CN1372595A - 在基因重组多肽的生产中抑制副产物生成的方法 - Google Patents
在基因重组多肽的生产中抑制副产物生成的方法 Download PDFInfo
- Publication number
- CN1372595A CN1372595A CN01801203A CN01801203A CN1372595A CN 1372595 A CN1372595 A CN 1372595A CN 01801203 A CN01801203 A CN 01801203A CN 01801203 A CN01801203 A CN 01801203A CN 1372595 A CN1372595 A CN 1372595A
- Authority
- CN
- China
- Prior art keywords
- polypeptide
- hanp
- serine residue
- product
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cardiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
添加氨基酸 | R1对hANP的相对比率(%) | 每单位菌体的包涵体生产量(相对比) |
不添加 | 8.97 | 1.00 |
L-赖氨酸 | 6.42 | 1.88 |
L-苏氨酸 | 5.48 | 1.95 |
L-甲硫氨酸 | 4.15 | 1.84 |
L-丙氨酸 | 5.00 | 1.30 |
L色氨酸 | 10.08 | 0.76 |
L-丝氨酸 | 5.24 | 1.24 |
L-甘氨酸 | 4.26 | 1.25 |
L-组氨酸 | 3.71 | 1.39 |
L-异亮氨酸 | 5.90 | 1.22 |
L-谷氨酸 | 5.57 | 1.68 |
L-谷氨酰胺 | 6.10 | 1.16 |
L-精氨酸 | 5.02 | 1.92 |
L-天冬氨酸 | 6.83 | 1.87 |
L-天冬酰胺 | 6.35 | 1.86 |
L-脯氨酸 | 9.01 | 1.62 |
Claims (8)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP137228/00 | 2000-05-10 | ||
JP2000137228 | 2000-05-10 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1372595A true CN1372595A (zh) | 2002-10-02 |
CN100402649C CN100402649C (zh) | 2008-07-16 |
Family
ID=18645032
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB018012035A Expired - Fee Related CN100402649C (zh) | 2000-05-10 | 2001-05-10 | 在基因重组多肽的生产中抑制副产物生成的方法 |
Country Status (12)
Country | Link |
---|---|
US (1) | US7547529B1 (zh) |
EP (1) | EP1281759B1 (zh) |
JP (1) | JP4143300B2 (zh) |
KR (1) | KR100904169B1 (zh) |
CN (1) | CN100402649C (zh) |
AT (1) | ATE435913T1 (zh) |
AU (1) | AU783954B2 (zh) |
CA (1) | CA2379100C (zh) |
DE (1) | DE60139196D1 (zh) |
ES (1) | ES2328223T3 (zh) |
IL (2) | IL147493A0 (zh) |
WO (1) | WO2001085945A1 (zh) |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ212291A (en) * | 1984-06-08 | 1991-10-25 | Suntory Ltd | Peptide from human atrium cordis having diuretic action, dna coding therefor and pharmaceutical compositions thereof |
JPS61119189A (ja) * | 1984-11-15 | 1986-06-06 | Green Cross Corp:The | プラスミノ−ゲンアクチベ−タ−前駆体の産生増強方法 |
EP0440311A1 (en) * | 1985-06-20 | 1991-08-07 | Fujisawa Pharmaceutical Co., Ltd. | A process for the production of alpha-human atrial natriuretic polypeptide |
GB8518753D0 (en) * | 1985-07-24 | 1985-08-29 | Glaxo Group Ltd | Microbiological products |
JPS6244198A (ja) * | 1985-08-23 | 1987-02-26 | Toray Ind Inc | 有用タンパク質の生産方法 |
DK175535B1 (da) | 1987-03-04 | 2004-11-29 | Daiichi Suntory Pharma Co Ltd | Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid |
US5599690A (en) | 1988-02-01 | 1997-02-04 | Amgen Inc. | Control of norleucine incorporation into recombinant proteins |
US5622845A (en) | 1988-02-17 | 1997-04-22 | The Upjohn Company | Fermentation method for producing norleucine |
ATE152176T1 (de) | 1988-02-17 | 1997-05-15 | Upjohn Co | Verfahren zum regeln des norleucingehalts in polypeptiden |
