calcium sulphate dihydrate 0.1g/L, calcium carbonate 5g/L.Other bacterium
Strains at different levels culture medium, include the component matched as follows: peptone 10g/L, beef extract 10g/L, yeast extract 5g/L, lemon
Sour 2g/L, glucose 20g/L, Tween 80 1ml/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.58g/L, sulfuric acid
Manganese 0.25g/L.
Spawn incubation condition in three steps is 32 DEG C~38 DEG C of temperature, pH value 3.5~7.0.
Bacillus and photosynthetic bacteria in three steps will constantly convey oxygen in preceding 15 minutes domestic demands that culture starts,
Later at interval of 8 hours supplements, 15 minutes oxygen, until culture is completed.
It is second class inoculum that first class inoculum, which expands numerous culture by 1:100,；It is three-class strain that second class inoculum, which expands numerous culture by 1:10,.
The present invention also provides a kind of applications by microorganism fungus kind made from above-mentioned preparation method in processing organic sewage.
Further, which is pig raising blowdown sewage, processing method are as follows: by lactobacillus, lactic acid bacteria, saccharomycete
First class inoculum and foodstuff press the weight ratio mixed feeding live pig of 1:100；The second class inoculum of lactobacillus, lactic acid bacteria, saccharomycete is thrown
Put pig house；Three-class strain is launched with sewage into cesspool.
Wherein, the put-on method of three-class strain are as follows: when the sewage condition of sewage draining exit is 8000mg/L >=COD > 6000mg/
L, when pH=5.5~6.5, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:3, the strain amount launched daily and
The weight ratio of sewage drainage amount is 1:2500~3000；When the sewage condition in cesspool is 6000mg/L >=COD > 3000mg/
L, when pH=5.8~6.2, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:3, the strain amount launched daily and
The weight ratio of sewage drainage amount is 1:20000~30000；When the sewage condition in cesspool is 3000mg/L >=COD >
When 1500mg/L, pH=6.8~7.2, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:2, are launched daily
The weight ratio of strain amount and sewage drainage amount is 1:20000~30000；When the sewage condition in cesspool be 1500mg/L >=
When COD > 800mg/L, pH=7.3~7.6, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:1, are thrown daily
The weight ratio of the strain amount and sewage drainage amount put is 1:20000~30000；When the sewage condition in cesspool is 800mg/L
When >=COD >=100mg/L, pH > 7.5, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 3:1, are launched daily
The weight ratio of strain amount and sewage drainage amount is 1:20000~30000.
Further, organic when organic sewage is other organic sewages, and its COD concentration is 20000~100mg/L
The processing method of sewage is to launch three-class strain into cesspool with sewage.
Wherein, the put-on method of three-class strain are as follows: when the sewage condition of sewage draining exit is 20000mg/L >=COD > 6000mg/
L, when pH=3.0~5.0, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:4, the strain amount launched daily and
The weight ratio of sewage drainage amount is 1:2500~3000；When the sewage condition in cesspool is 6000mg/L >=COD > 3000mg/
L, when pH=3.5~7.0, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:4, the strain amount launched daily and
The weight ratio of sewage drainage amount is 1:20000~30000；When the sewage condition in cesspool is 3000mg/L >=COD >
When 1500mg/L, pH=4.0~5.0, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:3, are launched daily
The weight ratio of strain amount and sewage drainage amount is 1:20000~30000；When the sewage condition in cesspool be 1500mg/L >=
When COD > 800mg/L, pH=5.0~6.0, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 1:1, are thrown daily
The weight ratio of the strain amount and sewage drainage amount put is 1:20000~30000；When the sewage condition in cesspool is 800mg/L
When >=COD >=100mg/L, pH=5.0~6.5, photosynthetic bacteria strain and other strains are launched according to the weight ratio of 3:1, daily
The strain amount of dispensing and the weight ratio of sewage drainage amount are 1:20000~30000.
Compared with the prior art, present invention has an advantage that
Microorganism fungus kind preparation method is easy to operate, and the survival rate of probiotic microorganisms bacterium is high, by the microorganism fungus kind of culture
For organic sewage processing, to become dominant microflora and inhibit the growth of spoilage organisms, to administer polluted-water stench
And sewage has been purified, realize the purpose of the processing up to standard of sewage.
Specific embodiment
To better understand the objects, features and advantages of the present invention, With reference to embodiment
Further unrestricted detailed description is made to technical solution of the present invention.
Embodiment 1
The preparation method of microorganism fungus kind, comprising the following steps:
1) under conditions of cultivation temperature is 32 DEG C~38 DEG C, pH value is 3.5~7.0, by lactobacillus, lactic acid bacteria, yeast
Bacterium, bacillus and photosynthetic bacteria are respectively connected to cultivate in culture medium, when culture medium pH value in the range of 2.5~4.0 and
When color is orange-yellow, first class inoculum is obtained.
2) at temperature identical with step 1) and pH value, the first class inoculum of bacillus and photosynthetic bacteria is respectively connected to
Expand numerous culture by 1:100 in culture medium and obtains second class inoculum in 2~3 days；By the first class inoculum of lactobacillus, lactic acid bacteria, saccharomycete with
1:1:1 weight ratio access culture medium in mix after, then by 1:100 expand it is numerous culture to pH be 3.0 when stop culture, obtain second level
Strain.
3) at temperature identical with step 1) and pH value, finally by bacillus second class inoculum, photosynthetic bacteria second level bacterium
The second class inoculum that kind and other bacterium are mixed is respectively connected in culture medium expand numerous culture 2~3 days by 1:10, obtains three
Grade strain.
In above-mentioned incubation step, the bacillus and photosynthetic bacteria in three steps are in first 15 minutes that culture starts
It needs constantly to convey oxygen, later at interval of supplemental oxygen 15 minutes 8 hours, until culture is completed.The bacterium at different levels of bacillus
The culture medium of kind, includes the component matched as follows: peptone 10g/L, powdered beef 3g/L, sodium chloride 5g/L.Photosynthetic bacteria it is each
The culture medium of grade strain, includes the component matched as follows: glucose 10g/L, potassium dihydrogen phosphate 0.2g/L, bitter salt
0.2g/L, sodium chloride 0.2g/L, calcium sulphate dihydrate 0.1g/L, calcium carbonate 5g/L.The culture medium of the strains at different levels of other bacterium, packet
Containing the component matched as follows: peptone 10g/L, beef extract 10g/L, yeast extract 5g/L, citric acid 2g/L, glucose 20g/L are spat
Temperature 80 1ml/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L.
Embodiment 2
Microorganism three-class strain made from the preparation method of embodiment 1 is used for starch processing organic sewage, concrete operations
Are as follows:
When starch processing organic sewage just discharges when 20000mg/L >=COD > 6000mg/L, pH=3.0~5.0, arranging
Strain is launched by the weight ratio of 1:4 by photosynthetic bacteria strain and other strains at dirty mouth, launches strain amount and sewage drainage daily
The weight ratio of amount is 1:2500~3000.When sewage 6000mg/L >=COD > 3000mg/L, pH=3.0~4.0 in cesspool
When, photosynthetic bacteria strain and other strains are launched by the weight ratio of 1:4, and the strain amount and total Water weight ratio launched daily are 1:
20000~30000.As sewage 3000mg/L >=COD > 1500mg/L, pH=4.0~5.0 in cesspool, photosynthetic bacteria bacterium
Kind and other strains are launched by the weight ratio of 1:3, and the strain amount and total Water weight ratio launched daily are 1:20000~30000.
As sewage 1500mg/L >=COD > 800mg/L, pH=5.0~6.0 in cesspool, photosynthetic bacteria strain and other strains are pressed
The ratio of 1:1 is launched, and launches strain amount daily and total Water weight ratio is 1:20000~30000.When sewage in cesspool
When 800mg/L >=COD >=100mg/L, pH=5.0~6.5, photosynthetic bacteria strain and other strains are launched in the ratio of 3:1, often
It launches strain amount and total Water weight ratio is 1:20000~30000.When in cesspool COD of sewage drop to 100mg/L hereinafter,
Strain can not be launched when pH=6.5~7.5, algae starts to breed with protozoan at this time, becomes advantage in Sewage Environment
Flora, the BOD of final sewage5It can be reduced to 30mg/L, to realize the processing up to standard of sewage.
Embodiment 3
Microorganism first class inoculum, second class inoculum and three-class strain made from the preparation method of embodiment 1 are used for the row of pig raising
Sewage, processing are divided into three parts:
One, the weight ratio mixed feeding that lactobacillus, lactic acid bacteria, the first class inoculum of saccharomycete and foodstuff are pressed to 1:100, can change
Kind mouthfeel and promotion digestion and absorption can make lactobacillus, lactic acid bacteria, saccharomycete to reduce content of organics in excreta
Further it is proliferated.
Two, the second class inoculum of lactobacillus, lactic acid bacteria, saccharomycete is launched into pig house after cleaning pig house every time, makes lactobacillus, cream
Sour bacterium, saccharomycete form dominant microflora and the growth of pathogenic bacteria are inhibited to promote pig healthy growth.
Three, three-class strain is launched into cesspool:
Pig raising blowdown sewage is when just discharging when 8000mg/L >=COD > 6000mg/L, pH=5.5~6.5, sewage draining exit with
It is launched by photosynthetic bacteria strain and other strains by the weight ratio of 1:3 when sewage discharge, launches strain amount and displacement daily
Weight ratio is 1:2500~3000.In cesspool when sewage 6000mg/L >=COD > 3000mg/L, pH=5.8~6.2, light
Bacteria culture and other strains is closed to launch by the ratio of 1:3, launch daily the weight ratio of strain amount and total Water for 1:20000~
30000.As sewage 3000mg/L >=COD > 1500mg/L, pH=6.8~7.2 in cesspool, photosynthetic bacteria strain and other
Strain is launched in the ratio of 1:2, launches strain amount daily and total Water weight ratio is 1:20000~30000.When dirty in cesspool
When water 1500mg/L >=COD > 800mg/L, pH=7.3~7.4, photosynthetic bacteria strain and other strains are thrown in the ratio of 1:1
It puts, the weight ratio for launching strain amount and total Water daily is 1:20000~30000.As sewage 800mg/L >=COD in cesspool
When >=100mg/L, pH > 7.5, photosynthetic bacteria strain and other strains are launched in the ratio of 3:1, launch strain amount and total daily
The weight ratio of water is 1:20000~30000.It can when COD of sewage drops to 100mg/L hereinafter, pH is 7.5 or more in cesspool
Not launch strain, algae starts to be proliferated with protozoan, becomes dominant microflora in Sewage Environment.The BOD of final sewage5It can
It is reduced to 30mg/L, to realize the processing up to standard of sewage.
Claims (10)
1. the preparation method of microorganism fungus kind, which comprises the following steps:
1) first lactobacillus, lactic acid bacteria, saccharomycete, bacillus and photosynthetic bacteria are respectively connected to cultivate in culture medium, obtain one
Grade strain；
2) first class inoculum of bacillus and photosynthetic bacteria is respectively connected to again expand numerous culture and lactobacillus, cream in culture medium
Sour bacterium, saccharomycete first class inoculum numerous culture is expanded with mixing in the weight ratio of 1:1:1 access culture medium, obtain second class inoculum；
3) finally the second class inoculum that bacillus, photosynthetic bacteria and other bacterium are mixed is respectively connected in culture medium
Expand numerous culture, obtains three-class strain；The microorganism fungus kind includes above-mentioned first class inoculum, second class inoculum and three-class strain.
2. preparation method according to claim 1, it is characterised in that:
The culture medium of the strains at different levels of bacillus includes the component matched as follows: peptone 10g/L, powdered beef 3g/L, chlorination
Sodium 5g/L；
The culture medium of the strains at different levels of photosynthetic bacteria includes the component matched as follows: glucose 10g/L, potassium dihydrogen phosphate 0.2g/
L, bitter salt 0.2g/L, sodium chloride 0.2g/L, calcium sulphate dihydrate 0.1g/L, calcium carbonate 5g/L；
The culture medium of the strains at different levels of other bacterium includes the component matched as follows: peptone 10g/L, beef extract 10g/L, yeast
Cream 5g/L, citric acid 2g/L, glucose 20g/L, Tween 80 1ml/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate
0.58g/L, manganese sulfate 0.25g/L.
3. preparation method according to claim 1, it is characterised in that: the Spawn incubation condition in three steps is temperature
32 DEG C~38 DEG C, pH value 3.5~7.0.
4. preparation method according to claim 1, it is characterised in that: bacillus and photosynthetic bacteria in three steps exist
Preceding 15 minutes domestic demands that culture starts will constantly convey oxygen, later at interval of 8 hours supplements, 15 minutes oxygen, until having cultivated
At.
5. preparation method according to claim 1, it is characterised in that: it is second level bacterium that first class inoculum, which expands numerous culture by 1:100,
Kind；It is three-class strain that second class inoculum, which expands numerous culture by 1:10,.
6. application of the microorganism fungus kind made from preparation method described in any one of Claims 1 to 5 in processing organic sewage.
7. application according to claim 6, it is characterised in that: the organic sewage is pig raising blowdown sewage.
8. application according to claim 7, which is characterized in that the processing method of the pig raising blowdown sewage are as follows: by newborn bar
Bacterium, lactic acid bacteria, the first class inoculum of saccharomycete and foodstuff press the weight ratio mixed feeding live pig of 1:100；By lactobacillus, lactic acid bacteria,
The second class inoculum of saccharomycete launches pig house；Three-class strain is launched with sewage into cesspool；
The put-on method of three-class strain are as follows:
When the sewage condition of sewage draining exit be 8000mg/L >=COD > 6000mg/L, pH=5.5~6.5 when, photosynthetic bacteria strain and
Other strains are launched according to the weight ratio of 1:3, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:2500~
3000；
When the sewage condition in cesspool is 6000mg/L >=COD > 3000mg/L, pH=5.8~6.2, photosynthetic bacteria strain
Launched with other strains according to the weight ratio of 1:3, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:20000~
30000；
When the sewage condition in cesspool is 3000mg/L >=COD > 1500mg/L, pH=6.8~7.2, photosynthetic bacteria strain
Launched with other strains according to the weight ratio of 1:2, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:20000~
30000；
When the sewage condition in cesspool is 1500mg/L >=COD > 800mg/L, pH=7.3~7.6, photosynthetic bacteria strain
Launched with other strains according to the weight ratio of 1:1, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:20000~
30000；
When the sewage condition in cesspool is 800mg/L >=COD >=100mg/L, pH > 7.5, photosynthetic bacteria strain and other
Strain is launched according to the weight ratio of 3:1, and the weight ratio of the strain amount and sewage drainage amount launched daily is 1:20000~30000.
9. application according to claim 6, which is characterized in that the COD concentration of the organic sewage is 20000~100mg/
L。
10. application according to claim 9, which is characterized in that the processing method of the organic sewage is by three-class strain
It launches with sewage into cesspool；
The put-on method of three-class strain are as follows:
When the sewage condition of sewage draining exit is 20000mg/L >=COD > 6000mg/L, pH=3.0~5.0, photosynthetic bacteria strain
Launched with other strains according to the weight ratio of 1:4, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:2500~
3000；
When the sewage condition in cesspool is 6000mg/L >=COD > 3000mg/L, pH=3.0~4.0, photosynthetic bacteria strain
Launched with other strains according to the weight ratio of 1:4, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:20000~
30000；
When the sewage condition in cesspool is 3000mg/L >=COD > 1500mg/L, pH=4.0~5.0, photosynthetic bacteria strain
Launched with other strains according to the weight ratio of 1:3, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:20000~
30000；
When the sewage condition in cesspool is 1500mg/L >=COD > 800mg/L, pH=5.0~6.0, photosynthetic bacteria strain
Launched with other strains according to the weight ratio of 1:1, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:20000~
30000；
When the sewage condition in cesspool be 800mg/L >=COD >=100mg/L, pH=5.0~6.5 when, photosynthetic bacteria strain and
Other strains are launched according to the weight ratio of 3:1, the weight ratio of the strain amount and sewage drainage amount launched daily be 1:20000~
30000。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111019862A (en) * | 2019-12-25 | 2020-04-17 | 深圳市东荣生物科技有限责任公司 | Symbiotic bacteria culture medium and symbiotic bacteria culture method |
| CN114128733A (en) * | 2021-11-18 | 2022-03-04 | 南宁职业技术学院 | Preparation method of camellia oil cake |
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