CN109419830A - Taiwan Chenopodium quinoa shell extract with whitening and antiaging effects, and its extraction and separation method - Google Patents
Taiwan Chenopodium quinoa shell extract with whitening and antiaging effects, and its extraction and separation method Download PDFInfo
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- CN109419830A CN109419830A CN201810340106.4A CN201810340106A CN109419830A CN 109419830 A CN109419830 A CN 109419830A CN 201810340106 A CN201810340106 A CN 201810340106A CN 109419830 A CN109419830 A CN 109419830A
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- 210000001652 frontal lobe Anatomy 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000010437 gem Substances 0.000 description 1
- 229910001751 gemstone Inorganic materials 0.000 description 1
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- 210000004072 lung Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000009707 neogenesis Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 230000008845 photoaging Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000004215 skin function Effects 0.000 description 1
- 230000036559 skin health Effects 0.000 description 1
- 231100000046 skin rash Toxicity 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
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- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A61Q19/08—Anti-ageing preparations
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention relates to a Taiwan Chenopodium album extract method which is helpful for whitening and anti-aging, a composition obtained by extracting and purifying the extract of the Taiwan Chenopodium album can be used for resisting oxidation, whitening or resisting skin aging; which comprises the following steps: sequentially carrying out alcohol extraction, filtration and drying on the Taiwan Chenopodium album shells to obtain a primary extract; the preliminary extract may optionally be subjected to further purification or differentiation steps to separate the components of different nature of the preliminary extract or used directly in the product.
Description
Technical field
The present invention be for a kind of Taiwan Chenopodiaceae shell extracting process, it is especially a kind of resulting to concentrate and purify using alcoholic extract
Taiwan Chenopodiaceae shell extract, the subsequent extract distinguished using chromatography scheme, has by being applied to skin or eating the extract
Help whitening and anti-aging.
Background technique
Taiwan Chenopodiaceae (Chenopodium formmosanum), also known as red goosefoot, red Chenopodiaceae, purple Chenopodiaceae, rainbow rice, are Amaranthaceae Chenopodiaceae
The Taiwan initial species plant of subfamily Chenopodium belongs to annual herb plant, is the traditional crop of Taiwan aboriginal's farming a century or more,
Growth characteristics are drought-enduring, resistance to high salt.In aboriginal's tradition using upper, will be mostly total to after the grain decladding of Taiwan Chenopodiaceae with coarse cereals such as rice
It boils, cooking is used at Zongzi, bamboo tube rice, or brewing millet wine.Chenopodiaceae plant is edible in Taiwan, and seedling, tender leaf and flower spike enter dish；
Separately in terms of the Chinese herbal medicine, Taiwan Chenopodiaceae complete stool can be used as medicine, and have and dry, heat-clearing, removing toxic substances, antidiarrheal and improve skin and the functions such as itch.Platform
Gulf Chenopodiaceae is food materials full of nutrition rich in splendid and comprehensive nutrient, minerals and a variety of polyphenoils, is known as that " cereal is red
The good name of jewel ".
The functional components of Taiwan Chenopodiaceae all have anti-oxidant, anti-inflammatory and anticancer based on flavones, phenols and beet pigments
Equal physiological activity.Far more than general cereal, Polyphenols also has there are many oxidation resistant the vegetalitas Polyphenols content of Taiwan Chenopodiaceae
Effect, can anti-inflammatory, inhibition artery sclerosis and thrombosis, blood pressure lowering, maintenance blood vessel elasticity, promotion insulin secretion, inhibition cancer
Hyperplasia etc., and aging can be prevented, maintain elasticity of skin；In the Chenopodiaceae of Taiwan flavonoid substances abundant similarly help to remove from
By base, anti-oxidant, softening blood vessel is helped avoid, can promote glucose-lipid metabolism and insulin secretion.
