WO2014036808A1

WO2014036808A1 - Plant beneficial to liver and use thereof

Info

Publication number: WO2014036808A1
Application number: PCT/CN2013/001041
Authority: WO
Inventors: 张莘; 张义飞
Original assignee: 北京健旭康技术有限公司
Priority date: 2012-09-07
Filing date: 2013-09-05
Publication date: 2014-03-13
Also published as: CN103655666B; CN103655666A

Abstract

A use of Oiychophragmus violaceus for preparing a plant productbeneficial to livers of the human and animals. Various plant products beneficial to the livers are prepared with the whole herb or a part (root, stem and leaf, sprout and seedling, flower and seed) of Oiychophragmus violaceus as a raw material, and through washing, drying, or further water decoction and extraction. Dosage forms of the products comprise health food, Chinese herbal pieces and injections, granule, tablets, capsules, pills, powder and the like. The products can treat liver cirrhosis, virus B hepatitis, alcoholic hepatitis and toxic hepatitis caused by carbon tetrachloride.

Description

Plant with beneficial liver function and application thereof

The invention relates to a processing technology of a wild plant "Yigancao" with beneficial liver function and the use in food, health food, natural medicine, veterinary health care product and veterinary medicine, in particular, fresh and dry liver Vegetable whole grass, deep processing and extract preparation method, Yigan vegetable deep processing product, Yigan vegetable extract, effective chemical composition of Yigancai, use of Yigancao in preparation of liver-protecting health care products, and preparation of hepatitis in Yigancai Applications of therapeutic drugs ('. and a class of natural drugs) and applications of animal liver products (eg, prevention of duckling viral hepatitis and toxic hepatitis). Background technique

The liver is one of the important organs in the body. It is subjected to a large amount of biochemical chemistry every day. It is a T. work of the factory. It is very easy to fatigue and suffer from the processing of a large amount of foods and other foreign substances absorbed by the digestive tract. damage. Liver damage is a disease that seriously harms people's health. China currently has more than 100 million people living with hepatitis B virus and tens of millions of hepatitis C virus carriers. About tens of millions of them will be converted into chronic hepatitis, cirrhosis, liver cancer, and people who die every year due to liver disease. There are hundreds of thousands of people. In recent years, toxic liver damage caused by alcohol, drugs and various harmful factors has also increased. The cost of treating liver disease in China every year is as high as tens of billions of yuan, which puts a heavy burden on economic and social development, especially the physical and mental health of patients. Therefore, maintaining the health of the liver has become the goal pursued by people. Food and medicine are important factors affecting liver function, so it is urgent to develop foods, health foods and new medicines that are good for liver health.

Yiyin (Oryc hmgm νίο! D OE Sc lz : Yigancai is a cruciferous herb. Its Chinese name is - . Moon Blue (Beijing Flora) and ". Yuelan (Liao Shi' Flora The origin of Yigancai is in Northeast China and North China. There are no research reports on the consumption or medicinal Yigancai in many areas such as Beijing where Yigancai is grown. According to the Agricultural Plant Variety Nomenclature issued by the Ministry of Agriculture. 3⁄4》 (Organization of the Ministry of Agriculture of the People's Republic of China, 2012, No. 2, since April 15, 2012), we believe that Yigancai is a new resource for edible and medicinal new varieties of agricultural plants. "Liver" is the name we found after the plant has a good liver function.

In addition, Yigancao has no application in health foods and medicinal purposes. The present invention finds new uses, processing methods, and active ingredients contained in edible foods, foods, and foods. Summary of the invention

The present invention provides a new use of Yigancai, namely, new J and V in the preparation of hepatitis therapeutic drugs, health foods and animal liver products.

Another object of the present invention is to provide a medicament for treating hepatitis. Still another aspect of the present invention is to provide a health food for hepatitis adjuvant therapy.

The object of the present invention is also to provide a beneficial liver preparation, a health care and a therapeutic effect, and a preparation method thereof. The inventor conducted a large number of deep processing studies on the whole process and various parts of Yigancai according to the processing method of agricultural products, including Yigancao porridge, Yigancao soup, Yigancai juice (fresh juicing), dried Yigancai, Instant Yigancao, Yigancai wine, Yigancai pickled products, Yigancai decoction pieces, etc. The corresponding products can be harvested at various stages of the growth of the beneficial liver.

Yigan can be eaten directly or directly in its dry plants.

The inventor refers to the 2003 Guidelines for the Technical Guidance of Drug Research, the Chinese Medicine and Natural Substance (National Food and Drug Administration, 2003), the Guiding Principles for Preclinical Research of New Drugs (National Food and Drug Administration, 1993) and Health Food Function. The evaluation method of the evaluation (Ministry of Health 2003), the drug evaluation method for hepatitis treatment, and the health food evaluation method, the extract preparation, the pharmaceutical dosage form preparation, the animal pharmacology and the pathological study of the beneficial liver.

The inventors found that various parts of the beneficial liver are beneficial to the liver, which are the flowers, seeds, leaves, stems, roots and sprouts of the plants. The whole grass or part of Yigancai can be made into pieces of natural medicine. Harvesting of beneficial liver vegetables can be carried out all year round. Harvesting the characteristics of the pieces produced in Ding: Cut the same grass into small pieces, net, and dry. Yigancao seed pieces are net and dry. The Yigancao Pieces can be used directly or directly after eating the dried pieces.

The preparation method of Yigancao extract is as follows: You can take 1000g of Yigancao decoction pieces or appropriate amount of fresh whole grass, crush, wash 3~4 times, add water for three times, each time for 15 minutes, combine decoction, filter After that, the filtrate is concentrated and thickened, and after drying, a fine powder is obtained. This product is brown granules; sweet and sweet.

The inventors found that oral administration and injection administration of Yigancao extract have obvious effects, and serum liver biochemical function indexes and pathological indexes are significantly improved.

When it is treated with Yigancao extract, it can be administered orally in the form of powder, granules, tablets, capsules, pills and liquid medicaments; it can also be in the form of injections, suppositories, transdermal preparations, sprays, etc. Administered enterally. An effective agent for the extract of yoghurt is prepared by mixing with a suitable pharmaceutical blend such as an excipient, a binder, a penetrant, a lubricant or the like.

The dose of the beneficial liver extract varies depending on the condition of the patient, the route of administration, the age of the patient, and the body S. In the case of oral administration, the dose is usually from 500 to 1000 mg per day for adults, preferably 800 mg per day. The same principle applies to health food use.

Advantages of the invention: The beneficial liver treatment effect of Yigancai is first discovered both domestically and internationally. Yigancao can be used as a natural medicine for the treatment of hepatitis B, for the treatment of cirrhosis, for the treatment of toxic hepatitis such as alcoholic hepatitis and carbon tetrachloride. There are currently no internationally recognized natural medicines for hepatitis B and cirrhosis and 3⁄4 hepatitis in alcohol.

In addition, the preparation method of Yigancao is relatively simple. The research on plant growth characteristics is also relatively thorough. With a certain scale of planting base, the source and economy of the materials are guaranteed. The market identity of natural products is relatively high, the market acceptance is high, and the market prospects are good. detailed description

The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. Example 1. Material

The Yigancao pieces used in the examples were made by ourselves. Different parts such as whole liver grass or seeds are used. We have established the seasons for harvesting different parts of the beneficial liver and the operating standards for the production of the medicinal herbs in different parts. Simply put, the main game in autumn is to harvest the stems and leaves. Spring harvesting Whole grass including roots and pods. Harvest seeds in summer. After washing the stems and roots, cut into small pieces of 2 cm and dry. The seed pieces are made through a dry and net selection process.

Other drugs, reagents, and laboratory animals are readily available commercially.

3⁄4 Example 2. Example of preparation method

Take 1000g of lettuce and liver grass, smash, wash 3 times, add 2500ml of water to cook, three times each time, 20 minutes each time, combine the decoction, filter, concentrate the filtrate into a thick paste with a relative density of 1.18, continue to decompress Concentrated to dryness to give a brown powder of 51 g. Example 3. Formulation Example,

(1) taking the preparation method of the lkg Example 2 to obtain the extract of the beneficial liver of each plant part, pulverizing and passing through a 100 mesh sieve, and mixing into a proper amount of starch to prepare each granule, and then granulating the whole granules, 2 g per bag .

(2) 1000 g of the extract of Yigancai obtained by the preparation method of Example 2, 10 g of hydroxyethylcellulose, and tableted into tablets.

(3) The extract of the beneficial liver obtained by the preparation method of Example 2 is dissolved in physiological saline or isotonic glucose solution, and after filtration, the prepared injection solution is used as needed.

(4) The extract of the beneficial liver obtained by the preparation method of the second embodiment is dissolved in water, and can be directly formulated into a U solution.

