CN1463613A - Feed addictive - Google Patents
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- CN1463613A CN1463613A CN02112057A CN02112057A CN1463613A CN 1463613 A CN1463613 A CN 1463613A CN 02112057 A CN02112057 A CN 02112057A CN 02112057 A CN02112057 A CN 02112057A CN 1463613 A CN1463613 A CN 1463613A
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Abstract
The present invention is one kind of green feed additive. Probiotics strain is used in fermentation to produce beta-mannase; the beta-mannase is used in decomposing polymer carbohydrate to produce oligomannose and mannose; and the mixture of beta-mannase, oligomannose, mannose and probiotics as the green feed additive is finally produced. The said process is simple one-step process of producing the microecological preparation with high feed converting rate.
Description
One, technical field
The present invention relates to a kind of feed addictive, especially contain compound sugar, the feed addictive of probio and prepare the method for producing this feed addictive.
Two, background technology
People have realized that now in the medium-term and long-term abuse of antibiotics of feed, have not only caused weakening of letting animals feed resistance against diseases, the decline of meat egg product quality and meat flavour, and improved the probability of carrying resistance factor in the pathogen significantly.Therefore, not contain antibiotic feed addictive imperative in exploitation.Probiotics is subjected to people's attention as the antibiotic green feed additive of most possible replacement.It is generally acknowledged that probiotics comprises probio and prebiotics two classes.Fowl poultry kind commonly used comprises Bacillus, Lactobacillus, Bifidobacterium, the part bacterial strain in streptococcus and the photosynthetic cells with probio.Add probio in the feed and can suppress the breeding of pathogen in animal intestinal competitively, and produce benefit materials such as vitamin digestive ferment, absorbed by animal and utilize.
Prebiotics is meant not a series of carbohydrate by animal body digested.Usually form by 2-10 unit identical or inequality.Can optionally promote the activity and the growth and breeding of profitable strain in the intestines, promote the healthy and somatotrophic effect of host thereby reach.The prebiotics that fowl poultry kind is used comprises FOS, Oligomeric manna sugar, polytypes such as oligoisomaltose.Wherein Oligomeric manna sugar and monose molecule mannose thereof are subject to people's attention with its unique physiologically active, become the antibiotic novel fodder additive of most possible replacement.The mechanism of action of mannose and Oligomeric manna sugar comprises: 1. can be competitively and pathogen, Escherichia coli for example, salmonella is treated the receptors bind of bacterium surface, thereby stops the receptors bind of pathogen and intestines wall surface, accelerate the speed that bacterium excretes from enteron aisle, reduce the breeding of bacterium.So in the chicken feed, add the infection that mannose can reduce salmonella in the chicken enteron aisle effectively (US6080442, US6374220).2. can stimulate and promote the immunity function of animal.(" Chinese feed " 2001,6,20).Interpolation Oligomeric manna sugar or mannose can improve production performance (" feed wide-angle ", 2001,20,23) and the immunologic function (" Chinese animal doctor's journal ", 2000,20,257) of sow and piglet in sow feed.3. can be used as the growth (" Tianjin microorganism " 1996,2,1) of bifidus factor promotion Bifidobacterium.
State of Zhao's fine jades etc. are reported in " Chinese feed " 5,14: the 'beta '-mannase that adds proper level in laying hen can improve egg laying performance and feed conversion rate effectively, and 'beta '-mannase is β-Glucomannan enzyme.People such as Pettey are reported in " animal science magazine, Journal of AnimalScience " 80,1012 and add the meat feed ratio that 'beta '-mannase can be promoted piglet effectively in the pig starter feed.And further add at fattening stage and not only can improve daily gain, can also increase the lean meat ratio.Chinese patent prospectus CN1264547A discloses a kind of additive for microbe feedstuff, is to be that main component adds excipient again with the normal flora, makes it to be processed into the powdery additive for microbe feedstuff.This feed addictive contains probio and easy to use, but effect is single, costs an arm and a leg.
Chinese patent prospectus CN1262880A discloses a kind of composite prebiotics feed additive, is made up of probiotic and compound sugar, and the promptly top said probio of the probiotic here is to be added with compound sugar on the basis of probio.Though it makes the probio function more effectively powerful and since the compound sugar that added this as extract, cost is higher, makes the cost of this feed addictive also very high.This prebiotics feed additive does not contain 'beta '-mannase simultaneously, does not have help to improving food conversion ratio, and its effect is single.
U.S. Patent Publication specification US 5429828 and US 6162473 disclose the method for adding 'beta '-mannase in feed.And emphasize that especially adding 'beta '-mannase in the corn bean-dregs feed can improve food conversion ratio effectively, and decompose the 'beta '-mannase that contains in the feed dregs of beans, improve the nutritive value of feed.But since the 'beta '-mannase that added this as the enzyme powder behind the purifying, cost is higher, has limited the use of this feed addictive.
U.S. Patent Publication specification US 6374220 discloses and has a kind ofly partly decomposed beta-mannase in the coconut dregs of rice with enzyme process and obtain mannose and as the method for feed addictive, find to add the enzymolysis coconut dregs of rice that contain mannose and can reduce the content of salmonella in the chicken enteron aisle effectively in feed in addition.But the product price that this method that directly adds enzyme or mannose and Oligomeric manna sugar in feed is produced is somewhat expensive, and does not contain probio as feed addictive, and its effect is single.Three, summary of the invention
The method that the technical problem to be solved in the present invention provides a kind of green feed additive that can promote the fowl poultry kind healthy growth and produces this green feed additive of preparation is produced green feed additive cost high price costliness, time-consuming shortcoming to solve in the prior art.For this reason, the present invention takes following technical scheme:
A kind of feed addictive, adopt following method to make: the probiotics strain fermentation, produce 'beta '-mannase, the poly carbohydrate that can produce mannan-oligosaccharides and mannose after described 'beta '-mannase decomposition is decomposed generates mannan-oligosaccharides and mannose, generate at last and contain 'beta '-mannase, mannan-oligosaccharides, the mixture of mannose and probio is as feed addictive; This method comprises the steps:
(1) select the nitrogenous source of probio, suitable selected probio growth to mix respectively, add the poly carbohydrate mixed culture fermentation that can produce mannan-oligosaccharides and mannose after being decomposed again, described poly carbohydrate dosage is 1g/L-20g/L, and the inoculum concentration amount of probio described in the described zymotic fluid is controlled between the 1%-10%;
(2) with acid or alkali zymotic fluid pH is transferred to 5.5-7.5, temperature is controlled at 25 ℃-42 ℃, the beginning fermentation reaction;
(3) till the fermentation termination time was broken down into compound sugar with the poly carbohydrate, the time was 8-72 hour;
When (4) fermentation stopped, zymotic fluid was final products.
Described feed addictive, described probio contains one of following formula at least: 1. bacillus; 2. aspergillus.
Described bacillus can be a 1. 2. 3. bacillus lentus of bacillus licheniformis of bacillus subtilis of one of following formula.
Described aspergillus can be 1. 2. aspergillus oryzae of aspergillus niger of one of following formula.
Described poly carbohydrate contains one of following formula at least: 1. konjaku flour; 2. carob; 3. melon glue.
Further, described feed addictive, its preferred poly carbohydrate is a konjaku flour, end product contains mannose and mannan-oligosaccharides, 'beta '-mannase.
Further again, described feed addictive, its probiotic bacteria is a bacillus subtilis, described poly carbohydrate is a konjaku flour, be that the bacillus subtilis strain fermentation produces 'beta '-mannase decomposition konjaku flour, end product contains mannose and mannan-oligosaccharides, 'beta '-mannase and bacillus subtilis.
Described feed addictive adds food grade defoamer during the fermentation.
Described feed addictive can directly join zymotic fluid in the drinking water of animal and drink, and the zymotic fluid in the time of also fermentation can being stopped suitably concentrates, and adds the feed excipient, makes granule or powdery with seasoning.Described excipient can be a certain component of animal feed or a certain component in the food additives.
The using method of described feed addictive: can add in the drinking water of animal and drink, also can admix in the feed feeding.
Feed addictive of the present invention contains following characteristics:
The first, the feed addictive among the present invention contains feeding probio and mannose, mannan-oligosaccharides, 'beta '-mannase simultaneously.
The second, the mannose that the feed addictive among the present invention contains, mannan-oligosaccharides decompose the poly carbohydrate by probio and make.
The 3rd, the probiotics fermention among the present invention can produce 'beta '-mannase and decompose, so the feed addictive among the present invention contains highly active 'beta '-mannase.
Among the present invention the probio of indication be refer in particular to drugs approved by FDA as the microorganism directly fed and the Ministry of Agriculture's in June, 1999 approval directly the feed level microbe additive of feeding animals record in the scope, all 'beta '-mannases that can produce decompose the probio of generation mannose, mannan-oligosaccharides all within the scope of application, as the bacillus subtilis in the bacillus, bacillus licheniformis and bacillus lentus.Aspergillus niger in the aspergillus and aspergillus oryzae for another example.The preferential bacterial strain that uses of the present invention is a Bacillus subtillis.
The preferred konjaku flour of employed poly carbohydrate among the present invention, konjaku flour are to refer in particular to white or the canescence particulate powder of making after konjak corm (the Amorpliorli allus Konjac) drying, and the quality of konjaku flour is not had special requirement.The dosage of mill taro powder in the zymotic fluid, from 1g/L to 20g/L can, as long as probio can grow fast, secrete the 'beta '-mannase of enough energy, divide the Glucomannan in the desintegrate taro powder.Other poly carbohydrate of probio growth that can promote to decompose mannose and mannan-oligosaccharides is all within the scope of application, as carob, melon glue.Carob or melon glue are the white powders that obtains from Sigma (Sigma Co.Ltd), and the quality of carob or melon glue is not had special requirement.Selected nitrogenous source is meant the component of the culture medium of the probio growth that can promote to decompose mannose and mannan-oligosaccharides among the present invention, comprising: 1. corn steep liquor; 2. peptone; 3. yeast extract; 4. micro-; 5. phosphate buffer; 6. vitamin; 7. amino acid; 8. beef extract etc. can increase and reconfigures according to different probios growths and the requirement of decomposing the poly carbohydrate.Can use food grade defoamer according to the growth requirement during the fermentation.
PH value can be adjusted to suitable level with bronsted lowry acids and bases bronsted lowry according to the requirement of different probiotic strains in the sweat.Usually be controlled at 5.5-7.5.
Temperature control in the sweat can be according to the requirement adjustment of different probiotics strains.Generally between 25 ℃～42 ℃.The fermentation termination time resolves into till mannose, the mannan-oligosaccharides with carbon source, is generally 8-72 hour.
The content of probio is distinguished to some extent according to different strains in the zymotic fluid, is shaped between 1%10% but the inoculum concentration of probio is controlled, and general 'beta '-mannase content reaches 70 units per ml.
Zymotic fluid when fermentation stops is final products.Also can carry out as required suitably concentrating.And can sneak into suitable vehicle, make fine granularity or powdery with dry method.Various components that excipient can be selected for use in the animal feed to be added or the component in the food additives, the embedding medium that is added in the time of also can being drying.
The probiotics that contains mannose, mannan-oligosaccharides and probio related among the present invention can join laying hen with liquid form, fryer, and dove, duck, goose, pig, ox in the drinking-water of sheep, or gavages or admixes in the feed feeding to animal.Also can add premixing feedstuff to, in the complete feed with dried particle or powder-form.
The present invention also provides the preparation method of feed addictive: the probiotics strain fermentation, produce 'beta '-mannase, the poly carbohydrate that can produce mannan-oligosaccharides and mannose after described 'beta '-mannase decomposition is decomposed generates mannan-oligosaccharides and mannose, generate at last and contain 'beta '-mannase, mannan-oligosaccharides, the mixture of mannose and probio is as feed addictive; This method comprises the steps:
1. select probio respectively, be fit to the nitrogenous source mixing that selected probio grows, add the poly carbohydrate mixed culture fermentation that can produce mannan-oligosaccharides and mannose after being decomposed again, described poly carbohydrate dosage is 1g/L-20g/L, and the inoculum concentration of probio is controlled between the 1%-10% described in the described zymotic fluid;
2. with acid or alkali zymotic fluid pH is transferred to 5.5-7.5, temperature is controlled at 25 ℃-42 ℃, the beginning fermentation reaction;
3. ferment till the termination time is broken down into compound sugar with the poly carbohydrate, the time is 8-72 hour;
When 4. fermentation stopped, zymotic fluid was final products.