The grain of Taiwan Chenopodiaceae is more after harvesting to remove shell russet, because of " soap of the Taiwan Chenopodiaceae shell containing micro- toxicity
Element ", bitter, poor taste can be felt by causing band shell to eat, not easy to be processed edible, also make the limit for having intake with shell Taiwan Chenopodiaceae
System, therefore utilization of the Taiwan Chenopodiaceae shell on eating is very limited, and the Taiwan Chenopodiaceae shell of research discovery in the past accounts for about the 27% of full seed,
Chenopodiaceae shell removal in Taiwan will be lost into nearly three one-tenth agricultures business output, how to efficiently use Taiwan Chenopodiaceae shell as important project to be solved.
To sum up, Taiwan Chenopodiaceae had both been excellent food grains other than rice and wheat, and edible position is carried out extraction using actually wasting, if can be single
Inedible Taiwan Chenopodiaceae shell is solely utilized, resource will be more effectively utilized.Therefore the present invention is utilized in a manner of alcoholic extract
Taiwan Chenopodiaceae shell, being purified into facilitates skin-whitening, anti-aging, crease-resistant Taiwan Chenopodiaceae shell extraction differentiator.
Melanocyte (melanocytes) in order to protect injury of the skin not by sunlight middle-ultraviolet lamp, in skin
Meeting synthesis of melanin (melanin) avoids ultraviolet light from damaging cell and inhereditary material to resist ultraviolet light.Past research
It points out that Tyrosinase (tyrosinase) is the key that modulating melanin generates ferment, is not only involved in during melanin production
Many reactions, and be the key that limiting speed step ferment in catalysis reaction, therefore the content of Tyrosinase can be used as black
Element generates the index of reaction.Since Melanin productions increase will lead to, the colour of skin is dim to sink, and makes one to seem to lack spirit in perception,
Therefore how skin care to slow down melanin production or skin is made to restore pale, becomes the direction pursued for numerous consumers.
There are many whitening agents (such as: hydroquinone and mercury) for cosmetics and medicine, will cause skin pigment after use
Abnormal phenomenon, such as allergic reaction, rubefaction, skin rash, the skin of bacterium and fungal infection is resisted in discoloration, and reduction
The side effects such as ability, or even Toxicity of Kidney can be caused, therefore researcher seeks safe and effective natural goods constantly to develop whitening
Product.Confirm that the natural materials with whitening effect for example have kojic acid (kojic acid), ellagic acid (ellagic through research
Acid) and Arbutin (arbutin) etc., above-mentioned three all belongs to Tyrosinase inhibitor.Although these substances are for whitening
Truly have significant effect, but respectively there is a limitation in its use, for example, highly unstable be oxidized easily of kojic acid and change colour and cause
Skin allergy, and kojic acid product is excessively used for a long time and will lead to cytotoxicity and lesion occurs；In addition, the effect of some whitening agents
It is direct destruction melanocyte, easily causes the poisoning of cell.Therefore, how to find out can be used for inhibiting melanin and has
The effective component of safety becomes as an important topic.
Aging be physiological status at any time process deteriorate the phenomenon that, aging is the process of biological aging.The generation of aging can
Observe that this molecular damage causes old permitted from molecule stratum, such as inhereditary material, protein, the equimolecular variation of lipid
More diseases.In fact, there are many mechanism of self-regeneration free radical injury in organism, however when maintenance speed is not as good as free radical
When injuring speed, impaired molecule is easy for accumulating, and further results in disease, gradually grows and increase the disease of Probability with the age
Shape includes skin aging, arthritis, cancer, Alzheimer etc..It is most popular theoretical for " free basis mechanism about aging
By ".Variation accumulation caused by high activity molecule initiation reaction of the theoretical hypothesis aging by being referred to as " free radical " in body
And it generates.It is widely believed that the main reason for variation induced by free radical is aging and disease development at present.Normal physiology generation
During thanking, free radical multi-source is in physiologically grain wire body electron transport chain (electron transport chain), peroxidating
(peroxidase), it is generated in the systems such as cell chromaticness P450 (cytochrome P-450), metabolism generates energy and provides biology
With；In fact, with the balance on maintenance metabolism, however working as in organism there are many mechanism of self-regeneration free radical injury
When maintenance speed is not as good as free radical injury speed, impaired molecule is easy for accumulating, and further results in aging or disease.Age
Gradually long, metabolism decline is influenced by abnormal environment, such as: ultraviolet light, smoking, air pollution or even psychological pressure, life are not
All the balance Libra of free radical may normally tilted to aging direction.
