CN111187314A - Preparation method for extracting phlorizin from lithocarpus polystachyus rehd - Google Patents
Preparation method for extracting phlorizin from lithocarpus polystachyus rehd Download PDFInfo
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- CN111187314A CN111187314A CN201811355921.4A CN201811355921A CN111187314A CN 111187314 A CN111187314 A CN 111187314A CN 201811355921 A CN201811355921 A CN 201811355921A CN 111187314 A CN111187314 A CN 111187314A
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- phlorizin
- lithocarpus polystachyus
- rehd
- ethanol solution
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a preparation method for extracting phlorizin from lithocarpus polystachyus rehd, belonging to the technical field of processing. The preparation method is characterized by comprising the following preparation process flows: (1) selecting raw materials, cleaning, removing impurities, and pulverizing; (2) soaking the raw materials in 70-90% ethanol solution for 1-2 h; (3) ultrasonic-assisted extraction for 30-60min at 400-600 w/w at 30-80 deg.C for 2-3 times; (4) filtering to remove residues, adsorbing with macroporous resin column, and eluting with 50% ethanol solution; (5) recovering ethanol, concentrating, and drying to obtain Lithocarpus polystachyus (wall.) Rehd phlorizin. The method is simple and convenient to operate, easy for standardizing the process, easy for obtaining auxiliary materials for preparation and suitable for a factory production mode.
Description
Technical Field
The invention relates to a preparation method for extracting phlorizin from lithocarpus polystachyus rehd, belonging to the technical field of traditional Chinese medicine processing.
Background
The natural tea forest is characterized in that lithocarpus polystachyus, lithocarpus of Fagaceae, namely sweet tea, stevia rebaudiana, fatness tea, sweet tea, rhizia macrophylla, glycoside tea, lithocarpus polystachyus and the like are scattered in a low mountain dense forest in southern province of Changjiang river of China in a wild form, wherein the snow mountain in the south of Hunan province has Huaxiao and the opening, the yield of the district of Huahui Yao Yaoshan is the most, and the relatively rare natural tea forest has a plurality of parts in the natural protection area (new town) of the Yaohong county at the opening. The lithocarpus polystachyus belongs to a medicine-food homologous plant and has three functions of tea, sugar and medicine. The tender leaves of Lithocarpus polystachyus (hook. f.) Rehd after soaking have high sweet taste, and are called sweet tea. Lithocarpus polystachyus (wall.) Rehd has long drinking history as sweet tea, and ancient people mostly have the functions of clearing heat and removing toxicity, soothing nerves and reducing blood pressure. In recent years, the component detection of lithocarpus polystachyus rehd products shows that the sweet taste of the lithocarpus polystachyus rehd products comes from phlorizin components, the content of the phlorizin components reaches 12.6%, and the lithocarpus polystachyus rehd products belong to dihydrochalcones in flavone compounds. Experiments of the institute of pharmacy in Chinese academy of sciences show that phlorizin can well inhibit the absorption of sugar by human bodies and has the function of repairing insulin in the human bodies. The lithocarpus polystachyus rehd has the total flavonoid component content of 22.2 percent, the triterpenoid compound content of 2.25 percent and the polyphenol compound content of 6.18 percent, has good lipid-lowering, anti-tumor, anti-oxidation and anti-allergic effects, and is known as 'tree cordyceps'.
Phlorizin is a characteristic polyphenol component in apples, widely exists in tissues such as apple peels, apple pulp, seeds, tree roots, stems, branches, leaves and the like, and has various biological activities and functions of reducing blood sugar, preventing diabetic complications, inhibiting tyrosinase activity, resisting allergy, oxidation and aging, improving memory function, preventing osteoporosis, reducing blood cholesterol level, protecting liver and the like.
Disclosure of Invention
The invention aims to provide a preparation method for extracting phlorizin from lithocarpus polystachyus rehd, which is characterized by comprising the following preparation process flows:
a preparation method for extracting phlorizin from lithocarpus polystachyus rehd is characterized by comprising the following preparation process flows:
1) selecting raw materials, cleaning, removing impurities, drying and crushing;
2) soaking the raw materials in 70-90% ethanol solution for 1-2 h;
3) ultrasonic-assisted extraction for 30-60min at 400-600 w/w at 30-80 deg.C for 2-3 times;
4) filtering to remove residues, mixing filtrates, adsorbing with macroporous resin column, and eluting with 90% ethanol solution;
5) recovering ethanol, concentrating under reduced pressure at 55-65 deg.C under-0.8 MPA, and drying to obtain Lithocarpus polystachyus (wall.) Rehd phlorizin.
The method for extracting phlorizin from lithocarpus polystachyus rehd as claimed in claim 1, wherein the amount of ethanol solution for soaking the raw material is 5-8 times of that of the raw material.
The method for preparing phlorizin extracted from lithocarpus polystachyus as claimed in claim 1, wherein the volume ratio of the ethanol solution used as eluent to the raw material solution is 1: 20-25, slowly passing through a macroporous resin column at an elution speed of 2-3ml/min, wherein the model of the selected macroporous resin is D101.
The specific implementation mode is as follows:
example 1
A preparation method for extracting phlorizin from lithocarpus polystachyus rehd is characterized by comprising the following preparation process flows:
1) selecting raw materials, cleaning, removing impurities, drying and crushing;
2) soaking the raw materials in 70-90% ethanol solution for 1-2 h;
3) ultrasonic-assisted extraction for 30-60min at 400-600 w/w at 30-80 deg.C for 2-3 times;
4) filtering to remove residues, mixing filtrates, adsorbing with macroporous resin column, and eluting with 90% ethanol solution;
5) recovering ethanol, concentrating under reduced pressure at 55-65 deg.C under-0.8 MPA, and drying to obtain Lithocarpus polystachyus (wall.) Rehd phlorizin.
The method of claim 1, wherein the amount of ethanol solution used to soak the material is 5-8 times the amount of ethanol solution used to soak the material.
The method for preparing phlorizin extracted from lithocarpus polystachyus as claimed in claim 1, wherein the volume ratio of the ethanol solution used as eluent to the raw material solution is 1: 20-25, slowly passing through a macroporous resin column at an elution speed of 2-3ml/min, wherein the model of the selected macroporous resin is D101.
Example 2
A preparation method for extracting phlorizin from lithocarpus polystachyus rehd is characterized by comprising the following preparation process flows:
1) selecting raw materials, cleaning, removing impurities, drying and crushing;
2) soaking the raw materials in 70-90% ethanol solution for 1-2 h;
3) ultrasonic-assisted extraction for 40-80min at power of 200-500 w at 50-80 deg.C, and repeating for 2-3 times;
4) filtering to remove residues, mixing filtrates, adsorbing with macroporous resin column, and eluting with 70% ethanol solution;
5) recovering ethanol, concentrating under reduced pressure at-0.5 MPA and 60-70 deg.C, and drying to obtain Lithocarpus polystachyus (wall.) Rehd phlorizin.
The method for extracting phlorizin from lithocarpus polystachyus rehd as claimed in claim 1, wherein the amount of ethanol solution for soaking the raw material is 5-8 times of that of the raw material.
The method for preparing phlorizin extracted from lithocarpus polystachyus as claimed in claim 1, wherein the volume ratio of the ethanol solution used as eluent to the raw material solution is 1: and 25, slowly passing through a macroporous resin column at an elution speed of 2-2.5ml/min, wherein the model of the selected macroporous resin is D101.
Claims (3)
1. A preparation method for extracting phlorizin from lithocarpus polystachyus rehd is characterized by comprising the following preparation process flows:
selecting raw materials, cleaning, removing impurities, drying and crushing;
soaking the raw materials in 70-90% ethanol solution for 1-2 h;
ultrasonic-assisted extraction for 30-60min at 400-600 w/w at 30-80 deg.C for 2-3 times;
filtering to remove residues, mixing filtrates, adsorbing with macroporous resin column, and eluting with 90% ethanol solution;
recovering ethanol, concentrating under reduced pressure at 55-65 deg.C under-0.8 MPA, and drying to obtain Lithocarpus polystachyus (wall.) Rehd phlorizin.
2. The method for extracting phlorizin from lithocarpus polystachyus rehd as claimed in claim 1, wherein the amount of ethanol solution for soaking the raw material is 5-8 times of that of the raw material.
3. The method for preparing phlorizin extracted from lithocarpus polystachyus as claimed in claim 1, wherein the volume ratio of the ethanol solution used as eluent to the raw material solution is 1: 20-25, slowly passing through a macroporous resin column at an elution speed of 2-3ml/min, wherein the model of the selected macroporous resin is D101.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811355921.4A CN111187314A (en) | 2018-11-15 | 2018-11-15 | Preparation method for extracting phlorizin from lithocarpus polystachyus rehd |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811355921.4A CN111187314A (en) | 2018-11-15 | 2018-11-15 | Preparation method for extracting phlorizin from lithocarpus polystachyus rehd |
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| Publication Number | Publication Date |
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| CN111187314A true CN111187314A (en) | 2020-05-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811355921.4A Withdrawn CN111187314A (en) | 2018-11-15 | 2018-11-15 | Preparation method for extracting phlorizin from lithocarpus polystachyus rehd |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112225768A (en) * | 2020-11-18 | 2021-01-15 | 成都农业科技中心 | Method for extracting trilobatin and phlorizin from lithocarpus litseifolius |
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2018
- 2018-11-15 CN CN201811355921.4A patent/CN111187314A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112225768A (en) * | 2020-11-18 | 2021-01-15 | 成都农业科技中心 | Method for extracting trilobatin and phlorizin from lithocarpus litseifolius |
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Legal Events
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200522 |