CN1271918C - Quick propagation method of promising strain line of tea - Google Patents
Quick propagation method of promising strain line of tea Download PDFInfo
- Publication number
- CN1271918C CN1271918C CN 01138356 CN01138356A CN1271918C CN 1271918 C CN1271918 C CN 1271918C CN 01138356 CN01138356 CN 01138356 CN 01138356 A CN01138356 A CN 01138356A CN 1271918 C CN1271918 C CN 1271918C
- Authority
- CN
- China
- Prior art keywords
- tea
- new tip
- plant
- new
- strain line
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- Expired - Fee Related
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses a rapid propagation method for excellent plant strains of tea. The method comprises the following steps: firstly, a new tip of a plant is used as an explant, and the new tip of tea is cut into sections; secondly, the sections are then disinfected by 0.1% chloromercury, and are washed by sterilized water; thirdly, the disinfected new tip is inoculated into prepared culture media; fourthly, the new tip in the culture media is cultured at a temperature of 20 to 28 DEG C and under the condition of light intensity of 1500 to 3000 Lx for 10 to 16 hours every day, so new tips come out from lateral buds; fifthly, the first step, the third step and the fourth step are repeated every 30 to 40 days for elarging propagation. The present invention has the advantages of simple and available method, convenient operation and high propagation speed. One new tip of tea can propagate 100 million strains of seedlings in one year.
Description
Technical field:
The present invention relates to the plant seedling breeding, more specifically relate to a kind of method for quickly breeding of promising strain line of tea.
Background technology:
Tealeaves belongs to Theaceae Camellia plant, comes from China, existing more than 3000 year of the history of among the people kind of tea of China, tea making.Now the world mainly produce genetic resources, cultivation step, the tea making technology of tea country and the custom of drinking tea etc. all be pass directly or indirectly from China.
Cuttage and two kinds of methods of planting seed are generally adopted in the breeding of tea tree.Cottage propagation is the main method of tealeaves breeding, is exactly clip tea tree branch, under certain conditions, is inserted in soil, sand or other matrix, makes it take root, come into leaves, and becomes complete and independent new plant.But this method, required vegetable material is a lot, the maternal plant material seldom the time its reproduction speed too slow.Use seminal propagation, the offspring separates, and can not keep maternal good characteristic.
Plant tissue culture technique belongs to biological technical field, and it is a totipotency of utilizing plant cell, and the little block organization of cultured in vitro plant carries out the industrial breeding technique of plant Clonal Rapid Propagation.It only needs plant tissue material seldom, and reproduction speed is fast, is not subject to seasonal restrictions.Under maternal plant situation seldom, can obtain a large amount of high quality seedlings in a short time with this method.
Summary of the invention:
The objective of the invention is, a kind of method for quickly breeding of promising strain line of tea is provided, this method is simple, easy to operate, reproduction speed is fast, can accelerate the quick breeding of tealeaves new varieties fine individual plant and the update of local tea variety.
In order to achieve the above object, the present invention adopts following technical measures: with the tea tree young sprout as explant, young sprout is cut into 2～3 centimetres stem section, with 0.1% chlorine mercury sterilization 5～15 minutes, with aseptic water washing 3～4 times, the young sprout that disinfects is inserted in the prepared culture medium, this medium is made up of MS minimal medium and added substance, illumination cultivation, in temperature is 20～28 ℃, and light intensity 1500～3000Lx cultivates under 10～16 hours the condition of illumination every day, make lateral bud grow young sprout, repeated above-mentioned other step of removing sterilization and flushing every 30～40 days and expand numerous.
The present invention compared with prior art has the following advantages and effect: easy to implement the method, easy to operate, reproduction speed is fast, and 30～40 days propagation multiple reaches 3～4 times, and a bud can be bred 1,000,000 strain seedlings in 1 year.
Embodiment:
The specific embodiment of the present invention is: as outer plant corpus, at first young sprout is cut into 2～3 centimetres stem section with plant tea tree young sprout, with 0.1% chlorine mercury sterilization 5～15 minutes, uses aseptic water washing 3～4 times; Then the young sprout that disinfects is inserted in the prepared culture medium, this medium is made up of MS minimal medium and added substance, and wherein the composition of added substance is that BA0.5～5mg/L, NAA0.01～0.5mg/L, agar 6000～8000mg/L, sugar 20000～30000mg/L are mixed; The 3rd is illumination cultivation, is 20～28 ℃ in temperature, light intensity 1500～3000Lx, and illumination every day was cultivated under the condition in 10～16 hours, made lateral bud grow young sprout, repeated above-mentioned other step of removing sterilization and flushing every 30～40 days and expanded numerous.
The composition of MS minimal medium be (unit: mg/L):
NH
4NO
3 1650
KNO
3 1900
CaCl
2·2H
2O 440
MgSO
4·7H
2O 370
KH
2PO
4 170
KI 0.83
H
3BO
3 6.2
MnSO
4·4H
2O 22.3
ZnSO
4·7H
2O 8.6
Na
2MoO
4·2H
2O 0.25
CuSO
4·5H
2O 0.025
CoCl
2·6H
2O 0.025
FeSO
4·7H
2O 27.8
Na
2·EDTA·2H
2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
Claims (1)
1, a kind of method for quickly breeding of promising strain line of tea, this method follow these steps to order to carry out:
A, the tea tree young sprout is cut into the stem section;
B, with 0.1% chlorine mercury sterilization, use aseptic water washing;
C, young sprout stem section is inserted in the prepared culture medium, described medium is made up of MS minimal medium and added substance, wherein:
The composition of added substance is BA0.5～5mg/L, NAA0.01～0.5mg/L, agar 6000～8000mg/L, sugar 20000～30000mg/L;
D, be 20～28 ℃ in temperature, light intensity 1500～3000Lx cultivates under 10～16 hours the condition of illumination every day;
E, every 30～40 days, the step that repeats A, C, D expands numerous.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01138356 CN1271918C (en) | 2001-12-27 | 2001-12-27 | Quick propagation method of promising strain line of tea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01138356 CN1271918C (en) | 2001-12-27 | 2001-12-27 | Quick propagation method of promising strain line of tea |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1428074A CN1428074A (en) | 2003-07-09 |
| CN1271918C true CN1271918C (en) | 2006-08-30 |
Family
ID=4674557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01138356 Expired - Fee Related CN1271918C (en) | 2001-12-27 | 2001-12-27 | Quick propagation method of promising strain line of tea |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1271918C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102422810A (en) * | 2011-09-02 | 2012-04-25 | 安徽农业大学 | In-vitro regeneration culture method for tea clones |
| CN102934603B (en) * | 2012-10-30 | 2014-07-09 | 安徽农业大学 | Asexual rapid propagation method of tea trees and root taking culture solution |
| CN103609453B (en) * | 2013-12-04 | 2015-10-14 | 福建农林大学 | A kind of construction method of tea tree vitro Regeneration System |
| CN103609454B (en) * | 2013-12-04 | 2016-03-09 | 福建农林大学 | A kind of method reducing tea tree tissue culture procedures China and foreign countries implant body pollution |
-
2001
- 2001-12-27 CN CN 01138356 patent/CN1271918C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1428074A (en) | 2003-07-09 |
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