CN1358729A - Process for extracting raphanin heteroxide from zhugecai vegetable - Google Patents
Process for extracting raphanin heteroxide from zhugecai vegetable Download PDFInfo
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- CN1358729A CN1358729A CN 01129168 CN01129168A CN1358729A CN 1358729 A CN1358729 A CN 1358729A CN 01129168 CN01129168 CN 01129168 CN 01129168 A CN01129168 A CN 01129168A CN 1358729 A CN1358729 A CN 1358729A
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- glucoraphanin
- extracting
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- zhugecai
- slurries
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The method for extracting radish seed glucoside from zhugecai (a Chinese plant) includes the following steps: soaking zhugecai seed in hot water for 3-5 min., using homogenizer to homogenize for 10 min., placing the obtained slurry liquor into a container, adding hot water into residue and making homogenization, pouring said slurry into the above-mentioned container, repeating the above-mentioned operation once, centrifugal treatment, filtering supernatant fluid, low-temp. drying filtrate so as to obtain powder containing radish seed glucoside.
Description
The present invention relates to a kind of method of from plant, extracting glucoraphanin.
Glucoraphanin (glucoraphanin) has good anticancer function, its main effect one is the dna damage that reduces target tissue by regulatory enzyme, stop cell internalizing to learn the generation of carcinogenic substance, the 2nd, the development of cancer cells before suppressing, apoptosis by cancer cells before inducing, cut off its asexual amplification, thereby eliminate the preceding cancer cells of genetic damage tissue.United States Patent (USP) 6242018,6177122,5968567,5882646,5968505,5725895, disclosing for No. 568618 with the plant wild cabbage with glucoraphanin is that raw material extracts the method that glucoraphanin is processed anti-cancer health-care food, though and the wild cabbage range of distribution is wider, wherein contain the kind narrow distribution of glucoraphanin, and growth is relatively poor in barren soil, particularly in the vast karst region of China.In addition, United States Patent (USP) is raw materials used to be plant plumule, and its production difficulty is big, the preservation difficulty.
The objective of the invention is: it is the method that raw material extracts the cancer-resisting substance glucoraphanin that a kind of plant Orychophragmus violaceus (Orychophragmus violaceus) that all is distributed with China 20 several provinces is provided, and its content height, and cultivation is convenient to preserve easily.
The present invention is achieved in that getting orychophiagmus violaceus seed put into hot-water soak 3～5 minutes, with homogenizer homogenate 10 minutes, slurries are poured in the container, residue adds hot water homogenate again, slurries are poured container again into, and said process repeats to do once again, the capable again centrifugal treating of the slurries in the container, the gained supernatant liquor filters, and filtrate obtains to contain the powder of glucoraphanin again with cryodrying.
The also available conventional supercritical CO of said process
2Extraction process is realized; Used raw material is parent's a hybrid plant with the Orychophragmus violaceus also.
Advantages such as the present invention compared with prior art has raw material and is easy to get, and is convenient to preserve, and production method is simple.Because Orychophragmus violaceus has the suitable natural disposition of karst, it can be grown on the barren limestone soil of soil, and can optimize edatope.The used raw material of the present invention is the seed of plant, and is obviously easy than the young shoot production with plant.In addition, Orychophragmus violaceus also has diversified economy to be worth, and can do to cover plant, health-care vegetable etc. are used.
Embodiments of the invention: get 2～4 kilograms of orychophiagmus violaceus seeds, putting into 100 ℃ of hot water soaked 3～5 minutes for 5 liters, with homogenizer homogenate 10 minutes, slurries are poured in the container, residue adds 5 liters of homogenate of 100 ℃ of hot water 10 minutes again, slurries are poured container again into, said process repeats to do once again, slurries in the container were used the whizzer centrifugal treating 5 minutes again, the gained supernatant liquor filters with filter paper, the filtrate that must contain glucoraphanin, filtrate obtain to contain the powder of glucoraphanin again with cryodrying.
The also available conventional supercritical CO of said process
2Extraction process is realized; Used raw material is parent's a hybrid plant with the Orychophragmus violaceus also, as rape and Orychophragmus violaceus remote hybrid etc.
Claims (3)
1, a kind of method of from Orychophragmus violaceus, extracting glucoraphanin, it is characterized in that: get orychophiagmus violaceus seed and put into hot-water soak 3～5 minutes, with homogenizer homogenate 10 minutes, slurries are poured in the container, and residue adds hot water homogenate again, and slurries are poured container again into, said process repeats to do once again, the capable again centrifugal treating of slurries in the container, the gained supernatant liquor filters, and filtrate obtains to contain the powder of glucoraphanin again with cryodrying.
2, the method for extracting glucoraphanin from Orychophragmus violaceus according to claim 1, it is characterized in that: said process is the supercritical CO of available routine also
2Extraction process is realized.
3, the method for extracting glucoraphanin from Orychophragmus violaceus according to claim 1 and 2 is characterized in that: used raw material is parent's a hybrid plant with the Orychophragmus violaceus also.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011291680A CN1161365C (en) | 2001-12-07 | 2001-12-07 | Process for extracting raphanin heteroxide from zhugecai vegetable |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011291680A CN1161365C (en) | 2001-12-07 | 2001-12-07 | Process for extracting raphanin heteroxide from zhugecai vegetable |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1358729A true CN1358729A (en) | 2002-07-17 |
| CN1161365C CN1161365C (en) | 2004-08-11 |
Family
ID=4668960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011291680A Expired - Fee Related CN1161365C (en) | 2001-12-07 | 2001-12-07 | Process for extracting raphanin heteroxide from zhugecai vegetable |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1161365C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014036808A1 (en) * | 2012-09-07 | 2014-03-13 | 北京健旭康技术有限公司 | Plant beneficial to liver and use thereof |
| CN104490914A (en) * | 2014-11-11 | 2015-04-08 | 济南星懿医药技术有限公司 | Anti-tumor pharmaceutical composition |
| CN104523720A (en) * | 2014-11-11 | 2015-04-22 | 济南星懿医药技术有限公司 | Anticancer pharmaceutical composition |
-
2001
- 2001-12-07 CN CNB011291680A patent/CN1161365C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014036808A1 (en) * | 2012-09-07 | 2014-03-13 | 北京健旭康技术有限公司 | Plant beneficial to liver and use thereof |
| CN104490914A (en) * | 2014-11-11 | 2015-04-08 | 济南星懿医药技术有限公司 | Anti-tumor pharmaceutical composition |
| CN104523720A (en) * | 2014-11-11 | 2015-04-22 | 济南星懿医药技术有限公司 | Anticancer pharmaceutical composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1161365C (en) | 2004-08-11 |
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| C06 | Publication | ||
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |