CN114304407A - Feeding probiotic additive for ruminants and application thereof - Google Patents
Feeding probiotic additive for ruminants and application thereof Download PDFInfo
- Publication number
- CN114304407A CN114304407A CN202210024329.6A CN202210024329A CN114304407A CN 114304407 A CN114304407 A CN 114304407A CN 202210024329 A CN202210024329 A CN 202210024329A CN 114304407 A CN114304407 A CN 114304407A
- Authority
- CN
- China
- Prior art keywords
- sweet potato
- additive
- feed
- ruminants
- probiotic additive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 41
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 41
- 239000000654 additive Substances 0.000 title claims abstract description 38
- 230000000996 additive effect Effects 0.000 title claims abstract description 38
- 241000282849 Ruminantia Species 0.000 title claims abstract description 30
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 30
- 244000017020 Ipomoea batatas Species 0.000 claims abstract description 76
- 235000002678 Ipomoea batatas Nutrition 0.000 claims abstract description 76
- 238000000855 fermentation Methods 0.000 claims abstract description 41
- 230000004151 fermentation Effects 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 239000002351 wastewater Substances 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 20
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 18
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 18
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 18
- 238000001035 drying Methods 0.000 claims abstract description 9
- 230000012010 growth Effects 0.000 claims abstract description 6
- 230000036039 immunity Effects 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 8
- 238000009630 liquid culture Methods 0.000 claims description 7
- 229920000858 Cyclodextrin Polymers 0.000 claims description 6
- 239000001116 FEMA 4028 Substances 0.000 claims description 6
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 6
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 6
- 229960004853 betadex Drugs 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000003223 protective agent Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 230000002708 enhancing effect Effects 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 229920001202 Inulin Polymers 0.000 claims description 2
- 229940029339 inulin Drugs 0.000 claims description 2
- 229940032147 starch Drugs 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 14
- 239000002699 waste material Substances 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 238000009700 powder processing Methods 0.000 abstract description 10
- 239000000126 substance Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 8
- 241000894006 Bacteria Species 0.000 abstract description 7
- 241000283690 Bos taurus Species 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 230000000968 intestinal effect Effects 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 244000144972 livestock Species 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 230000031891 intestinal absorption Effects 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract description 2
- 244000144977 poultry Species 0.000 abstract 2
- 235000019621 digestibility Nutrition 0.000 abstract 1
- 238000005265 energy consumption Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 12
- 244000005700 microbiome Species 0.000 description 11
- 235000015097 nutrients Nutrition 0.000 description 10
- 210000004767 rumen Anatomy 0.000 description 10
- 239000002609 medium Substances 0.000 description 9
- 235000016709 nutrition Nutrition 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 241000186660 Lactobacillus Species 0.000 description 5
- 244000309466 calf Species 0.000 description 5
- 230000001079 digestive effect Effects 0.000 description 5
- 229940039696 lactobacillus Drugs 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 235000015278 beef Nutrition 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000033458 reproduction Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000002366 mineral element Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 229920001592 potato starch Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- XJRPTMORGOIMMI-UHFFFAOYSA-N ethyl 2-amino-4-(trifluoromethyl)-1,3-thiazole-5-carboxylate Chemical compound CCOC(=O)C=1SC(N)=NC=1C(F)(F)F XJRPTMORGOIMMI-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- OQTQHQORDRKHFW-UHFFFAOYSA-L manganese(2+);sulfate;heptahydrate Chemical compound O.O.O.O.O.O.O.[Mn+2].[O-]S([O-])(=O)=O OQTQHQORDRKHFW-UHFFFAOYSA-L 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a feeding probiotic additive for ruminants and application thereof. The additive is prepared by fermenting lactobacillus acidophilus in a fermentation medium of waste water of sweet potato residue and sweet potato powder, and drying the fermentation liquid to directly serve as the additive. The feed probiotics additive is used for feeding cattle, and the number of the viable bacteria of the lactobacillus acidophilus in the feed probiotics additive reaches 8.9 x 109cfu/g. Compared with other feed probiotics, the method has the advantages of low raw material cost, high production efficiency, convenience in use, short production period, low energy consumption and the like, and can be used for preparing the feed probiotics for calvesThe feed is used for raising cattle, so that the feed digestibility of cattle is improved, the feed is more beneficial to the intestinal absorption of livestock and poultry, the intestinal environment of the livestock and poultry is improved, the immunity of the animals is enhanced, the obvious effect of promoting the growth of the animals is achieved, and meanwhile, the production wastes such as the sweet potato residue and the sweet potato powder processing wastewater are changed into valuable substances, so that the recycling of waste resources is realized.
Description
Technical Field
The invention belongs to the technical field of preparation of feeding probiotics, and particularly relates to a feeding probiotic additive for ruminants and application thereof.
Background
The probiotics can mainly maintain organisms with intestinal microbial balance, as beneficial microorganisms, widely exist in nature, are widely regarded and applied as veterinary drugs and feed additives, beneficially influence host animals by improving the intestinal microbial balance of the host animals, can be used for replacing feed antibiotics, enhance immunity, promote health and growth of the animals, improve feeding efficiency, reduce feeding cost, and can be used for replacing the feed antibiotics, reducing antibiotic residues of animal products and inhibiting drug-resistant pathogenic bacteria. The sweet potato powder is an important food material in common diet, a large amount of sweet potato residues and processing wastewater can be generated in the production and processing processes, the direct discharge utilization rate is low, and waste of waste resources and environmental pollution can be caused. Since these two by-products contain a large amount of carbon source substances such as starch and glucose, it is considered to utilize both of them as resources.
The acidophilic lactobacillus belongs to the family of lactobacillus, is rod-shaped, has extremely strong acid resistance, can generate lactic acid and acetic acid and generate antibiotics to inhibit harmful bacteria, prevent the propagation of harmful microorganisms, can enhance the immunity of organisms, can realize mass planting in animal intestinal tracts, helps digestion and metabolism of food, and promotes the absorption and utilization of nutrient substances; meanwhile, the feed additive can compete with pathogenic bacteria, inhibit the reproduction of harmful bacteria, improve the environment in intestinal tracts, adjust the balance of gastrointestinal flora and improve the health level of animals.
The digestive physiology of ruminants is quite different from that of monogastric mammals and is much more complex than that of monogastric mammals. The digestion of three nutrients, carbohydrates, lipids and proteins, by monogastric mammals is achieved by decomposing them into simpler forms by the digestive juices and then absorbing them, and then using them to maintain the energy required for their physiological activities or to synthesize various substances required for their metabolism, growth, development and reproduction. In monogastric animals, sugars are usually broken down by digestive juices into monosaccharides (glucose, fructose, galactose, mannose, etc.) and disaccharides (maltose, sucrose and lactose), which are absorbed and then oxidized in the body to produce energy for metabolic activities or synthesized into fat stores; the fat is obtained by decomposing higher lipids into triglyceride or other lower lipids under the action of digestive juice, and absorbing the triglyceride or other lower lipids to serve as an energy source or synthesize autologous fat again for storage; the protein is hydrolyzed into amino acids under the action of digestive juice, and the amino acids are used as components for synthesizing self-protein again. In ruminants, they do not directly digest and absorb nutrients in the feed by themselves, and either type of nutrient is first fermented by rumen microorganisms, and organic acids and some primary fatty acids, which are finally produced during the fermentation process, can be absorbed by the rumen as energy sources for their metabolism, while other nutrients are derived from the digestion of rumen microorganisms.
In animal production, the acidophilic lactobacillus can achieve the effects of increasing daily gain, improving the material-weight ratio, enhancing the disease resistance of organisms, increasing the survival rate, reducing the diarrhea rate, inhibiting harmful bacteria and the like, effectively ensure the edible safety of livestock products, and simultaneously improve the economic benefit. In the current report, a precedent that the sweet potato residue and the sweet potato powder processing wastewater are mixed together and fermented by probiotics to produce the feed probiotics does not appear. The method has the advantages of effectively reducing the cost and realizing resource utilization by adopting the fermentation of the crude raw materials, along with wide market prospect.
Disclosure of Invention
The invention aims to provide a feeding probiotic additive for ruminants and application thereof, sweet potato residue and sweet potato powder processing wastewater are mixed together for fermentation of probiotics for the first time, and the obtained fermentation product contains a large amount of probiotic thalli and active nutrient substances, so that the nutrition for rumen microorganism propagation of the ruminants and the requirement for mineral elements are fully met, the rumen microorganisms are propagated to the maximum extent, the ruminants can obtain more thalli nutrition, and the conversion rate of feed is improved.
In order to achieve the purpose, the invention adopts the technical scheme that:
a feed probiotic additive for ruminants is prepared by fermenting Lactobacillus acidophilus in a fermentation medium of waste water of sweet potato residue and sweet potato powder, and drying the fermentation liquid to obtain the feed probiotic additive.
A preparation method of a feeding probiotic additive for ruminants comprises the following steps:
s1, sweet potato residue pretreatment: cleaning sweet potato residue, removing impurities, and drying at 70-90 deg.C until water content is 20-30%: sealing the dried sweet potato residue in a closed container, and carrying out damp-heat treatment for 2-6h at the temperature of 115-130 ℃; drying the sweet potato residue subjected to the damp-heat treatment at the temperature of 70-90 ℃ for 2-4h, crushing, and sieving with a 80-100-mesh sieve to obtain modified sweet potato residue powder;
s2, pretreatment of sweet potato powder wastewater: filtering the sweet potato powder wastewater for later use;
s3, inoculating lactobacillus acidophilus to a fermentation medium after plate culture and liquid seed liquid culture, and fermenting at the temperature of 28-45 ℃ for 24-72h to obtain liquid fermentation liquid;
s4, adding 15-40% of protective agent into the liquid fermentation liquor, mixing, and spray-drying at the air inlet temperature of 250-350 ℃ and the air outlet temperature of 80-120 ℃ to obtain the feed probiotic additive.
The fermentation medium consists of sweet potato residues and sweet potato powder wastewater, the content of the sweet potato residues is 20-80 g/L, and the balance is the sweet potato powder wastewater. The content of the sweet potato residues is preferably 40-60 g/L, and the content of the sweet potato residues is further preferably 50 g/L.
The inoculation proportion in S3 is 2% -8%. Further preferably, the inoculation ratio is 5%.
The protective agent in S4 is at least one of starch, maltodextrin, beta-cyclodextrin or inulin. Further preferably, the protective agent is beta-cyclodextrin.
In S1, the wet heat treatment is preferably performed at a temperature of 120 ℃ for 3 hours.
The colony number in the feed probiotic additive is more than or equal to 7.8 x 109cfu/g。
The feed probiotic additive for the ruminants is applied to enhancing the immunity of the ruminants and promoting the growth of the ruminants.
The additive is added into ruminant feed, and the addition amount of the additive is 100-400 g per kilogram of feed.
The lactobacillus acidophilus is a common strain in the prior art and can be obtained from a commercial way.
The waste water from processing the sweet potato residue and the sweet potato powder is waste generated in the production process of sweet potatoes, and the processing method comprises the steps of carrying out damp-heat treatment on the sweet potato residue and then physically crushing the sweet potato residue to ensure that the particles of the sweet potato residue and the sweet potato powder are uniform; the waste water of the sweet potato residue and the sweet potato residue after being treated and crushed are respectively used as fermentation raw materials, waste of waste resources is reduced, meanwhile, because the waste water of the sweet potato residue and the sweet potato processing contains a large amount of carbon source substances such as starch, glucose and the like and various mineral elements, the waste water is fermented by probiotics, and the obtained fermentation product contains a large amount of probiotics thallus and active nutrient substances and is added into feed, so that rumen microorganisms can be proliferated to the maximum extent, and ruminants can obtain more thallus nutrition. The proliferation of rumen microorganism can convert more non-protein nitrogen into mycoprotein which can be used by cattle and sheep, and has important significance for improving the utilization rate of ruminant feed and enhancing the nutritional level. Minerals have a dual role in ruminant nutrition, and are essential not only for maintaining normal metabolism of the animal, but also for the reproduction of rumen microorganisms. Fully meets the requirement of rumen microorganisms on minerals, and plays an important role in improving the conversion rate of the feed.
The method for detecting the number of the live bacteria in the microbial inoculum is a flat plate counting method.
Advantageous effects
The invention directly adopts the sweet potato residue and the sweet potato powder processing wastewater as the culture medium for the strain fermentation, does not need to add other nutrient substances, greatly saves the production cost, realizes the reutilization of wastes, changes wastes into valuables, fully utilizes the special mineral substances in the sweet potato residue and sweet potato powder processing wastewater, has high thallus concentration in the fermentation liquid obtained by fermentation, also contains various active nutrient substances beneficial to rumen microorganisms, and has the highest total viable count of 8.9 to 10 in the product9The cfu/mL is beneficial to intestinal absorption of ruminants, improves the intestinal environment, enhances the immunity of animals, has the obvious effect of promoting the growth of the animals, changes waste into valuable in production, realizes the recycling of waste resources, reduces the pollution of waste materials to the environment, greatly reduces the production cost, is economical and feasible, has low raw material cost, high production efficiency, convenient use, short production period and high nutritional value, and has wide market application value.
Detailed Description
The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the specific process conditions and results described in the examples are merely illustrative of the invention and should not, nor should they, limit the invention as described in detail in the claims.
The strains referred to in the following examples are: lactobacillus acidophilus is purchased from Biotechnology Ltd, the family Chloraceae, Jiangsu.
The waste water from processing sweet potato residue and sweet potato flour described in the following examples was purchased from sweet potato flour processing and producing companies. The processing method of the processed sweet potato residue described in the following examples is as follows: cleaning sweet potato residue, removing impurities, and drying at 890 ℃ until the water content is 25%: sealing the dried sweet potato residue in a closed container, and carrying out damp-heat treatment for 3h at the temperature of 120 ℃; drying the sweet potato residue subjected to the damp-heat treatment at the temperature of 80 ℃ for 3 hours, crushing, and sieving with a 80-100-mesh sieve to obtain modified sweet potato residue powder;
filtering the sweet potato powder processing wastewater to remove suspended matters, and then mixing the filtered sweet potato powder processing wastewater with the processed sweet potato residue powder to be used as a fermentation medium.
The method for liquid fermentation of lactobacillus acidophilus comprises the following steps,
(1) solid and liquid culture medium for strain
Lactobacillus acidophilus solid medium: 10.0 g of peptone, 3.0 g of beef extract (beef extract), 5.0 g of lactose, 5.0 g of sodium chloride and 0.016 g of bromocresol purple, wherein the volume is up to 1000ml, and the pH value is 6.2-6.4. The culture time is 18-24 hours; the solid culture medium is liquid culture medium added with 15-20% agar.
Lactobacillus acidophilus liquid culture medium: 10.0 g of peptone, 10.0 g of beef extract (beef extract), 5.0 g of yeast extract (yeast extract or yeast powder), 20.0 g of glucose, 5.0 g of sodium acetate, 2.0 g of diammonium hydrogen citrate, 801.0 ml of tween-801.0 ml, 2.0 g of dipotassium hydrogen phosphate, 0.2 g of magnesium sulfate heptahydrate and 0.05 g of manganese sulfate heptahydrate, and the volume is fixed to 1000ml, and the pH value is 6.2-6.4.
The sterilization method comprises the following steps: sterilizing with high pressure steam at 115 deg.C for 15min for saccharides, and 121 deg.C for 20min for the rest.
(2) Solid plate culture of strain
Taking out a glycerol tube of lactobacillus acidophilus from a refrigerator at the temperature of minus 80 ℃, sucking 100 microliters of bacteria liquid by using a 100ml liquid transfer gun in a super clean bench, coating the bacteria liquid on a solid culture medium plate, putting the lactobacillus acidophilus in a bacterial incubator at the temperature of 37 ℃ for culturing for 24 hours, carrying out four-zone lineation after the culture is finished, and transferring a single colony which appears after the lactobacillus acidophilus is placed in the incubator at the temperature of 37 ℃ for culturing for 24 hours to a solid culture medium for carrying out dense lineation.
(3) Liquid seed culture of strain
Inoculating Lactobacillus acidophilus into 500ml shake flask containing 200ml culture medium, placing into 37 deg.C incubator, standing for culturing for 24-36 h.
Inoculating the lactobacillus liquid strain into a fermentation culture medium according to the ratio of 2-8% (W/W), and fermenting at 28-45 ℃ for 24-72h to obtain liquid fermentation liquor.
Adding 15-40% of beta-cyclodextrin into the liquid fermentation liquor for mixing, and spraying the mixture to dry at the air inlet temperature of 250-350 ℃ and the air outlet temperature of 80-120 ℃ to obtain the feed probiotic additive, wherein the colony count is more than or equal to 7.8 x 109cfu/g。
Example 1:
in the embodiment, the inoculation ratio of lactobacillus acidophilus is considered, and fermentation experiments are carried out in culture media inoculated into the sweet potato residue and sweet potato powder processing wastewater according to different (W/W) inoculation amounts, wherein the fermentation time is 24-72 h. The inoculation ratios were 2%, 4%, 5%, 6%, 8%, 10%, respectively, and the total colony number of the fermentation product was determined, and the results are shown in Table 1. As shown in Table 1, the optimum seed liquid ratio was 5%.
TABLE 1 Effect of different bacterial species ratios on colony counts
Example 2
According to the invention, the fermentation time is considered, the liquid seed liquid with the lactobacillus acidophilus inoculation proportion of 5 percent is inoculated into the liquid culture medium of the sweet potato powder processing wastewater and the sweet potato residue, the fermentation temperature is 37 ℃, and the following table 2 is shown.
TABLE 2 Effect of different fermentation times on colony counts
Example 3
The fermented liquid is added with different auxiliary materials for spray drying, the addition amount of the auxiliary materials is 30%, and the colony count is obviously different, as shown in Table 3 below.
TABLE 3 Effect of different adjuvants on colony counts
Auxiliary materials | Total colony count, cfu/g |
Starch | 3.4×109 |
Maltodextrin | 4.5×109 |
Beta-cyclodextrin | 8.9×109 |
Inulin powder | 4.8×108 |
Example 4
According to the invention, the sweet potato residue content in the fermentation medium is considered, the sweet potato residue content in the medium of the culture machine is respectively 10g/L, 20g/L, 30g/L, 40g/L, 50g/L and 60g/L, the liquid seed liquid with the lactobacillus acidophilus inoculation proportion of 5% is inoculated into the liquid culture medium of the sweet potato powder processing wastewater and the sweet potato residue, the fermentation temperature is 37 ℃, and the following table 4 shows.
TABLE 4 influence of different sweet potato residue ratios on the number of colonies
Example 5
Fermentation medium: the content of the sweet potato residue is 30g/L, and the balance is the waste water of the sweet potato powder.
Inoculating lactobacillus liquid strain 5% (W/W) into fermentation medium, and fermenting at 37 deg.C for 48 hr to obtain liquid fermentation broth.
Adding 30% of beta-cyclodextrin into the liquid fermentation liquor, mixing, and spray-drying at air inlet temperature of 280 ℃ and air outlet temperature of 100 ℃ to obtain the feed probiotic additive with colony count of 8.9 multiplied by 109cfu/g, and also contains a large amount of active nutrients.
Example 6
The invention researches the feeding probiotic additive in the calf feeding. The feed probiotic additive of example 5 was added to calf feed, 200g of the probiotic additive was added per kg of the feed, and the test was carried out using the fermented feed of the present invention as a test group, using a normal feed without the probiotic additive as a blank control group. The calves were fed freely for 1 month with the results shown in Table 5.