Actual effect of the present invention is to estimate with the fermentation content of reducing sugar that stop buffer was produced, as with 3, the total reducing sugars content of mannose and mannan-oligosaccharides in the 5-2 nitro-salicylic acid colorimetric method for determining fermentation stop buffer, and calculating beta-mannase enzymatic activity, measure in the fermentation stop buffer mannose content or measure mannose with high-efficient liquid phase technique, in addition with ultraviolet spectrophotometry at 600 millimeters growing states of locating to measure probio of wavelength.
The present invention has the following advantages with respect to prior micro-ecological preparation:
1, manufacture technology is simple, can produce to contain profitable probliotics the full price probiotics of mannose and mannan-oligosaccharides and 'beta '-mannase by one-step fermentation.Do not need to purify, refining.
2, cost reduces greatly, and the raw material wide material sources are fit to mass industrialized production.
3, saving of work and time, the feed conversion rate height.
Four, description of drawings
Fig. 1 is the growth curve of Bacillus subtillis in containing 0.1% konjaku flour culture medium among the present invention.
Five, the specific embodiment
The poly carbohydrate is selected konjaku flour for use, and probio is selected Bacillus subtillis for use, and nitrogenous source is selected peptone and beef extract for use.Utilize 'beta '-mannase that Bacillus subtillis self produced to decompose konjaku flour by fermentation process and produce mannose, mannan-oligosaccharides and making.Contain mannose in the final products, mannan-oligosaccharides, 'beta '-mannase and Bacillus subtillis.The konjaku flour that present embodiment uses is the konjaku powder that contains 80% above β-Glucomannan, and wherein contents of starch accounts for 2.4%.
The preparation method: (content: cultivation 1g/L) wherein in containing 0.1% mill taro powder for 5% Bacillus subtillis XL13 bacterial classification inoculation.Culture medium is selected for use: beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L.PH value 7.0～7.2; 37 ℃ of shaking tables were cultivated 24 hours.Locate to measure growth with ultraviolet specrophotometer 600 millimeters of wavelength, the growth curve of Bacillus subtillis XL13 in mill taro culture medium as shown in Figure 1.The fermentation stop buffer detects mannose, mannan-oligosaccharides content with HPLC, the analysis condition of HPLC: SHODEX KS-801 sugar post, and length 300mm * 8mm, 700 ℃ of column temperatures, the 1ul/min flow velocity, the differential detector (RID, 350C).Mannose content reaches 0.17mg/ml in 24 hours fermentation stop buffers.
Probio, poly carbohydrate, nitrogenous source are selected as embodiment 1, and the preparation method is as follows: 5% Bacillus subtillis XL13 is seeded in and contains 2.0% mill taro powder (content: in culture medium 20g/L).Media components as described in example 1 above.Sampling in 0 hour, 8 hours, 27 hours, 62 hours, 72 hours, with 3, content of reducing sugar in the 5-2 nitro-salicylic acid colorimetric method for determining zymotic fluid (AppliedMicrobiology and Biotechnology 26,323).Enzymatic activity is defined as per minute, and to discharge the required enzyme amount of reduced sugar that 1 μ mol is equivalent to the D-mannose be 1 enzyme activity unit.The results are shown in Table 1.Enzyme activity is up to 162IU/ml.
Table 1:
Embodiment 3
| Bacterial strain | Fermentation time (hour) | Growth (OD600) | Content of reducing sugar (mg/ml) | Enzyme activity (IU/ml) |
| 5% withered grass bud bag bacillus XL13 | ?0 | ?0.14 | ?0.43 | ?- |
| ?8 | ?0.62 | ?4.57 | ?- | |
| ?23 | ?1.34 | ?7.12 | ?162.0 | |
| ?62 | ?1.23 | ?3.27 | ?67.3 | |
| ?72 | ?1.28 | ?3.16 | ?- |
Probio, poly carbohydrate, carbon source are selected as embodiment 1, and the preparation method is as follows: 5% bacillus licheniformis XL42 is seeded in the culture medium that contains 2.0% mill taro powder.Described in media components such as the embodiment one.Sampling in 0 hour, 8 hours, 27 hours, 62 hours, 72 hours, with 3, content of reducing sugar in the 5-2 nitro-salicylic acid colorimetric method for determining zymotic fluid (AppliedMicrobiology and Biotechnology 26,323).Enzymatic activity is defined as per minute, and to discharge the required enzyme amount of reduced sugar that 1 μ mol is equivalent to the D-mannose be 1 enzyme activity unit.The results are shown in Table 2.Enzyme activity is up to 38IU/ml.