It further illustrates, free radical generates damage to bio-genetic material and increases gene mutation chance, and then increases cancer
Disease disease incidence.Likewise, free radical will lead to low density cholesterol (LDL) excessive oxidation to the damage of lipid molecular and generate dynamic
Arteries and veins patch, and then may cause atherosclerosis and other cardiovascular diseases.In addition, free radical can cause protein molecule to damage
Wound leads to interaction connection (cross linking), amyloid formation and brain cell variation, and then can cause A Zihaimoshi
Disease or the disease incidence for increasing Alzheimer.Free radical can also cause to lead to arthritic tissue to the damage of protein molecule
Structure change and autoimmune disorder and influence skin function and the collagen of appearance, elastomer are impaired.Therefore how
It is anti-oxidant, reduce interior free yl, reaching prevention disease and postponing cell senescence is the one big of contemporary health care biotechnology
Development priority.
On the other hand, saccharification (Glycation) refers to that glucose or fructose are attached on protein, makes the egg of saccharification
White matter changes structure and property, becomes easy aggregation and is staggeredly coupled.The position that saccharification occurs is random, it is considered that freely
Base concentration height will cause higher saccharification probability, and research at present thinks that protein is excessively saccharified the final glycated protein of generation
(Advanced Glycation End-products, AGEs), has substantial connection with aging and part disease.When sugared in body
When change effect generates too many final glycated protein, easily lead to the different lesions at each position, as eyes may be easy fatigue, it is dry and astringent very
To the risk for improving cataract and retinopathy；Final glycated protein caused by saccharification can be also deposited on vascular wall, and
It induces interior free yl to generate, artery is hardened, tube wall thickens for acceleration；When the accumulation of final glycated protein is when bone, bone can become
It is fragile, cell neogenesis mechanism is suppressed, accelerate osteoporosis the case where.In addition, root is it was found that Alzheimer's disease patient's brain
Protein in portion's frontal lobe, compared with healthy old men, final glycated protein is up to 3 times or more, illustrates to accumulate final saccharification in vivo
Albumen has correlation with many diseases.
Saccharification, which betides, is considered as one of skin aging, important mechanisms of relaxation on the protein of dermal layer of the skin.
Collagen (Collagen) is a kind of important protein of human body, accounts about the 25~35% of total protein, is primarily present
It is intercellular bracket in connective tissue, there is very strong stretch capability, be widely present skin, cornea, joint, cartilage etc.
In tissue.Especially in skin, collagen is the protein mainly supported, it is full to be able to maintain skin-tightening.And collagen egg
White saccharification can make collagen fibrosis and become stiff, and skin loses filling, accelerates the generation of wrinkle of skin.
In addition, elastin laminin (Elastin) be it is a kind of constitute elastomer protein, it is widely distributed in vivo
Each tissue and internal organs in, be primarily present in the flexible tissue such as skin, ligament, blood vessel, lung, skin.And this kind of group
It knits and not only needs elasticity, while tensile strength is also essential, and elastin laminin plays the composition of form and maintenance
Important role.Elastin laminin is also the important component of skin composition, and it is generation bullet that elastin laminin contains only 5% in skin
Property fiber and support collagen key.Elastin laminin molecule can be crimped arbitrarily, under external force drawing, the elastin laminin of curling
Molecular stretching elongates, and after removing external force, it is rolled state that elastin laminin molecule is replied again, just as spring.Human body elastin laminin master
It to be synthesized early stage in late embryo stage to newborn, adult life almost no longer has new elastin laminin to generate.Account for skin 70%
Collagen determines the soft and moist full of skin, and elastomer then determines the compact elastic force of skin.Same saccharification and light aging
Journey (photoaging), which also results in elastomer, can generate qualitative change, to lose original elasticity.Elastoser
It (Elastase) is a kind of ferment that the elastin that can be allowed in connective tissue decomposes, the reduction of elastin will lead to wrinkle
And sagging equal agings sign.Therefore the reduction and destruction for how reducing elastin laminin are anti-aging, crease-resistant, maintenance skin healths
The compact subject under discussion to merit attention.
In summary, the collagen generative capacity of replenishing collagen or promotion cell, and inhibit elastic egg
White decomposition is prevention or the important method for slowing down skin aging, can more be prevented because of the protein accumulation being saccharified and derivative phase
Related disorders.There is the Related product of collagen on the market at present, all using direct replenishing collagen as major function, however,
The molecule of collagen is big, and the cuticula that smearing mode can not almost make collagen penetrate skin reaches its corium acted on
Layer dermal fibroblasts；If the direct replenishing collagen in a manner of injection, although the microgroove of skin can be reduced immediately,
The injection enters intracorporal collagen and is easily decomposed by intracorporal ferment, needs regularly injection, process is complicated and at high cost, and infuses
Penetrating the issuable immunological rejection of collagen is even more a Risks.If being mended with eating collagen-rich food
Collagen is filled, after collagen enters stomach, cuts into small molecule, last breaks down into amino acids via digestive juice.Body is solid
These Amino acid synthesis protein can be so utilized, but are not limited to collagen, therefore the effect of its practical replenishing collagen has
Limit.On the other hand, the product of elastin laminin, smearing mode, which can not equally supplement, substitutes reduced elastin laminin or fiber；Research
It was found that intracorporal elastin laminin can not almost regenerate, it is existing still to generate elastin laminin in the Vitro Culture Techniques of conceptual phase
It implants again after structure, but the technology is not yet mature.Therefore inhibit elastin laminin to decompose enzymatic activity to be avoided that as minority
Elastin laminin reduces the important channel for leading to aging, skin sagging.
Summary of the invention
It is extracted the present invention is characterized in Taiwan Chenopodiaceae shell extract and the constituent obtained after purification can be used for resisting
Oxidation, whitening or resisting age of skin.
Another extracting process for being characterized mainly in that Taiwan Chenopodiaceae shell alcoholic extract of the invention, which comprises the steps of:, takes platform
Gulf Chenopodiaceae shell generates preliminary extract after sequentially carrying out alcoholic extract, filtering and drying；The preliminary extract selectively carries out
The step of being further purified or distinguishing is so that the composition of different nature of the preliminary extract separates or be used directly for product
In.
Detailed description of the invention
Fig. 1 is the extraction of Taiwan Chenopodiaceae shell alcoholic extract and the flow chart of differentiating method；
Fig. 2-1 is Taiwan Chenopodiaceae shell alcoholic extract and its differentiator on the extracellular active influence of elastin laminin catabolic enzyme；
Fig. 2-2 be under 0.1mg/L concentration Taiwan Chenopodiaceae shell alcoholic extract and its differentiator to ultraviolet light (UVB)
(500mJ/cm2) induced skin fibroblast elastin laminin catabolic enzyme maximum inhibition influence；
Fig. 3 is Taiwan Chenopodiaceae shell alcoholic extract and its influence that differentiator forms melanin；
Fig. 4 is the influence of Taiwan Chenopodiaceae shell alcoholic extract and its differentiator to the cell survival rate of melanocyte；
Fig. 5 is that Taiwan Chenopodiaceae shell alcoholic extract and its differentiator induce melanocyte to α-melanocyte-stimulating hormone
The influence of melanin forming amount；
Fig. 6 is that Taiwan Chenopodiaceae shell alcoholic extract and its differentiator induce melanocyte to α-melanocyte-stimulating hormone
The active influence of Tyrosinase；
Fig. 7 is the ability that Taiwan Chenopodiaceae shell alcoholic extract and its differentiator remove DPPH free radical；
Fig. 8 is the influence of Taiwan Chenopodiaceae shell alcoholic extract and its differentiator to final glycated protein Forming ability is inhibited.
