CN102178059B - Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof - Google Patents
Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof Download PDFInfo
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- CN102178059B CN102178059B CN2011101232229A CN201110123222A CN102178059B CN 102178059 B CN102178059 B CN 102178059B CN 2011101232229 A CN2011101232229 A CN 2011101232229A CN 201110123222 A CN201110123222 A CN 201110123222A CN 102178059 B CN102178059 B CN 102178059B
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Abstract
The invention provides a microcological feed additive for lowering the concentration of ammonia in a livestock and poultry house and application of the microcological feed additive. The microcological feed additive comprises the following components: bacillus subtilis in the ratio of 3.92-4.08*10<9>pcs/g, bacillus licheniformis in the ratio of 0.98-1.02*10<9>pcs/g, butyrate clostridium in the ratio of 1.96-2.04*10<8>pcs/g, SSF (sodium fluosilicate) enzyme prepared by aspergillus oryzae in the ratio of 148-152mg/g and a carrier in the ratio of 98-102mg/g. The microcological feed additive for lowering the concentration of ammonia in the livestock and poultry house can improve protein absorbing capacity of an intestinal canal of livestock and poultry through producing enzyme, thus a nitrogensource is reduced; growth of putrefying bacteria is inhibited by beneficial bacteria, thus emission of the ammonia is reduced; and the activity of urease is inhibited, and the ammonia generated by uric acid and urea under the catalysis of urease is reduced, thus the aim of lowering the concentration of the ammonia in a livestock house is achieved.
Description
Technical field
The invention belongs to the livestock feed technical field, relate to microbial forage additive, be specifically related to a kind of microbial forage additive and application thereof that reduces the poultry house ammonia concentration.
Background technology
Raising livestock and poultry can provide the necessitys such as meat, egg and milk of daily need to people; have extremely important social agency and economic function; but its feeding also causes people's attention day by day on the impact of poultry house external environment; its feeding tends to exhaust emission gas in the poultry house external environment; be considered to one of the largest source of greenhouse gas emission; therefore cause very large environmental pollution, the dusty gas of its feeding discharging also is put into the important content of whole world environmental protection.
At present, along with the fast development of animal husbandry, extensive, sealing, intensive feeding manner constantly increase.Although this has greatly improved production efficiency and the economic benefit of animal husbandry, but, its feeding has not only sharply increased the weight of the to external world pollution of environment, and cause harmful gas in livestock house constantly to increase, it is high to have a strong impact on health and the production performance of animal body, particularly the breathing problem incidence of disease.According to statistics, there is 86.5% poultry-farm to cause performance of poultry decline owing to having infected the respiratory tract disease in the feeding process in the middle of the failed poultry-farm of cultivation.
Therefore, the pernicious gas that reduces in the poultry house just becomes urgent problem, pernicious gas in the poultry house mainly is ammonia, the at present measure of minimizing poultry house ammonia concentration has a variety of, for example, increases the animal house height, antibacterial ammonia system, the ammonium sulfate recovery system etc. of pressing down of little electric current are installed, but these measure investments are excessive, energy consumption is too much, and animal is used in the match that generally is used for high added value, and perhaps the grandfather is for kind of livestock and poultry.Because the method cost of reduction ammonia concentration commonly used at present is too high, there is defective in itself, can't realize commercial application.
Summary of the invention
The present invention is directed in the prior art deficiencies such as the existing high cost of method, the consumed energy that reduce ammonia concentration in the poultry house be too much, the present invention is according to the generation principle of ammonia in the poultry house, the composition of feed and the situation of feeding environment, a kind of microbial forage additive and application thereof that reduces the poultry house ammonia concentration is provided, feed addictive of the present invention can add in daily ration and drinking-water, the mode of action is various, the in vivo external equal discharging that can reduce ammonia is the optimal selection that cultivation site reduces ammonia emission.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
A kind of microbial forage additive that reduces the poultry house ammonia concentration is characterized in that: described microbial forage additive comprises following component:
Bacillus subtilis 3.92 * 10
9Individual/gram-4.08 * 10
9Individual/gram;
Bacillus licheniformis 0.98 * 10
9Individual/gram-1.02 * 10
9Individual/gram;
Clostridium butyricum 1.96 * 10
8Individual/gram-2.04 * 10
8Individual/gram;
Aspergillus oryzae prepares 148 milligrams of SSF enzymes/-152 milligrams/gram of gram;
-102 milligrams/gram of 98 milligrams/gram of carrier.
Further improvement to technical scheme: the bacillus subtilis bacterial content is preferably 4.0 * 10 in the described microbial forage additive
9Individual/gram, bacillus licheniformis content is preferably 1.0 * 10
9Individual/gram, clostridium butyricum content is preferably 2.0 * 10
8Individual/gram, the content that aspergillus oryzae prepares the SSF enzyme is preferably 150 milligrams/gram, and vector contg is preferably 100 milligrams/gram.
Further improvement to technical scheme: described carrier is one or more in maize peel powder, wheat bran or the powdered rice hulls, and 30 order oversizes are 0.
The present invention also provides described microbial forage additive in the application that reduces on the poultry house ammonia concentration.
Further improvement to technical scheme: the addition of described microbial forage additive is according to 1 ‰ of perfect compound feed gross weight-2 ‰, directly this product is added in the livestock and poultry basal diet perfect compound feed, or be mixed and made into premix with other feed addictive or feedstuff, the concentrate feed form is fed to the livestock and poultry of intensive farm.
Compared with prior art, advantage of the present invention and good effect are: the present invention utilizes the principle that the microbial forage additive energy is antibacterial, suppress urease-producing, uricase is active and suppresses to produce enzyme, the microbial forage additive that can reduce the poultry house ammonia concentration is provided, it can reduce the breeding of harmful bacteria in the animal and bird intestines, be conducive to reduce the too much generation of corrupt substance, thereby reduce the foul smell of ight soil; It can reduce the generation of ammonia; Bacillus licheniformis in the described feed addictive and bacillus subtilis can breed in enteron aisle and produce a large amount of 1,4 beta-glucanases, zytase, а-galactosidase etc., the enzyme that produces can significantly improve the energy utilization of daily ration, thereby reduces the above emission intensity amount of 10 %.
The specific embodiment
Below in conjunction with the specific embodiment technical scheme of the present invention is described in further detail.
The preparation of embodiment 1, microbial forage additive of the present invention
Described microbial forage additive refers to promote beneficial microbe and the metabolite thereof of microbial ecological balance in the animal body.
The concrete preparation method of microbial forage additive of the present invention is as follows: get 350 kilograms of the spray-dired bacillus subtilis solids of liquor fermentation, 350 kilograms of the spray-dired bacillus licheniformis of liquor fermentation, 50 kilograms of the clostridium butyricums of liquor fermentation press filtration oven dry, 150 kilograms of mixing of SSF enzyme of 120 ℃ of oven dry of solid fermentation, add again 100 kilograms in 40 purpose maize peel powder and insert in the mixer 200 rev/mins and mixed 10 minutes, namely make 1 ton of finished product.Carrier also can be selected wheat bran or powdered rice hulls.Above-mentioned bacillus subtilis, bacillus licheniformis, clostridium butyricum, SSF enzyme and carrier all can obtain by commercial purchase approach.
The dominant mechanism that microbial forage additive of the present invention reduces ammonia concentration is as follows:
Antibacterial: as can to cause the amount of protein transduction corrupt substance to increase during the movable enhancing of the harmful bacterias such as Escherichia coli, thereby the generation of ammonia is increased.And can reduce the breeding of harmful bacteria when beneficial bacterium increases in the enteron aisle, and help to reduce the too much generation of corrupt substance, reduce the foul smell of ight soil.Can suppress the growth of putrefactivebacteria behind the animal absorption microbial forage additive, add microbial forage additive in the Broiler Dietary and can significantly suppress colibacillary breeding, the amount that reduces the excrement ammonia is 30.14 %.
Suppress urease-producing, uricase activity: reduce the generation of ammonia by the microbial activity that suppresses urease-producing and uricase.The bacillus subtilis flora can utilize the materials such as ammonia, hydrogen sulfide to be nutrition when growth and breeding, thereby reduces the discharge of ammonia.
