CN106820167A - A kind of preparation method of wild pepper seed soluble dietary fiber - Google Patents
A kind of preparation method of wild pepper seed soluble dietary fiber Download PDFInfo
- Publication number
- CN106820167A CN106820167A CN201510878899.1A CN201510878899A CN106820167A CN 106820167 A CN106820167 A CN 106820167A CN 201510878899 A CN201510878899 A CN 201510878899A CN 106820167 A CN106820167 A CN 106820167A
- Authority
- CN
- China
- Prior art keywords
- pepper seed
- wild pepper
- dietary fiber
- soluble dietary
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000302909 Piper aduncum Species 0.000 title claims abstract description 32
- 235000016761 Piper aduncum Nutrition 0.000 title claims abstract description 32
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 229940088598 enzyme Drugs 0.000 claims abstract description 19
- 238000004945 emulsification Methods 0.000 claims abstract description 14
- 238000005238 degreasing Methods 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000012869 ethanol precipitation Methods 0.000 claims abstract description 6
- 108010059892 Cellulase Proteins 0.000 claims abstract description 5
- 229940106157 cellulase Drugs 0.000 claims abstract description 5
- 239000003208 petroleum Substances 0.000 claims abstract description 5
- 239000013049 sediment Substances 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 13
- 230000000694 effects Effects 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 238000007605 air drying Methods 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 238000001291 vacuum drying Methods 0.000 claims description 2
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 239000000284 extract Substances 0.000 abstract description 3
- 238000005119 centrifugation Methods 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract description 2
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 abstract 1
- 244000089698 Zanthoxylum simulans Species 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 108010084185 Cellulases Proteins 0.000 description 3
- 102000005575 Cellulases Human genes 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 235000013376 functional food Nutrition 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 241000949456 Zanthoxylum Species 0.000 description 1
- 238000009098 adjuvant therapy Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000012259 ether extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000036564 melanin content Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation method of wild pepper seed soluble dietary fiber, including step：(1) wild pepper seed ungrease treatment：Ungrease treatment is carried out to wild pepper seed by extractant of petroleum ether, dried, crushed；(2) enzyme process prepares wild pepper seed soluble dietary fiber：Degreasing wild pepper seed powder, adds suitable quantity of water, emulsification pretreatment, using cellulase and basic protein, centrifugation, filtering, then with ethanol precipitation, dry sediment obtains soluble dietary fiber.The present invention extracts soluble dietary fiber yield to wild pepper seed up to 90%, improves the comprehensive utilization value of wild pepper seed, to wild pepper seed resource discarded object comprehensive development and utilization, extracts low-cost.
Description
Technical field
The invention belongs to food technology of preparing neck, more particularly to it is a kind of preparation method of wild pepper seed soluble dietary fiber.
Background technology：
The perennial shrub of Zanthoxylum Rutaceae or dungarunga, are one of traditional " eight big flavouring ", in existing more than the 2000 years cultivation history of China.China is that Chinese prickly ash produces the first big country, wild pepper seed is the Main By product in the production of Chinese prickly ash pericarp, it is more rich in melanin content in grease (27.1%), protein (18.7%) and crude fibre (30.23%), and kind skin.In addition to sub-fraction is left as seed seedling-raising, most of wild pepper seed is burned off or as fertilizer, or even is taken as waste material discarding.At present, the research on utilization to wild pepper seed is concentrated mainly on the extraction and application of Fructus Zanthoxyli oil, and on coarse-fibred exploitation in wild pepper seed, there is not been reported.
Different according to dissolubility, dietary fiber is divided into insoluble diedairy fiber (IDF, insoluble dietary fiber) and soluble dietary fiber (SDF, soluble dietary fiber).SDF can reduce the content of LDL-C in blood plasma, promote beneficial flora growth and breeding in enteron aisle, can be used as the functional food of the diseases such as adjuvant therapy of cardiovascular.Additionally, dietary fiber can also be added in many food as a kind of functional food ingredient, to improve nutritive value of food, so as to promote the development of functional food.The present invention optimizes the method that enzyme process prepares soluble dietary fiber with the abundant wild pepper seed of crude fiber content as raw material.
The content of the invention
The purpose of the present invention is a kind of preparation method of wild pepper seed soluble dietary fiber.
The preparation method of the soluble dietary fiber that the present invention is provided, comprises the following steps：
1）The preparation of degreasing wild pepper seed：The wild pepper seed crushed is weighed, with petroleum ether as extractant, solid-liquid ratio 1g：10-15ml, 55 DEG C of temperature, the h of degreasing 2；Wild pepper seed after degreasing is placed in 60 DEG C of baking ovens and is dried, be crushed to 40 mesh standby；
2）The preparation of soluble dietary fiber：Degreasing wild pepper seed powder is weighed, 20-30 times of weighing body ponding, emulsification pretreatment is added；PH 4.5 is adjusted, 2.0% cellulase is added, 40-50 DEG C, 8-10h is digested；50-60 DEG C is warming up to, pH to 8 is adjusted, 0.4% alkali protease enzymolysis 1-3 h are added, go out enzyme；6000-10000 r/min centrifugations 10
- 20min, collects clear liquid, filters, and with 4 times of ethanol precipitation 1h of volume 98%, standing separation, dry sediment obtains soluble dietary fiber.
The step 2) in, in the emulsification pretreatment step, the rotating speed of emulsification pretreatment is 7000r, and the time of emulsification pretreatment is 30min.
The step 2) in, the enzyme activity of cellulase described in enzymolysis step is 30,000 U/g, and the enzyme activity of the alkali protease is 200,000 U/g.
The step 2) in, in drying steps, drying mode is at least one in forced air drying, pneumatic conveying drying, spray drying, vacuum drying and freeze-drying.
The step should control the moisture in dried product exhibited below 10%.
Beneficial effects of the present invention are as follows：
1st, this method is compared with traditional fractionation, and extraction conditions are gentle, and dietary fiber loss is few, and color and luster is good, and the waste liquid of generation is less；
2nd, this method is similar to traditional enzymatic isolation method, but the enzyme liquid of consumption is less, and product purity is higher, and soluble dietary fiber is extracted by raw material of wild pepper seed, and gained soluble dietary fiber recovery rate is more than 95%, and purity is more than 80%；
3rd, emulsification pretreatment auxiliary enzymes solution only needs to the devices such as emulsification pretreatment equipment, fermentation tank, without increasing new equipment, it is easy to industrialization.
4th, the comprehensive utilization value of wild pepper seed is improve, to wild pepper seed resource discarded object comprehensive development and utilization, low-cost, non-environmental-pollution is extracted.
Specific embodiment
With reference to specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Methods described is conventional method unless otherwise instructed.
Embodiment 1
(1) take the dry Chinese prickly ash powder of seeds of 80.00g, at 55 DEG C with petroleum ether extract twice, solid-liquid ratio 1g:
13mL, each 2.0h, filtering merge extract solution, and concentration and recovery petroleum ether obtains degreasing Chinese prickly ash powder of seeds 63.26g.
(2) 5.00g degreasing Chinese prickly ash powder of seeds is taken, pure water is added by solid-liquid ratio 1g: 20mL, rotating speed is that 7000r emulsification pretreatments are 30min；PH4.5 is adjusted with the hydrochloric acid solution of 1mol/L, 0.1g cellulases are added（Enzyme activity is 30,000 U/g）
40 DEG C, digest 10h；50 DEG C are warming up to, adjust pH to 8, add 0.02g alkali proteases（Enzyme activity is 200,000 U/g）3 h are digested, go out enzyme；6000 r/min are centrifuged 20min, collect clear liquid, filter, and with 4 times of ethanol precipitation 1h of volume 98%, standing separation is drying precipitated to obtain soluble dietary fiber 0.11g.