TABLE 5 Effect of animal feed of the invention on Calf
As can be seen from table 5: compared with a blank control group, the daily gain of calves fed with the feed probiotic additive is increased by 10.42%, the feed conversion ratio is reduced by 15.51%, the diarrhea rate is reduced by 75.00%, and the survival rate is increased by 8.89%.
The technical solutions provided by the embodiments of the present invention are described in detail above, and the principles and implementations of the embodiments of the present invention are described herein by using specific examples, and the descriptions of the above embodiments are only used to help understand the principles of the embodiments of the present invention, and for those skilled in the art, the details and implementations of the embodiments and implementations may be changed, and in summary, the content of the present description should not be understood as a limitation to the present invention.
Claims (10)
1. A feeding probiotic additive for ruminants, characterized in that: the additive is prepared by fermenting lactobacillus acidophilus in a fermentation medium of waste water of sweet potato residue and sweet potato powder, and drying the fermentation liquid to directly serve as the additive.
2. A preparation method of a feeding probiotic additive for ruminants is characterized by comprising the following steps:
s1, sweet potato residue pretreatment: cleaning sweet potato residue, removing impurities, and drying at 70-90 deg.C until water content is 20-30%: sealing the dried sweet potato residue in a closed container, and carrying out damp-heat treatment for 2-6h at the temperature of 115-130 ℃; drying the sweet potato residue subjected to the damp-heat treatment at the temperature of 70-90 ℃ for 2-4h, crushing, and sieving with a 80-100-mesh sieve to obtain modified sweet potato residue powder;
s2, pretreatment of sweet potato powder wastewater: filtering the sweet potato powder wastewater for later use;
s3, inoculating lactobacillus acidophilus to a fermentation medium after plate culture and liquid seed liquid culture, and fermenting at the temperature of 28-45 ℃ for 24-72h to obtain liquid fermentation liquid;
s4, adding 15-40% of protective agent into the liquid fermentation liquor, mixing, and spray-drying at the air inlet temperature of 250-350 ℃ and the air outlet temperature of 80-120 ℃ to obtain the feed probiotic additive.
3. The method of preparing a feeding probiotic additive for ruminants according to claim 2, wherein the method comprises the following steps: the fermentation medium consists of sweet potato residues and sweet potato powder wastewater, the content of the sweet potato residues is 20-80 g/L, and the balance is the sweet potato powder wastewater.
4. The method of preparing a feeding probiotic additive for ruminants according to claim 2, wherein the method comprises the following steps: the inoculation proportion in the S3 is 2% -8%.
5. The method of preparing a feeding probiotic additive for ruminants according to claim 2, wherein the method comprises the following steps: the protective agent in S4 is at least one of starch, maltodextrin, beta-cyclodextrin or inulin.
6. The method of preparing a feeding probiotic additive for ruminants according to claim 2, wherein the method comprises the following steps: in S1, the wet heat treatment is preferably performed at a temperature of 120 ℃ for 3 hours.
7. The method of preparing a feeding probiotic additive for ruminants according to claim 3, wherein the method comprises the following steps: the content of the sweet potato residue in the fermentation medium is 30-60 g/L.
8. The method of preparing a feeding probiotic additive for ruminants according to claim 3, wherein the method comprises the following steps: the colony number in the feed probiotic additive is more than or equal to 7.8 x 109cfu/g。
9. The use of a feed probiotic additive for ruminants as claimed in claim 1 for enhancing the immunity and growth of ruminants.
