CN1133362C - Quick reproducing method for magnolia flower - Google Patents
Quick reproducing method for magnolia flower Download PDFInfo
- Publication number
- CN1133362C CN1133362C CNB001312693A CN00131269A CN1133362C CN 1133362 C CN1133362 C CN 1133362C CN B001312693 A CNB001312693 A CN B001312693A CN 00131269 A CN00131269 A CN 00131269A CN 1133362 C CN1133362 C CN 1133362C
- Authority
- CN
- China
- Prior art keywords
- sprout
- cut
- flower bud
- lily magnolia
- young sprout
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses a quick propagation method for a magnolia, which uses a new young sprout of a plant as an external brood body. The present invention comprises the following steps: firstly, the young sprout of the magnolia is cut into sprout segments, and then, the sprout segments are sterilized by 0.1% of mercury chloride and are flushed by sterilized water; secondly, the new young sprout segments are cut into sprout sections, and then, the sprout sections are added into a culture medium; thirdly, the sprout sections are illuminated by light and are cultivated, the sprout sections are illuminated by light every day, and are cultivated for 7 to 14 days; fourthly, tender sprouts are inoculated into the culture medium. The method of the present invention has the advantages of easy realization, convenient operation, high propagation speed and high survival rate.
Description
Technical field
The invention belongs to the plant seedling breeding, do not relate to plant variety, the flower bud of lily magnolia is bred fast by minimal medium and additional different components.
Background technology
The flower bud of lily magnolia belongs to Magnoliacea plant, is a kind of outstanding Landscape Tree Species and Chinese herbal medicine commonly used, also is one of the appointment plantation medicinal material kind in Hubei Province.Tissue culture belongs to biological technical field, it is a totipotency of utilizing plant cell, by the little block organization of cultured in vitro plant, carry out the fast numerous industrial breeding technique of plant clone, it is the most ripe, a reliable biotechnology that applies to agricultural production up to now.Be mainly used in top grade, good plant at present as the fast breeding of the plant seedling that added values such as fruit tree, flowers, forest and rare traditional Chinese medicine are high.
Two kinds of now many employing low-pressure processes of the flower bud of lily magnolia and high-pressure processes.Low-pressure process is: cultivate elite stand earlier and be the shrub shape, early spring, the zanjon of 8-10cm was dug near ground elite stand, branch bending is arrived bottom of trench, and be fixed in the soil with wooden hook, the Xiang Duan of branch is basseted cover fertile soil then, having peace of mind is watered, and frequent tending management can be taken root and maternal plant is separated into seedling through 1-2.High-pressure process is: on the not flexible elite stand that goes into ground of branch, select annual branch in vegetative period, carry out ringing (wide 1-1.5cm), liver moss wraps in external application, wrap film then or tie up on branch with thick bamboo tube and the special half sheet flowerpot of making, fill out again and grow soil or mountain mud, wait to take root the back cut off in the lower end, a strain new talent.Above-mentioned two kinds of methods, operation is loaded down with trivial details, the time is long, 2-3 could Cheng Miao, and a strain elite stand to can be used for the material of press strip in 1 year limited.Another kind is a graft seedling growth: make stock with lily magnolia usually.Stock is educated in sowing in 1 year, grows and carries out grafting after 1 year again, generally to carry out carrying out cut-grafting in bud grafting or 1 year spring autumn, needs 2 years ability Cheng Miao; Another is that the cottage propagation consumptive material is many and survival rate is lower.So the flower bud of lily magnolia is relatively more difficult with the conventional method breeding, required time is long, and needs a large amount of maternal plants, has so not only destroyed the tree body, has caused the waste of plant resources again.
Summary of the invention
The purpose of this invention is to provide a kind of propagation method of the flower bud of lily magnolia, easy to implement the method, easy to operate, the breeding time is short, the survival rate height.
In order to achieve the above object, the present invention adopts following technical measures: with flower bud of lily magnolia young sprout as explant, young sprout is cut into the long stem section of 2-3cm, with 0.1% mercury chloride sterilization 5-10 minute, with aseptic water washing 3-4 time, again the young sprout section is cut into 1-2 bud one joint, insert in the prepared culture medium, this medium comprises the material of MS minimal medium and additional different components, illumination cultivation, temperature is 20-28 ℃, light intensity 1500-3000Lx, illumination every day 10-16 hour was cultivated 7-14 days, its lateral bud grows young sprout, expands once numerous in later every 30-40 days.Get the tender tip, be inoculated in and cultivate rooting and transplant after 10-20 days in the root media but get the long tender tip of 1-5cm.
The present invention compared with prior art has the following advantages and effect, and easy to implement the method, easy to operate, reproduction speed is fast, and 30 days propagation multiple reaches 4-5 doubly, and a bud can be bred 1,000,000 strain seedlings, survival rate height in 1 year.
Embodiment
Embodiment:
With the flower bud of lily magnolia young sprout of plant as explant, at first young sprout is cut into the long stem section of 2-3cm, with 0.1% mercury chloride sterilization 5-10 minute, with aseptic water washing 3-4 time, next is that the young sprout section is cut into 1-2 bud one joint, inserts in the prepared culture medium, and this medium comprises the material of MS minimal medium and additional different components, wherein additional composition is BA0.2-4mg/L, NAA0.01-0.4mg/L, agar 6000-8000mg/L, and sugared 20000-30000mg/L is mixed.Adopting another additional composition is that IBA0.1-0.5mg/L, Hua Lin-431-5ml/L, agar 6000-8000mg/L, sugared 20000-30000mg/L are mixed; The 3rd is illumination cultivation, and temperature is 20-28 ℃, and light intensity is 1500-3000Lx, cultivates 7-14 days under 10-16 hour the condition of illumination every day, makes lateral bud grow young sprout, expands once numerous in later every 30-40 days; The 4th is to get the tender tip, getting the long tender tip of 1-5cm is inoculated in the minimal medium of taking root, this medium comprises the material of MS minimal medium and additional composition, additional composition is IBA0.1-0.5mg/L, Hua Lin-43 1-5ml/L, agar 6000-8000mg/L, sugared 20000-30000mg/L, after 10-20 days, but rooting and transplant.
The composition of MS minimal medium (unit: mg/L):
NH
4NO
3 1650
KNO
3 1900
CaCl
2·2H
2O 440
MgSO
4·7H
2O 370
KH
2PO
4 170
KI 0.83
H
3BO
3 6.2
MnSO
4·4H
2O 22.3
ZnSO
4·7H
2O 8.6
Na
2MoO
4·2H
2O 0.25
CuSO
4·5H
2O 0.025
CoCl
2·6H
2O 0.025
FeSO
4·7H
2O 27.8
Na
2·EDTA·2H
2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2 additional compositions:
BA 0.2-4mg/L
NAA 0.01-0.4mg/L
Agar 6000-8000mg/L
Sugar 20000-40000mg/L or
IBA 0.1-0.5mg/L
Hua Lin-43 1-5ml/L
Claims (2)
1, a kind of propagation method of the flower bud of lily magnolia the steps include:
A, flower bud of lily magnolia young sprout is cut into the stem section,, uses aseptic water washing with the sterilization of 0.1% mercury chloride;
B, again the young sprout section is cut into joint, inserts in the prepared culture medium, the unit of the following each component of MS minimal medium is a mg/litre:
NH
4NO
3 1650
KNO
3 1900
CaCl
2·2H
2O 440
MgSO
4·7H
2O 370
KH
2PO
4 170
KI 0.83
H
3BO
3 6.2
MnSO
4·4H
2O 22.3
ZnSO
4·7H
2O 8.6
Na
2MoO
4·2H
2O 0.25
CuSO
4·5H
2O 0.025
CoCl
2·6H
2O 0.025
FeSO
4·7H
2O 27.8
Na
2·EDTA·2H
2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
Additional composition:
BA 0.2-4mg/L
NAA 0.01-0.4mg/L；
C, illumination cultivation, temperature are 20-28 ℃, light intensity 1500-3000Lx, and illumination every day 10-16 hour was cultivated 7-14 days;
D, getting the tender tip, get the long tender tip of 1-5cm and be inoculated in the root media, is minimal medium according to the MS among the step B, and its additional composition is:
IBA 0.1-0.5mg/L
Hua Lin-43 1-5ml/L
2, the method for quickly breeding of a kind of flower bud of lily magnolia according to claim 1 is characterized in that the effective ingredient of medium is made up of following composition: agar 6000-8000mg/L, sugared 20000-30000mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001312693A CN1133362C (en) | 2000-12-15 | 2000-12-15 | Quick reproducing method for magnolia flower |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001312693A CN1133362C (en) | 2000-12-15 | 2000-12-15 | Quick reproducing method for magnolia flower |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1358425A CN1358425A (en) | 2002-07-17 |
| CN1133362C true CN1133362C (en) | 2004-01-07 |
Family
ID=4594590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001312693A Expired - Fee Related CN1133362C (en) | 2000-12-15 | 2000-12-15 | Quick reproducing method for magnolia flower |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1133362C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103718966B (en) * | 2013-12-25 | 2015-10-21 | 陈凤花 | A kind of magnolia liliiflora tissue culture method |
-
2000
- 2000-12-15 CN CNB001312693A patent/CN1133362C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1358425A (en) | 2002-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109618932B (en) | Method for inducing adventitious buds of rhododendron dauricum and regenerating plants | |
| CN103355174A (en) | Low-cost high-efficiency industrialized seedling production method for cold-resistant Chinese rose | |
| CN101057558A (en) | Asexual reproduction method for elaeagnus mollis | |
| CN1180671C (en) | Nutrient solution spray cuttage | |
| JPS5914725A (en) | Production of plant propagating material | |
| CN1470156A (en) | Tomato-tree cultivating method | |
| CN110663552B (en) | Tissue culture and rapid propagation method of Yunnan tung tree | |
| CN110786240B (en) | Ex-vitro rooting method for callicarpa chinensis tissue culture seedlings | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN1133362C (en) | Quick reproducing method for magnolia flower | |
| CN1418460A (en) | Method for cultivating pineapple | |
| CN102499091B (en) | Method for obtaining regeneration plants of petunia hybrida by anther culture | |
| CN107484665A (en) | A kind of method using black fruit fructus lycii resting shoot seedling | |
| CN101341846B (en) | Same-root continuous cultivation method for lianas vegetables in greenhouse | |
| CN101341833B (en) | Different-root continuous cultivation method for lianas vegetable | |
| CN1175730C (en) | Method for breeding seedlings by using tissue of lauan | |
| CN1255023C (en) | Quick breeding technolgy for Renanthera imschootiana Rolfe | |
| CN1202706C (en) | Method for production of papaya sprout with strain stabilization | |
| CN1299586A (en) | Mountain poplar tissue culture and its fast propagating culture medium | |
| CN1168378C (en) | Industrialized method for quick culturing Amorphophallus rivieri | |
| CN113170707A (en) | Efficient seedling growing method for mesona blume virus-free seedlings and application of efficient seedling growing method | |
| CN1073344C (en) | Method for propagating corms for betel nut taro seeds | |
| CN1631107A (en) | Asepsis sowing and tissue culturing technique of Cattleya bowringiana Hort. | |
| CN1271918C (en) | Quick propagation method of promising strain line of tea | |
| CN105706840A (en) | Method for improving cutting propagation survival rate of clerodendrum trichotomum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |