CN1434873B - 选择性分离核酸的方法 - Google Patents

选择性分离核酸的方法 Download PDF

Info

Publication number
CN1434873B
CN1434873B CN008183082A CN00818308A CN1434873B CN 1434873 B CN1434873 B CN 1434873B CN 008183082 A CN008183082 A CN 008183082A CN 00818308 A CN00818308 A CN 00818308A CN 1434873 B CN1434873 B CN 1434873B
Authority
CN
China
Prior art keywords
nucleic acid
target
population
acid molecule
target element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN008183082A
Other languages
English (en)
Chinese (zh)
Other versions
CN1434873A (zh
Inventor
约翰尼斯·戴普赖奇
米歇尔·A·克莱厄尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Princeton University
Original Assignee
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University filed Critical Princeton University
Publication of CN1434873A publication Critical patent/CN1434873A/zh
Application granted granted Critical
Publication of CN1434873B publication Critical patent/CN1434873B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN008183082A 1999-12-10 2000-12-11 选择性分离核酸的方法 Expired - Fee Related CN1434873B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US17014099P 1999-12-10 1999-12-10
US60/170,140 1999-12-10
US73384600A 2000-12-08 2000-12-08
US09/733,846 2000-12-08
PCT/US2000/033579 WO2001042510A2 (en) 1999-12-10 2000-12-11 Method for selectively isolating a nucleic acid

Publications (2)

Publication Number Publication Date
CN1434873A CN1434873A (zh) 2003-08-06
CN1434873B true CN1434873B (zh) 2012-11-14

Family

ID=26865748

Family Applications (1)

Application Number Title Priority Date Filing Date
CN008183082A Expired - Fee Related CN1434873B (zh) 1999-12-10 2000-12-11 选择性分离核酸的方法

Country Status (8)

Country Link
EP (1) EP1240359B1 (enExample)
JP (1) JP4731081B2 (enExample)
CN (1) CN1434873B (enExample)
AT (1) ATE414787T1 (enExample)
AU (1) AU785211B2 (enExample)
CA (1) CA2393874C (enExample)
DE (1) DE60040864D1 (enExample)
WO (1) WO2001042510A2 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844154B2 (en) 2000-04-04 2005-01-18 Polygenyx, Inc. High throughput methods for haplotyping
EP1784508B1 (en) * 2004-08-09 2012-10-03 Generation Biotech, LLC Method for nucleic acid isolation and amplification
MX364309B (es) * 2013-04-17 2019-04-22 Pioneer Hi Bred Int Métodos para caracterizar la composición de secuencias de adn en un genoma.

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1127887A (zh) * 1994-09-07 1996-07-31 株式会社日立制作所 Dna分离、分级分离和分析方法及其系统

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665582A (en) * 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
JPH08507198A (ja) * 1992-07-02 1996-08-06 エリフィーレ ビー．フェ． 特異的対立遺伝子の検出のための単一ヌクレオチドプライマー伸張法およびそのためのキット
AU3519900A (en) * 1999-03-19 2000-10-09 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
JP2004511210A (ja) * 2000-05-23 2004-04-15 バリアジェニックス　インコーポレーテッド 配列変動を検出する、ｄｎａを遺伝分析するための方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1127887A (zh) * 1994-09-07 1996-07-31 株式会社日立制作所 Dna分离、分级分离和分析方法及其系统

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
实施例1，3
说明书2页9行－10页6行
附图1－2。.

Also Published As

Publication number Publication date
WO2001042510A2 (en) 2001-06-14
EP1240359B1 (en) 2008-11-19
AU785211B2 (en) 2006-11-02
EP1240359A2 (en) 2002-09-18
CA2393874A1 (en) 2001-06-14
AU2086701A (en) 2001-06-18
WO2001042510A3 (en) 2002-07-11
CN1434873A (zh) 2003-08-06
JP2004500062A (ja) 2004-01-08
JP4731081B2 (ja) 2011-07-20
CA2393874C (en) 2011-02-08
DE60040864D1 (de) 2009-01-02
ATE414787T1 (de) 2008-12-15

Similar Documents

Publication Publication Date Title
US20080090733A1 (en) Method for selectively isolating a nucleic acid
JP5249581B2 (ja) 核酸の単離および増幅方法
US5571676A (en) Method for mismatch-directed in vitro DNA sequencing
AU697642B2 (en) High throughput screening method for sequences or genetic alterations in nucleic acids
JP4498657B2 (ja) Ｄｎａサンプル中のシトシン−メチル化とｓｎｐ又は突然変異の同時検出方法
EP0850315B1 (en) Detection of mismatches by resolvase cleavage using a magnetic bead support
US20080274904A1 (en) Method of target enrichment
US20070141604A1 (en) Method of target enrichment
WO1997009444A9 (en) Detection of mismatches by resolvase cleavage using a magnetic bead support
JP2010063469A (ja) ヌクレオチドの変異について核酸をスクリーニングする方法
JP2003521252A (ja) ユニバーサルプライミングを用いる核酸検出方法
JP2004520812A (ja) 対立遺伝子を決定するための方法
WO1996035809A1 (en) Detection of mismatches by resolvase cleavage on a solid support
JP2001517936A (ja) 選択的連結及び増幅方法
JPH11506937A (ja) Ｄｎａの配列決定およびポジショナルクローニングを含む、ｄｎａの遺伝子的修飾を特定する方法
CN1434873B (zh) 选择性分离核酸的方法
US20030082549A1 (en) Method for determining alleles
JP2003505036A (ja) 核酸決定のための多重鎖置換
WO2025078657A1 (en) Amplification-free target enrichment workflow for direct detection of nucleic acid modifications
WO2001032929A1 (en) Methods and compositions for analysis of snps and strs
WO2001046470A1 (en) Enrichment of nucleic acid
JP2002034599A (ja) １塩基多型を検出する方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121114

Termination date: 20131211