CN1377225A - 使用来自分化细胞的供体细胞或细胞核克隆猪以及多能猪的生成 - Google Patents

使用来自分化细胞的供体细胞或细胞核克隆猪以及多能猪的生成 Download PDF

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Publication number
CN1377225A
CN1377225A CN00813714A CN00813714A CN1377225A CN 1377225 A CN1377225 A CN 1377225A CN 00813714 A CN00813714 A CN 00813714A CN 00813714 A CN00813714 A CN 00813714A CN 1377225 A CN1377225 A CN 1377225A
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China
Prior art keywords
cell
pig
nucleus
unit
embryo
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Pending
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CN00813714A
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English (en)
Chinese (zh)
Inventor
S·L·斯蒂斯
J·赛贝利
J·罗贝尔
P·格鲁克
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University of Massachusetts Amherst
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University of Massachusetts Amherst
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Publication date
Priority claimed from US09/394,902 external-priority patent/US7291764B1/en
Application filed by University of Massachusetts Amherst filed Critical University of Massachusetts Amherst
Publication of CN1377225A publication Critical patent/CN1377225A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN00813714A 1999-09-13 2000-09-13 使用来自分化细胞的供体细胞或细胞核克隆猪以及多能猪的生成 Pending CN1377225A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/394,902 1999-09-13
US09/394,902 US7291764B1 (en) 1997-01-10 1999-09-13 Cloning pigs using non-quiescent differentiated donor cells or nuclei

Publications (1)

Publication Number Publication Date
CN1377225A true CN1377225A (zh) 2002-10-30

Family

ID=23560867

Family Applications (1)

Application Number Title Priority Date Filing Date
CN00813714A Pending CN1377225A (zh) 1999-09-13 2000-09-13 使用来自分化细胞的供体细胞或细胞核克隆猪以及多能猪的生成

Country Status (9)

Country Link
EP (1) EP1241931A4 (enExample)
JP (1) JP2003509031A (enExample)
CN (1) CN1377225A (enExample)
AU (1) AU7372500A (enExample)
BR (1) BR0013959A (enExample)
CA (1) CA2381124A1 (enExample)
IL (1) IL148508A0 (enExample)
MX (1) MXPA02002653A (enExample)
WO (1) WO2001019181A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100404675C (zh) * 2006-02-22 2008-07-23 李宁 一种生产体细胞克隆猪的方法
CN111349615A (zh) * 2018-12-24 2020-06-30 上海细胞治疗集团有限公司 制备过表达外源基因的细胞的方法
CN115305260A (zh) * 2021-11-30 2022-11-08 海南大学 一种金鲳鱼受精卵的显微注射方法及应用
CN116640804A (zh) * 2023-04-20 2023-08-25 云南农业大学 一种基于体细胞核移植技术构建三倍体猪的方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004275074A (ja) * 2003-03-14 2004-10-07 Nipro Corp 動物胚における正常性のスクリーニング方法
WO2008106984A2 (en) * 2007-03-07 2008-09-12 Aarhus Universitet Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex
AU2015360502A1 (en) 2014-12-10 2017-06-29 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease
WO2016140353A1 (ja) * 2015-03-04 2016-09-09 株式会社 医療実験用大動物供給事業準備会社 安定した表現型を示す疾患モデルブタおよびその作製方法
KR20230110373A (ko) * 2016-06-14 2023-07-21 리전츠 오브 더 유니버스티 오브 미네소타 질환을 치료하기 위한 유전적으로 변형된 세포, 조직,및 장기
CN117044679B (zh) * 2023-10-13 2024-01-19 成都铁骑力士饲料有限公司 一种血清fas在川藏黑猪s05系瘦肉型猪选育中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0739412B1 (en) * 1993-12-23 2002-02-27 Infigen, Inc. ungulate EMBRYONIC STEM CELLS AS NUCLEAR DONORS AND NUCLEAR TRANSFER TECHNIQUES TO PRODUCE CHIMERIC AND TRANSGENIC ANIMALS
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100404675C (zh) * 2006-02-22 2008-07-23 李宁 一种生产体细胞克隆猪的方法
CN111349615A (zh) * 2018-12-24 2020-06-30 上海细胞治疗集团有限公司 制备过表达外源基因的细胞的方法
CN115305260A (zh) * 2021-11-30 2022-11-08 海南大学 一种金鲳鱼受精卵的显微注射方法及应用
CN115305260B (zh) * 2021-11-30 2023-09-22 海南大学 一种金鲳鱼受精卵的显微注射方法及应用
CN116640804A (zh) * 2023-04-20 2023-08-25 云南农业大学 一种基于体细胞核移植技术构建三倍体猪的方法

Also Published As

Publication number Publication date
BR0013959A (pt) 2002-05-21
AU7372500A (en) 2001-04-17
WO2001019181A1 (en) 2001-03-22
EP1241931A4 (en) 2004-12-29
CA2381124A1 (en) 2000-09-13
EP1241931A1 (en) 2002-09-25
IL148508A0 (en) 2002-09-12
MXPA02002653A (es) 2003-10-14
JP2003509031A (ja) 2003-03-11

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