CN1169426C

CN1169426C - Cymbidium quick-acting breeding method

Info

Publication number: CN1169426C
Application number: CNB02139248XA
Authority: CN
Inventors: 波杨; 杨波; 龙云铭; 李洪林
Original assignee: Wuhan Institute of Botany of CAS
Current assignee: Wuhan Botanical Garden of CAS
Priority date: 2002-11-07
Filing date: 2002-11-07
Publication date: 2004-10-06
Anticipated expiration: 2022-11-07
Also published as: CN1404724A

Abstract

The present invention discloses a rapid propagation method for Chinese orchids. Top buds or lateral buds of plants are used as explants. The method comprises the steps that firstly, a plant is cut into plants of a certain length, and then the plants are soaked by 75% alcohol for a certain time, are disinfected by 0.1% mercury chloride, and are flushed by sterilized water; secondly, the disinfected buds are grafted in a prepared culture medium; thirdly, the buds are cultivated for a certain time under light irradiation every day. The method of the present invention has the advantages of easy implementation, convenient operation and high propagation speed.

Description

The method for quickly breeding of Chunlan

Technical field

The invention belongs to field of plant tissue culture technique, more specifically relate to a kind of method of Chunlan being bred fast by minimal medium and additional different components.

Background technology

Chunlan (Cymbidium goeringii (Rchb.f.) Rchb.f.) belongs to the orchid family epidendrum, is subtropical plant, originates in China, is the very abundant flowers of a kind of wild resource.But because people are in order to obtain novel treasure, one to cherish to destroy resource be cost, unauthorized and excessive mining, and it is very serious to cause wild resource to destroy, and causing of having is in imminent danger.

Offshoot is generally adopted in the breeding of Chunlan, because reproduction coefficient under field conditions (factors) is extremely low, general year reproduction coefficient is about 1, the reproduction coefficient of some famous-brand and high-quality kind even do not reach 1, therefore, though some famous-brand and high-quality kinds have been planted the history of centuries, the individual plant of depositing generation now is still very rare, make its price constantly soaring, this just copies for the malice of indivedual businessmans advantageous conditions is provided, some famous-brand and high-quality kinds have reached the personal value of millions of units, become " green stock " that everybody envies, have surmounted self due ornamental value.

Summary of the invention

The purpose of this invention is to provide the method for quickly breeding of a kind of Chunlan, easy to implement the method, easy to operate, reproduction speed is fast, can accelerate the quick breeding of the famous-brand and high-quality kind fine individual plant of Chunlan.

In order to achieve the above object, the present invention adopts following technical measures: with the terminal bud of Chunlan plant, lateral bud is as explant, at first plant is cleaned, peel off outer blade, with 75% alcohol-pickled 10～30 seconds, with 0.1% chlorine mercury sterilization 5～15 minutes, with aseptic water washing 4～5 times, the Chunlan seedling that disinfects is peelled off blade (bud), again in the MS medium that the Chunlan bud grafting is gone into to prepare, this medium comprises the material of MS minimal medium and additional different components, and wherein additional composition is 6-benzyl aminopurine (6-Benzyl aminopurine is called for short BA) 2～6mg/L, methyl (I-NaphthyIacetic acid is called for short NAA) 0.5～5mg/L, agar 6000～8000mg/L, sugar 20000～30000mg/L, coconut milk 10%, banana puree 5～10% is mixed; Illumination cultivation, temperature are 20～28 ℃, light intensity 1500～3000Lx, and illumination every day 10～16 hours was cultivated 30～90 days, grew sprouting or formed protocorm, expanded once numerous in later per 30～50 days.

The present invention compared with prior art has the following advantages and effect, and easy to implement the method, easy to operate, reproduction speed is fast, is not subject to seasonal restrictions, and 30～50 days propagation multiple reaches 2～3 times, and a bud can be bred the seedling of strain more than 500 in 1 year.

Embodiment

Embodiment 1:

As explant, at first plant is cleaned, peels off outer blade with the plant Chunlan, plant is cut into 3 centimeter length,,, use aseptic water washing 4 times with 0.1% chlorine mercury sterilization 5 minutes with 75% alcohol-pickled 30 seconds; Next is to strip out terminal bud and lateral bud, and with in the terminal bud and lateral bud access prepared culture medium that disinfect, this medium comprises the material of MS minimal medium and additional different components, and wherein additional composition is that 6-benzyl aminopurine (6-Benzyl aminopurine is called for short BA) 2mg/L, methyl (I-NaphthyIacetic acid is called for short NAA) 0.5mg/L, agar 7000mg/L, sugared 20000mg/L, coconut milk 10%, banana puree 5% are mixed; The 3rd is illumination cultivation, and temperature is 20～28 ℃, light intensity 1500～3000Lx, and illumination every day 10～16 hours was cultivated 30～90 days, made to grow sprouting, expanded once numerous in later per 30～50 days.

The composition of MS minimal medium (unit: mg/L):

Nitric acid ammonia (NH ₄NO ₃) 1650

Potassium nitrate (KNO ₃) 1900

Calcium chloride dihydrate (CaCl ₂2H ₂O) 440

Epsom salt (MgSO ₄7H ₂O) 370

Potassium dihydrogen phosphate (KH ₂PO ₄) 170

Potassium iodide (KI) 0.83

Boric acid (H ₃BO ₃) 6.2

Four water manganese sulphate (MnSO ₄4H ₂O) 22.3

White vitriol (ZnSO ₄7H ₂O) 8.6

Sodium Molybdate Dihydrate (Na ₂MoO ₄2H ₂O) 0.25

Cupric sulfate pentahydrate (CuSO ₄5H ₂O) 0.025

CoCL2 (CoCl ₂6H ₂O) 0.025

Ferrous sulfate heptahydrate (FeSO ₄7H ₂O) 27.8

Disodium ethylene diamine tetraacetate (Na ₂EDTA2H ₂O) 37.3

Inositol 100

Nicotinic acid 0.5

Puridoxine hydrochloride 0.5

Thiamine hydrochloride 0.1

Glycine 2

Embodiment 2:

As explant, at first plant is cleaned, peels off outer blade with the plant Chunlan, plant is cut into 5 centimeter length,,, use aseptic water washing 5 times with 0.1% chlorine mercury sterilization 10 minutes with 75% alcohol-pickled 20 seconds; Next is to strip out terminal bud and lateral bud, and with in the terminal bud and lateral bud access prepared culture medium that disinfect, this medium comprises the material of MS minimal medium and additional different components, wherein additional composition is BA 6mg/L, NAA 5mg/L, 2,4--dichlorphenoxyacetic acid (2,4-dichlorophenoxy aceticacid is called for short 2, and 4-D) 2mg/L, agar 7000mg/L, sugared 20000mg/L, coconut milk 10% are mixed; The 3rd is illumination cultivation, and temperature is 20～28 ℃, light intensity 1500～3000Lx, and illumination every day 10～16 hours was cultivated 60～90 days, made to grow protocorm, expanded once numerous in later per 30～50 days.

Claims

1, the method for quickly breeding of a kind of Chunlan comprises the following steps:

A, outer blade is cleaned, peelled off to plant, be cut into 3～5 centimetres, with 75% alcohol-pickled 10-30 second, with 0.1% chlorine mercury sterilization 5-15 minute, with aseptic water washing 4-5 time;

B, the Chunlan that disinfects is stripped out terminal bud and lateral bud, again the Chunlan bud grafting is gone in the prepared culture medium, the effective ingredient of medium is formed: MS minimal medium, BA2～6mg/L, NAA0.5～5mg/L, 2,4-D0.5～2mg/L, coconut milk 5～10%, banana puree 5～10%, agar 6000～8000mg/L, sugar 20000～30000mg/L;

C, illumination cultivation, temperature are 20～28 ℃, light intensity 1500～3000Lx, and illumination every day 10～16 hours was cultivated 30～90 days.