CN105614071A - Soybean isoflavone extract and extraction method thereof - Google Patents

Abstract

The invention discloses a soybean isoflavone extract. The soybean isoflavone extract is prepared from, by weight, 1.8-10% of daidzin, 0.1-1% of soybean xanthosine, 0.8-8% of genistin, 2.1-12% of isoflavoues aglycone, 0.1-1% of glycitein and 0.2-2% of genistein. It is shown by effect experiments that productive performance of the piglets is obviously improved after the soybean isoflavone extract is added into feed to feed piglets. Compared with an existing soybean isoflavone product, the extract has remarkable advantages, indexes such as daily gain, average daily feed intake and the feed conversion ratio are higher than those of the commercially available product Meilidun on the same feeding conditions, and the extract has good market prospects in the field of livestock and poultry breeding. The invention further provides a preparing method of the soybean isoflavone extract. The preparing method is simple in extraction technology, short in extraction time, low in extraction temperature, low in energy consumption and beneficial for large-scale production and reduces production cost to the maximum, and solvents in use can be used for fast extracting special components and are small in use amount.

Description

A kind of soybean isoflavone and extracting method thereof

Technical field

The present invention relates to a kind of soybean isoflavone and extracting method thereof, belong to field of medicaments.

Background technology

Soybean isoflavones is the class secondary metabolite formed in the leguminous plants process of growth such as soybean, belongs to natural flavonoid material, has the typical structure of isoflavonoid, and its main component can be divided into Aglycons and the big class of Glucosides two. Soybean isoflavones has many-sided physiological functions such as anticancer, anti-cardiovascular disease, osteoporosis disease, as protective foods and medical health product Application and Development for many years. The research of livestock and poultry cultivation being shown in recent years, soybean isoflavones is for animal health, and the aspects such as control animal immune function is low, reproductivity decline, poor growth, efficiency of feed utilization are low all have unusual effect. Therefore, if the better soybean isoflavones product of result of use in livestock and poultry cultivation can be developed, using as a kind of novel green feed additive, there are wide market outlook.

Soybean germ be soybean at room temperature, soak the soybean after 36��48 hours with clear water, its plumule sprouts 1��2 millimeter, accounts for the 2.0%��2.5% of soybean gross weight. In the production in past, owing to beany flavor and the bitter taste of soybean germ self is often removed in processing, it is the Main By product that liquefaction and albumen generate, mostly it is not fully used, even lost as tankage, caused environmental pollution and the wasting of resources.

Scientific research shows, soybean germ is the position that isoflavone content is the highest, is about 2%, is 30��60 times of cotyledon, and its nutritive value is far longer than soybean, therefore becomes the raw material of a kind of excellent extraction soybean isoflavones just gradually. But, existing extractive technique is unsatisfactory. CN102838575A discloses the method for soybean isoflavones in a kind of expelling-expansion pretreatment assisted extraction soybean germ, its technique comprises multiple steps such as extruding puffing, enzymolysis, ultrasonic extraction, purification with macroreticular resin, freeze-drying, complicated operation, extraction cost height, and soybean isoflavones yield is less than 1.8%. CN101974577A provides the production technique of another kind of novel extraction isoflavone genin, although its finished product obtains the isoflavone genin of content about 40%, but leaching process comprises 8 key steps, there is the problem such as complicated operation, cost height equally. How the extracting method that a kind of operation steps is simple, receipts rate is high is provided, becomes a problem demanding prompt solution.

Summary of the invention

It is an object of the invention to provide a kind of soybean isoflavone and extracting method thereof.

The present invention provides a kind of soybean isoflavone, and it comprises the component of following weight per-cent:

Daidzin 1.8%��10%, glycitin 0.1%��1%, Genistoside 0.8%��8%, daidzein 2.1%��12%, glycitein 0.1%��1%, genistein 0.2%��2%.