After testing：The soluble dietary fiber purity 81.2% for obtaining.
Embodiment 2
The degreasing Chinese prickly ash powder of seeds 10.0g of step (1) in Example 1, pure water is added by solid-liquid ratio 1g: 30mL, and rotating speed is 7000r emulsification pretreatments 30min；PH4.5 is adjusted with the hydrochloric acid solution of 1mol/L, 0.2g cellulases are added（Enzyme activity is 30,000 U/g）50 DEG C, digest 8h；60 DEG C are warming up to, adjust pH to 8, add 0.04g alkali proteases（Enzyme activity is 200,000 U/g）1 h is digested, go out enzyme；10000 r/min are centrifuged 10min, collect clear liquid, filter, and with 4 times of ethanol precipitation 1h of volume 98%, standing separation is drying precipitated to obtain soluble dietary fiber 0.21g.
After testing：The soluble dietary fiber purity 81.4% for obtaining.
Embodiment 3
The degreasing Chinese prickly ash powder of seeds 2.0g of step (1) in Example 1, pure water is added by solid-liquid ratio 1g: 25mL, and rotating speed is 7000r emulsification pretreatments 30min；PH4.5 is adjusted with the hydrochloric acid solution of 1mol/L, 0.04g cellulases are added（Enzyme activity is 30,000 U/g）45 DEG C, digest 9h；55 DEG C are warming up to, adjust pH to 8, add 0.008g alkali proteases（Enzyme activity is 200,000 U/g）1.5 h are digested, go out enzyme；8000 r/min are centrifuged 10min, collect clear liquid, filter, and with 4 times of ethanol precipitation 1h of volume 98%, standing separation is drying precipitated to obtain soluble dietary fiber 0.07g.
After testing：The soluble dietary fiber purity 81.8% for obtaining.
Claims (4)
1. a kind of preparation method of wild pepper seed soluble dietary fiber, it is characterised in that methods described comprises the following steps：
1）The preparation of degreasing wild pepper seed：The wild pepper seed crushed is weighed, with petroleum ether as extractant, solid-liquid ratio 1g：10-15ml, 55 DEG C of temperature, the h of degreasing 2；Wild pepper seed after degreasing is placed in 60 DEG C of baking ovens and is dried, be crushed to 40 mesh standby；
2）The preparation of soluble dietary fiber：Degreasing wild pepper seed powder is weighed, 20-30 times of weighing body ponding, emulsification pretreatment is added；PH 4.5 is adjusted, 2.0% cellulase is added, 40-50 DEG C, 8-10h is digested；50-60 DEG C is warming up to, pH to 8 is adjusted, 0.4% alkali protease enzymolysis 1-3 is added
H, go out enzyme；6000-10000 r/min are centrifuged 10-20min, collect clear liquid, filter, and with 4 times of ethanol precipitation 1h of volume 98%, standing separation, dry sediment obtains soluble dietary fiber.
2. a kind of preparation method of wild pepper seed soluble dietary fiber of method according to claim 1, it is characterised in that：The step 2) in, in the emulsification pretreatment step, the rotating speed of emulsification pretreatment is 7000r, and the time of emulsification pretreatment is 30min.
3. a kind of preparation method of wild pepper seed soluble dietary fiber of method according to claim 1, it is characterised in that：The step 2) in, the enzyme activity of the cellulase is 30,000 U/g, and the enzyme activity of the alkali protease is 200,000 U/g.
