CN1169426C - Cymbidium quick-acting breeding method - Google Patents
Cymbidium quick-acting breeding method Download PDFInfo
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- CN1169426C CN1169426C CNB02139248XA CN02139248A CN1169426C CN 1169426 C CN1169426 C CN 1169426C CN B02139248X A CNB02139248X A CN B02139248XA CN 02139248 A CN02139248 A CN 02139248A CN 1169426 C CN1169426 C CN 1169426C
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- chunlan
- buds
- plant
- bud
- plants
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention discloses a rapid propagation method for Chinese orchids. Top buds or lateral buds of plants are used as explants. The method comprises the steps that firstly, a plant is cut into plants of a certain length, and then the plants are soaked by 75% alcohol for a certain time, are disinfected by 0.1% mercury chloride, and are flushed by sterilized water; secondly, the disinfected buds are grafted in a prepared culture medium; thirdly, the buds are cultivated for a certain time under light irradiation every day. The method of the present invention has the advantages of easy implementation, convenient operation and high propagation speed.
Description
Technical field
The invention belongs to field of plant tissue culture technique, more specifically relate to a kind of method of Chunlan being bred fast by minimal medium and additional different components.
Background technology
Chunlan (Cymbidium goeringii (Rchb.f.) Rchb.f.) belongs to the orchid family epidendrum, is subtropical plant, originates in China, is the very abundant flowers of a kind of wild resource.But because people are in order to obtain novel treasure, one to cherish to destroy resource be cost, unauthorized and excessive mining, and it is very serious to cause wild resource to destroy, and causing of having is in imminent danger.
Offshoot is generally adopted in the breeding of Chunlan, because reproduction coefficient under field conditions (factors) is extremely low, general year reproduction coefficient is about 1, the reproduction coefficient of some famous-brand and high-quality kind even do not reach 1, therefore, though some famous-brand and high-quality kinds have been planted the history of centuries, the individual plant of depositing generation now is still very rare, make its price constantly soaring, this just copies for the malice of indivedual businessmans advantageous conditions is provided, some famous-brand and high-quality kinds have reached the personal value of millions of units, become " green stock " that everybody envies, have surmounted self due ornamental value.
Summary of the invention
The purpose of this invention is to provide the method for quickly breeding of a kind of Chunlan, easy to implement the method, easy to operate, reproduction speed is fast, can accelerate the quick breeding of the famous-brand and high-quality kind fine individual plant of Chunlan.
In order to achieve the above object, the present invention adopts following technical measures: with the terminal bud of Chunlan plant, lateral bud is as explant, at first plant is cleaned, peel off outer blade, with 75% alcohol-pickled 10~30 seconds, with 0.1% chlorine mercury sterilization 5~15 minutes, with aseptic water washing 4~5 times, the Chunlan seedling that disinfects is peelled off blade (bud), again in the MS medium that the Chunlan bud grafting is gone into to prepare, this medium comprises the material of MS minimal medium and additional different components, and wherein additional composition is 6-benzyl aminopurine (6-Benzyl aminopurine is called for short BA) 2~6mg/L, methyl (I-NaphthyIacetic acid is called for short NAA) 0.5~5mg/L, agar 6000~8000mg/L, sugar 20000~30000mg/L, coconut milk 10%, banana puree 5~10% is mixed; Illumination cultivation, temperature are 20~28 ℃, light intensity 1500~3000Lx, and illumination every day 10~16 hours was cultivated 30~90 days, grew sprouting or formed protocorm, expanded once numerous in later per 30~50 days.
The present invention compared with prior art has the following advantages and effect, and easy to implement the method, easy to operate, reproduction speed is fast, is not subject to seasonal restrictions, and 30~50 days propagation multiple reaches 2~3 times, and a bud can be bred the seedling of strain more than 500 in 1 year.
Embodiment
Embodiment 1:
As explant, at first plant is cleaned, peels off outer blade with the plant Chunlan, plant is cut into 3 centimeter length,,, use aseptic water washing 4 times with 0.1% chlorine mercury sterilization 5 minutes with 75% alcohol-pickled 30 seconds; Next is to strip out terminal bud and lateral bud, and with in the terminal bud and lateral bud access prepared culture medium that disinfect, this medium comprises the material of MS minimal medium and additional different components, and wherein additional composition is that 6-benzyl aminopurine (6-Benzyl aminopurine is called for short BA) 2mg/L, methyl (I-NaphthyIacetic acid is called for short NAA) 0.5mg/L, agar 7000mg/L, sugared 20000mg/L, coconut milk 10%, banana puree 5% are mixed; The 3rd is illumination cultivation, and temperature is 20~28 ℃, light intensity 1500~3000Lx, and illumination every day 10~16 hours was cultivated 30~90 days, made to grow sprouting, expanded once numerous in later per 30~50 days.
The composition of MS minimal medium (unit: mg/L):
Nitric acid ammonia (NH
4NO
3) 1650
Potassium nitrate (KNO
3) 1900
Calcium chloride dihydrate (CaCl
22H
2O) 440
Epsom salt (MgSO
47H
2O) 370
Potassium dihydrogen phosphate (KH
2PO
4) 170
Potassium iodide (KI) 0.83
Boric acid (H
3BO
3) 6.2
Four water manganese sulphate (MnSO
44H
2O) 22.3
White vitriol (ZnSO
47H
2O) 8.6
Sodium Molybdate Dihydrate (Na
2MoO
42H
2O) 0.25
Cupric sulfate pentahydrate (CuSO
45H
2O) 0.025
CoCL2 (CoCl
26H
2O) 0.025
Ferrous sulfate heptahydrate (FeSO
47H
2O) 27.8
Disodium ethylene diamine tetraacetate (Na
2EDTA2H
2O) 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
Embodiment 2:
As explant, at first plant is cleaned, peels off outer blade with the plant Chunlan, plant is cut into 5 centimeter length,,, use aseptic water washing 5 times with 0.1% chlorine mercury sterilization 10 minutes with 75% alcohol-pickled 20 seconds; Next is to strip out terminal bud and lateral bud, and with in the terminal bud and lateral bud access prepared culture medium that disinfect, this medium comprises the material of MS minimal medium and additional different components, wherein additional composition is BA 6mg/L, NAA 5mg/L, 2,4--dichlorphenoxyacetic acid (2,4-dichlorophenoxy aceticacid is called for short 2, and 4-D) 2mg/L, agar 7000mg/L, sugared 20000mg/L, coconut milk 10% are mixed; The 3rd is illumination cultivation, and temperature is 20~28 ℃, light intensity 1500~3000Lx, and illumination every day 10~16 hours was cultivated 60~90 days, made to grow protocorm, expanded once numerous in later per 30~50 days.
Claims (1)
1, the method for quickly breeding of a kind of Chunlan comprises the following steps:
A, outer blade is cleaned, peelled off to plant, be cut into 3~5 centimetres, with 75% alcohol-pickled 10-30 second, with 0.1% chlorine mercury sterilization 5-15 minute, with aseptic water washing 4-5 time;
B, the Chunlan that disinfects is stripped out terminal bud and lateral bud, again the Chunlan bud grafting is gone in the prepared culture medium, the effective ingredient of medium is formed: MS minimal medium, BA2~6mg/L, NAA0.5~5mg/L, 2,4-D0.5~2mg/L, coconut milk 5~10%, banana puree 5~10%, agar 6000~8000mg/L, sugar 20000~30000mg/L;
C, illumination cultivation, temperature are 20~28 ℃, light intensity 1500~3000Lx, and illumination every day 10~16 hours was cultivated 30~90 days.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB02139248XA CN1169426C (en) | 2002-11-07 | 2002-11-07 | Cymbidium quick-acting breeding method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB02139248XA CN1169426C (en) | 2002-11-07 | 2002-11-07 | Cymbidium quick-acting breeding method |
Publications (2)
Publication Number | Publication Date |
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CN1404724A CN1404724A (en) | 2003-03-26 |
CN1169426C true CN1169426C (en) | 2004-10-06 |
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CNB02139248XA Expired - Fee Related CN1169426C (en) | 2002-11-07 | 2002-11-07 | Cymbidium quick-acting breeding method |
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Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100342778C (en) * | 2005-12-16 | 2007-10-17 | 江苏阳光生态农林开发股份有限公司 | Method for cultivating cymbidium goeringii seed by tissue culture |
CN101926259B (en) * | 2010-03-19 | 2011-12-14 | 西南林学院 | Orchid germchit propagating method |
CN101816283B (en) * | 2010-05-05 | 2011-12-07 | 浙江大学 | Method for hybridizing cymbidium goeringii, aseptically sowing seeds and raising seedlings |
CN101822220B (en) * | 2010-05-11 | 2012-01-25 | 浙江大学 | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii |
CN103314862B (en) * | 2013-07-16 | 2015-12-02 | 深圳市夺天工园林建设有限公司 | A kind of method of efficient acquisition Chunlan detoxification seedling |
CN103430842B (en) * | 2013-08-07 | 2016-06-29 | 广东省农业科学院环境园艺研究所 | A kind of Quick propagation method of hybrid orchid tissue culture |
CN104285803B (en) * | 2014-10-24 | 2016-06-29 | 柳州市天姿园艺有限公司 | Line leaf Herba Cymbidii Goeringii special culture media |
CN104285809B (en) * | 2014-10-24 | 2016-02-10 | 柳州市天姿园艺有限公司 | Cymbidium lianpan special culture media |
CN104285807B (en) * | 2014-10-24 | 2016-01-20 | 柳州市天姿园艺有限公司 | Chunlan special culture media |
CN104396741B (en) * | 2014-10-24 | 2016-08-17 | 柳州市天姿园艺有限公司 | Oecoeclades falcata special culture media |
CN104285810B (en) * | 2014-10-24 | 2016-01-20 | 柳州市天姿园艺有限公司 | Spring sword special culture media |
CN104429863B (en) * | 2014-12-17 | 2017-05-31 | 湖南省核农学与航天育种研究所 | A kind of cultural method of orchid |
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2002
- 2002-11-07 CN CNB02139248XA patent/CN1169426C/en not_active Expired - Fee Related
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