CN110540561A - A method for extracting geniposide from fructus Gardeniae - Google Patents
A method for extracting geniposide from fructus Gardeniae Download PDFInfo
- Publication number
- CN110540561A CN110540561A CN201810531226.2A CN201810531226A CN110540561A CN 110540561 A CN110540561 A CN 110540561A CN 201810531226 A CN201810531226 A CN 201810531226A CN 110540561 A CN110540561 A CN 110540561A
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- coarse powder
- geniposide
- ethanol
- gardenia
- eluting
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
the invention discloses a preparation method for extracting geniposide from gardenia, which is characterized by comprising the following steps: 1) crushing the dried gardenia raw material, sieving the crushed gardenia raw material by a sieve with 20-60 meshes, and collecting coarse powder for later use; 2) enzymolysis: weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 3-5, 50-70% of ethanol, adding 0.1-0.3% of biological enzyme by weight of the coarse powder, and soaking and performing enzymolysis for 1-2 hours at the pH value of 5 and the temperature of 40-50 ℃; 3) leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 90-120min, and concentrating; 4) passing the obtained concentrated solution through a macroporous adsorption resin column, eluting with 2-5 times of column volume of distilled water, eluting with 60-80% ethanol, and collecting ethanol eluate; 5) recovering the reagent, and vacuum drying to obtain the geniposide. The geniposide prepared by the invention has high purity and good quality.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a preparation method of geniposide.
background
Cape jasmine fruit, another name: gardenia jasminoides Ellis, Gardenia jasminoides Ellis and Cinobufagin are fruits of Gardenia jasminoides Ellis of Rubiaceae. The fruit of gardenia is a traditional Chinese medicine, belongs to the first batch of medical and edible dual-purpose resources issued by the Ministry of health, and has the functions of protecting liver, benefiting gallbladder, reducing blood pressure, calming, stopping bleeding, reducing swelling and the like. Is used for treating icteric hepatitis, sprain, contusion, hypertension, diabetes and other diseases in traditional Chinese medicine clinic. Contains crocetin glycoside group, and can be used as yellow dye.
The geniposide is extracted and refined from dried mature fruit of Gardenia jasminoides Ellis of Rubiaceae by high-tech production process. The geniposide is iridoid glycoside compound, namely geniposide, and geniposide. The geniposide has multiple purposes, can be fermented under different conditions, can be made into natural edible colorants gardenia blue and gardenia red, and is also a raw material medicine for treating cardiovascular and cerebrovascular diseases, liver and gall diseases and diabetes.
Disclosure of Invention
The invention aims to provide an extraction method of geniposide, and the geniposide has wide medicinal value.
The invention is completed by the following technical scheme, and the specific content comprises the following steps: method for extracting geniposide
1) Crushing the dried gardenia raw material, sieving the crushed gardenia raw material by a sieve with 20-60 meshes, and collecting coarse powder for later use;
2) Enzymolysis: weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 3-5, 50-70% of ethanol, adding 0.1-0.3% of biological enzyme by weight of the coarse powder, and soaking and performing enzymolysis for 1-2 hours at the pH value of 5 and the temperature of 40-50 ℃;
3) leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 90-120min, and concentrating;
4) Passing the obtained concentrated solution through a macroporous adsorption resin column, eluting with 2-5 times of column volume of distilled water, eluting with 60-80% ethanol, and collecting ethanol eluate;
5) Recovering the reagent, and vacuum drying to obtain the geniposide.
The biological enzyme in the step 2) is one or a mixture of more than two of pectinase, cellulase and hemicellulase.
the macroporous adsorption resin in the step 5) is one of X-5, LSA-21 and WLD.
the present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.
the specific implementation mode is as follows:
example 1:
Pulverizing dried fructus Gardeniae raw material, sieving with 20 mesh sieve, and collecting coarse powder; weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 3. soaking in 50% ethanol, adding 0.1% weight of biological enzyme pectinase and cellulase, and performing enzymolysis at 40 deg.C and pH 5 for 1 hr; leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 90min, and concentrating; passing the obtained concentrated solution through macroporous adsorbent resin column X-5, eluting with 2 times column volume of distilled water, eluting with 60% ethanol, and collecting ethanol eluate; recovering the reagent, and vacuum drying to obtain the geniposide.