Specific embodiment
Taiwan Chenopodiaceae shell is taken, is cleaned, powder is ground to physical method after drying；Take resulting Taiwan Chenopodiaceae shell
Powder is put into alcohol and is concentrated, and obtains preliminary extract.
Preferably, after taking Taiwan Chenopodiaceae shell powder that alcohol concentration is added, the preliminary extract of gained time is soluble in water, first it is added
Organic solvent carries out layering extracted several times, with the chaff interferent gone in oil removing phase；Then phase extract liquor of fetching water again concentration, can with reduction
The organic solvent in extract can be stayed in, the extract purified.The Taiwan Chenopodiaceae shell alcohol of purifying extraction can directly be utilized
Extract (EEDH), or continue to distinguish using chromatography according to demand, the differentiator of different condition is used according to demand,
In, the differentiating method can be not limited to chromatography.Wherein, the organic solvent of layering extraction can be ether, methylene chloride, chlorine
Imitative, carbon tetrachloride, benzene, isoamyl alcohol, n-butanol, pentane and n-hexane etc..
Preferred embodiment is for example are as follows: takes Taiwan Chenopodiaceae powder, carries out four times of concentrations after being dissolved in 95% alcohol, tentatively extracted
Take liquid；After filtering preliminary extract liquor and removing alcohol, by extract with secondary water back dissolving；N-hexane is added to be layered
Extracted several times, to remove oil phase substance.The aforementioned water phase extract liquor (EEDH) being obtained by extraction that is layered is taken to carry out tubing string chromatographic analysis,
Water differentiator (EEDH-W), 50% alcohol differentiator (EEDH-50) and 95% alcohol differentiator (EEDH-95) are obtained, and is not managed
The Taiwan Chenopodiaceae shell alcoholic extract (EEDH) of column chromatography carries out follow-up test and analysis, as a result such as Fig. 2-1
Fig. 2-1 is extracellular elastin laminin catabolic enzyme active suppression test, with Taiwan Chenopodiaceae shell alcoholic extract and differentiator into
Row test finds that Chenopodiaceae shell 95% alcohol differentiator (EEDH-95) in Taiwan is Taiwan Chenopodiaceae shell alcoholic extract by Fig. 2-1 result of study
The active differentiator of optimal inhibition elastin laminin catabolic enzyme, inhibitory effect increase with the increase of its concentration in differentiator,
Its IC50About 0.1mg/mL is much better than the rejection ability (1.0mg/ of the Taiwan Chenopodiaceae shell alcoholic extract (EEDH) before not differentiating between
ML), remaining differentiator then has no that significant inhibition elastin laminin decomposes enzymatic activity.
Opposite, with ultraviolet light (UVB 500mJ/cm2) induced skin fibroblast (HaCat) elastoser
In active suppression test, as a result such as Fig. 2-2, the elastic egg of Taiwan Chenopodiaceae shell alcoholic extract under the concentration of 0.1mg/mL is found
White decomposition inhibition of enzyme activity ability is about 28.2%, but after col-umn chromatography, 50% alcohol differentiator (EEDH- of Taiwan Chenopodiaceae shell
50) and the elastin laminin of 95% alcohol differentiator (EEDH-95) decompose inhibition of enzyme activity ability increase separately to 60.5 and
76.3%, show the ability that Taiwan Chenopodiaceae shell alcoholic extract decomposes after col-umn chromatography with preferable elastin laminin.
It cultivates melanoma cells (B16F10) and applies different reagent test melanin rejection abilities, it is of the invention to assess
Whitening capability, Taiwan Chenopodiaceae shell alcoholic extract and its differentiator induce black with 1 μM of α-melanocyte-stimulating hormone (α-MSH)
Plain oncocyte forms the influence of melanin ability.Test result such as Fig. 3, microscope magnifications 100X.
By Fig. 3 the results show that α-melanocyte-stimulating hormone and melanoma cells culture six days, significant promotion melanin
It is formed, 200 μM of kojic acid (Kojic acid) then has the tendency that significant inhibition melanin formation.Taiwan Chenopodiaceae shell wine extracts object (EEDH)
Under the concentration of 50-200 μ g/mL, the significant decline of melanin forming amount, and its 50% alcohol differentiator (EEDH-50) and 95%
Alcohol differentiator (EEDH-95) is also in significant downward trend.
Concentration according to Taiwan Chenopodiaceae shell alcoholic extract and its differentiator is divided into 50,100,200,500 and 1000ug/mL five
Group tests the cell survival rate of melanocyte the safety of the extract, by result figure 4 it is found that Taiwan Chenopodiaceae shell wine extracts object
(EEDH), water differentiator (EEDH-W) and 50% alcohol differentiator (EEDH-50) do not have significant cell toxicant in 200 μ g/mL
Property, cell survival rate > 85%, therefore three kinds of samples select to carry out with the following concentration of 200 μ g/mL in follow-up test, and 95% wine
Smart differentiator (EEDH-95) has very significant cytotoxicity in 200 μ g/mL, therefore the following concentration of 100 μ g/mL is selected to carry out
The Tyrosinase activity and melanin for inhibiting melanoma cells form test.
Then further analysis Taiwan Chenopodiaceae shell alcoholic extract and its differentiator induce 1 μM of α-melanocyte-stimulating hormone
The Tyrosinase activity of melanoma cells and the influence of melanin forming amount, Fig. 5,6 are the results show that Taiwan Chenopodiaceae shell wine extracts object
(EEDH) the inhibition Tyrosinase activity under 200 μ g/mL concentration and melanin Forming ability respectively may be about 60% and 50%, three
A differentiator all has significant inhibition Tyrosinase activity and melanin Forming ability, effect to be superior to 200 μM of kojic acid.Its
In with the inhibitory effect of 50% alcohol differentiator (EEDH-50) be it is best, apparent dosage effect is presented, in 200 μ g/mL concentration
Under inhibition Tyrosinase activity and melanin Forming ability up to the inhibitory effect that respectively may be about 84% and 86%.
The anti-aging and anti-saccharification capability of Taiwan Chenopodiaceae shell alcoholic extract and its differentiator is further analyzed, respectively to remove
Two tests of influence of free radical ability and final glycated protein (AGEs) Forming ability carry out Taiwan Chenopodiaceae shell alcoholic extracts and
The resistance to oxidation of its differentiator and the assessment of anti-saccharification capability.
Scavenging ability is measured with hydrogen supply capacity, is to utilize 1,1- diphenyl -2- trinitrophenyl-hydrazine (referred to as
DPPH) free radical scavenging ability (scavenging effect) indicates.Take 4ml extract, add 1mL Fresh 1.0 ×
10-4The methanol solution of mM DPPH uniformly mixes, and after standing 30 minutes, detects the light absorption value of 517nm, as a result such as Fig. 7.
By Fig. 7 the results show that the water differentiator of Taiwan Chenopodiaceae shell alcoholic extract and Taiwan Chenopodiaceae shell alcoholic extract, 50% wine
Smart differentiator has quite good scavenging ability of DPPH free radical, other than 95% alcohol differentiator, presents significant dense
Depression effect is spent, wherein it is best (IC50 is approximately equal to 1.5mg/mL) with the effect of 50% alcohol differentiator (EEDH-50), be secondly
The water differentiator (EEDH-W) of Taiwan Chenopodiaceae shell alcoholic extract, Taiwan Chenopodiaceae shell alcoholic extract (EEDH), in the concentration of 2mg/mL
Under, the DPPH clearance rate of three is followed successively by 66.7,45.6 and 33.5%.
The influence of Taiwan Chenopodiaceae shell alcoholic extract and its differentiator to final glycated protein Forming ability, by 10 μ L extracts
The 50mg/mL bovine serum albumin(BSA) BSA (being dissolved in the phosphate buffered saline for including 0.05% sodium azide) of 740 μ L is added
And 250 μ L 3M glucose be uniformly mixed, in 37 DEG C react 3 weeks after, using fluorescence detector excitation wavelength 330nm, radiation
Under conditions of wavelength 410nm, fluorescent value is measured, as a result such as Fig. 8
By Fig. 8 result, it is found that Taiwan Chenopodiaceae shell alcoholic extract is under 1000 μ g/mL concentration, only there are about 30% inhibition is final
The ability that glycated protein is formed, best with 50% alcohol differentiator (EEDH-50) expressive ability in differentiator, there are about 65% suppressions
Effect processed, in the known saccharification inhibitor aminoguanidine of inhibition final the glycated protein Forming ability and 1mM of 500 μ g/mL
(Aminoguanidine) quite.But remaining differentiator does not have the significant final glycated protein Forming ability of inhibition then.
Taiwan Chenopodiaceae effective component is further analyzed, as the Taiwan the Quan Gu Chenopodiaceae, shelling Taiwan Chenopodiaceae and Taiwan Chenopodiaceae shell of table 1 are distinguished
The quantitative comparison of effective component is taken out by water or alcoholic extract.As shown in Table 1, alcoholic extract is in total polyphenols, general flavone and white Chenopodiaceae
There is preferable effect of extracting in the extraction of reed alcohol；And the effective component of Quan Gu, shelling and Taiwan Chenopodiaceae shell, with Taiwan Chenopodiaceae shell content
It is the abundantest, and it is most with the active constituent content of alcoholic extract.
The active constituent content ratio of 1 Taiwan Quan Gu Chenopodiaceae of table, the water of shelling Taiwan Chenopodiaceae and Taiwan Chenopodiaceae shell or alcoholic extract
Compared with
By past many researchs it is found that flavonoids in Polyphenols (polyphenol) and phenolic acid mostly have it is anti-oxidant,
Anti-inflammatory, anticancer, the anti-aging and abilities such as promotion skin-whitening, consolidation, crease-resistant, wherein rutin sophorin (rutin) is to skin care
Function human clinical trial confirm, also without apparent side effect.
The class polyphenol content for further comparing Taiwan Chenopodiaceae shell alcoholic extract and 50% alcohol differentiator, after col-umn chromatography
The Polyphenols composition of 50% alcohol differentiator (EEDH-50) about promote 20 times or so, this trend and whitening after distinguishing and anti-
The promotion of aging ability is consistent, it is therefore evident that main whitening and anti-aging functional ingredient should be rutin sophorin in red goosefoot shell
(rutin), ferulic acid (ferullic acid), vanillic acid (vanillic acid) and P- coumaric acid (p-coumaric
The Polyphenols composition such as acid).
2 Taiwan Chenopodiaceae shell alcoholic extract of table and its class polyphenol content of differentiator compare
Note: N.D. is to be not detected
In summary as a result, Taiwan Chenopodiaceae shell alcoholic extract (EEDH) is removing free radical, inhibiting saccharification capability, extracellular junket
In amino acid enzyme and extracellular elastin laminin catabolic enzyme Activity Assessment ability, significant inhibitory effect is all had；And pass through col-umn chromatography
Resulting differentiator is in various anti-oxidant, whitenings and ageing resistance assessment after separation, significant promotion.Wherein with 50% wine
The anti-oxidant anti-saccharification of smart differentiator (EEDH-50) and whitening capability are best；But in extracellular and Skin Cell different mode system
It with 95% alcohol differentiator (EEDH-95) and 50% alcohol differentiator (EEDH-50) is respectively best in system.The production of later use
Product can select suitable section differentiator as raw material according to purposes, efficacy requirements.
Claims (8)
1. a kind of Taiwan Chenopodiaceae shell alcoholic extract, the constituent which obtains through extraction and after purification can be used for it is anti-oxidant,
Whitening or resisting age of skin.
2. Taiwan Chenopodiaceae shell alcoholic extract as described in claim 1, wherein the constituent can be used as externally applied liniment, external application
The beginning raw material of pharmaceuticals, external application skin care products or cosmetics.
3. Taiwan Chenopodiaceae shell alcoholic extract as described in claim 1, wherein the constituent can be used as food or health care
The beginning raw material of product.
4. Taiwan Chenopodiaceae shell alcoholic extract as described in claim 1, wherein the extraction are as follows: take Taiwan Chenopodiaceae shell, successively carry out
Preliminary extract is obtained after alcoholic extract, filtering and drying.
5. Taiwan Chenopodiaceae shell alcoholic extract as described in claim 1, wherein the purifying is is further purified or area after extraction
The composition of different nature of the preliminary extract is set to separate or be used directly in product the step of dividing.
6. a kind of extracting process of Taiwan Chenopodiaceae shell alcoholic extract, the method includes the steps of:
Extraction step: taking Taiwan Chenopodiaceae shell, obtains preliminary extract after successively carrying out alcoholic extract, filtering and drying；
Purification step: the step of preliminary extract is selectively further purified or is distinguished is so that the preliminary extraction
The composition of different nature of object is separated or is used directly in product.
7. the extracting process of Taiwan Chenopodiaceae shell alcoholic extract as claimed in claim 4, wherein the purification step is by dividing
Layer extraction separates unwanted chaff interferent.
8. the extracting process of Taiwan Chenopodiaceae shell alcoholic extract as claimed in claim 4, wherein to pass through the step of the differentiation
Effective ingredient is separated and is concentrated by chromatography, and the chromatographic solution of col-umn chromatography used is differentiation the step of differentiation described in alcohol
Concentration is 50-95%.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112237590A (en) * | 2019-07-19 | 2021-01-19 | 百岳特生物技术（上海）有限公司 | Chenopodium formosanum juice, its application and fat reducing composition |
| WO2022141182A1 (en) * | 2020-12-30 | 2022-07-07 | 财团法人医药工业技术发展中心 | Pharmaceutical composition for treating alcoholic fatty liver and use thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922171A (en) * | 2014-03-20 | 2015-09-23 | 百岳特生物科技（上海）有限公司 | Use of extract of Chenopodium quinoa for preparing composition for promoting collagen production and resisting skin aging |
| TWM539335U (en) * | 2016-11-30 | 2017-04-11 | 大葉大學 | Patch structure with Chenopodium formosanum extract dressing |
| TW201811353A (en) * | 2016-09-29 | 2018-04-01 | 大葉大學 | Preparing method of Chenopodium formosanum extracts and dressing composition |
-
2017
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922171A (en) * | 2014-03-20 | 2015-09-23 | 百岳特生物科技（上海）有限公司 | Use of extract of Chenopodium quinoa for preparing composition for promoting collagen production and resisting skin aging |
| TW201811353A (en) * | 2016-09-29 | 2018-04-01 | 大葉大學 | Preparing method of Chenopodium formosanum extracts and dressing composition |
| TWM539335U (en) * | 2016-11-30 | 2017-04-11 | 大葉大學 | Patch structure with Chenopodium formosanum extract dressing |
Non-Patent Citations (1)
| Title |
|---|
| PI-JEN TSAI，等: "Effect of Nanogrinding on the Pigment and Bioactivity of Djulis(Chenopodium formosanum Koidz.)", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112237590A (en) * | 2019-07-19 | 2021-01-19 | 百岳特生物技术（上海）有限公司 | Chenopodium formosanum juice, its application and fat reducing composition |
| WO2022141182A1 (en) * | 2020-12-30 | 2022-07-07 | 财团法人医药工业技术发展中心 | Pharmaceutical composition for treating alcoholic fatty liver and use thereof |
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