CN104987430A - Method for preparing cicer arietinum peel polysaccharide with antioxidant activity - Google Patents
Method for preparing cicer arietinum peel polysaccharide with antioxidant activity Download PDFInfo
- Publication number
- CN104987430A CN104987430A CN201510449952.6A CN201510449952A CN104987430A CN 104987430 A CN104987430 A CN 104987430A CN 201510449952 A CN201510449952 A CN 201510449952A CN 104987430 A CN104987430 A CN 104987430A
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- cicer arietinum
- peel
- olecranon
- beancurd
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for preparing cicer arietinum peel polysaccharide with antioxidant activity by hot water extraction. The method comprises: separating cicer arietinum peel; drying cicer arietinum peel; crushing cicer arietinum peel; carrying out ethanol impurity removal; carrying out hot water extraction; centrifuging the cicer arietinum peel to obtain liquid supernatant; carrying out decompression concentration on the liquid supernatant; carrying out ethanol precipitation on the liquid supernatant; centrifuging the liquid supernatant to obtain precipitates; sequentially washing the precipitates by ethanol and acetone; cooling and drying the precipitates to constant weight, thereby obtaining the cicer arietinum peel polysaccharide. A method of removing DPPH free radicals in vitro is adopted to analyze the antioxidant activity of the cicer arietinum peel polysaccharide, and the cicer arietinum peel polysaccharide is found to have remarkable ability of eliminating free radicals. The preparation method for the cicer arietinum peel polysaccharide disclosed by the invention has the advantages such as a gentle extracting process, low cost, a complete polysaccharide structure, no pollution on environment and the like; and the prepared cicer arietinum peel polysaccharide has a broad applicationprospect in food industry and pharmaceutical industry because of remarkable antioxidant activity,
Description
Technical field
The present invention relates to the technical field extracting Related Component from pulse family vetch race garbanzo platymiscium skin of beancurd, be specifically related to a kind ofly prepare the method with anti-oxidant activity olecranon skin of beancurd polysaccharide.
Background technology
Garbanzo (Cicer arietinum) belongs to pulse family vetch race olecranon Macroptilium, annual or per nnial herb, likeness in form olecranon.Another name peach beans, chicken pea, Uighur claims " Nuo Hu carries ".The cultivation history of garbanzo is long, and it is western to originate from sub-state, area, the Near East, and being mainly distributed in warm and comparatively arid area, is one of leguminous plants that cultivated area is wider in the world.High 1-2 rice, stem is upright, and multi-branched, by white glandular hairs.Stipule is lobate, or tool 3-5 irregular sawtooth or lower edge have thin sawtooth.Garbanzo is a kind of edible beans of high nutritive value, is rich in various plants amino acid, protein, VITAMIN, mineral substance, the compositions such as robust fibre.In addition also VITAMIN B4, choline, inositol, starch, glucose, sucrose etc. are contained in seed.Garbanzo contains ten multiple amino acids, and wherein human body 8 kinds must all possess by amino acid.Garbanzo has balance diet, hypoglycemic, hypotensive, reducing blood-fat, raising children ' s intelligence development, enhances skeletal growth and the multiple eating such as to delay senility is worth and nourishing function.But it is up to now, rarely seen in report to the research of olecranon skin of beancurd polysaccharide both at home and abroad.
Summary of the invention
A kind of method that the present invention aims to provide low cost, pollution-free, efficient preparation has anti-oxidant activity olecranon skin of beancurd polysaccharide, realizes concrete scheme of the present invention as follows:
(1) garbanzo clear water is soaked 2min, manual peeling.
(2) olecranon skin of beancurd 63.5 DEG C oven dry step (1) obtained, pulverizes, and crosses 60 mesh sieves, obtains evengranular crushed material;
(3) by the crushed material that step (2) is obtained, soak removal of impurities twice with 80% ethanol 80 DEG C, each 1h, centrifugal (4000rpm, 15min) abandons supernatant, and gained residue dries naturally;
(4) step (3) gained residue is pressed liquid ratio 10-30mL/g, add deionized water and carry out hot water extraction, Extracting temperature 60-100 DEG C, time 1-5h, extraction time 1-4 time, obtains extracting solution;
(5) step (4) gained extracting solution centrifugal (4000rpm, 15min) is got supernatant liquor;
(6) by step (5) gained supernatant liquor under 50 DEG C of conditions, be evaporated to suitable volume, add 4 times of dehydrated alcohols in concentrated solution, 4 DEG C of precipitates overnight, centrifugal (4000rpm, 15min) gets precipitation;
(7) step (6) gained throw out is used ethanol and washing with acetone successively, lyophilize, to constant weight, is olecranon skin of beancurd polysaccharide;
(8) step (7) gained polysaccharide deionized water is mixed with a series of strength solution respectively, measures it to DPPH radical scavenging activity.