10. The use of claim 9, wherein the additive is added to ruminant feed in an amount of 100-400 g per kg feed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210024329.6A CN114304407A (en) | 2022-01-10 | 2022-01-10 | Feeding probiotic additive for ruminants and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210024329.6A CN114304407A (en) | 2022-01-10 | 2022-01-10 | Feeding probiotic additive for ruminants and application thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN114304407A true CN114304407A (en) | 2022-04-12 |
Family
ID=81026732
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210024329.6A Pending CN114304407A (en) | 2022-01-10 | 2022-01-10 | Feeding probiotic additive for ruminants and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114304407A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115804423A (en) * | 2022-12-23 | 2023-03-17 | 山西农业大学 | Feed additive for cashmere goats and preparation method thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104855686A (en) * | 2015-04-24 | 2015-08-26 | 中国科学院亚热带农业生态研究所 | A protein feed produced by fermenting sweet potato residues and a method to prolong shelf life thereof |
CN105941860A (en) * | 2016-06-17 | 2016-09-21 | 湖北省农业科学院农产品加工与核农技术研究所 | Sweet potato byproduct feed and microbial fermentation method thereof |
CN105941839A (en) * | 2016-05-04 | 2016-09-21 | 北京科技大学 | Method for preparing probiotic feed by using by-products generated in production process of vermicelli |
-
2022
- 2022-01-10 CN CN202210024329.6A patent/CN114304407A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104855686A (en) * | 2015-04-24 | 2015-08-26 | 中国科学院亚热带农业生态研究所 | A protein feed produced by fermenting sweet potato residues and a method to prolong shelf life thereof |
CN105941839A (en) * | 2016-05-04 | 2016-09-21 | 北京科技大学 | Method for preparing probiotic feed by using by-products generated in production process of vermicelli |
CN105941860A (en) * | 2016-06-17 | 2016-09-21 | 湖北省农业科学院农产品加工与核农技术研究所 | Sweet potato byproduct feed and microbial fermentation method thereof |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115804423A (en) * | 2022-12-23 | 2023-03-17 | 山西农业大学 | Feed additive for cashmere goats and preparation method thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107603924B (en) | Compound microbial preparation and preparation method and application thereof | |
CN106260540B (en) | Biological feed for creep feed and creep feed | |
CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
CN102008016B (en) | Probiotic solid powder and preparation method thereof | |
CN104017751B (en) | Bacillus coagulans, Lactobacillus plantarum and Lactobacillus casei preparation and its preparation | |
CN103734549B (en) | Large-grain milk replacer and preparing method thereof | |
CN104371960B (en) | Composite fungus agent and the continuous fermentation method of complex microorganism adopted | |
CN107312732B (en) | Probiotic feed additive | |
CN109757605B (en) | Method for producing animal feed by fermenting corn alcohol lees and waste bean dregs | |
CN113215051A (en) | Method for preparing feed probiotics by using lactobacillus through rice flour wastewater and passion fruit peel | |
CN110810625A (en) | Waste mushroom stick biological fermentation feed and preparation method thereof | |
CN107788332B (en) | Probiotic high-efficiency toxin adsorbent and production method and application thereof | |
CN106035990B (en) | Method for preparing biological feed by solid-state enzymolysis fermentation of citrus pulp, product and application thereof | |
CN113508872B (en) | Palm meal raw material biological pretreatment method | |
CN113773996A (en) | Preparation method and application of feeding bacillus subtilis | |
CN114304379A (en) | Preparation method of fermented feed containing compound microbial agent | |
CN114304407A (en) | Feeding probiotic additive for ruminants and application thereof | |
CN111466480B (en) | Tea residue and tea polysaccharide probiotic fermented feed and preparation method thereof | |
CN1500400A (en) | Enzyme-enriched prebiotics and its preparation | |
CN112342256A (en) | Method for preparing feed-grade fructo-oligosaccharide by taking molasses as raw material | |
CN105211637A (en) | Livestock biological feed nourishing additive agent and production method thereof | |
CN110558420A (en) | High-dietary-fiber low-antigen-protein fermented soybean hull | |
CN107041456B (en) | Fermented bean dreg feed additive and preparation method and application thereof | |
CN111728081B (en) | Composite bacteria fermentation liquor for feed additive and preparation method thereof | |
CN114231434A (en) | Clostridium butyricum for feed additive and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |