CN1358426A - Quick culture method for edible cactus - Google Patents
Quick culture method for edible cactus Download PDFInfo
- Publication number
- CN1358426A CN1358426A CN 00131270 CN00131270A CN1358426A CN 1358426 A CN1358426 A CN 1358426A CN 00131270 CN00131270 CN 00131270 CN 00131270 A CN00131270 A CN 00131270A CN 1358426 A CN1358426 A CN 1358426A
- Authority
- CN
- China
- Prior art keywords
- cardoncillo
- cut
- edible
- cactus
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a fast reproductive method for edible cactus. Taking the edible part of cactus as the explant and using self medium which includes MS, as the basic meidum and plus incoproated material with different ingredients to speed up the reproduction of edible cactus. Its specific steps is as follows: to cut the edible cactus into small pieces, to sterilize with 0.1 % mercuric chloride, to be washed with germ-free water; and then cut the small pieces into pieces again with female dochium and to be connected with medium and be cultivated by light. With this method a new bud can produce 10 million seedlings each year.
Description
The present invention relates to the plant seedling breeding, more specifically relate to a kind of method for quickly breeding of cardoncillo.
Cardoncillo belongs to cactaceous succulent, is in recent years from the new varieties of external introduction, and work done in the manner of a certain author is that a kind of special vegetable is promoted in China, and has edible and medicinal function concurrently.Cardoncillo generally adopts the method for cutting cuttage to breed, and an Indian fig can only breed the 3-4 piece in 1 year.Because reproduction coefficient is low, can not satisfy the demand of market to seedling.Tissue culture belongs to biological technical field, it is a totipotency of utilizing plant cell, the little block organization of cultured in vitro plant carries out the fast numerous industrial breeding technique of plant clone, and it is the most ripe, a reliable biotechnology that applies to agricultural production up to now.Be mainly used in top grade, good plant at present as the fast breeding of the plant seedling that added values such as fruit tree, flowers, forest and rare traditional Chinese medicine are high.The method for quickly breeding of not finding a kind of cardoncillo by retrieval is disclosed.
The purpose of this invention is to provide a kind of method for quickly breeding of cardoncillo, easy to implement the method, easy to operate, reproduction speed is fast, for the cardoncillo factorial seedling growth has been opened up approach.
The invention is characterized in, cardoncillo is cut into small pieces, sterilize with 0.1% mercury chloride, use aseptic water washing, the fritter that is cut into is cut into fritter with female seat again, insert in the prepared culture medium, this medium comprises the material of MS minimal medium and additional different components, illumination cultivation.
In the procedure that the present invention proposes, as explant, at first cardoncillo is cut into the fritter of 3-4cm with Indian fig edible part, with 0.1% mercury chloride sterilization 8-15 minute, use aseptic water washing 4-5 time; Next is that the fritter that will be cut into is cut into the fritter of 1-2cm with female seat again, inserts in the prepared culture medium, and this medium comprises the material of MS minimal medium and additional different components; The 3rd is illumination cultivation, and temperature is 20-26 ℃, light intensity 1000-2000Lx, and illumination every day 10-12 hour is cultivated and can be grown sprouting in 7-14 days.
The present invention compared with prior art, have the following advantages and effect, method is easy, and is easy to operate, and reproduction speed is fast, can breed once in 30 days, the one-step growth coefficient reaches 8-10 doubly, can breed for 12 generations in 1 year, and a sprouting can breed 1,000 ten thousand seedlings in 1 year, improved the production capacity of seedling greatly, be beneficial to factory's commercialization and produce.
Embodiment
As explant, at first cardoncillo is cut into the fritter of 3-4cm with plant Indian fig edible part,, uses aseptic water washing 4-5 time with 0.1% mercury chloride sterilization 8-15 minute; Next is that the fritter that will be cut into is cut into the fritter of 1-2cm with female seat again, inserts in the prepared culture medium, and this medium comprises the material of MS minimal medium and additional different components.
The composition of MS minimal medium (unit: mg/L):
NH
4NO
3 1650
KNO
3 1900
CaCl
2·2H
2O 440
MgSO
4·7H
2O 370
KH
2PO
4 170
KI 0.83
H
3BO
3 6.2
MnSO
4·4H
2O 22.3
ZnSO
4·7H
2O 8.6
Na
2MoO
4·2H
2O 0.25
CuSO
4·5H
2O 0.025
CoCl
2·6H
2O 0.025
FeSO
4·7H
2O 27.8
Na
2·EDTA·2H
2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2 additional compositions (unit: mg/L):
BA 1-4
NAA 0.01-0.4
Agar 6000-8000
Sugar 20000-40000 or
IBA 0.1-0.5
NAA 0.01-0.5 the 3rd is an illumination cultivation, and temperature is 20-26 ℃, and light intensity is 1000-2000Lx, and illumination every day 10-12 hour is cultivated and can be grown sprouting in 7-14 days, expands once numerous in then every 30-40 days.
Claims (4)
1, a kind of method for quickly breeding of cardoncillo is characterized in that cardoncillo is cut into small pieces, and with 0.1% mercury chloride sterilization, use aseptic water washing, and the fritter that is cut into is cut into fritter with female seat again, in the access prepared culture medium, and illumination cultivation.
2, the method for quickly breeding of a kind of cardoncillo according to claim 1 is characterized in that the effective ingredient in the medium:
BA 1-4mg/L
NAA 0.01-0.4mg/L
Agar 6000-8000mg/L
Sugar 20000-40000mg/L
3, the method for quickly breeding of a kind of cardoncillo according to claim 1 is characterized in that the effective ingredient in the medium:
IBA 0.1-0/5mg/L
NAA 0.01-0.5mg/L
4, the method for quickly breeding of a kind of cardoncillo according to claim 1 is characterized in that illumination cultivation, and temperature is 20-26 ℃, light intensity 1000-2000Lx, and illumination every day 10-12 hour was cultivated 7-14 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00131270 CN1358426A (en) | 2000-12-15 | 2000-12-15 | Quick culture method for edible cactus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00131270 CN1358426A (en) | 2000-12-15 | 2000-12-15 | Quick culture method for edible cactus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1358426A true CN1358426A (en) | 2002-07-17 |
Family
ID=4594591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00131270 Pending CN1358426A (en) | 2000-12-15 | 2000-12-15 | Quick culture method for edible cactus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1358426A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627727B (en) * | 2009-08-25 | 2012-06-20 | 山东省烟台农业学校 | Method for preventing and controlling tissue culture seedling pollution |
| CN103548651A (en) * | 2013-10-23 | 2014-02-05 | 镇江市丹徒区紫杉生态农业园 | Nutrient solution cultivation method for cactuse |
-
2000
- 2000-12-15 CN CN 00131270 patent/CN1358426A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627727B (en) * | 2009-08-25 | 2012-06-20 | 山东省烟台农业学校 | Method for preventing and controlling tissue culture seedling pollution |
| CN103548651A (en) * | 2013-10-23 | 2014-02-05 | 镇江市丹徒区紫杉生态农业园 | Nutrient solution cultivation method for cactuse |
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|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |