CN1171527C - Dicentra-flower quick breeding method - Google Patents
Dicentra-flower quick breeding method Download PDFInfo
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- CN1171527C CN1171527C CNB021392498A CN02139249A CN1171527C CN 1171527 C CN1171527 C CN 1171527C CN B021392498 A CNB021392498 A CN B021392498A CN 02139249 A CN02139249 A CN 02139249A CN 1171527 C CN1171527 C CN 1171527C
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- Prior art keywords
- buds
- dicentra
- lateral
- sterilized
- terminal
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses a quick breeding method for dicentra flowers, which uses terminal buds or lateral buds of plants as explants. The present invention comprises: the terminal buds or the lateral buds of the dicentra flowers are sterilized by 0.1% of mercuric chloride, and are washed by sterilized water; the sterilized terminal buds or the sterilized lateral buds are grafted in a prepared medium; the buds are cultivated under the condition of light irradiation for certain time at every day; the terminal buds or the lateral buds are grafted in the medium. The method of the present invention has the advantages of simplicity, easy operation, convenient operation and high propagation speed.
Description
Technical field
The invention belongs to field of plant tissue culture technique, more specifically relate to a kind of method of Calceolaria being bred fast by minimal medium and additional different components.
Background technology
Calceolaria (Calceolaria herbeohybrida Voss) has another name called slipperwort, belongs to Scrophulariaceae rush bag Pittosporum, originate in Mexico, Peru and Chile, happiness is nice and cool, humid climate, well-ventilated's environment, and is neither cold-resistant, also fear is hot, and the growth thermophilic is 18-25 ℃.Calceolaria is beautiful in colour, and flower shape is peculiar, is the greenhouse potted flower that is loved by the people.
Calceolaria is mainly adopted seminal propagation, but F1 costs an arm and a leg for seed, and seed is very tiny, needs low temperature to germinate, and germination rate is low, and seedling management is very difficult.
Summary of the invention
The object of the present invention is to provide the method for quickly breeding of a kind of Calceolaria, easy to implement the method, easy to operate, reproduction speed is fast, adopts tissue culture technique can breed a large amount of Calceolaria commercial seedlings fast, and can avoid torridity summer seedling stage, potted flower went on the market in advance on New Year's Day, met the need of market.
In order to achieve the above object, the present invention adopts following technical measures: with Calceolaria terminal bud or lateral bud as explant, with 0.1% mercuric chloride sterilization 5～10 minutes, use aseptic water washing again 3～4 times, the terminal bud or the lateral bud that disinfect are inserted in the prepared culture medium, this medium comprises the material of 1/2MS minimal medium and additional different components, additional different material is: 6-benzyl aminopurine (6-Benzyl aminopurine is called for short BA) 1.0～2.0mg/L, methyl (I-NaphthyIacetic acid is called for short NAA) 0.05～0.2mg/L, agar 6000～8000mg/L, sugar 20000～30000mg/L, illumination cultivation, temperature is 20～25 ℃, light intensity 2000～3000Lx, illumination every day 12～14 hours, cultivated 6～8 days, a bud or lateral bud begin growth, expand once numerous in later per 30 days.
The present invention compared with prior art, have the following advantages and effect: method is simple, and is easy to operate, reproduction speed is fast, 30 days propagation multiple reaches 3～4 times, bud can be bred 1,000,000 strain seedlings in 1 year.
Embodiment
At first be with Calceolaria terminal bud or lateral bud as explant, with 0.1% mercuric chloride sterilization 5 minutes, use aseptic water washing again 3 times; Next is that terminal bud or the lateral bud that will disinfect insert in the prepared culture medium, this medium comprises the material of 1/2MS minimal medium and additional different components, and wherein additional composition is that 6-benzyl aminopurine 1.0mg/L, methyl 0.2mg/L, agar 7000mg/L, sugared 20000mg/L are mixed; The 3rd is illumination cultivation, and temperature is 20～25 ℃, light intensity 2000～3000Lx, and illumination every day 12～14 hours was cultivated 7 days, and terminal bud or lateral bud begin growth, expand once numerous in later per 30 days.
The composition of MS minimal medium (unit: mg/L):
Nitric acid ammonia (NH
4NO
3) 1650
Potassium nitrate (KNO
3) 1900
Calcium chloride dihydrate (CaCl
22H
2O) 440
Epsom salt (MgSO
47H
2O) 370
Potassium dihydrogen phosphate (KH
2PO
4) 170
Potassium iodide (KI) 0.83
Boric acid (H
3BO
3) 6.2
Four water manganese sulphate (MnSO
44H
2O) 22.3
White vitriol (ZnSO47H2O) 8.6
Sodium Molybdate Dihydrate (Na2MoO42H2O) 0.25
Cupric sulfate pentahydrate (CuSO45H2O) 0.025
CoCL2 (CoCl26H2O) 0.025
Ferrous sulfate heptahydrate (FeSO47H2O) 27.8
Disodium ethylene diamine tetraacetate (Na
2EDTA2H
2O) 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2
Claims (1)
1, the method for quickly breeding of a kind of Calceolaria comprises the following steps:
A, with terminal bud or the lateral bud of Calceolaria, with 0.1% mercuric chloride sterilization 5～10 minutes, with aseptic water washing 3～4 times;
B, the terminal bud that disinfects or lateral bud are inserted in the prepared culture medium, the effective ingredient of medium is formed: 1/2MS minimal medium, BA1.0～2.0mg/L, NAA0.05～0.2mg/L, agar 6000～8000mg/L, sugar 20000～30000mg/L;
C, illumination cultivation, temperature are 20～25 ℃, light intensity 2000～3000Lx, and illumination every day 12～14 hours was cultivated 30 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021392498A CN1171527C (en) | 2002-11-07 | 2002-11-07 | Dicentra-flower quick breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021392498A CN1171527C (en) | 2002-11-07 | 2002-11-07 | Dicentra-flower quick breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1404725A CN1404725A (en) | 2003-03-26 |
| CN1171527C true CN1171527C (en) | 2004-10-20 |
Family
ID=4749982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021392498A Expired - Fee Related CN1171527C (en) | 2002-11-07 | 2002-11-07 | Dicentra-flower quick breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1171527C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109792952B (en) * | 2017-11-17 | 2021-12-10 | 广西壮族自治区农业科学院花卉研究所 | Method for sowing and cultivating cattail pollen |
-
2002
- 2002-11-07 CN CNB021392498A patent/CN1171527C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1404725A (en) | 2003-03-26 |
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