Table 2:
Embodiment 4
| Bacterial strain | Fermentation time (hour) | Growth (OD600) | Content of reducing sugar (mg/ml) | Enzyme activity (IU/ml) |
| 5% lichens bud bag bacillus XL42 | ?0 | ?0.15 | ?0.43 | ?- |
| ?8 | ?0.84 | ?0.66 | ?- | |
| ?23 | ?1.50 | ?5.61 | ?38.0 | |
| ?62 | ?1.75 | ?3.66 | ?20.7 | |
| ?72 | ?1.73 | ?3.36 | ?- |
Probio, poly carbohydrate, carbon source are selected as embodiment 1, and the preparation method is as follows: 5% Bacillus lentus XL12 is seeded in the culture medium that contains 2.0% mill taro powder.Described in media components such as the example one.Sampling in 0 hour, 8 hours, 27 hours, 62 hours, 72 hours, with 3, content of reducing sugar in the 5-2 nitro-salicylic acid colorimetric method for determining zymotic fluid (AppliedMicrobiology and Biotechnology 26,323).Enzymatic activity is defined as per minute, and to discharge the required enzyme amount of reduced sugar that 1 μ mol is equivalent to the D-mannose be 1 enzyme activity unit.The results are shown in Table 1.Enzyme activity is up to 83.3IU/ml.Table 3:
| Bacterial strain | Fermentation time (hour) | Growth (OD600) | Content of reducing sugar (mg/ml) | Enzyme activity (IU/ml) |
| Slow bud bag bacillus XL12 | ?0 | ?0.14 | ?0.43 | ??- |
| ?8 | ?0.67 | ?0.60 | ??- | |
| ?23 | ?1.24 | ?6.21 | ??83.3 | |
| ?62 | ?1.62 | ?3.80 | ??77.6 | |
| ?72 | ?1.652 | ?3.86 | ??- |
Embodiment 6
The poly carbohydrate is selected carob for use, and probio is selected Bacillus subtillis for use, nitrogenous source such as embodiment 1.The carob that present embodiment uses is the white powder that contains 95% above purity that obtains from Sigma (Sigma Co.Ltd).The 'beta '-mannase that utilizes Bacillus subtillis self to be produced by fermentation process decomposes the final products that the substrate generation contains mannan-oligosaccharides 'beta '-mannase and Bacillus subtillis.The preparation method: (content: 10g/L) as the poly carbohydrate, other component in the fermentation medium as described in example 1 above for the carob with 1%; 5% Bacillus subtillis XL13 bacterial classification inoculation in the cultivation that contains carob wherein through cultivating 24 hours, is measured the growth and the content of reducing sugar of thalline on 37 ℃ of shaking tables.Get the growth and the content of reducing sugar of its fermentation zymotic fluid measurement in 0 hour, 8 hours, 24 hours, 72 hours thalline simultaneously.The results are shown in Table 4.Bacillus subtillis can utilize carob as the growth of poly carbohydrate, produces the B-mannase and decomposes the product that the substrate generation contains mannose, mannan-oligosaccharides
Table 4
| Bacterial strain | Poly carbon water | Fermentation time | Growth (OD 600) | Content of reducing sugar |
| Compound | (hour) | ??(mg/ml) | ||
| 5% | 1% carob | ?0 | ?0.02 | ?0.07 |
| ?8 | ?0.56 | ?2.04 | ||
| ?24 | ?1.34 | ?5.21 | ||
| ?72 | ?1.40 | ?3.01 |
Embodiment 7
The poly carbohydrate is selected melon glue for use, and probio is selected Bacillus subtillis for use, nitrogenous source such as embodiment 1.The melon glue that present embodiment uses is the white powder that contains 95% above purity that obtains from Sigma (Sigma Co.Ltd).The 'beta '-mannase decomposition substrate generation that utilizes Bacillus subtillis self to be produced by fermentation process contains mannan-oligosaccharides, 'beta '-mannase and Bacillus subtillis final products.The preparation method: (content: 1g/L) as the poly carbohydrate, other component in the fermentation medium as described in example 1 above for the melon glue with 1%; 5% Bacillus