Preferably, described soybean isoflavone comprises the component of following weight per-cent: daidzin 1.8%��5%, glycitin 0.1%��0.26%, Genistoside 0.8%��2.5%, daidzein 2.1%��5.4%, glycitein 0.1%��0.33%, genistein 0.2%��0.56%.

The present invention provides the extracting method of a kind of described soybean isoflavone, comprises following step:

A, being placed in multi-function extractor by soybean germ, the ratio adding 4L acetone in every kg soybean germ adds the mixing solutions of acetone and water, and wherein the ratio of acetone and water is 3:1 (v/v);

B, it is warming up to 65��75 DEG C, soaks 1 hour;

C, maintaining the temperature at 65��75 DEG C, extract 3.5 hours continuously, entering flow quantity is 1100L/h, and going out flow quantity is 1500L/h, collects extract;

D, by extract obtained for step c concentrated, spraying dry, to obtain final product.

Further, in described extracting method, b step is warming up to 70 DEG C; Step c maintains the temperature at 70 DEG C; Step d extract is concentrated to density be 1.3g/ml.

The present invention provides the purposes of described soybean isoflavone in the fodder additives of preparation raising Stock genetics and breeding.

Further, described fodder additives is the fodder additives improving cub production performance.

Further, described fodder additives is the fodder additives improving weanling pig production performance.

The present invention provides a kind of soybean isoflavone. Effect experimental shows, after adding soybean isoflavone of the present invention to piglet of feeding in feed, piglet production performance shows obvious raising. Compared with existing soybean isoflavones product, extract of the present invention has significant advantage, and under condition of feeding on an equal basis, day weight gain, the average index such as daily ingestion amount, feedstuff-meat ratio are all better than the U.S. power shield in commercially available prod, have good market outlook in livestock and poultry cultivation field. Present invention also offers the preparation method of described soybean isoflavone, its extraction process is simple, and extraction time is short, solvent for use energy rapid extraction special ingredient, and consumption is less, Extracting temperature is low, energy consumption is lower, reduces production cost to greatest extent, is conducive to scale operation.

Obviously, according to the foregoing of the present invention, according to ordinary technical knowledge and the customary means of this area, do not departing under the above-mentioned basic fundamental thought prerequisite of the present invention, it is also possible to make the amendment of other various ways, replacement or change.

The embodiment of form by the following examples, is described in further detail the foregoing of the present invention again. But this should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to following example. All technology realized based on foregoing of the present invention all belong to the scope of the present invention.

Embodiment

The raw material, the equipment that use in the specific embodiment of the invention are known product, obtain by buying commercially available prod.

The multi-function extractor below used purchased from Chun Lai Machinery Co., Ltd. of Changshu city of Jiangsu Province, model: CNC1692789350.

Isoflavone content measuring method

1, reagent and medicine

Methyl alcohol (chromatographically pure), acetonitrile (chromatographically pure), standard substance (Daidzin, soya bean yellow glucoside, Genistin, daidzein, glycitein, genistein).

2, plant and instrument

High performance liquid chromatograph; Visible UV-detector; N2000 chromatographic working station; Ultrasonic cleaner; Ten thousand/analytical balance and the conventional instrument of experiment.

3, chromatographic condition

Moving phase: water-acetonitrile (65:35);

Chromatographic column: C-18 (5 ��m, 150 �� 4.6mm);

Post temperature: 30 DEG C;

Flow velocity: 1.0mL/min;

Determined wavelength: 249nm;

Sampling volume: 20 �� L.

4, quantitative assay

The preparation of standard solution: accurately take appropriate Daidzin, the yellow glucoside of soya bean, Genistin, daidzein, glycitein or genistein standard substance respectively, ultrasonic dissolution is in methyl alcohol, and dilute and be settled to scale, shake even, make this every milliliter, standardized solution containing standard substance 50ug.

