CN1375492A - Method of extracting soybean isoflavone - Google Patents
Method of extracting soybean isoflavone Download PDFInfo
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- CN1375492A CN1375492A CN02114991A CN02114991A CN1375492A CN 1375492 A CN1375492 A CN 1375492A CN 02114991 A CN02114991 A CN 02114991A CN 02114991 A CN02114991 A CN 02114991A CN 1375492 A CN1375492 A CN 1375492A
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- soybean
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- cotyledon
- plumular axis
- isoflavone
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Abstract
The extraction method of soybean isoflavone includes the following steps: soaking ripe and plump soybean seed in cold water to pullulation state, peeling, seed leaf splitting, separating seed leaf from soybean embryonal axis, adopting solvent leaching-out process to obtain said invented soybean isoflavone, the separated soybean seed leaf also can be used as industrial raw material for deep-processing soybean. Said soybean isoflavone can be used for improving the diseases of hot flush and vaginitis of menopausal woman, and possesses the protection action for cardiovessel and has obvious effect for preventing cancers.
Description
Technical field
The present invention relates to a kind of by the method for soybean seeds sprouting period separation soybean plumular axis for extracting soybean isoflavone.
Background technology
Soybean isoflavones (Isoflavones of Soybean) is mainly derived from fabaceous pod beans, wherein the content in the soybean is higher, be distributed in soybean seeds plumular axis and the cotyledon, content is few in the kind skin, isoflavone content is 0.1%～0 3% in the cotyledon, and isoflavone content is 1%～2% in the plumular axis.Soybean isoflavones is divided into the glucoside unit (Aglycon) of free type and glucoside (Glucosides) two classes of mating type, glucoside unit accounts for the 2%-3% of total amount, comprise Sophoricol (Gen) and big legumin (Dai) and soya bean glucoside (Gly): glucoside accounts for the 97%-98% of total amount, mainly with genistin (Genistin Genistin) and Daidzin (Daidzin) and 6''-O-malonylgenistin (6 " 0-malonylgenistin) and malonyl-Daidzin (6 "-0-malonyldacidzin) form existence.Human experimentation shows that soybean isoflavones mainly is absorbed in enteron aisle, specific absorption is 10%～40%, and the soybean of bud can strengthen the absorption of soybean isoflavones after edible, and specific absorption is 20%～60%.Discover, the estrogen-like effects of soybean isoflavones has influence on sex hormone secretion, metabolism and biologic activity, protein synthesis, growth factor activity, cell malignant proliferation, differentiation, cell adhesion and vasculogenesis, its effect to important steroid biosynthesizing key enzyme also influences the hormone in vivo level, help to improve symptoms such as postmenopausal women's hot flush and vaginitis, the generation of osteoporosis is also had prophylactic effect.Therefore, by soybean deep processing comprehensive utilization, separating soybean plumular axis from soybean seeds is a kind of economically viable method for extracting soybean isoflavone as medical material or functional health product.
Summary of the invention
The purpose of this invention is to provide a kind of by the method for soybean seeds sprouting period separation soybean plumular axis for extracting soybean isoflavone.
Cover because the special morphological structure of soybean seeds, the soybean plumular axis of sesame size are close to the cotyledon of two hypertrophys and are formed cornified fine and close palisade tissue kind suitcase, it is very difficult to separate complete soybean plumular axis usually.The present invention finds the soybean seeds cotyledon and the soybean plumular axis energy delamination of the initial bud of soybean seeds sprouting period and keeps the complete and contained soybean isoflavones biological activity of plumular axis not to be damaged.
Method of the present invention is that soybean seeds is sprouted to initial bud through cold water soak, through decortication, soybean cotyledon distinguish, cotyledon separate with soybean plumular axis, technique process such as solvent extraction method extraction, obtain soybean isoflavones.Present method can effectively keep contained soybean isoflavones biological activity and not be damaged.
The concrete steps of the inventive method are as follows:
One, cold water soak is sprouted to initial bud: with the cold water soak soybean seeds to being initial bud; Water temperature is 10～34 ℃, is generally 17～26 ℃; Soak time is 4 hours to 18 hours, is generally 6～14 hours.
The soybean seeds sprouting period is from water-swelling, and along with the increase of plumular axis and cotyledon water content, protoplasma is transformed into collosol state by gel state, and respiratory metabolism strengthens, and plants skin and expands, and oxygen penetrates, and the enzyme system activity strengthens.Paotoblastic metabolism promotes the plumular axis elongation to be initial bud.
Used soybean seeds is fresh with mature and plump, complete, the no disease and pest of shape and kind skin light to be good.Preferably reject flat grain, broken lobe grain and the grain of damaging by worms through screening.
Two, decortication operation: the soybean seeds that will be initial bud places common industrial dejacketer stirring at low speed to roll and peeled 1 minute to 3 minutes, rotating speed is 30 rev/mins to 500 rev/mins, the soybean seeds cotyledon that is the expansion bud is peeled off easily with planting skin, further placed separator tank to add the soybean peel that water will be suspended in the water surface and separate with cotyledon.
Three, soybean cotyledon distinguish and cotyledon separate with soybean plumular axis: the soybean cotyledon that will peel places common industrial dejacketer, adopt middling speed to stir scroll process 1 minute to 5 minutes, rotating speed is 600 rev/mins to 5000 rev/mins, make two cotyledons that are the state of holding tightly by distinguish, and the soybean plumular axis of being close to soybean cotyledon is separated to be peeled off, and further places separator tank to add the soybean plumular axis that water will be half suspended state and separates with the soybean cotyledon of the distinguish that is deposited in bottom land.The soybean cotyledon of distinguish can be used as raw material and is applied to the soybean deep processing industries after warm air drying.
Four, extracting soybean isoflavone: the solvent extraction extraction glucoside that the polarity that the soybean plumular axis of separating is adopted usually with industrial production is stronger, further adopt the more weak solvent extraction extraction glucoside unit of polarity again; Combining extraction liquid and be total soybean isoflavones after cryodrying.Leaching extraction can adopt industry leaching plant commonly used, can select flat-turn leacher for use, ring-like leacher, crawler belt-frame leacher or pot type leacher etc.; Mixed solution separates can adopt the mixed solution stripping apparatus, can select type equipment such as disk stripping column, laminar stripping tower, material filling type stripping tower or tubular type stripping tower for use.Can adopt following processing condition during leaching extraction: 20～100 ℃ of extraction temperatures, extraction temperature is 40～55 ℃ usually; Solvent and plumular axis weight ratio are 0.2～3: 1; Extraction time is 3～180 minutes, is generally 60～80 minutes.In the soybean plumular axis leaching extraction process, used solvent can be recycled.
Soybean isoflavones is divided into the glucoside unit (Aglycon) of free type and glucoside (Glucosides) two classes of mating type, and therefore, the extraction of total soybean isoflavones can adopt two step of industrial production lixiviation process to extract.Available ethyl acetate, acetone, ethanol, methyl alcohol, water or the stronger mixed solvent of some polarity as glucoside extract as methyl alcohol and water (1: 1); Glucoside unit is with the more weak organic solvent of polarity, as extractions such as ether, chloroform, ethanol ethyl esters.
The inventive method adopts cold water soak that soybean seeds is sprouted to initial bud, and soybean plumular axis is easily separated with cotyledon, and method is simply effective, soybean isoflavones percentage extraction height, and can effectively keep contained soybean isoflavones biological activity and be not damaged.The soybean cotyledon of being separated also can be used as soybean deep processing industries raw material, helps making full use of of soybean resource.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
100 kilograms of the soybean seeds of screening mature and plump place cold water to soak 9.5 hours, and soaking water temperature is 21 ℃, and soybean seeds has expanded and has been initial bud; Peeled off soybean peel in 1 minute through industrial dejacketer stirring at low speed rolling decortication, rotating speed is 40 rev/mins, placing separator tank to add the kind skin that water will be suspended in the water surface separates with soybean cotyledon, stirring to roll through industrial dejacketer middling speed again made two cotyledons that are the state of hugging by distinguish in 2.5 minutes, soybean plumular axis is separated to be peeled off, rotating speed is 3000 rev/mins, and the soybean plumular axis that places separator tank will be half suspended state once more separates with soybean cotyledon.The soybean plumular axis of separating is positioned over leaching extraction in the flat-turn leacher through two step of industrial production lixiviation process, press plumular axis and solvent ethyl acetate weight ratio extraction in 1: 1 glucoside, extraction temperature is 50 ℃, extraction time is 70 minutes, mixed solution separates employing tubular type stripping tower, and then extract glucoside unit by plumular axis and 0.8: 1 weight ratio of solvent ether of being extracted behind the glucoside, extraction temperature is 55 ℃, extraction time is 50 minutes, mixed solution separates employing tubular type stripping tower, and combining extraction liquid is total soybean isoflavones after cryodrying.
Embodiment two:
100 kilograms of the soybean seeds of screening mature and plump place cold water to soak 10 hours, and soaking water temperature is 20 ℃, and soybean seeds has expanded and has been initial bud; Peeled off soybean peel in 3 minutes through industrial dejacketer stirring at low speed rolling decortication, rotating speed is 30 rev/mins, placing separator tank to add the kind skin that water will be suspended in the water surface separates with soybean cotyledon, stirring to roll through industrial dejacketer middling speed again made two cotyledons that are the state of hugging by distinguish in 5 minutes, soybean plumular axis is separated to be peeled off, rotating speed is 600 rev/mins, and the soybean plumular axis that places separator tank will be half suspended state once more separates with soybean cotyledon.Be positioned over leaching extraction in the ring-like leacher through two step of industrial production lixiviation process, solvent methanol and water (1: 1) 2: 1 weight ratio stronger by plumular axis and polarity extract glucoside, extraction temperature is 42 ℃, extraction time is 10 minutes, mixed solution separates employing dish formula stripping tower, and then extract glucoside unit by plumular axis and 0.2: 1 weight ratio of solvent chloroform of being extracted behind the glucoside, extraction temperature is 45 ℃, extraction time is 10 minutes, mixed solution separates employing dish formula stripping tower, and combining extraction liquid is total soybean isoflavones after cryodrying.
Embodiment three:
100 kilograms of the soybean seeds of screening mature and plump place cold water to soak 13 hours, and soaking water temperature is 17 ℃, and soybean seeds has expanded and has been initial bud; Peeled off soybean peel in 2 minutes through industrial dejacketer stirring at low speed rolling decortication, rotating speed is 200 rev/mins, placing separator tank to add the kind skin that water will be suspended in the water surface separates with soybean cotyledon, adopting middling speed to stir rolling through industrial dejacketer again made two cotyledons that are the state of hugging by distinguish in 4 minutes, soybean plumular axis is separated to be peeled off, rotating speed is 1000 rev/mins, and the soybean plumular axis that places separator tank will be half suspended state once more separates with soybean cotyledon.Be positioned over leaching extraction in the track type leacher through two step of industrial production lixiviation process, press plumular axis and etoh solvent weight ratio extraction in 0.5: 1 glucoside, extraction temperature is 48 ℃, extraction time is 80 minutes, and mixed solution separate to adopt the laminar stripping tower, and then extracts glucoside unit by plumular axis and 1.8: 1 weight ratios of solvent ether of being extracted behind the glucoside, extraction temperature is 49 ℃, extraction time is 20 minutes, and mixed solution separates employing laminar stripping tower, and combining extraction liquid is total soybean isoflavones after cryodrying.
Embodiment four:
100 kilograms of the soybean seeds of screening mature and plump place cold water to soak 7.5 hours, and soaking water temperature is 26 ℃, and soybean seeds has expanded and has been initial bud; Peeled off soybean peel in 1.5 minutes through industrial dejacketer stirring at low speed rolling decortication, rotating speed is 300 rev/mins, placing separator tank to add the kind skin that water will be suspended in the water surface separates with soybean cotyledon, adopting middling speed to stir rolling through industrial dejacketer again made two cotyledons that are the state of hugging by distinguish in 3.5 minutes, soybean plumular axis is separated to be peeled off, rotating speed is 1500 rev/mins, and the soybean plumular axis that places separator tank will be half suspended state once more separates with soybean cotyledon.Be positioned over leaching extraction in crawler belt-frame leacher through two step of industrial production lixiviation process, press plumular axis and solvent acetone weight ratio extraction in 0.3: 1 glucoside, extraction temperature is 80 ℃, extraction time is 80 minutes, mixed solution separates employing material filling type stripping tower, and then extract glucoside unit by plumular axis and 0.8: 1 weight ratio of etoh solvent ethyl ester of being extracted behind the glucoside, extraction temperature is 38 ℃, extraction time is 50 minutes, mixed solution separates employing material filling type stripping tower, and combining extraction liquid is total soybean isoflavones after cryodrying.
Embodiment five:
100 kilograms of the soybean seeds of screening mature and plump place cold water to soak 10 hours, and soaking water temperature is 22 ℃, and soybean seeds has expanded and has been initial bud; Peel off soybean peel 50 seconds through the decortication of rolling of industrial dejacketer stirring at low speed, rotating speed is 450 rev/mins, placing separator tank to add the kind skin that water will be suspended in the water surface separates with soybean cotyledon, adopting middling speed to stir rolling through industrial dejacketer again made two cotyledons that are the state of hugging by distinguish in 2 minutes, soybean plumular axis is separated to be peeled off, rotating speed is 3000 rev/mins, and the soybean plumular axis that places separator tank will be half suspended state once more separates with soybean cotyledon.Be positioned over leaching extraction in the pot type leacher through two step of industrial production lixiviation process, press plumular axis and solvent methanol weight ratio extraction in 0.5: 1 glucoside, extraction temperature is 20 ℃, extraction time is 180 minutes, and mixed solution separate to adopt dish formula stripping tower, and then extracts glucoside unit by plumular axis and 0.8: 1 weight ratio of solvent chloroform of being extracted behind the glucoside, extraction temperature is 70 ℃, extraction time is 12 minutes, and mixed solution separates employing dish formula stripping tower, and combining extraction liquid is total soybean isoflavones after cryodrying.
Embodiment six:
100 kilograms of the soybean seeds of screening mature and plump place cold water to soak 18 hours, and soaking water temperature is 10 ℃, and soybean seeds has expanded and has been initial bud; Peeled off soybean peel in 1 minute through industrial dejacketer stirring at low speed rolling decortication, rotating speed is 300 rev/mins, placing separator tank to add the kind skin that water will be suspended in the water surface separates with soybean cotyledon, adopting middling speed to stir rolling through industrial dejacketer again made two cotyledons that are the state of hugging by distinguish in 1.5 minutes, soybean plumular axis is separated to be peeled off, rotating speed is 4000 rev/mins, and the soybean plumular axis that places separator tank will be half suspended state once more separates with soybean cotyledon.Be positioned over leaching extraction in crawler belt-frame leacher through two step of industrial production lixiviation process, press plumular axis and solvent acetone weight ratio extraction in 0.7: 1 glucoside, extraction temperature is 50 ℃, extraction time is 80 minutes, mixed solution separates employing laminar stripping tower, and then extract glucoside unit by plumular axis and 2: 1 weight ratios of etoh solvent ethyl ester of being extracted behind the glucoside, extraction temperature is 80 ℃, extraction time is 5 minutes, mixed solution separates employing laminar stripping tower, and combining extraction liquid is total soybean isoflavones after cryodrying.
Embodiment seven:
100 kilograms of the soybean seeds of screening mature and plump place cold water to soak 4.5 hours, and soaking water temperature is 32 ℃, and soybean seeds has expanded and has been initial bud; Peeled off soybean peel in 1 minute through industrial dejacketer stirring at low speed rolling decortication, rotating speed is 300 rev/mins, adding the kind skin that water will be suspended in the water surface in the separator tank separates with soybean cotyledon, adopting middling speed to stir rolling through industrial dejacketer again made two cotyledons that are the state of hugging by distinguish in 1.5 minutes, soybean plumular axis is separated to be peeled off, rotating speed is 4000 rev/mins, and the soybean plumular axis that places separator tank will be half suspended state once more separates with soybean cotyledon.Be positioned over leaching extraction in crawler belt-frame leacher through two step of industrial production lixiviation process, press stronger mixed solvent methyl alcohol of plumular axis and polarity and water (1: 1) weight ratio extraction in 3: 1 glucoside, extraction temperature is 50 ℃, extraction time is 200 minutes, mixed solution separates employing material filling type stripping tower, and then by the plumular axis and more weak solvent such as ethanol ethyl ester weight ratio extraction in 3: the 1 glucoside unit of polarity that are extracted behind the glucoside, extraction temperature is 30 ℃, extraction time is 100 minutes, mixed solution separates employing material filling type stripping tower, and combining extraction liquid is total soybean isoflavones after cryodrying.
Claims (8)
1. the method for an extracting soybean isoflavone is characterized in that soybean seeds is sprouted to initial bud through cold water soak, separates with soybean plumular axis through decortication, soybean cotyledon distinguish, cotyledon, again soybean plumular axis is obtained soybean isoflavones through the extraction of solvent extraction method.
2. the method for an extracting soybean isoflavone as claimed in claim 1 is characterized in that concrete steps are:
(1), with the cold water soak soybean seeds to being initial bud; Water temperature is 10～34 ℃; Soak time is 4 hours to 18 hours;
(2), the soybean seeds that will be initial bud places the decortication 1 minute to 3 minutes of rolling of common industrial dejacketer stirring at low speed, rotating speed is 30 rev/mins to 500 rev/mins, the soybean seeds cotyledon that will be the expansion bud is peeled off with planting skin, further places separator tank to add the soybean peel that water will be suspended in the water surface and separates with cotyledon;
(3), the soybean cotyledon that will peel places common industrial dejacketer, adopt middling speed to stir scroll process 1 minute to 5 minutes, rotating speed is 600 rev/mins to 5000 rev/mins, two cotyledon distinguish that will be the state of holding tightly, and the soybean plumular axis that will be close to soybean cotyledon separates and to peel off, and further places separator tank to add the soybean plumular axis that water will be half suspended state and separates with the soybean cotyledon of the distinguish that is deposited in bottom land;
(4), the stronger solvent extraction extraction glucoside of polarity that the soybean plumular axis separated is adopted usually with industrial production, further adopt the more weak solvent extraction extraction glucoside unit of polarity again; Combining extraction liquid and be total soybean isoflavones after cryodrying.
3. the method for an extracting soybean isoflavone as claimed in claim 2 is characterized in that water temperature that the soaking soybean seed sprouts is for being 17～26 ℃.
4. the method for an extracting soybean isoflavone as claimed in claim 2 is characterized in that the soak time that the soaking soybean seed is sprouted is 6～12 hours.
The method of 5 one kinds of extracting soybean isoflavones as claimed in claim 2 is characterized in that the stronger solvent of the used polarity of described extracting soybean isoflavone is ethyl acetate, acetone, ethanol or methyl alcohol, or the mixed solvent of methyl alcohol and water; The more weak solvent of used polarity is ether, chloroform or ethanol ethyl ester.
6. method as the described extracting soybean isoflavone of one of claim 1 to 5, the concrete processing condition that it is characterized in that described leaching extraction glucoside and leaching extraction glucoside unit are: 20～100 ℃ of extraction temperatures, extraction time is 3～180 minutes, and solvent and plumular axis weight ratio are 0.2～3: 1.
7. the method for an extracting soybean isoflavone as claimed in claim 6 is characterized in that described extraction temperature is 40～55 ℃.
8. the method for an extracting soybean isoflavone as claimed in claim 6 is characterized in that described extraction time is 60～80 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021149917A CN1158273C (en) | 2002-03-22 | 2002-03-22 | Method of extracting soybean isoflavone |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021149917A CN1158273C (en) | 2002-03-22 | 2002-03-22 | Method of extracting soybean isoflavone |
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| CN1375492A true CN1375492A (en) | 2002-10-23 |
| CN1158273C CN1158273C (en) | 2004-07-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB021149917A Expired - Fee Related CN1158273C (en) | 2002-03-22 | 2002-03-22 | Method of extracting soybean isoflavone |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010004005A1 (en) * | 2008-07-10 | 2010-01-14 | Alliospharma | Compositions that aim to promote the development and growth of a beneficial vaginal microflora |
| CN105533210A (en) * | 2016-01-21 | 2016-05-04 | 成都华罗生物科技有限公司 | Medicine composition for improving piglet production performance and preparation method and application of medicine composition |
| CN105585546A (en) * | 2015-12-29 | 2016-05-18 | 成都华罗生物科技有限公司 | Soy isoflavone refining method |
| CN105614071A (en) * | 2015-12-28 | 2016-06-01 | 成都华罗生物科技有限公司 | Soybean isoflavone extract and extraction method thereof |
-
2002
- 2002-03-22 CN CNB021149917A patent/CN1158273C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010004005A1 (en) * | 2008-07-10 | 2010-01-14 | Alliospharma | Compositions that aim to promote the development and growth of a beneficial vaginal microflora |
| FR2933617A1 (en) * | 2008-07-10 | 2010-01-15 | Alliospharma | COMPOSITIONS FOR PROMOTING THE DEVELOPMENT AND GROWTH OF A BENEFICIAL VAGINAL MICROFLORE |
| CN105614071A (en) * | 2015-12-28 | 2016-06-01 | 成都华罗生物科技有限公司 | Soybean isoflavone extract and extraction method thereof |
| CN105585546A (en) * | 2015-12-29 | 2016-05-18 | 成都华罗生物科技有限公司 | Soy isoflavone refining method |
| CN105533210A (en) * | 2016-01-21 | 2016-05-04 | 成都华罗生物科技有限公司 | Medicine composition for improving piglet production performance and preparation method and application of medicine composition |
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| Publication number | Publication date |
|---|---|
| CN1158273C (en) | 2004-07-21 |
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