CN100433972C - Fast propagation process of potarnogeton lucens - Google Patents
Fast propagation process of potarnogeton lucens Download PDFInfo
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- CN100433972C CN100433972C CNB2006101247632A CN200610124763A CN100433972C CN 100433972 C CN100433972 C CN 100433972C CN B2006101247632 A CNB2006101247632 A CN B2006101247632A CN 200610124763 A CN200610124763 A CN 200610124763A CN 100433972 C CN100433972 C CN 100433972C
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- milligram
- potarnogeton lucens
- lucens
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Abstract
The present invention relates to tissue culture method of aquatic plant. The fast propagation process of Potarnogeton lucens includes the following steps: 1. taking the underground stem tip as the culture; 2. washing the culture; 3. inoculating to inducing culture medium to culture; 4. transferring to differentiating and proliferating culture medium to culture; 5. further culturing the separated buds in the differentiating and proliferating culture medium to obtain great amount of Potarnogeton lucens buds, or transferring the Potarnogeton lucens buds in rooting culture medium to induce rooting and to obtain Potarnogeton lucens seedling. The present invention has optimized recipes of inducing culture medium, differentiating and proliferating culture medium and rooting culture medium, and can propagate great amount of Potarnogeton lucens seedlings used for water body vegetation.
Description
Technical field
The present invention relates to the method for tissue culture of water plants, relate in particular to a kind of potarnogeton lucens (Potamogton lucens Linn) method of breeding fast, belong to biotechnology is produced seedling in water body revegetation and water scenery layout application.
Background technology
Potarnogeton lucens belongs to the Potamogetonaceae Potamogeton, and yellowish green or band bright green is for viewing and admiring with its leaf, and than antipollution, is the important plant resources in the biological food chain, is again the main water plants in the water body revegetation.The leaf of potarnogeton lucens is oblong, ovum shape ellipse to draping over one's shoulders the needle-like ellipse, stockless or tool short handle, and handle length can reach 2 centimetres sometimes; Long 2～18 centimetres of blade, wide 0.8～3.5 centimetre, matter is thin, and tip is sharp-pointed, the aristiform tip of normal tool 0.5～2 centimeter length, the base portion wedge shape, the edge is shallow wavy, dredges and gives birth to trickle sawtooth; 5～9 of veins, life-like in clear water.
Low at the occurring in nature reproduction coefficient, as utilize conventional offshoot breeding, do not form large-scale production, be difficult to satisfy the demand of water body revegetation.
Tissue culture is as a kind of important means in the biological technical field, utilize the totipotency of plant cell, as: tissues such as stem apex, tender shoots, pass through cultured in vitro, produce callus, seedling differentiation, root induction, thereby form the plant of the Clonal Rapid Propagation of plant.Though tissue culture is a kind of technology of comparative maturity, its minimal medium generally adopts MS, to different plants, needs different nutrition configurations from the different vegetative stages that produce callus, seedling differentiation, root induction, and different growth conditionss.Therefore to the tissue culture of light leaf dish, also need special medium and condition of culture.
Summary of the invention
The purpose of this invention is to provide a kind of potarnogeton lucens method of breeding fast.
In order to achieve the above object, the present invention includes the following step:
1. get the underground stem apex of potarnogeton lucens as culture;
2. culture is disinfected;
3. insert in the inducing culture and cultivate;
4. transfer and cultivate in differentiation and proliferated culture medium;
5. with the plant division of potarnogeton lucens bud, can be used as new potarnogeton lucens culture, 4. repeating step can obtain a large amount of potarnogeton lucens buds;
With the root induction in root media of transferring of potarnogeton lucens bud, promptly obtain the potarnogeton lucens seedling.
The present invention has following advantage and good effect:
1, inducing culture of the present invention, differentiation are best suited for three different vegetative stages of potarnogeton lucens---the optimal medium prescription of inducing culture, differentiation and propagation and culture of rootage with proliferated culture medium and root media.
2, underground stem apex of potarnogeton lucens can be bred strain seedlings up to ten thousand in 1 year through propagation repeatedly, and arranging for water body revegetation and water scenery provides a large amount of seedlings.
Embodiment
Describe in detail below in conjunction with embodiment:
1, described step 1.
The underground stem apex of potarnogeton lucens of getting length and be 1～2 centimetre is as the potarnogeton lucens culture.
2, described step 2.
With the potarnogeton lucens culture with flowing water towards Xian after 30～50 minutes, take out the surface of cleaning the potarnogeton lucens culture with 75% cotton ball soaked in alcohol, use 0.1% mercury chloride soaking disinfection again 5～8 minutes, and took out with aseptic water washing 4～6 times each 2～4 minutes then;
3, described step 3.
23～25 ℃ of the temperature of cultivation, illumination 10～12h/d, intensity of illumination 1000Lx will be inserted through the potarnogeton lucens culture that 2. step is handled in the inducing culture; Cultivated 20～30 days, the potarnogeton lucens culture begins to sprout, grow;
Inducing culture is: add 0.1 milligram of KT (6-furans aminopurine), 0.1 milligram 2,4-D (2,4 dichlorophenoxyacetic acid), 30 gram sucrose, 6 gram agar in every liter of MS minimal medium;
Wherein every liter of MS minimal medium contains:
NH
4NO
3(ammonium nitrate) 1650 milligrams
KNO
3(potassium nitrate) 1900 milligrams
CaCl
22H
2440 milligrams of O (calcium chloride dihydrate)
MgSO
4.7H
2370 milligrams of O (magnesium sulfate)
KH
2PO
4(potassium dihydrogen phosphate) 170 milligrams
0.83 milligram of KI (potassium iodide)
H
3BO
3(boric acid) 6.2 milligrams
MnSO
44H
222.3 milligrams of O (manganese sulphate)
ZnSO
47H
28.6 milligrams of O (zinc sulphate)
Na
2MoO
42H
20.25 milligram of O (sodium molybdate)
CuSO
45H
20.025 milligram of O (copper sulphate)
CoCl
26H
20.025 milligram of O (cobalt chloride)
FeSO
47H
227.8 milligrams of O (ferrous sulfate)
37.3 milligrams of disodium ethylene diamine tetraacetates
100 milligrams of inositols
0.5 milligram in nicotinic acid
0.5 milligram of puridoxine hydrochloride
0.1 milligram of thiamine hydrochloride
2 milligrams of glycine
The rest is water;
4, described step 4.
To transfer in differentiation and proliferated culture medium, 25～28 ℃ of cultivation temperature, illumination 10h/d, intensity of illumination 2000Lx through the potarnogeton lucens culture that 3. step cultivates; Cultivate and grew many potarnogeton lucens buds in 30～40 days;
Differentiation with proliferated culture medium is: add 1 milligram of BA (6-benzyladenine), 0.1 milligram of NAA (methyl), 30 gram sucrose, 6 gram agar in every liter of MS minimal medium;
5, described step 5.
If with step potarnogeton lucens bud plant division 4., can be used as new potarnogeton lucens culture, 4. repeating step can obtain a large amount of potarnogeton lucens buds;
As if root induction in root media that step potarnogeton lucens bud is 4. transferred, 24～26 ℃ of cultivation temperature, illumination 12h/d, intensity of illumination 3000Lx; Cultivate and promptly obtained the potarnogeton lucens seedling in 25～30 days;
Root media is: add 1 liter of distilled water, 4 milligrams of BA (6-benzyladenine), 4 milligrams of IAA (methyl), 40 gram sucrose, 12 gram agar in every liter of MS minimal medium.
This shows, implement the present invention, can obtain a large amount of potarnogeton lucens test-tube plantlets in a short time.
Claims (1)
1, the method for the quick breeding of a kind of potarnogeton lucens is characterized in that comprising the following steps:
1. get the underground stem apex of potarnogeton lucens as culture;
2. culture is disinfected;
3. insert in the inducing culture and cultivate;
4. transfer and in differentiation and proliferated culture medium, cultivate;
5. with the plant division of potarnogeton lucens bud, as new potarnogeton lucens culture, 4. repeating step obtains a large amount of potarnogeton lucens buds;
With the root induction in root media of transferring of potarnogeton lucens bud, obtain the potarnogeton lucens seedling;
1. described step is that to get length be that 1～2 centimetre the underground stem apex of potarnogeton lucens is as the potarnogeton lucens culture;
Described step 2. be with the potarnogeton lucens culture with flowing water towards Xian after 30～50 minutes, take out the surface of cleaning the potarnogeton lucens culture with 75% cotton ball soaked in alcohol, use 0.1% mercury chloride soaking disinfection again 5～8 minutes, and took out with aseptic water washing 4～6 times each 2～4 minutes then;
3. described step is that the potarnogeton lucens culture that will 2. handle through step inserts in the inducing culture 23～25 ℃ of the temperature of cultivation, illumination 10～12h/d, intensity of illumination 1000Lx; Cultivate and formed callus in 20～30 days;
Inducing culture is: add 0.1 milligram of 6-furans aminopurine in every liter of MS minimal medium, 0.1 milligram of 2,4 dichlorophenoxyacetic acid, 30 gram sucrose and 6 gram agar;
Wherein every liter of MS minimal medium contains:
NH
4NO
31650 milligrams, KNO
31900 milligrams, CaCl
22H
2440 milligrams of O, MgSO
4.7H
2370 milligrams of O, KH
2PO
4170 milligrams, 0.83 milligram of KI, H
3BO
36.2 milligram, MnSO
44H
222.3 milligrams of O, ZnSO
47H
28.6 milligrams of O, Na
2MoO
42H
20.25 milligram of O, CuSO
45H
20.025 milligram of O, CoCl
26H
2The O0.025 milligram, FeSO
47H
227.8 milligrams of O, 37.3 milligrams of disodium ethylene diamine tetraacetates, 100 milligrams of inositols, 0.5 milligram in nicotinic acid, 0.5 milligram of puridoxine hydrochloride, 2 milligrams of 0.1 milligram of thiamine hydrochloride and glycine the rest is water;
4. described step is that the potarnogeton lucens culture of 3. cultivating through step is transferred in differentiation and proliferated culture medium, 25～28 ℃ of cultivation temperature, illumination 10h/d, intensity of illumination 2000Lx; Cultivate and grew many potarnogeton lucens buds in 30～40 days;
Differentiation with proliferated culture medium is: add 1 milligram of 6-benzyladenine in every liter of MS minimal medium, 0.1 milligram of methyl, 30 gram sucrose and 6 gram agar;
5. described step is with the root induction in root media of transferring of step potarnogeton lucens bud 4., 24～26 ℃ of cultivation temperature, illumination 12h/d, intensity of illumination 3000Lx; Cultivate and promptly obtained the potarnogeton lucens seedling in 25～30 days;
Root media is: add 1 liter of distilled water in every liter of MS minimal medium, 4 milligrams of BA, 4 milligrams of IAA, 40 gram sucrose and 12 gram agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101247632A CN100433972C (en) | 2006-10-13 | 2006-10-13 | Fast propagation process of potarnogeton lucens |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101247632A CN100433972C (en) | 2006-10-13 | 2006-10-13 | Fast propagation process of potarnogeton lucens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1930953A CN1930953A (en) | 2007-03-21 |
| CN100433972C true CN100433972C (en) | 2008-11-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006101247632A Expired - Fee Related CN100433972C (en) | 2006-10-13 | 2006-10-13 | Fast propagation process of potarnogeton lucens |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450150B (en) * | 2010-11-01 | 2013-05-22 | 南京中科水治理股份有限公司 | Propagation technology for potamogeton pectinatus L |
| TW201230951A (en) * | 2011-01-27 | 2012-08-01 | Ming-Xin Zeng | Aquatic plants indoor leaf multiplication method with leaf multiplication ability |
| CN103053422B (en) * | 2013-01-09 | 2014-03-26 | 成都华都农业发展有限责任公司 | Method for reproducing potamogeton perfoliatus |
| CN104705194A (en) * | 2015-04-03 | 2015-06-17 | 南京大学 | Hormone-induced rapid propagation method of potamogeton pectinatus engineering seedlings |
| CN106954549B (en) * | 2017-02-27 | 2019-05-28 | 水生藻安生物科技（武汉）有限公司 | A kind of comb tooth bog pondweed tissue culture and rapid proliferation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596601A (en) * | 2004-09-15 | 2005-03-23 | 中国科学院南京地理与湖泊研究所 | Method of planting |
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2006
- 2006-10-13 CN CNB2006101247632A patent/CN100433972C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596601A (en) * | 2004-09-15 | 2005-03-23 | 中国科学院南京地理与湖泊研究所 | Method of planting |
Non-Patent Citations (2)
| Title |
|---|
| 优质水生饲料-竹叶眼子菜. 裴桂秋，张睿.养殖技术顾问，第10期. 2005 * |
| 眼子菜的繁殖特性观察. 郑晓明.植物保护，第5期. 1983 * |
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| CN1930953A (en) | 2007-03-21 |
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