US5169772A (en) * | 1988-06-06 | 1992-12-08 | Massachusetts Institute Of Technology | Large scale method for purification of high purity heparinase from flavobacterium heparinum |
ES2091241T3 (es) * | 1989-03-15 | 1996-11-01 | Upjohn Co | Polipeptidos heterologos acetilados. |
JP3531947B2 (ja) * | 1991-08-19 | 2004-05-31 | 第一サントリーファーマ株式会社 | ペプチドの製造方法 |
TW224042B (zh) | 1992-04-04 | 1994-05-21 | Basf Ag | |
JPH07308199A (ja) * | 1994-05-18 | 1995-11-28 | Green Cross Corp:The | ヒト血清アルブミンの製造方法 |
-
2001
- 2001-05-10 US US10/030,452 patent/US7547529B1/en not_active Expired - Fee Related
- 2001-05-10 JP JP2001582534A patent/JP4143300B2/ja not_active Expired - Lifetime
- 2001-05-10 KR KR1020027000330A patent/KR100904169B1/ko not_active IP Right Cessation
- 2001-05-10 ES ES01930035T patent/ES2328223T3/es not_active Expired - Lifetime
- 2001-05-10 IL IL14749301A patent/IL147493A0/xx active IP Right Grant
- 2001-05-10 DE DE60139196T patent/DE60139196D1/de not_active Expired - Lifetime
- 2001-05-10 CN CNB018012035A patent/CN100402649C/zh not_active Expired - Fee Related
- 2001-05-10 AT AT01930035T patent/ATE435913T1/de active
- 2001-05-10 AU AU56692/01A patent/AU783954B2/en not_active Ceased
- 2001-05-10 CA CA2379100A patent/CA2379100C/en not_active Expired - Fee Related
- 2001-05-10 WO PCT/JP2001/003909 patent/WO2001085945A1/ja active IP Right Grant
- 2001-05-10 EP EP01930035A patent/EP1281759B1/en not_active Revoked
-
2002
- 2002-01-06 IL IL147493A patent/IL147493A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AU783954B2 (en) | 2006-01-05 |
AU5669201A (en) | 2001-11-20 |
EP1281759B1 (en) | 2009-07-08 |
WO2001085945A1 (fr) | 2001-11-15 |
JP4143300B2 (ja) | 2008-09-03 |
IL147493A0 (en) | 2002-08-14 |
EP1281759A4 (en) | 2004-09-08 |
CN100402649C (zh) | 2008-07-16 |
CA2379100A1 (en) | 2001-11-15 |
CA2379100C (en) | 2011-11-01 |
US7547529B1 (en) | 2009-06-16 |
ATE435913T1 (de) | 2009-07-15 |
IL147493A (en) | 2011-02-28 |
EP1281759A1 (en) | 2003-02-05 |
KR20020034154A (ko) | 2002-05-08 |
KR100904169B1 (ko) | 2009-06-22 |
DE60139196D1 (de) | 2009-08-20 |
ES2328223T3 (es) | 2009-11-11 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
ASS | Succession or assignment of patent right |
Owner name: SUNTORY LTD Free format text: FORMER OWNER: SUNTORY LTD. Effective date: 20030526 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20030526 Applicant after: Daiichi Suntory Pharma Co., Ltd. Applicant before: Suntory Ltd. |
|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: SANKYO CO. Free format text: FORMER OWNER: ASUBIO PHARMA CO., LTD. Effective date: 20100916 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20100916 Address after: Tokyo, Japan Patentee after: Sankyo Co. Address before: Tokyo, Japan Patentee before: Asubio Pharma Co., Ltd. |
|
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080716 Termination date: 20170510 |
|
CF01 | Termination of patent right due to non-payment of annual fee |