subtillis XL13 bacterial classification inoculation in the cultivation that contains melon glue wherein through cultivating 24 hours, is measured the growth and the content of reducing sugar of thalline on 37 ℃ of shaking tables.Get the growth and the content of reducing sugar of its fermentation zymotic fluid measurement in 0 hour, 8 hours, 24 hours, 72 hours thalline simultaneously.The results are shown in Table 4.Bacillus subtillis can utilize melon glue as the growth of poly carbohydrate, produces 'beta '-mannase and decomposes the product that the substrate generation contains mannose, mannan-oligosaccharides.Table 5
| Bacterial strain | The poly carbohydrate | Fermentation time (hour) | Growth (OD 600) | Content of reducing sugar (mg/ml) |
| 5% Bacillus subtillis XL13 | 1% melon glue | ?0 | ?0.03 | ?0.05 |
| ?8 | ?0.57 | ?0.23 | ||
| ?24 | ?1.45 | ?1.39 | ||
| ?72 | ?1.56 | ?2.67 |
Embodiment 8
The poly carbohydrate is selected konjaku flour for use, and probio is selected aspergillus niger for use, and nitrogenous source is selected yeast extract, peptone for use.The preparation method: 5% aspergillus niger XLn-1 kind liquid is inoculated in and contains 1% konjaku flour (content: in culture medium 10g/L).Other component in the culture medium comprises (gram/liter): yeast extract 5, peptone 5.After 37 ℃ are cultivated 72 hours, get supernatant and measure content of reducing sugar.The results are shown in Table 6.
Embodiment 9
The poly carbohydrate is selected carob for use, and probio is selected aspergillus niger for use, and the nitrogenous source nitrogenous source is selected yeast extract, peptone for use.The preparation method: 5% aspergillus niger XLn-1 kind liquid is inoculated in and contains 1% carob (content: in culture medium 10g/L).Other component in the culture medium comprises (gram/liter): yeast extract 5, peptone 5.After 37 ℃ are cultivated 72 hours, get supernatant and measure content of reducing sugar.The results are shown in Table 6.
The poly carbohydrate is selected melon glue for use, and probio is selected aspergillus niger for use, and the nitrogenous source nitrogenous source is selected yeast extract, peptone for use.The preparation method: 5% aspergillus niger XLn-1 kind liquid is inoculated in and contains 1% melon glue (content: in culture medium 10g/L).Other component in the culture medium comprises (gram/liter): yeast extract 5, peptone 5.After 37 ℃ are cultivated 72 hours, get supernatant and measure content of reducing sugar.The results are shown in Table 6.
Embodiment 11
The poly carbohydrate is selected konjaku flour for use, and probio is selected aspergillus oryzae for use, and nitrogenous source is selected yeast extract, peptone for use.The preparation method: 5% aspergillus oryzae XLn-1 kind liquid is inoculated in and contains 1% konjaku flour (content: in culture medium 10g/L).Other component in the culture medium comprises (gram/liter): yeast extract 5, peptone 5.After 37 ℃ are cultivated 72 hours, get supernatant and measure content of reducing sugar.The results are shown in Table 6.
Embodiment 12
The poly carbohydrate is selected carob for use, and probio is selected aspergillus oryzae for use, and the nitrogenous source nitrogenous source is selected yeast extract, peptone for use.The preparation method: 5% aspergillus oryzae XLn-1 kind liquid is inoculated in and contains 1% carob (content: in culture medium 10g/L).Other component in the culture medium comprises (gram/liter): yeast extract 5, peptone 5.After 37 ℃ are cultivated 72 hours, get supernatant and measure content of reducing sugar.The results are shown in Table 6.
