BR0013959A - Clonagem de porcos usando células ou núcleos doadores a partir de células diferenciadas e produção de porcino pluripotente - Google Patents

Clonagem de porcos usando células ou núcleos doadores a partir de células diferenciadas e produção de porcino pluripotente

Info

Publication number
BR0013959A
BR0013959A BR0013959-9A BR0013959A BR0013959A BR 0013959 A BR0013959 A BR 0013959A BR 0013959 A BR0013959 A BR 0013959A BR 0013959 A BR0013959 A BR 0013959A
Authority
BR
Brazil
Prior art keywords
production
cells
donor
differentiated
nuclei
Prior art date
Application number
BR0013959-9A
Other languages
English (en)
Inventor
Steven L Stice
Jose Cibelli
James Robl
Paul Golueke
Original Assignee
Univ Massachusetts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/394,902 external-priority patent/US7291764B1/en
Application filed by Univ Massachusetts filed Critical Univ Massachusetts
Publication of BR0013959A publication Critical patent/BR0013959A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Patente de Invenção: "CLONAGEM DE PORCOS USANDO CéLULAS OU NúCLEOS DOADORES A PARTIR DE CéLULAS DIFERENCIADAS E PRODUçãO DE PORCINO PLURIPOTENTE". é proporcionado um método aperfeiçoado de transferência nuclear envolvendo a transplantação de núcleos de células de porcos diferenciadas doadores em oócitos de porcas enucleados. As unidades de transferência nuclear resultantes são úteis para a multiplicação de genótipos e de genótipos transgênicos pela produção de fetos e proles. A produção de embriões, fetos e proles de porcos geneticamente engenheirados ou transgênicos é facilitada pelo presente método, visto que a fonte de células diferenciadas dos núcleos doadores pode ser geneticamente modificada e propagada de forma cional.
BR0013959-9A 1999-09-13 2000-09-13 Clonagem de porcos usando células ou núcleos doadores a partir de células diferenciadas e produção de porcino pluripotente BR0013959A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/394,902 US7291764B1 (en) 1997-01-10 1999-09-13 Cloning pigs using non-quiescent differentiated donor cells or nuclei
PCT/US2000/024958 WO2001019181A1 (en) 1999-09-13 2000-09-13 Cloning pigs using donor cells or nuclei from differentiated cells and production of pluripotent porcine.

Publications (1)

Publication Number Publication Date
BR0013959A true BR0013959A (pt) 2002-05-21

Family

ID=23560867

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0013959-9A BR0013959A (pt) 1999-09-13 2000-09-13 Clonagem de porcos usando células ou núcleos doadores a partir de células diferenciadas e produção de porcino pluripotente

Country Status (9)

Country Link
EP (1) EP1241931A4 (pt)
JP (1) JP2003509031A (pt)
CN (1) CN1377225A (pt)
AU (1) AU7372500A (pt)
BR (1) BR0013959A (pt)
CA (1) CA2381124A1 (pt)
IL (1) IL148508A0 (pt)
MX (1) MXPA02002653A (pt)
WO (1) WO2001019181A1 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004275074A (ja) * 2003-03-14 2004-10-07 Nipro Corp 動物胚における正常性のスクリーニング方法
CN100404675C (zh) * 2006-02-22 2008-07-23 李宁 一种生产体细胞克隆猪的方法
CA2717251A1 (en) * 2007-03-07 2008-09-12 Aarhus Universitet Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex
CN116059378A (zh) 2014-12-10 2023-05-05 明尼苏达大学董事会 用于治疗疾病的遗传修饰的细胞、组织和器官
WO2016140353A1 (ja) * 2015-03-04 2016-09-09 株式会社 医療実験用大動物供給事業準備会社 安定した表現型を示す疾患モデルブタおよびその作製方法
KR20190017985A (ko) * 2016-06-14 2019-02-20 리전츠 오브 더 유니버스티 오브 미네소타 질환을 치료하기 위한 유전적으로 변형된 세포, 조직, 및 장기
CN111349615B (zh) * 2018-12-24 2024-08-13 上海细胞治疗集团股份有限公司 制备过表达外源基因的细胞的方法
CN115305260B (zh) * 2021-11-30 2023-09-22 海南大学 一种金鲳鱼受精卵的显微注射方法及应用
CN116640804A (zh) * 2023-04-20 2023-08-25 云南农业大学 一种基于体细胞核移植技术构建三倍体猪的方法
CN117044679B (zh) * 2023-10-13 2024-01-19 成都铁骑力士饲料有限公司 一种血清fas在川藏黑猪s05系瘦肉型猪选育中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1149898A2 (en) * 1993-12-23 2001-10-31 Infigen, Inc. Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells

Also Published As

Publication number Publication date
IL148508A0 (en) 2002-09-12
CA2381124A1 (en) 2000-09-13
EP1241931A4 (en) 2004-12-29
MXPA02002653A (es) 2003-10-14
EP1241931A1 (en) 2002-09-25
CN1377225A (zh) 2002-10-30
AU7372500A (en) 2001-04-17
JP2003509031A (ja) 2003-03-11
WO2001019181A1 (en) 2001-03-22

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A ANUIDADE.

B08H Application fees: decision cancelled [chapter 8.8 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1849 DE 13/06/2006 POR TER SIDO APRESENTADA PAPELETA DE ESCLARECIMENTO.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]