The olecranon skin of beancurd polysaccharide of preparation is pale yellow powder, and this polysaccharide presents dose-dependence to DPPH free radical scavenging activity, and when polysaccharide concentration is 2.5mg/mL, clearance rate is 54.56%.
2, a kind of hot water extraction according to patent requirements 1 has the method for the olecranon skin of beancurd polysaccharide of anti-oxidant activity, it is characterized in that: the liquid ratio 24mL/g of described step (4), Extracting temperature 99 DEG C, extraction time 3h, extraction time 2 times.
The olecranon skin of beancurd polysaccharide of preparation is pale yellow powder, and this polysaccharide presents dose-dependence to DPPH free radical scavenging activity, and when polysaccharide concentration is 2.5mg/mL, clearance rate is 54.56%.
Accompanying drawing explanation
Fig. 1 Extracting temperature (DEG C) and liquid ratio (mL/g) are on the impact of olecranon skin of beancurd polysaccharide extract rate
Fig. 2 extraction time (h) and liquid ratio (mL/g) are on the impact of olecranon skin of beancurd polysaccharide extract rate
Fig. 3 Extracting temperature (DEG C) and extraction time (h) are on the impact of olecranon skin of beancurd polysaccharide extract rate
Fig. 4 olecranon skin of beancurd polysaccharide is to DPPH free radical scavenging effect
Embodiment
Below by embodiment, the invention will be further described.
Embodiment 1:
(1) garbanzo clear water is soaked 2min, manual peeling.
(2) olecranon skin of beancurd 63.5 DEG C oven dry step (1) obtained, pulverizes, and crosses 60 mesh sieves, obtains evengranular crushed material;
(3) by the crushed material that step (2) is obtained, soak removal of impurities twice with 80% ethanol 80 DEG C, each 1h, centrifugal (4000rpm, 15min) abandons supernatant, and gained residue dries naturally;
(4) step (3) gained residue is pressed liquid ratio 10-30mL/g, add deionized water and carry out hot water extraction, Extracting temperature 60-100 DEG C, time 1-5h, extraction time 1-4 time, obtains extracting solution;
(5) step (4) gained extracting solution centrifugal (4000rpm, 15min) is got supernatant liquor;
(6) by step (5) gained supernatant liquor under 50 DEG C of conditions, be evaporated to suitable volume, add 4 times of dehydrated alcohols in concentrated solution, 4 DEG C of precipitates overnight, centrifugal (4000rpm, 15min) gets precipitation;
(7) step (6) gained throw out is used ethanol and washing with acetone successively, lyophilize, to constant weight, is olecranon skin of beancurd polysaccharide;
(8) step (7) gained polysaccharide deionized water is mixed with a series of strength solution respectively, measures it to DPPH radical scavenging activity.
The olecranon skin of beancurd polysaccharide of preparation is pale yellow powder, and this polysaccharide presents dose-dependence to DPPH free radical scavenging activity, and when polysaccharide concentration is 2.5mg/mL, clearance rate is 54.56%.
Olecranon skin of beancurd polysaccharide extract rate presses formulae discovery below:
Polysaccharide extract rate (%)=[Crude polysaccharides quality (g)/removal of impurities raw materials quality (g)] × 100%
Clearance rate (%)=[A0-(A1-A2)]/A0 × 100%
Wherein Ao is the light absorption value of control experiment (water replacement sample solution);
A1 is the light absorption value of sample experiments;
A2 is the light absorption value of sample interference experiment (dehydrated alcohol replaces DPPH solution).
Embodiment 2:
Preparation has the optimization for extracting condition experiment of anti-oxidant activity olecranon skin of beancurd polysaccharide
(1) preparation has the experiment of single factor of the optimization for extracting condition of anti-oxidant activity olecranon skin of beancurd polysaccharide
1. liquid ratio is on the impact of olecranon skin of beancurd polysaccharide extract rate
Liquid ratio is set to 10,15,20,25 and 30mL/g five gradients, and Extracting temperature, extraction time and extraction time are respectively 90 DEG C, 3h and 2.The polysaccharide extract rate of different liquid ratio is in table 1:
As can be seen from Table 1: after liquid ratio reaches 20mL/g, extraction yield substantially no longer rises, therefore to choose 20mL/g be response surface central point.
2. Extracting temperature is on the impact of olecranon skin of beancurd polysaccharide extract rate
Extracting temperature arranges 60,70,80,90 and 100 DEG C of five gradients, and extraction time, liquid ratio and extraction time are respectively 3h, 20mL/g and 2 times.The polysaccharide extract rate of different Extracting temperature is in table 2:
As can be seen from Table 2: when 100 DEG C, although extraction yield is high, in order to energy-conservation consideration, choose 90 DEG C for response surface central point.
3. extraction time is on the impact of olecranon skin of beancurd polysaccharide extract rate
Extraction time is set to 1,2,3,4 and 5h five gradients, and Extracting temperature, liquid ratio and extraction time are respectively 90 DEG C, 20mL/g and 2.The polysaccharide extract rate of different extraction time is in table 3:
As can be seen from Table 3: after extraction time 2h, extraction yield substantially no longer rises, and after 3h, extraction yield slowly declines, therefore to choose 2h be response surface central point.
4. extraction time is on the impact of olecranon skin of beancurd polysaccharide extract rate
Extraction time is set to 1,2,3 and 4 four gradient, and Extracting temperature, extraction time and liquid ratio are respectively 90 DEG C, 3h and 20mL/g.The polysaccharide extract rate of different extraction time is in table 4:
As can be seen from Table 4: extraction time more than 2 times after, extraction yield rises slowly, therefore during response surface analysis, extraction time is set to 2 times.
(2) preparation has the response surface analysis experiment of the optimization for extracting condition of anti-oxidant activity olecranon skin of beancurd polysaccharide
Preparation has the response surface analysis experimental result of the optimization for extracting condition of anti-oxidant activity olecranon skin of beancurd polysaccharide as table 5:
According to table 5 result, obtaining multiple regression equation with Design Expert8.0.7 software analysis is: Y=0.0798X
1+ 1.14465X
2-0.1555X
3+ 0.00355X
1x
2-0.0015X
1x
3+ 0.02775X
2x
3-0.00897X
1 2-0.0065925X
2 2-0.46425X
3 2-51.79825
(wherein Y is extraction yield, X
1for solid-liquid ratio, X
2for Extracting temperature, X
3for extraction time)
Obtaining optimum extraction condition according to Equation for Calculating is: liquid ratio 24.34mL/g, Extracting temperature 99.34 DEG C, extraction time 2.84h.With this understanding, the extraction yield of olecranon skin of beancurd polysaccharide is 5.81%.Consider the accessibility of actually operating, finally show that olecranon skin of beancurd polysaccharide optimum extraction process is: Extracting temperature 99 DEG C, liquid ratio is 24mL/g, and extraction time is 3h, and extraction time is 2 times.
Be only of the present invention in sum and goodly execute example, be not used for limiting practical range of the present invention.Namely all equivalence changes done according to the content of the present patent application the scope of the claims all should be technology category of the present invention with modification.
Claims (2)
1. hot water extraction's preparation has a method for the olecranon skin of beancurd polysaccharide of anti-oxidant activity, it is characterized in that comprising following operation steps:
(1) garbanzo clear water is soaked 2min, manual peeling.
(2) olecranon skin of beancurd 63.5 DEG C oven dry step (1) obtained, pulverizes, and crosses 60 mesh sieves, obtains evengranular crushed material;
(3) by the crushed material that step (2) is obtained, soak removal of impurities twice with 80% ethanol 80 DEG C, each 1h, centrifugal (4000rpm, 15min) abandons supernatant, and gained residue dries naturally;
(4) step (3) gained residue is pressed liquid ratio 10-30mL/g, add deionized water and carry out hot water extraction, Extracting temperature 60-100 DEG C, time 1-5h, extraction time 1-4 time, obtains extracting solution;
(5) step (4) gained extracting solution centrifugal (4000rpm, 15min) is got supernatant liquor;
(6) by step (5) gained supernatant liquor under 50 DEG C of conditions, be evaporated to suitable volume, add 4 times of dehydrated alcohols in concentrated solution, 4 DEG C of precipitates overnight, centrifugal (4000rpm, 15min) gets precipitation;
(7) step (6) gained throw out is used ethanol and washing with acetone successively, lyophilize, to constant weight, is olecranon skin of beancurd polysaccharide;
(8) step (7) gained polysaccharide deionized water is mixed with a series of strength solution respectively, measures it to DPPH radical scavenging activity.
The olecranon skin of beancurd polysaccharide of preparation is pale yellow powder, and this polysaccharide presents dose-dependence to DPPH free radical scavenging activity, and when polysaccharide concentration is 2.5mg/mL, clearance rate is 54.56%.
2. a kind of hot water extraction according to patent requirements 1 has the method for the olecranon skin of beancurd polysaccharide of anti-oxidant activity, it is characterized in that: the liquid ratio 24mL/g of described step (4), Extracting temperature 99 DEG C, extraction time 3h, extraction time 2 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510449952.6A CN104987430A (en) | 2015-07-28 | 2015-07-28 | Method for preparing cicer arietinum peel polysaccharide with antioxidant activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510449952.6A CN104987430A (en) | 2015-07-28 | 2015-07-28 | Method for preparing cicer arietinum peel polysaccharide with antioxidant activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104987430A true CN104987430A (en) | 2015-10-21 |
Family
ID=54299342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510449952.6A Pending CN104987430A (en) | 2015-07-28 | 2015-07-28 | Method for preparing cicer arietinum peel polysaccharide with antioxidant activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104987430A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108530553A (en) * | 2018-04-28 | 2018-09-14 | 中国农业科学院作物科学研究所 | A kind of preparation method of chick-pea neutral polysaccharide |
| CN113875983A (en) * | 2020-07-01 | 2022-01-04 | 新疆师范大学 | Preparation method of chickpea polysaccharide capsules |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2038891A1 (en) * | 1991-05-16 | 1993-08-01 | Intercontinental Grup S A | Prodn. of chick-peas based aperitif |
| CN102228201A (en) * | 2011-06-22 | 2011-11-02 | 中国科学院新疆理化技术研究所 | Chickpea polysaccharide nutrition powder and preparation method thereof |
-
2015
- 2015-07-28 CN CN201510449952.6A patent/CN104987430A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2038891A1 (en) * | 1991-05-16 | 1993-08-01 | Intercontinental Grup S A | Prodn. of chick-peas based aperitif |
| CN102228201A (en) * | 2011-06-22 | 2011-11-02 | 中国科学院新疆理化技术研究所 | Chickpea polysaccharide nutrition powder and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| VANIA URIAS-ORONA等: ""Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin"", 《MOLECULES》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108530553A (en) * | 2018-04-28 | 2018-09-14 | 中国农业科学院作物科学研究所 | A kind of preparation method of chick-pea neutral polysaccharide |
| CN108530553B (en) * | 2018-04-28 | 2020-12-25 | 中国农业科学院作物科学研究所 | Preparation method of chickpea neutral polysaccharides CWP2-1 and CWP2-2 |
| CN113875983A (en) * | 2020-07-01 | 2022-01-04 | 新疆师范大学 | Preparation method of chickpea polysaccharide capsules |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101607218A (en) | Corn steam explosion separates and endosperm multi-component co-production utilizes technology | |
| CN104544137A (en) | Novel method for preparing wheat bran dietary fibers by taking wheat bran as raw material | |
| CN105602700A (en) | Preparation method of cold-pressed peony seed oil with high oil yield | |
| CN103290081A (en) | Extraction method of rice bran active polysaccharide | |
| CN108813615B (en) | Preparation method of mung bean extract with antioxidant activity | |
| CN107541332B (en) | Moringa oleifera oil and preparation method thereof | |
| CN104987430A (en) | Method for preparing cicer arietinum peel polysaccharide with antioxidant activity | |
| CN110903677A (en) | Method for simultaneously preparing gardenia yellow pigment and blue pigment | |
| CN108359571B (en) | Production process of white tea fruit distilled liquor | |
| CN104726197A (en) | Method for extracting tobacco seed oil from aromatic tobacco waste tobacco seed | |
| CN101558817B (en) | Method for producing protein concentrate by high temperature peanut meal | |
| CN106432387A (en) | Combined extraction method for rutin and polysaccharide in buckwheat seeds | |
| CN103351940B (en) | Manufacturing technology of camellia sinensis seed oil through biological fermentation method | |
| CN103289809B (en) | Method for preparing walnut seed kernel oil by adopting brine enzymatic method | |
| CN104543373A (en) | Method for synchronously extracting peanut oil and performing high-value utilization to cakes | |
| CN109043117A (en) | A kind of acidity Queensland nut glycoprotein and its production method | |
| CN103652308A (en) | Industrial preparation method of high-protein grape seed dregs | |
| CN110540561A (en) | A method for extracting geniposide from fructus Gardeniae | |
| CN111534366B (en) | Method for preparing oat bran oil rich in polyphenol by taking naked oat bran as raw material | |
| CN102669403A (en) | Process for extracting rice slag protein by organic solvent washing method | |
| CN109954009A (en) | A kind of joint production process extracting SOD and general flavone from leaf of Moringa | |
| CN110643423A (en) | Low-temperature oil pressing process applied to cyperus esculentus | |
| CN106820167A (en) | A kind of preparation method of wild pepper seed soluble dietary fiber | |
| CN110804493A (en) | Extraction process of clausena lansium volatile oil | |
| CN104725524A (en) | Method for extracting tobacco polysaccharides from waste tobacco stems in farmlands |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151021 |
|
| WD01 | Invention patent application deemed withdrawn after publication |