CN1483312A - Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line - Google Patents
Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line Download PDFInfo
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- CN1483312A CN1483312A CNA031420974A CN03142097A CN1483312A CN 1483312 A CN1483312 A CN 1483312A CN A031420974 A CNA031420974 A CN A031420974A CN 03142097 A CN03142097 A CN 03142097A CN 1483312 A CN1483312 A CN 1483312A
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- seed
- rape
- male sterility
- minus green
- chlorisis
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- 206010021929 Infertility male Diseases 0.000 title claims abstract description 20
- 208000007466 Male Infertility Diseases 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000009395 breeding Methods 0.000 title claims abstract description 5
- 230000001488 breeding effect Effects 0.000 title claims abstract description 5
- 210000000805 cytoplasm Anatomy 0.000 title abstract description 8
- 235000011293 Brassica napus Nutrition 0.000 title 1
- 240000008100 Brassica rapa Species 0.000 title 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 title 1
- 230000007812 deficiency Effects 0.000 title 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims abstract description 20
- 241000220259 Raphanus Species 0.000 claims abstract description 19
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims abstract description 19
- 240000007124 Brassica oleracea Species 0.000 claims abstract description 18
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims abstract description 18
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims abstract description 18
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- 240000002791 Brassica napus Species 0.000 claims abstract description 12
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims abstract description 12
- 230000029580 nectary development Effects 0.000 claims abstract description 10
- 230000005855 radiation Effects 0.000 claims abstract description 6
- 230000001086 cytosolic effect Effects 0.000 claims description 21
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 16
- 229930002875 chlorophyll Natural products 0.000 claims description 14
- 235000019804 chlorophyll Nutrition 0.000 claims description 14
- 230000008859 change Effects 0.000 claims description 2
- 208000000509 infertility Diseases 0.000 abstract description 7
- 230000036512 infertility Effects 0.000 abstract description 7
- 208000021267 infertility disease Diseases 0.000 abstract description 7
- 238000009396 hybridization Methods 0.000 abstract description 2
- QDOXWKRWXJOMAK-UHFFFAOYSA-N dichromium trioxide Chemical compound O=[Cr]O[Cr]=O QDOXWKRWXJOMAK-UHFFFAOYSA-N 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 230000008901 benefit Effects 0.000 description 4
- 230000009466 transformation Effects 0.000 description 3
- 241000256844 Apis mellifera Species 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- 241001674939 Caulanthus Species 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 229930002868 chlorophyll a Natural products 0.000 description 2
- 229930002869 chlorophyll b Natural products 0.000 description 2
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 244000178993 Brassica juncea Species 0.000 description 1
- 235000005855 Brassica juncea var. subintegrifolia Nutrition 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000010211 insect pollination Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses a method for breeding cabbage rape non-chlorisis radish cytoplasm male sterility line, and is characterized by that it adopts 60 Co-gamma as radiation source to treat the cabbage rape radish cytoplasm male sterility type cytoplasm mate sterility line (Ogu cms) seed whose leaf has chlorisis and nectary development is poor, and makes the non-chlorisis and nectary development is poor, and makes the non-chlorisis and nectary development is poor, and makes the non-chlorisis mutated plant produced by Ogu cms seed and non-chlorisis conventional rape seed undergo the processes of hybridization and backcross breeding so as to obtain the invented product with normal leaf green, good nectary development and stable sterility.
Description
Technical field:
The invention belongs to the selection of radish cytoplasmic male sterility, the selection of the non-minus green radish cytoplasmic male sterility of a kind of cabbage type rape of more specifically saying so.
Background technology:
Rape is the main oil crop of China, and on rape heterosis production utilized, China had found good Pori horse (POL cms Fu Ting 1981), Shan 2A (Lee hall honor 1980) cytoplasmic male sterility type.(" breeding of cross-bred rape and utilization " Hubei science tech publishing house nineteen ninety-five), these sterile types obtained very big economic and social benefit in the popularization aspect the rape hybridization, but also exist many weak points: (1) responsive to temperature, sterility is not thorough, seriously influences the yield heterosis of cross-bred rape; As temperature was seriously unusual in recent years, because it all is Pori horse, Shan 2A cytoplasmic male sterility type that the cross-bred rape of using is gone up in China's production now, under low temperature condition, a large amount of trace-pollens generally appear in these male sterile lines, make each province and city hybrid rape seed quality sharply descend, cause enormous economic loss to agricultural production.(2) the maintenance source is narrow, and good maintenance line is more difficult to be looked for, and upgrades with new combination thereby be difficult to obtain strong advantage combination.(3) disease resistance is poor; (4) cytoplasm is single; POL cms and Shan 2A cms belong to a kind of kytoplasm type together, cause the large tracts of land cross-bred rape production of present China all to come from a kind of sterile cytoplasm, and this has constituted a kind of potential threat to China's rape production.
Aspect expanding and creating new sterile type, the whole world all arrives attention diversion on the cabbage type rape Ogura male sterile with radish cytoplasm type (Ogura cms) at present, Bannerot (1977) Bonnet (1975～1977) guides to France to Ogu cms, and with the repeated backcross method Wild cabbage type rape kernet is imported among the Japanese trailing plants crawl male sterile line Ogu cms, breed the cabbage type rape male sterile line.This male sterile line flower pesticide serious degradation, WUHUAFEN, sterility are not influenced by temperature condition, and its sterility is highly stable, thorough, is not subjected to the influence of environmental condition; Disease resistance is strong; The maintenance source is wide, and existing rape variety all is its good maintenance line; Far away with the rape genetic distance, this sterile source transformation is used in rape, easily finds the crossbreed combination of powerful coordinate force; Therefore has great economic worth.
Though cabbage type rape Ogura male sterile with radish cytoplasm type (Ogura cms) has above-mentioned advantage, the serious minus green of this material (temperature≤12 ℃ time) under low-temperature condition, the field whole strain of performance seedling is yellow; Nectary development is bad, influences insect pollination; These shortcomings cause this kind cabbage type rape radish cytoplasmic male sterile line finally can't be used on producing.
Summary of the invention:
At the technical problem that exists in the prior art, the invention provides the selection of a kind of not minus green, the non-minus green radish cytoplasmic male sterility of the normal cabbage type rape of nectary development.
The present invention is to realize by such technical scheme for reaching above purpose: the selection of the non-minus green radish cytoplasmic male sterility of a kind of cabbage type rape is provided, and described method is to adopt
60Co-γ is as radiation source, handle blade minus green, bad cabbage type rape radish cytoplasmic male sterility type cytoplasmic male sterile line (Ogu cms) seed of nectary development, Ogu cms seed is produced the seed selection that the normal rapeseed kind of the plant of non-minus green sudden change and non-minus green is hybridized, backcrossed.
Described selection may further comprise the steps successively:
1) selects 5000～8000 of the dry seeds of described Ogu cms for use, use
60The Co-gamma-rays carries out irradiation as irradiation bomb, and irradiation dose is 2500Gy～3000Gy (gray(Gy)), obtains first generation seed M
1
2) with M
1Sow, select the light individual plant of 60～150 strain radiation damagies, the normal rapeseed kind pollen of awarding described non-minus green is hybridized, and obtains second generation seed M
2
3) with M
2Sow, select wherein the not individual plant of minus green, award same rape variety pollen and backcross, obtain third generation seed M
3
4) with M
3Sow, select wherein 50～90 high individual plants of chlorophyll content, award same rape variety pollen and proceed to backcross, produce the 4th generation seed M
4
5) at M
4And after from generation to generation in, according to 4) method repeat, up to selecting chlorophyll content 〉=as the non-minus green radish cytoplasmic male sterility of cabbage type rape of parent's normal rapeseed kind.
The normal rapeseed kind of described non-minus green be in two No. 2.
Adopt the non-minus green radish cytoplasmic male sterility of cabbage type rape of said method seed selection, have the following advantages:
1., the whole strain leaf look of seedling is normal green, seedling stage growing way strong, under the weather conditions of field low temperature growing way the same with the normal rapeseed kind, can put into production use;
2., nectary development is normal, because rape is the honeybee matchmaker crop that pollinates has only the field honeybee producting honey normal, could improve the production of hybrid seeds amount and the production output of rape seed greatly;
3., flower pesticide serious degradation, WUHUAFEN, the sterility that keeps original radish cytoplasmic male sterility (Ogu cms) do not influenced by temperature condition, sterility is highly stable and good characteristic completely;
4., the maintenance source is wide, the existing conventional rape variety all is its good maintenance line.
5., belong to Cruciferae together, very easily hybridize transformation with greengrocery crops such as Chinese cabbages, a variety of Chinese cabbage, green vegetables, broccoli, wild cabbage, leaf mustard, therefore can be easy to by transformation in brassicaceous vegetable, be the cytoplasmic male sterility source of cultivating the corresponding three line hybrid seed the best of above-mentioned greengrocery crop; And high efficiency, simple to operate.
Embodiment:
Embodiment 1:
1) selected the dry seed M05000 grain of the bad cabbage type rape radish cytoplasmic male sterility type cytoplasmic male sterile line (Ogu cms) of blade minus green, nectary development in 1996 for use, use
60The Co-gamma-rays carries out irradiation as irradiation bomb, and irradiation dose is 2800Gy, obtains first generation seed M
1
2) 1996-1997 is with M
1Sow, select the light plant of 60 strain radiation damagies, two No. 2 pollen were hybridized during performance was just relatively awarded near normal plant, obtained second generation seed M
2Simultaneously will in carry out selfing as backcross parent two No. 2;
3) 1997-1998 is with M
2Sow, select wherein plant to show as during the individual plant of minus green is not awarded two No. 2 pollen and backcross, obtain third generation seed M
3Simultaneously will in carry out selfing as backcross parent two No. 2;
4) 1998-1999 is with M
3Sow, the plant leaf look that produces is all similar with normal cultivar, get single-strain blade and survey chlorophyll content, in its chlorophyll content of testing result and the backcross parent pairs No. 2 similar, and it is still normal to run into microthermal climate (temperature≤12 ℃ time) Shi Yese in whole growing; Select the plant of good relatively 50 strains continue with in backcross for two No. 2, obtain the 4th generation seed M
4Simultaneously will in carry out selfing as backcross parent two No. 2;
5) 2000-2002 is with M
4Sow, the plant of generation is got single-strain blade and surveys chlorophyll content, in its chlorophyll content 〉=backcross parent of testing result two No. 2, select the plant of good relatively 50 strains continue with in backcross for two No. 2, obtain the 5th generation seed M
5Simultaneously will in carry out selfing as backcross parent two No. 2;
M
5Selection of the present invention produces the non-minus green radish cytoplasmic male sterility of a kind of cabbage type rape exactly; In two maintenance lines that carry out seed that selfing produced as this male sterile line for No. 2.
The male sterile line that provides the inventive method below and produced, as the contrast table of chlorophyll content in two No. 2, the male sterile line Ogu cms of minus green in the backcross parent.Experimental technique: above-mentioned three is seeded in sub-district between Tian Ding, selects to be in the blade of identical growthdevelopmental stage, vein is removed, a residue blade part is as chlorophyll measuring, the calculating of the mensuration of chlorophyll content and a, b value is adopted the Arnon method, with 751 spectrophotometric determination extinction values.
The result divides folding:
The chlorophyll content comparison sheet
| Material | Chlorophyll content | Chlorophyll a | Chlorophyll b | ??a/b |
| The male sterile line that the inventive method produced | ????0.7925 | ????0.6023 | ????0.1901 | ??3.168 |
| ??Ogu?cms | ????0.4137 | ????0.3309 | ????0.0828 | ??3.996 |
| In two No. 2 | ????0.7611 | ????0.5780 | ????0.1832 | ??3.155 |
Measurement result: male sterile line M of the present invention
5Chlorophyll content surpasses as among the parent two No. 2.Its chlorophyll content be in two No. 2 1.041 times, chlorophyll a content be in two No. 2 1.042 times, chlorophyll b content be in two No. 2 1.037 times.
The above is a specific embodiment of the present invention only, should be pointed out that for the person of ordinary skill of the art, can also make many modification and improvement, and all modification or improvement all should be considered as protection scope of the present invention.
Claims (3)
1, the selection of the non-minus green radish cytoplasmic male sterility of a kind of cabbage type rape is characterized in that: described method is to adopt
60Co-γ is as radiation source, handle blade minus green, bad cabbage type rape radish cytoplasmic male sterility type cytoplasmic male sterile line (Ogu cms) seed of nectary development, the plant of the non-minus green sudden change that Ogu cms seed is produced and the normal rapeseed kind of non-minus green is hybridized, backcross breeding.
2, selection according to claim 1 is characterized in that: described selection may further comprise the steps successively:
1) selects 5000～8000 of the dry seeds of described Ogu cms for use, use
60The Co-gamma-rays carries out irradiation as irradiation bomb, and irradiation dose is 2500Gy～3000Gy, obtains first generation seed M
1
2) with M
1Sow, select the light individual plant of 60～150 strain radiation damagies, the normal rapeseed kind pollen of awarding described non-minus green is hybridized, and obtains second generation seed M
2
3) with M
2Sow, select wherein the not individual plant of minus green, award same rape variety pollen and backcross, obtain third generation seed M
3
4) with M
3Sow, select wherein 50～90 high individual plants of chlorophyll content, award same rape variety pollen and proceed to backcross, produce the 4th generation seed M
4
5) at M
4And after from generation to generation in, according to 4) method repeat, up to selecting chlorophyll content 〉=as the non-minus green radish cytoplasmic male sterility of cabbage type rape of parent's normal rapeseed kind.
3, selection according to claim 1 is characterized in that: described normal rapeseed kind be in two No. 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03142097 CN1218628C (en) | 2003-08-02 | 2003-08-02 | Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03142097 CN1218628C (en) | 2003-08-02 | 2003-08-02 | Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1483312A true CN1483312A (en) | 2004-03-24 |
| CN1218628C CN1218628C (en) | 2005-09-14 |
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|---|---|---|---|
| CN 03142097 Expired - Fee Related CN1218628C (en) | 2003-08-02 | 2003-08-02 | Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071250A (en) * | 2010-06-25 | 2011-05-25 | 温州科技职业学院 | Broccoli Ogu cytoplasm quick identification kit and detection method thereof |
| CN102696474A (en) * | 2012-05-15 | 2012-10-03 | 西北农林科技大学 | Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish |
| CN108753763A (en) * | 2018-06-11 | 2018-11-06 | 北京市农林科学院 | A kind of selection of onion male sterile line |
-
2003
- 2003-08-02 CN CN 03142097 patent/CN1218628C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071250A (en) * | 2010-06-25 | 2011-05-25 | 温州科技职业学院 | Broccoli Ogu cytoplasm quick identification kit and detection method thereof |
| CN102696474A (en) * | 2012-05-15 | 2012-10-03 | 西北农林科技大学 | Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish |
| CN108753763A (en) * | 2018-06-11 | 2018-11-06 | 北京市农林科学院 | A kind of selection of onion male sterile line |
| CN108753763B (en) * | 2018-06-11 | 2021-09-07 | 北京市农林科学院 | Breeding method of onion male sterile line |
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| Publication number | Publication date |
|---|---|
| CN1218628C (en) | 2005-09-14 |
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