4. a kind of preparation method of wild pepper seed soluble dietary fiber of method according to claim 1, it is characterised in that：The step 2) in, in drying steps, drying mode is at least one in forced air drying, pneumatic conveying drying, spray drying, vacuum drying and freeze-drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878899.1A CN106820167A (en) | 2015-12-04 | 2015-12-04 | A kind of preparation method of wild pepper seed soluble dietary fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878899.1A CN106820167A (en) | 2015-12-04 | 2015-12-04 | A kind of preparation method of wild pepper seed soluble dietary fiber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106820167A true CN106820167A (en) | 2017-06-13 |
Family
ID=59149514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510878899.1A Pending CN106820167A (en) | 2015-12-04 | 2015-12-04 | A kind of preparation method of wild pepper seed soluble dietary fiber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106820167A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107183733A (en) * | 2017-06-29 | 2017-09-22 | 中国农业大学 | A kind of method for preparing capsicum seed oil and chilli seed dietary fiber |
| CN107373678A (en) * | 2017-08-21 | 2017-11-24 | 贞丰县顶罈椒业有限公司 | A kind of Chinese prickly ash diet fiber extraction method |
-
2015
- 2015-12-04 CN CN201510878899.1A patent/CN106820167A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107183733A (en) * | 2017-06-29 | 2017-09-22 | 中国农业大学 | A kind of method for preparing capsicum seed oil and chilli seed dietary fiber |
| CN107183733B (en) * | 2017-06-29 | 2021-06-18 | 中国农业大学 | Method for preparing capsicum seed oil and capsicum seed dietary fiber |
| CN107373678A (en) * | 2017-08-21 | 2017-11-24 | 贞丰县顶罈椒业有限公司 | A kind of Chinese prickly ash diet fiber extraction method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102160644B (en) | Method for preparing gold thread jujube enzyme from jujube paste of gold thread jujube | |
| CN102732585B (en) | New method for purifying fructo oligosaccharide in chicory | |
| CN105062990A (en) | Complex enzyme preparation for extracting effective plant components and method for preparing complex enzyme preparation | |
| CN102060830B (en) | Method for extracting anthocyanin from fruit dregs of Nitraria sibirica Pall | |
| CN104017098B (en) | A kind of method from mushroom waste mushroom stick extracting directly mushroom active polysaccharide | |
| CN104222353A (en) | Fuzhuan tea fragrance improving technology | |
| CN102150726A (en) | Eucommia ulmoides red tea and preparation method thereof | |
| CN104140471A (en) | Method for preparing yam high purity polysaccharide from yam food processing leftovers | |
| CN102134285A (en) | Method for preparing polysaccharide of defective porphyra haitanensis | |
| CN106832021A (en) | A kind of preparation method of Chinese cassia tree slag Thick many candies | |
| CN101698820B (en) | Method for producing white wine and fruit wine by fritillary bulb | |
| CN108142984A (en) | A kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source | |
| CN105520156A (en) | Preparation method of grape seed soluble dietary fiber | |
| CN106820167A (en) | A kind of preparation method of wild pepper seed soluble dietary fiber | |
| CN102942637A (en) | Method for increasing extraction rate of wide cactus polysaccharide | |
| CN103554287B (en) | A kind of extracting method of King Boletus mycelia polysaccharide | |
| CN103059093B (en) | Method for preparing saponin by vacuum digestion | |
| CN105132119A (en) | Method for preparing boxthorn seed oil through enzymolysis | |
| CN102643364A (en) | Method for extracting ganoderan from submerged-fermentation mycelia of ganoderma lucidum | |
| CN108640956A (en) | A method of preparing flavonoid glycoside from tea seed | |
| CN109907304A (en) | A kind of preparation method of Polysaccharide in Pleurotus eryngii | |
| CN104928331A (en) | Technology for preparing functional xylo-oligosaccharide by comprehensively utilizing wheat straw | |
| CN105039486A (en) | Method for extracting ferulic acid from wheat straw by biotechnology | |
| CN104098712A (en) | Method for efficiently extracting xylariasp mycelium polysaccharide produced through submerged fermentation | |
| CN106916233A (en) | A kind of preparation method of RADIX PUERARIAE polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170613 |
|
| WD01 | Invention patent application deemed withdrawn after publication |