example 2:
pulverizing dried fructus Gardeniae raw material, sieving with 60 mesh sieve, and collecting coarse powder; weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 5. Soaking in 70% ethanol, adding 0.3% weight of biological enzymes such as cellulase and hemicellulase, and performing enzymolysis at 50 deg.C and pH of 5 for 2 hr; leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 120min, and concentrating; loading the obtained concentrated solution onto macroporous adsorbent resin column LSA-21, eluting with 5 times of column volume of distilled water, eluting with 80% ethanol, and collecting ethanol eluate; recovering the reagent, and vacuum drying to obtain the geniposide.
Example 3:
pulverizing dried fructus Gardeniae raw material, sieving with 50 mesh sieve, and collecting coarse powder; weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 4. soaking in 60% ethanol, adding 0.2% weight of biological enzymes such as cellulase and hemicellulase, and performing enzymolysis at 50 deg.C and pH of 5 for 2 hr; leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 100min, and concentrating; loading the obtained concentrated solution onto a macroporous adsorbent resin column WLD, eluting with 4 times of column volume of distilled water, eluting with 70% ethanol, and collecting ethanol eluate; recovering the reagent, and vacuum drying to obtain the geniposide.
Claims (3)
1. A preparation method for extracting geniposide from gardenia is characterized by comprising the following steps:
1) crushing the dried gardenia raw material, sieving the crushed gardenia raw material by a sieve with 20-60 meshes, and collecting coarse powder for later use;
2) Enzymolysis: weighing a certain amount of coarse powder, and adding the coarse powder into the mixture according to a material-liquid ratio of 1: 3-5, 50-70% of ethanol, adding 0.1-0.3% of biological enzyme by weight of the coarse powder, and soaking and performing enzymolysis for 1-2 hours at the pH value of 5 and the temperature of 40-50 ℃;
3) Leaching the enzymatic hydrolysate in a constant temperature water bath at 70 deg.C for 90-120min, and concentrating;
4) Passing the obtained concentrated solution through a macroporous adsorption resin column, eluting with 2-5 times of column volume of distilled water, eluting with 60-80% ethanol, and collecting ethanol eluate;
5) Recovering the reagent, and vacuum drying to obtain the geniposide.
2. The method according to claim 1, wherein the bio-enzyme in step 2) is one or a mixture of two or more of pectinase, cellulase and hemicellulase.
3. the method according to claim 1, wherein the macroporous adsorbent resin in step 5) is one of X-5, LSA-21, and WLD.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810531226.2A CN110540561A (en) | 2018-05-29 | 2018-05-29 | A method for extracting geniposide from fructus Gardeniae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810531226.2A CN110540561A (en) | 2018-05-29 | 2018-05-29 | A method for extracting geniposide from fructus Gardeniae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110540561A true CN110540561A (en) | 2019-12-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810531226.2A Pending CN110540561A (en) | 2018-05-29 | 2018-05-29 | A method for extracting geniposide from fructus Gardeniae |
Country Status (1)
| Country | Link |
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| CN (1) | CN110540561A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112251430A (en) * | 2020-10-14 | 2021-01-22 | 浙江农林大学 | Method for extracting gardenia blue pigment |
| CN113546117A (en) * | 2020-04-23 | 2021-10-26 | 百岳特生物技术（上海）有限公司 | Preparation method for improving geniposide content and application of regulating gene expression level |
-
2018
- 2018-05-29 CN CN201810531226.2A patent/CN110540561A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113546117A (en) * | 2020-04-23 | 2021-10-26 | 百岳特生物技术（上海）有限公司 | Preparation method for improving geniposide content and application of regulating gene expression level |
| CN112251430A (en) * | 2020-10-14 | 2021-01-22 | 浙江农林大学 | Method for extracting gardenia blue pigment |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20191206 |
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| WD01 | Invention patent application deemed withdrawn after publication |