CN1488380A - Use of chinese medicine composition in preparing medicine for anti SARS virus - Google Patents
Use of chinese medicine composition in preparing medicine for anti SARS virus Download PDFInfo
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- CN1488380A CN1488380A CNA031323871A CN03132387A CN1488380A CN 1488380 A CN1488380 A CN 1488380A CN A031323871 A CNA031323871 A CN A031323871A CN 03132387 A CN03132387 A CN 03132387A CN 1488380 A CN1488380 A CN 1488380A
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Abstract
The invention is an application of a Chinese medicine combination to prepare anti-SARS virus drug. The Chinese medicine combination is made of antelope horn, Fritillaria ussuriensis, rhubarb, BaikalSkullcap Root, Lapis Chloriti K, raw plaster, artifical bezoar, liquorice, and so on.
Description
Technical field
The present invention relates to a kind of purposes of Chinese medicine composition, particularly the purposes in the medicine of preparation anti-SARS virus.
Background technology
The applicant has applied for that on August 20th, 2002 name is called the patent of " a kind of Chinese medicine composition and preparation method thereof ", and application number is 02129192.6.In this application, the applicant discloses a kind of Chinese medicine composition, and this Chinese medicine composition is made by following crude drug:
Cornu Saigae Tataricae 8-12 weight portion Bulbus Fritillariae Ussuriensis 12-20 weight portion
Radix Et Rhizoma Rhei 20-35 weight portion Radix Scutellariae 10-18 weight portion
Lapis Chloriti 10-18 weight portion Gypsum Fibrosum 15-25 weight portion
Its preferred compositions prescription is also disclosed in artificial Calculus Bovis 8-12 weight portion Radix Glycyrrhizae 20-35 weight portion this application; the assay method of preparation method and oral liquid etc.; and this Chinese medicine composition purposes, disclosed purposes is treatment flu and anxious, slow property tracheitis.
Summary of the invention
Technical problem to be solved by this invention provides the new application of a kind of Chinese medicine composition in the medicine of preparation anti-SARS virus.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is the application of following Chinese medicine composition in the medicine of preparation anti-SARS virus,
Cornu Saigae Tataricae 8-12 weight portion Bulbus Fritillariae Ussuriensis 12-20 weight portion
Radix Et Rhizoma Rhei 20-35 weight portion Radix Scutellariae 10-18 weight portion
Lapis Chloriti 10-18 weight portion Gypsum Fibrosum 15-25 weight portion
Artificial Calculus Bovis 8-12 weight portion Radix Glycyrrhizae 20-35 weight portion wherein, preferred Chinese medicine composition is,
Cornu Saigae Tataricae 9.45 weight portion Bulbus Fritillariae Ussuriensiss 17.25 weight portions
Radix Et Rhizoma Rhei 31.50 weight portion Radix Scutellariaes 15.75 weight portions
Lapis Chloriti 15.75 weight portion Gypsum Fibrosum 23.62 weight portions
Artificial Calculus Bovis 9.45 weight portion Radix Glycyrrhizaes 31.50 weight portions
Technical problem to be solved by this invention can also further realize by following technical scheme.The application of above-described Chinese medicine composition in the medicine of preparation anti-SARS virus is characterized in that described medicine is said any dosage form on the pharmaceutics.Wherein, preferred dosage form oral liquid.
A kind of preparation method of the medicine of above-mentioned anti-SARS virus is: above eight flavors, Cornu Saigae Tataricae powder is broken into fine powder, add 8-12 times of water gaging and sodium hydroxide 2-6 weight portion, back hydrolysis filters, and medicinal residues add 3-6 times of water gaging and sodium hydroxide 1-2 weight portion, back hydrolysis, to almost molten entirely, filter merging filtrate; Being concentrated into relative density is 1.020-1.030; Lapis Chloriti, gypsum powder are broken into coarse powder, decoct 2 hours with 8-12 times of water gaging heating, filter, and medicinal residues add 6-10 times of water gaging and decocted 1-2 hour, filter, and merge filtrate twice, and being concentrated into relative density is 1.015-1.019; Artificial Calculus Bovis 3-6 doubly measured the 50-90% ethanol extraction 2-4 hour, and medicinal residues were doubly measured the 50-90% alcohol reflux 1-2 hour with 3-6, filtered, and merge and express liquid, decompression recycling ethanol, being concentrated into relative density is 1.020～1.030; Four flavors such as all the other Bulbus Fritillariae Ussuriensiss add 8-12 times of water gaging and decocted 1-3 hour, filter, medicinal residues add 3-6 times of water gaging heating and decocted 1-2 hour, filter merging filtrate, be concentrated into relative density 1.10, centrifugal, supernatant adds 2-5 and doubly measures 50-90% ethanol, stir evenly, leave standstill, get supernatant, filter, reclaim ethanol, be concentrated into relative density and be about 1.08; Get above-mentioned concentrated solution, add conventional adjuvant and make clinical acceptable forms, as oral liquid, capsule, granule, tablet, injection etc.
Wherein, a kind of preparation method of oral liquid is: take by weighing above-mentioned eight flavor medicines by weight portion disclosed by the invention, Cornu Saigae Tataricae powder is broken into fine powder, adds 10 times of water gagings and sodium hydroxide 4 weight portions, back hydrolysis 24 hours, filter, medicinal residues add 5 times of amounts: water and sodium hydroxide 2 weight portions, and back hydrolysis is to almost molten entirely, filter, merge filtrate twice: being concentrated into relative density is 20 ℃ of 1.020-1.030; Lapis Chloriti, gypsum powder are broken into coarse powder, decoct 2 hours with 10 times of water gaging heating, filter, and medicinal residues add 8 times of water gagings and decocted 1.5 hours, filter, and merge filtrate twice, and being concentrated into relative density is 1.015-1.019 (85 ℃); The artificial Calculus Bovis flows backwards with 5 times of amount 70% ethanol and extracted 3 hours, and medicinal residues were measured 70% alcohol reflux 1.5 hours with 5 times, filtered, and merge twice and express liquid, decompression recycling ethanol, being concentrated into relative density is 85 ℃ 1.020～1.030; Four flavors such as all the other Bulbus Fritillariae Ussuriensiss add 10 times of water gagings and decocted 2 hours, filter, medicinal residues add 5 times of water gaging heating and decocted 1.5 hours, filter, and merge filtrate twice, be concentrated into relative density and be about 1.10 (85 ℃), centrifugal, supernatant adds 4 times of amount ethanol, stir evenly, left standstill 48 hours, and got supernatant, filter, decompression recycling ethanol is concentrated into relative density and is about 1.08 (85 ℃); The stevioside who gets above-mentioned concentrated solution and 0.2% mixes, after stirring evenly, stirs evenly, and heated and boiled 20 minutes, cold preservation 48 hours filters, and filtrate adds the water adjustment, makes oral liquid.
Be the outer SARS resisting medicine primary dcreening operation result of the test of human body that invention is done below
One, experiment material
1, medicine: the oral liquid that above-mentioned Chinese medicine composition is made
2, cell: VeroE6 cell (African green monkey kidney cell)
3, virus: SARS virus, BJ-01 strain
4, culture fluid: the DMEM culture fluid that contains 10% hyclone
Two, three grades of laboratorys of experiment condition---bio-safety
Three, experimental procedure
1, CPE method (cellular morphology variation) is measured virus to VeroE6 cell half toxic concentration (TCID
50)
2, the CPE method is determined the non-toxic concn of medicine pair cell in conjunction with mtt assay
3, the maximal non-toxic concentration of medicine is to the evaluation of pesticide effectiveness of SARS virus
Four, experimental result
1, Bing Du TCID
50Be 10
-7
2, medicine is got 6 concentration and was carried out cytotoxic assay in promptly 1: 10,1: 100,1: 1000,1: 1 ten thousand and 1: 100 ten thousand, and every concentration is done 4 multiple holes, establishes the cell contrast simultaneously.The result shows that maximal non-toxic concentration is 1: 10000.
3, get 1: 10000 concentration liquid, do 4 multiple holes, observe inhibitory action SARS virus.Virus control and cell contrast are established in test.The result shows that this concentration has inhibitory action to SARS virus.
Five, conclusion
Under this experimental condition, oral liquid is 1: 10000 to the avirulence concentration of VeroE6 cell, and this concentration has complete inhibitory action to SARS virus.
The new purposes of a kind of Chinese medicine composition disclosed in this invention, the using method of this Chinese medicine composition is decided according to its dosage form.The active component that proves this Chinese medicine composition by experiment has the complete inhibition effect to SARS virus, and the control of " severe acute respiratory syndrome " is had significant meaning.
The specific embodiment
The following examples can make those skilled in the art further understand the present invention, rather than to the restriction of right of the present invention.
Embodiment 1.The application of following Chinese medicine composition in the medicine of preparation anti-SARS virus,
Cornu Saigae Tataricae 8 gram Bulbus Fritillariae Ussuriensiss 12 grams
Radix Et Rhizoma Rhei 20 gram Radix Scutellariaes 10 grams
Lapis Chloriti 10 gram Gypsum Fibrosum 15 grams
Artificial Calculus Bovis 8 gram Radix Glycyrrhizaes 20 grams
Extract active ingredient in the above-mentioned raw materials by conventional method, add conventional medical accessory again, make capsule, be used for anti-SARS virus.
Embodiment 2.The application of following Chinese medicine composition in the medicine of preparation anti-SARS virus,
Cornu Saigae Tataricae 9.45 gram Bulbus Fritillariae Ussuriensiss 17.25 grams
Radix Et Rhizoma Rhei 31.50 gram Radix Scutellariaes 15.75 grams
Lapis Chloriti 15.75 gram Gypsum Fibrosum 23.62 grams
Artificial Calculus Bovis 9.45 gram Radix Glycyrrhizaes 31.50 grams
Take by weighing above-mentioned eight flavor medicines, Cornu Saigae Tataricae powder is broken into fine powder, add 10 times of water gagings and sodium hydroxide 4 weight portions, back hydrolysis 24 hours filters, and medicinal residues add 5 times of amounts: water and sodium hydroxide 2 weight portions, back hydrolysis, to almost molten entirely, filter, merge filtrate twice: being concentrated into relative density is 20 ℃ of 1.020-1.030; Lapis Chloriti, gypsum powder are broken into coarse powder, decoct 2 hours with 10 times of water gaging heating, filter, and medicinal residues add 8 times of water gagings and decocted 1.5 hours, filter, and merge filtrate twice, and being concentrated into relative density is 1.015-1.019 (85 ℃); The artificial Calculus Bovis flows backwards with 5 times of amount 70% ethanol and extracted 3 hours, and medicinal residues were measured 70% alcohol reflux 1.5 hours with 5 times, filtered, and merge twice and express liquid, decompression recycling ethanol, being concentrated into relative density is 85 ℃ 1.020～1.030; Four flavors such as all the other Bulbus Fritillariae Ussuriensiss add 10 times of water gagings and decocted 2 hours, filter, medicinal residues add 5 times of water gaging heating and decocted 1.5 hours, filter, and merge filtrate twice, be concentrated into relative density and be about 1.10 (85 ℃), centrifugal, supernatant adds 4 times of amount ethanol, stir evenly, left standstill 48 hours, and got supernatant, filter, decompression recycling ethanol is concentrated into relative density and is about 1.08 (85 ℃); The stevioside who gets above-mentioned concentrated solution and 0.2% mixes, after stirring evenly, stirs evenly, and heated and boiled 20 minutes, cold preservation 48 hours filters, and filtrate adds the water adjustment, makes oral liquid.It is mould to be used for anti-SARS disease.
Embodiment 3.The application of following Chinese medicine composition in the medicine of preparation anti-SARS virus,
Cornu Saigae Tataricae 12 gram Bulbus Fritillariae Ussuriensiss 20 grams
Radix Et Rhizoma Rhei 35 gram Radix Scutellariaes 18 grams
Lapis Chloriti 18 gram Gypsum Fibrosum 25 grams
Artificial Calculus Bovis 12 gram Radix Glycyrrhizaes 35 grams
Get above eight flavors, Cornu Saigae Tataricae powder is broken into fine powder, adds 8 times of water gagings and sodium hydroxide 6 grams, and back hydrolysis filters, and medicinal residues add 6 times of water gagings and sodium hydroxide 1 gram, and back hydrolysis to almost molten entirely, filters merging filtrate; Being concentrated into relative density is 1.020; Lapis Chloriti, gypsum powder are broken into coarse powder, decoct 2 hours with 12 times of water gaging heating, filter, and medicinal residues add 10 times of water gagings and decocted 2 hours, filter, and merge filtrate twice, and being concentrated into relative density is 1.015; 6 times of amounts of artificial Calculus Bovis, 60% ethanol extraction 4 hours, medicinal residues were measured 90% alcohol reflux 2 hours with 6 times, filtered, and merge and express liquid, decompression recycling ethanol, being concentrated into relative density is 1.020; Four flavors such as all the other Bulbus Fritillariae Ussuriensiss add 8 times of water gagings and decocted 3 hours, filter, medicinal residues add 6 times of water gaging heating and decocted 2 hours, filter merging filtrate, be concentrated into relative density 1.10, centrifugal, supernatant adds 5 times of amount 90% ethanol, stir evenly, leave standstill, get supernatant, filter, reclaim ethanol, be concentrated into relative density and be about 1.08; Get above-mentioned concentrated solution, add conventional adjuvant and make tablet.
Embodiment 4.The application of following Chinese medicine composition in the medicine of preparation anti-SARS virus,
Cornu Saigae Tataricae 10 gram Bulbus Fritillariae Ussuriensiss 15 grams
Radix Et Rhizoma Rhei 30 gram Radix Scutellariaes 15 grams
Lapis Chloriti 18 gram Gypsum Fibrosum 20 grams
Artificial Calculus Bovis 10 gram Radix Glycyrrhizaes 25 grams
Get above eight flavors, Cornu Saigae Tataricae powder is broken into fine powder, adds 8 times of water gagings and sodium hydroxide 6 grams, and back hydrolysis filters, and medicinal residues add 6 times of water gagings and sodium hydroxide 1 gram, and back hydrolysis to almost molten entirely, filters merging filtrate; Being concentrated into relative density is 1.030; Lapis Chloriti, gypsum powder are broken into coarse powder, decoct 2 hours with 12 times of water gaging heating, filter, and medicinal residues add 10 times of water gagings and decocted 2 hours, filter, and merge filtrate twice, and being concentrated into relative density is 1.019; 6 times of amounts of artificial Calculus Bovis, 80% ethanol extraction 4 hours, medicinal residues were measured 90% alcohol reflux 2 hours with 6 times, filtered, and merge and express liquid, decompression recycling ethanol, being concentrated into relative density is 1.030; Four flavors such as all the other Bulbus Fritillariae Ussuriensiss add 8 times of water gagings and decocted 3 hours, filter, medicinal residues add 6 times of water gaging heating and decocted 2 hours, filter merging filtrate, be concentrated into relative density 1.10, centrifugal, supernatant adds 5 times of amount 85% ethanol, stir evenly, leave standstill, get supernatant, filter, reclaim ethanol, be concentrated into relative density and be about 1.08; Get above-mentioned concentrated solution, add conventional adjuvant and make injection.
Claims (4)
1, the application of following Chinese medicine composition in the medicine of preparation anti-SARS virus,
Cornu Saigae Tataricae 8-12 weight portion Bulbus Fritillariae Ussuriensis 12-20 weight portion
Radix Et Rhizoma Rhei 20-35 weight portion Radix Scutellariae 10-18 weight portion
Lapis Chloriti 10-18 weight portion Gypsum Fibrosum 15-25 weight portion
Artificial Calculus Bovis 8-12 weight portion Radix Glycyrrhizae 20-35 weight portion
2, the application of Chinese medicine composition according to claim 1 in the medicine of preparation anti-SARS virus is characterized in that this Chinese medicine composition is,
Cornu Saigae Tataricae 9.45 weight portion Bulbus Fritillariae Ussuriensiss 17.25 weight portions
Radix Et Rhizoma Rhei 31.50 weight portion Radix Scutellariaes 15.75 weight portions
Lapis Chloriti 15.75 weight portion Gypsum Fibrosum 23.62 weight portions
Artificial Calculus Bovis 9.45 weight portion Radix Glycyrrhizaes 31.50 weight portions
3, the application of Chinese medicine composition according to claim 1 and 2 in the medicine of preparation anti-SARS virus is characterized in that described medicine is said any dosage form on the pharmaceutics.
4, the application of Chinese medicine composition according to claim 3 in the medicine of preparation anti-SARS virus is characterized in that described medicine is an oral liquid.
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| CNB031323871A CN1239188C (en) | 2003-08-16 | 2003-08-16 | Use of Chinese medicine composition in preparing medicine for anti SARS virus |
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| CNB031323871A CN1239188C (en) | 2003-08-16 | 2003-08-16 | Use of Chinese medicine composition in preparing medicine for anti SARS virus |
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| CN1488380A true CN1488380A (en) | 2004-04-14 |
| CN1239188C CN1239188C (en) | 2006-02-01 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991763B (en) * | 2009-08-20 | 2012-05-23 | 江苏康缘药业股份有限公司 | Application of traditional Chinese medicinal composition to preparing medicaments for resisting influenza virus |
| CN101721604B (en) * | 2008-10-28 | 2012-11-28 | 江苏康缘药业股份有限公司 | Application of traditional Chinese medicine composition in preparing medicine for treating hand, mouth and foot diseases |
| CN108704058A (en) * | 2018-08-21 | 2018-10-26 | 江苏康缘药业股份有限公司 | A kind of composition and its preparation method and application with treatment pharyngitis |
| CN113288967A (en) * | 2020-02-21 | 2021-08-24 | 江苏康缘药业股份有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for treating or preventing coronavirus infection |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101327294B (en) * | 2007-06-22 | 2010-12-15 | 江苏康缘药业股份有限公司 | Chinese medicinal composition for treating common cold, acute and chronic tracheitis and preparation method thereof |
-
2003
- 2003-08-16 CN CNB031323871A patent/CN1239188C/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101721604B (en) * | 2008-10-28 | 2012-11-28 | 江苏康缘药业股份有限公司 | Application of traditional Chinese medicine composition in preparing medicine for treating hand, mouth and foot diseases |
| CN101991763B (en) * | 2009-08-20 | 2012-05-23 | 江苏康缘药业股份有限公司 | Application of traditional Chinese medicinal composition to preparing medicaments for resisting influenza virus |
| CN108704058A (en) * | 2018-08-21 | 2018-10-26 | 江苏康缘药业股份有限公司 | A kind of composition and its preparation method and application with treatment pharyngitis |
| CN113288967A (en) * | 2020-02-21 | 2021-08-24 | 江苏康缘药业股份有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for treating or preventing coronavirus infection |
| WO2021164399A1 (en) * | 2020-02-21 | 2021-08-26 | 江苏康缘药业股份有限公司 | Application of traditional chinese medicine composition in preparation of drugs for treating or preventing coronavirus infection |
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| CN1239188C (en) | 2006-02-01 |
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