(5) The extract of the beneficial liver obtained by the preparation method of the second embodiment can be combined with a drug such as licorice extract and astragalus extract to prepare a compound for treating hepatitis. Example 4. Pharmacodynamic experiment

(1) Evaluation method

Study on the efficacy of Yigancai and its extracts in the treatment of hepatitis

The experimental animals were adult healthy male CD-I mice. During the test period, I'Hj provided a sufficient amount of tap water and ordinary feed. Grouping and Processing of Animals Mice were randomized by body weight. In the observation of the efficacy of toxic hepatitis, it was administered for 3 consecutive days, once a day. On the fourth day, blood was taken and the liver of the animal was collected. The liver function enzyme alanine transferase (ALT) was measured in the serum, and the degree of liver pathological changes was observed. The administration time of the evaluation of '. methyl nitrosamine cirrhosis treatment was 2 months, and then the degree of fibrosis in the liver tissue (hydroxyproline) was measured. Hepatitis B efficacy evaluation using fine Cell evaluation method. Experimental data is expressed as mean ± standard deviation. The results of the experiment were analyzed by ANOVA and t-test using the EXCEL statistical software. The difference of the group I'Hj was considered to be significant at p<0.05.

(2) Evaluation results: Table 1 Effect of extracts of Yigancao grass and extracts from various parts on carbon tetrachloride poisoning hepatitis Extract classification ALT

(plant g/kg) (1U L)

Control group 35 people 20

Carbon tetrachloride hepatitis group 2680±778 ^# *

Same grass extract low dose 2375±1089

Whole herb extract 3⁄4 661±659**

Whole grass extract high dose 102±63**

Root extract dose 532±269**

Stem and leaf extract dose 898±5 S5i8**

Flower extract agent fi 1665±1126* oo

The dose in the seed extract was 181±88**. Note: ^#Compared with the control group, p < 0.05. ' |=* is compared with the model group p < 0.01.

Table 2 Yigancao whole grass seeds anti-alcoholic mice toxic hepatitis effect Yigancao seed extract ALT

(plant g/kg) (IU/L)

Control 绗 50±15

Alcohol hepatitis group 101±60

Low dose 47±12*

Medium dose 50±20*

High dose *

Note: ^#Compared with the control group, p < 0.05. *Compared with the model group p < 0.05. Effect of Yigancao Seed Extract on Dimethylnitrosamine Liver Cirrhosis Yigancao Seed Extract Hydroxyproline

(plant g/kg) (mg g) control group 0.10±0.04 cirrhosis group 0.23±0.07 ^# * low dose 0.20±0.56 medium dose 0.18±0.05* high Qi! j halo 0.15±0.02** Note: ^##与对照Group comparison p < 0.01. * , ** compared with the model group p < 0.05, p < 0.01. Table 4 Cytopathic Yigancao Seed Extract of Yigancao Seed Extract against Virus Inhibition of Hepatitis B

Concentration (mmol) HBsAg

Empty control 2.284±0.194

Low concentration 1.407±0.578* medium concentration 1.331±0.393** medium concentration 1.106±0.245** high concentration 0.678±0.026** compared with empty Ci control (cell), *p<0.05, **p<0.01

Claims

Claim

1. Use Ding to prepare a plant product having a beneficial effect on humans and animals, characterized by an extract containing whole grass or parts of Yigancai.

2. A Yigancai decoction piece, characterized in that all the grass or parts of Yigancai can be made into natural pieces. The characteristics of the preparation of the pieces are as follows: the cut grass is small, net, and dry. Yigancao seed pieces are net and dry. The parts of the beneficial liver are roots, stems and leaves (including seedlings), flowers, seed pods and seeds. Yigancao decoction pieces can be used directly or directly after eating the dried pieces.

3. An extract of Yigancai, characterized in that it is prepared by the following method, taking the pieces of fresh liver or the whole whole grass, net, crushing, washing 3~4 times, boiling water three times, each time After 15 minutes, the decoction was combined, filtered, and the filtrate was concentrated. Or dry the thick paste to make a fine powder.

4. The beneficial liver extract according to claim 3, which is characterized in that it can be formulated into an oral preparation, an injection, a granule, a tablet, a capsule, a pill, together with an extract fine powder and a suitable medicinal dressing. A variety of dosage forms such as powder. It can also be combined with other drugs or other plant extracts to form a compound preparation.

A medicament for adjuvant therapy of hepatitis, which comprises the beneficial liver flakes or extract of claims 2 and 3.

A health product for benefiting liver, which comprises the beneficial liver flakes or extract of claims 2 and 3.

7. The use of Yigancao decoction pieces or extracts described in Yigancai or the rights of 2 and 3 in the manufacture of therapeutic drugs for various hepatitis B and health care products.

8. The use of Yigancao or the extracts of Yigancao or the extracts according to claims 2 and 3 for the preparation of a therapeutic drug for toxic hepatitis and a health care product, preferably the toxic hepatitis of the towel, alcoholic toxic hepatitis, Carbon tetrachloride toxic hepatitis.

9. Use of Yigancao or the beneficial liver tablets or extracts according to claims 3 and 3 for the preparation of cirrhosis therapeutic drugs and health care products.