Produce enzyme: commonly used have bacillus licheniformis and a bacillus subtilis, and these two kinds of bacterium can sprout field planting 72h at enteron aisle, and breeding 3-4 produces a large amount of 1,4 beta-glucanases, zytase, а-galactosidase etc. more than generation during this period; 1,4 beta-glucanase can improve 11.9 % with amino acid digestibility in broiler chicken ileum Corn-soybean, the Wheat Based Diet; Zytase can improve the meat chick to nitrogen use efficiency 7% in the Corn-soybean daily ration; Galactosidase can improve dregs of beans rate of ultilization of amino acid 5 %～10 %, significantly improves meat chick Corn-soybean daily ration energy utilization 5%; Capacity interpolation micro-ecological bacterial group can reduce more than 10 % of emission intensity aspect the product enzyme in the daily ration.
Embodiment 2, microbial forage additive of the present invention are on the impact of pouity dwelling place ammonia concentration
Choose 8 hen houses (hen house size: 120m * 14m/, the cultivation amount: 16500 plumages /), be divided into immediately two groups, test group (adding this product 1000g/T in the basal diet) and control group (basal diet).Air sampling: apart from ground 30cm; Sample point: respectively get 3 calculating mean values after in before the hen house; Sample time: the morning 7:00, unit: ppm.
The described microbial forage additive of table 1 is on the impact of pouity dwelling place ammonia concentration
| Age in days | Control group | Test group | P |
| 7 ages in days | 10.17±2.13 | 10.83±2.08 | 0.795 |
| 14 ages in days | 29.94±5.93 | 25.27±5.68 | 0.678 |
| 21 ages in days | 47.33±10.94 | 38.59±9.42 | 0.236 |
| 28 ages in days | 58.25±13.61 | 41.17±14.53 | 0.049 |
As shown in Table 1, from 7 ages in days to 28 ages in days, along with the increase of chicken age in days, the generation of ammonia constantly increases.But test group is because search for food and contain the daily ration of microbial forage additive, and the concentration of pouity dwelling place ammonia decreases than control group, and along with the increase of daily ration, this trend is all the more obvious, and when 28 age in days, P＜0.05 reaches the level of signifiance.
Embodiment 3, microbial forage additive of the present invention are on the impact of pig house ammonia concentration
Choose 4 120-150 age in days growing and fattening pigs pig houses (pig house size: 45m * 10m; Cultivation amount: 50 * 4 hurdles), be divided at random two groups, test group (adding this product 1000g/T in the basal diet) and control group (basal diet).Air sampling: apart from ground 60cm.Sample point: respectively get 3 calculating mean values after in before the pig house.Sample time: the morning 7:00, unit: ppm.
The described microbial forage additive of table 2 is on the impact of pig house ammonia concentration
| Age in days | Control group | Test group | P |
| Before feeding | 57.86±7.43 | 57.75±6.48 | 0.864 |
| Fed 3 days | 58.49±8.16 | 34.64±5.29 | 0.742 |
| Fed 7 days | 49.05±10.94 | 39.17±7.42 | 0.132 |
| Fed 15 days | 62.15±12.61 | 40.03±8.46 | 0.021 |
As shown in Table 2, before feeding, the pig house ammonia concentration of test group and control group is basic identical, feeds rear 3 days to 15 days, compares test group pig house ammonia concentration downward trend increasingly significant with control group.
Above embodiment is only in order to illustrating technical scheme of the present invention, but not limits it; Although with reference to previous embodiment the present invention is had been described in detail, for the person of ordinary skill of the art, still can make amendment to the technical scheme that previous embodiment is put down in writing, perhaps part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the scope of the present invention's technical scheme required for protection.
Claims (5)
1. microbial forage additive that reduces the poultry house ammonia concentration, it is characterized in that: described microbial forage additive comprises following component:
Bacillus subtilis 3.92 * 10
9Individual/gram-4.08 * 10
9Individual/gram;
Bacillus licheniformis 0.98 * 10
9Individual/gram-1.02 * 10
9Individual/gram;
Clostridium butyricum 1.96 * 10
8Individual/gram-2.04 * 10
8Individual/gram;
Aspergillus oryzae prepares 148 milligrams of SSF enzymes/-152 milligrams/gram of gram;
-102 milligrams/gram of 98 milligrams/gram of carrier.
2. a kind of microbial forage additive that reduces the poultry house ammonia concentration according to claim 1, it is characterized in that: the bacillus subtilis bacterial content is preferably 4.0 * 10 in the described microbial forage additive
9Individual/gram, bacillus licheniformis content is preferably 1.0 * 10
9Individual/gram, clostridium butyricum content is preferably 2.0 * 10
8Individual/gram, the content that aspergillus oryzae prepares the SSF enzyme is preferably 150 milligrams/gram, and vector contg is preferably 100 milligrams/gram.
3. a kind of microbial forage additive that reduces the poultry house ammonia concentration according to claim 1 and 2, it is characterized in that: described carrier is one or more in maize peel powder, wheat bran or the powdered rice hulls, 30 order oversizes are 0.
4. microbial forage additive according to claim 1 is in the application that reduces on the poultry house ammonia concentration.
5. a kind of application that reduces the microbial forage additive of poultry house ammonia concentration according to claim 4, it is characterized in that: the addition of described microbial forage additive is according to 1 ‰ of perfect compound feed gross weight-2 ‰, directly this product is added in the livestock and poultry basal diet perfect compound feed, or be mixed and made into premix with other feed addictive or feedstuff, the concentrate feed form is fed to the livestock and poultry of intensive farm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101232229A CN102178059B (en) | 2011-05-13 | 2011-05-13 | Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof |
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| CN2011101232229A CN102178059B (en) | 2011-05-13 | 2011-05-13 | Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof |
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| CN102178059B true CN102178059B (en) | 2013-01-02 |
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Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102910791A (en) * | 2012-10-16 | 2013-02-06 | 青岛根源生物技术集团有限公司 | Micro ecological additive and application thereof in reducing ammonia emission |
| CN104186971A (en) * | 2014-08-01 | 2014-12-10 | 湖南丹维生物科技有限公司 | Anti-stress agent for piglet and preparation method of anti-stress agent |
| CN105248897A (en) * | 2015-09-11 | 2016-01-20 | 四川特驱投资集团有限公司 | Pig forage improving meat quality |
| CN105053724A (en) * | 2015-09-11 | 2015-11-18 | 四川特驱投资集团有限公司 | Fodder for growing and fattening pigs |
| CN105994955A (en) * | 2016-05-19 | 2016-10-12 | 山东信得科技股份有限公司 | Compound deodorant special for livestock and poultry and preparation method and application thereof |
| CN106819366A (en) * | 2017-01-21 | 2017-06-13 | 潍坊中创药业有限公司 | One kind removes the absorbefacient feed addictive of ammonia and its application |
| CN109007273A (en) * | 2018-06-06 | 2018-12-18 | 广东谷盈生物科技产业研究院有限公司 | A kind of multi-cultur es composite microbial feed additive and its application |
| CN109198194A (en) * | 2018-10-31 | 2019-01-15 | 西北农林科技大学 | The feed addictive and preparation method and application of pernicious gas content in a kind of reduction animal house |
| CN110699299B (en) * | 2019-11-14 | 2023-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof |
| CN110747126A (en) * | 2019-11-14 | 2020-02-04 | 河北农业大学 | Bacterial strain screening method for producing urease inhibitor and method for reducing excrement ammonia emission |
| CN110643550B (en) * | 2019-11-14 | 2022-12-06 | 河北农业大学 | Bacillus subtilis J530 strain for producing urease inhibitor and application thereof |
| CN111826323A (en) * | 2020-08-05 | 2020-10-27 | 福建洛东生物技术有限公司 | Bacillus subtilis, preparation and application thereof |
| CN113278560A (en) * | 2021-06-15 | 2021-08-20 | 无锡拜弗德生物科技有限公司 | Microecological preparation for improving pet odor |
| CN115316538A (en) * | 2022-09-01 | 2022-11-11 | 上海市农业科学院 | Fermented preparation and feed suitable for piglets |
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