The preparation of need testing solution: accurately taking extract sample 0.5g (being accurate to 0.001g) of the present invention, ultrasonic dissolution, in 100ml methyl alcohol, is diluted to suitable concentration, shakes even. Accurate standard solution and each 20 �� L of need testing solution drawing 0.45um filter membrane respectively, injection liquid chromatography, measures.

5, the calculating of measurement result

In sample, isoflavone content is calculated as follows:

$\mathrm{\ω}i=\frac{Pi\×V\×n\×Cst}{Pst\×m}$

In formula:

The content of soybean isoflavones (Daidzin, the yellow glucoside of soya bean, Genistin, daidzein, glycitein or genistein) in �� i sample, mg/kg;

M sample mass, g;

Pi sample solution peak area value;

The final constant volume of V sample, mL;

N sample extension rate;

Cst concentration of standard solution, �� g/mL;

Pst standardized solution peak area mean value.

The measurement result arithmetical av of replicate(determination) represents, retains 3 position effective digitals.

The preparation of embodiment 1 extract of the present invention

By soybean germ 500kg, adding in the multi-function extractor of 3 tons, add the acetone of 2000 liters and the mixing solutions of water, wherein the ratio of acetone and water is 3:1 (v/v);

It is warming up to 65 DEG C, soaks 1 hour;

Extracting 3.5 hours continuously, maintain the temperature at 65 DEG C, entering flow quantity is 1100 ls/h, and going out flow quantity is 1500 ls/h;

To concentrate to density be 1.3g/mL by extract obtained, and spraying dry, obtains soybean extraction 97.6kg.

The receipts rate of products obtained therefrom is 90.5%, wherein the mass percent of each index composition is: Daidzin 1.8%, soya bean yellow glucoside 0.1%, Genistin 0.8%, daidzein 2.1%, glycitein 0.1%, genistein 0.2%, soybean isoflavones total content is 5.1%.

The preparation of embodiment 2 extract of the present invention

By soybean germ 500kg, adding in the multi-function extractor of 3 tons, add the acetone of 2000 liters and the mixing solutions of water, wherein the ratio of acetone and water is 3:1 (v/v);

It is warming up to 70 DEG C, soaks 1 hour;

Extracting 3.5 hours continuously, maintain the temperature at 70 DEG C, entering flow quantity is 1100 ls/h, and going out flow quantity is 1500 ls/h;

To concentrate to density be 1.3g/mL by extract obtained, and spraying dry, obtains soybean extraction 54.5kg.

The receipts rate of products obtained therefrom is 93.2%, wherein the mass percent of each index composition is: Daidzin 3.5%, soya bean yellow glucoside 0.18%, Genistin 1.56%, daidzein 3.47%, glycitein 0.26%, genistein 0.43%, soybean isoflavones total content is 9.4%.

The preparation of embodiment 3 extract of the present invention

By soybean germ 500kg, adding in the multi-function extractor of 3 tons, add the acetone of 2000 liters and the mixing solutions of water, wherein the ratio of acetone and water is 3:1 (v/v);

It is warming up to 75 DEG C, soaks 1 hour;

Extracting 3.5 hours continuously, maintain the temperature at 75 DEG C, entering flow quantity is 1100 ls/h, and going out flow quantity is 1500 ls/h;

To concentrate to density be 1.3g/mL by extract obtained, and spraying dry, obtains soybean extraction 37.5kg.

The receipts rate of products obtained therefrom is 95.7%, wherein the mass percent of each index composition is: Daidzin 5%, soya bean yellow glucoside 0.26%, Genistin 2.5%, daidzein 5.4%, glycitein 0.33%, genistein 0.56%, soybean isoflavones total content is 14.05%.

The useful effect of the present invention is proved below by way of effect experimental.

Experimental example 1 extract of the present invention is on the impact of weanling pig production performance

Test method: select 180 21 days weanling pigs, it is assigned randomly to 5 treatment group, it is respectively base set (CON), the existing soybean isoflavones of base set+200mg/kg (commercially available prod title: U.S. power shield, composition: 4 ', 7-dihydroxy isoflavone, No. 0 group), base set+200mg/kg embodiment 1 soybean isoflavones (No. 1 group), base set+200mg/kg embodiment 2 soybean isoflavones (No. 2 groups), base set+200mg/kg embodiment 3 soybean isoflavones (No. 3 groups). Base set (CON) is fed corn-soybean meal diet, and corresponding soybean isoflavones is added to piglet of feeding in the feed identical with base set by 0��No. 3 group. 28 days trial periods. Inspection target: day weight gain, average daily ingestion amount, feedstuff-meat ratio.

Table 1 extract of the present invention is on the impact of weanling pig production performance

Experimental result: being added to by soybean isoflavone of the present invention and feed piglet in feed after 14 days, piglet production performance shows obvious raising. Compared to the base set of basal feed of only feeding, piglet day weight gain is increased to more than 231g by 179g, and average daily ingestion amount is increased to more than 321g by 256g, and feedstuff-meat ratio is reduced to less than 1.39 by 1.42; Especially the soybean isoflavone prepared according to embodiment 3, can make piglet day weight gain be increased to 249g, and average daily ingestion amount is increased to 334g, and feedstuff-meat ratio is reduced to 1.35, and effect is more superior. Compared with existing soybean isoflavones product, extract of the present invention also has significant advantage, and under condition of feeding on an equal basis, day weight gain, the average index such as daily ingestion amount, feedstuff-meat ratio are all better than the U.S. power shield in commercially available prod. Continuation extract of the present invention is fed after piglet to 28 day, and day weight gain can be increased to 357g, and average daily ingestion amount is increased to 486g, and feedstuff-meat ratio is reduced to 1.37, has significant effect in raising piglet production performance.

More than experiment shows, soybean isoflavone of the present invention uses as fodder additives, can significantly improve Stock genetics and breeding, and effect is better than commercially available soybean isoflavones product, has good market outlook in livestock and poultry cultivation field.

Claims (7)

1. a soybean isoflavone, is characterized in that: it comprises the component of following weight per-cent: daidzin 1.8%��10%, glycitin 0.1%��1%, Genistoside 0.8%��8%, daidzein 2.1%��12%, glycitein 0.1%��1%, genistein 0.2%��2%.

2. soybean isoflavone as claimed in claim 1, is characterized in that: it comprises the component of following weight per-cent: daidzin 1.8%��5%, glycitin 0.1%��0.26%, Genistoside 0.8%��2.5%, daidzein 2.1%��5.4%, glycitein 0.1%��0.33%, genistein 0.2%��0.56%.

3. an extracting method for soybean isoflavone described in claim 1 or 2, is characterized in that: comprise following step:

A, being placed in multi-function extractor by soybean germ, the ratio adding 4L acetone in every kg soybean germ adds the mixing solutions of acetone and water, and wherein the ratio of acetone and water is 3:1 (v/v);

B, it is warming up to 65��75 DEG C, soaks 1 hour;

C, maintaining the temperature at 65��75 DEG C, extract 3.5 hours continuously, entering flow quantity is 1100L/h, and going out flow quantity is 1500L/h, collects extract;

D, by extract obtained for step c concentrated, spraying dry, to obtain final product.

4. the extracting method of soybean isoflavone as claimed in claim 3, is characterized in that: b step is warming up to 70 DEG C; Step c maintains the temperature at 70 DEG C; Step d extract is concentrated to density be 1.3g/ml.

5. soybean isoflavone described in claim 1 or 2 improves the purposes in the fodder additives of Stock genetics and breeding in preparation.

6. purposes as claimed in claim 5, is characterized in that: described fodder additives is the fodder additives improving cub production performance.

7. purposes as claimed in claim 6, is characterized in that: described fodder additives is the fodder additives improving weanling pig production performance.