Embodiment 13
The poly carbohydrate is selected melon glue for use, and probio is selected aspergillus oryzae for use, and the nitrogenous source nitrogenous source is selected yeast extract, peptone for use.The preparation method: 5% aspergillus oryzae XLn-1 kind liquid is inoculated in and contains 1% melon glue (content: in culture medium 10g/L).Other component in the culture medium comprises (gram/liter): yeast extract 5, peptone 5.After 37 ℃ are cultivated 72 hours, get supernatant and measure content of reducing sugar.The results are shown in Table 6.Table 6
| Embodiment | Probiotics strain | The poly carbohydrate | Content of reducing sugar (mg/ml) |
| Embodiment 8 | Aspergillus niger XLn-1 | 1% konjaku flour | 2.13 |
| Embodiment 9 | Aspergillus niger XLn-1 | 1% carob | 4.10 |
| | Aspergillus niger XLn-1 | 1% melon glue | 1.24 |
| Embodiment 11 | Aspergillus oryzae Xla-2 | 1% konjaku flour | 3.67 |
| Embodiment 12 | Aspergillus oryzae Xla-2 | 1% carob | 2.34 |
| Embodiment 13 | Aspergillus oryzae Xla-2 | 1% melon glue | 1.07 |
Claims (13)
1, a kind of feed addictive, it is characterized in that described feed addictive adopts following method to make: the probiotics strain fermentation, produce 'beta '-mannase, the poly carbohydrate that can produce mannan-oligosaccharides and mannose after described 'beta '-mannase decomposition is decomposed generates mannan-oligosaccharides and mannose, generates the mixture that contains 'beta '-mannase, mannan-oligosaccharides, mannose and probio at last and is used as feed addictive; This method comprises the steps:
(1) select the nitrogenous source of probio, suitable selected probio growth to mix respectively, add the poly carbohydrate mixed culture fermentation that can produce mannan-oligosaccharides and mannose after being decomposed again, described poly carbohydrate dosage is 1g/L-20g/L, and the inoculum concentration of probio is controlled between the 1%-10% described in the above-mentioned zymotic fluid;
(2) with acid or alkali zymotic fluid pH is transferred to 5.5-7.5, temperature is controlled at 25 ℃-42 ℃, begins fermentation;
(3) till the fermentation termination time was broken down into compound sugar with the poly carbohydrate, the time was 8-72 hour;
When (4) fermentation stopped, zymotic fluid was final products.
2, feed addictive as claimed in claim 1 is characterized in that described probio contains one of following formula at least: 1. 2. aspergillus of bacillus.
3, feed addictive as claimed in claim 2 is characterized in that described bacillus can be one of following formula: 1. 2. 3. bacillus lentus of bacillus licheniformis of bacillus subtilis.
4, feed addictive as claimed in claim 2 is characterized in that described aspergillus can be one of following formula: 1. 2. aspergillus oryzae of aspergillus niger
5, feed addictive as claimed in claim 1 is characterized in that described poly carbohydrate contains one of following formula at least: 1. 2. 3. melon glue of carob of konjaku flour.
6, feed addictive as claimed in claim 2 is characterized in that described poly carbohydrate contains one of following formula at least: 1. 2. 3. melon glue of carob of konjaku flour.
7, feed addictive as claimed in claim 3 is characterized in that described poly carbohydrate contains one of following formula at least: 1. 2. 3. melon glue of carob of konjaku flour.
8, feed addictive as claimed in claim 4 is characterized in that described poly carbohydrate contains one of following formula at least: 1. 2. 3. melon glue of carob of konjaku flour.
9, feed addictive as claimed in claim 1 is characterized in that described poly carbohydrate is a konjaku flour, and end product contains mannose and mannan-oligosaccharides, 'beta '-mannase.
10, feed addictive as claimed in claim 1, it is characterized in that described probio is a bacillus subtilis, described poly carbohydrate is a konjaku flour, be that the bacillus subtilis strain fermentation produces 'beta '-mannase decomposition konjaku flour, end product contains mannose and mannan-oligosaccharides, 'beta '-mannase and bacillus subtilis.
11, as the described feed addictive of claim 1-10, it is characterized in that adding during the fermentation food grade defoamer.
12, as the described feed addictive of claim 1-10, it is characterized in that zymotic fluid is suitably concentrated, add the feed excipient, make granule or powdery with seasoning.
13, a kind of method for preparing feed addictive as claimed in claim 1, it is characterized in that described preparation method is as follows: the probiotics strain fermentation, produce 'beta '-mannase, the poly carbohydrate that can produce mannan-oligosaccharides and mannose after described 'beta '-mannase decomposition is decomposed generates mannan-oligosaccharides and mannose, generates the mixture that contains 'beta '-mannase, mannan-oligosaccharides, mannose and probio at last and is used as feed addictive; This method comprises the steps:
(1) select the nitrogenous source of probio, suitable selected probio growth to mix respectively, add the poly carbohydrate mixed culture fermentation that can produce mannan-oligosaccharides and mannose after being decomposed again, described poly carbohydrate dosage is 1g/L-20g/L, and the inoculum concentration of probio is controlled between the 1%-10% described in the above-mentioned zymotic fluid;
(2) with acid or alkali zymotic fluid pH is transferred to 5.5-7.5, temperature is controlled at 25 ℃-42 ℃, begins fermentation;
(3) till the fermentation termination time was broken down into compound sugar with the poly carbohydrate, the time was 8-72 hour;
When (4) fermentation stopped, zymotic fluid was final products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021120579A CN1317968C (en) | 2002-06-11 | 2002-06-11 | Feed addictive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021120579A CN1317968C (en) | 2002-06-11 | 2002-06-11 | Feed addictive |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1463613A true CN1463613A (en) | 2003-12-31 |
| CN1317968C CN1317968C (en) | 2007-05-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021120579A Expired - Fee Related CN1317968C (en) | 2002-06-11 | 2002-06-11 | Feed addictive |
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| CN (1) | CN1317968C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100403921C (en) * | 2005-06-06 | 2008-07-23 | 北京新纪元三色生态科技有限公司 | A feed addictive, its preparation process and use |
| CN102178059A (en) * | 2011-05-13 | 2011-09-14 | 青岛诺和诺康生物技术有限公司 | Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof |
| CN103583840A (en) * | 2012-08-15 | 2014-02-19 | 邱世连 | Novel milk replacing feed for suckling pigs |
| CN106819383A (en) * | 2017-01-19 | 2017-06-13 | 陆川县三农畜牧服务有限公司 | It is a kind of to promote fermented feed of piglet growth and preparation method thereof |
| CN109007439A (en) * | 2018-07-25 | 2018-12-18 | 广州格雷特生物科技有限公司 | Containing callicarpa nudiflora prawn feed additive and its preparation method and application |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08173055A (en) * | 1994-12-21 | 1996-07-09 | Shinichi Hoshida | Mannose-based polysaccharide-containing feed |
-
2002
- 2002-06-11 CN CNB021120579A patent/CN1317968C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100403921C (en) * | 2005-06-06 | 2008-07-23 | 北京新纪元三色生态科技有限公司 | A feed addictive, its preparation process and use |
| CN102178059A (en) * | 2011-05-13 | 2011-09-14 | 青岛诺和诺康生物技术有限公司 | Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof |
| CN102178059B (en) * | 2011-05-13 | 2013-01-02 | 青岛诺和诺康生物技术有限公司 | Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof |
| CN103583840A (en) * | 2012-08-15 | 2014-02-19 | 邱世连 | Novel milk replacing feed for suckling pigs |
| CN106819383A (en) * | 2017-01-19 | 2017-06-13 | 陆川县三农畜牧服务有限公司 | It is a kind of to promote fermented feed of piglet growth and preparation method thereof |
| CN109007439A (en) * | 2018-07-25 | 2018-12-18 | 广州格雷特生物科技有限公司 | Containing callicarpa nudiflora prawn feed additive and its preparation method and application |
| CN109007439B (en) * | 2018-07-25 | 2021-12-14 | 广州格雷特生物科技有限公司 | Prawn feed additive containing callicarpa nudiflora and preparation method and application thereof |
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| Publication number | Publication date |
|---|---|
| CN1317968C (en) | 2007-05-30 |
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