CN1318447C - 得自亲环蛋白b的肿瘤抗原肽 - Google Patents
得自亲环蛋白b的肿瘤抗原肽 Download PDFInfo
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- CN1318447C CN1318447C CNB998098566A CN99809856A CN1318447C CN 1318447 C CN1318447 C CN 1318447C CN B998098566 A CNB998098566 A CN B998098566A CN 99809856 A CN99809856 A CN 99809856A CN 1318447 C CN1318447 C CN 1318447C
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- tumor antigen
- antigen peptide
- peptide
- hla
- cyclophilin
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Abstract
Description
HLA-A2类型 | N末端第二个位置的氨基酸 | C末端的氨基酸 |
HLA-A0201 | L,M | V,L |
HLA-A0204 | L | L |
HLA-A0205 | V,L,I,M | L |
HLA-A0206 | V,Q | V,L |
HLA-A0207 | L | L |
腺癌细胞系 | KG-CTL产生的IFN-γ量(pg/ml) | HLA-A型 |
HT-1376(膀胱癌细胞系) | 4608 | 2402/2402 |
1-87(肺癌细胞系) | 194 | 0207/1101 |
11-18(肺癌细胞系) | 4632 | 0201/2402 |
PC-9(肺癌细胞系) | 1102 | 0206/2402 |
LC-1(肺癌细胞系) | 129 | 3101/3302 |
YT-803(肺癌细胞系) | 285 | 3101/3302 |
143B(骨肉瘤细胞系) | 1547 | 0211/0211 |
无(只有KG-CTL) | 100 | - |
细胞系 | KG-CTL产生的IFN-γ量(pg/ml) | HLA-A型 |
SSB(B细胞系1)) | 5769 | 2402/2402 |
Ban-B1(B细胞系1)) | 78 | 3101/3302 |
HPB-MLT(白血病细胞系) | 189 | 0101/0201 |
MOLT-16(白血病细胞系) | 13 | 2301/3002 |
MT-2(白血病细胞系) | 3495 | 2402/2402 |
无(只有KG-CTL) | 0 | - |
细胞 | KG-CTL产生的IFN-γ量(pg/ml) |
COS-7+HLA-A2402 | 469 |
COS-7+HLA-A2402+4F2 | 543 |
步骤 | 处理时间(分钟)×处理次数 |
1.(洗涤)DMF 1.2ml2.(去保护)50%哌定/DMF3.(洗涤)DMF 1.2ml4.(偶联)氨基保护的各氨基酸(5当量)/NMP溶液0.9ml,DIC(5当量)/NMP溶液0.3ml5.(洗涤)DMF 1.2ml6.(偶联)氨基保护的各氨基酸(5当量)/NMP溶液0.9ml,DIC(5当量)/NMP溶液0.3ml7.(洗涤)DMF 1.2ml | 1×212×11×730×11×230×11×4 |
上清液中的IFN-γ(pg/ml) | ||
抗原肽 | BEC-2 | Ban-B1 |
“84-92” | 383 | 38 |
“84-92·2F-Y” | 489 | 63 |
无 | 245 | 74 |
上清液中的IFN-γ(pg/ml) | ||
抗原肽 | BEC-2 | Ban-B1 |
“84-92” | 1896 | 160 |
“84-92·2F-Y” | 710 | 46 |
“91-99” | >2000 | 40 |
“91-99·2F-Y” | 650 | 100 |
步骤 | 处理时间(分钟)×处理次数 |
1.(洗涤)DMF 1.2ml2.(去保护)50%哌啶/DMF3.(洗涤)DMF 1.2ml4.(偶联)氨基保护的各氨基酸(5当量)/NMP溶液0.9ml,DIC(5当量)/NMP溶液0.3ml5.(洗涤)DMF 1.2ml6.(偶联)氨基保护的各氨基酸(5当量)/NMP溶液0.9ml,DIC(5当量)/NMP溶液0.3ml7.(洗涤)DMF 1.2ml | 1×212×11×730×11×230×11×4 |
对靶细胞的细胞毒性活性(%) | ||
刺激肽 | KOPT-K1 | RPMI8402 |
Cyp-A”59-67” | 27 | 0 |
Cyp-C”89-97” | 20 | 0 |
Cyp-D”94-102” | 22 | 0 |
Claims (21)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP178449/98 | 1998-06-25 | ||
JP17844998 | 1998-06-25 | ||
JP178449/1998 | 1998-06-25 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1525980A CN1525980A (zh) | 2004-09-01 |
CN1318447C true CN1318447C (zh) | 2007-05-30 |
Family
ID=16048723
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB998098566A Expired - Lifetime CN1318447C (zh) | 1998-06-25 | 1999-06-24 | 得自亲环蛋白b的肿瘤抗原肽 |
Country Status (15)
Country | Link |
---|---|
US (2) | US7041297B1 (zh) |
EP (1) | EP1090924B8 (zh) |
JP (1) | JP4413429B2 (zh) |
KR (1) | KR100653590B1 (zh) |
CN (1) | CN1318447C (zh) |
AT (1) | ATE375362T1 (zh) |
AU (1) | AU749544B2 (zh) |
CA (1) | CA2331761A1 (zh) |
DE (1) | DE69937294T2 (zh) |
ES (1) | ES2294844T3 (zh) |
HK (1) | HK1067645A1 (zh) |
ID (1) | ID27788A (zh) |
NZ (1) | NZ508996A (zh) |
TW (1) | TWI228128B (zh) |
WO (1) | WO1999067288A1 (zh) |
Families Citing this family (45)
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CA2397863A1 (en) * | 2000-01-24 | 2001-07-26 | Trustees Of Tufts College | Tetracycline compounds for treatment of cryptosporidium parvum related disorders |
US6642270B2 (en) | 2000-05-15 | 2003-11-04 | Paratek Pharmaceuticals, Inc. | 7-substituted fused ring tetracycline compounds |
WO2001098236A2 (en) * | 2000-06-16 | 2001-12-27 | Trustees Of Tufts College | 7-phenyl-substituted tetracycline compounds |
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US20080254445A1 (en) * | 2003-09-22 | 2008-10-16 | Kyogo Itoh | Prognosis in Cancer Patients Vaccinated with a Cancer Antigen Peptide-Associated Agent |
TWI261038B (en) * | 2004-08-11 | 2006-09-01 | Bo-Cheng Chen | Bicycle gear-shifting handgrip |
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TW202333769A (zh) | 2018-06-29 | 2023-09-01 | 日商大鵬藥品工業股份有限公司 | 抗腫瘤劑及其評估方法 |
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AU2020411439A1 (en) | 2019-12-26 | 2022-08-04 | Taiho Pharmaceutical Co., Ltd. | Agent for adjuvant therapy |
CN115078727A (zh) * | 2022-07-13 | 2022-09-20 | 首都医科大学附属北京胸科医院 | 一种预测癌症免疫治疗效果的标志物、试剂盒与应用 |
DE102022118517A1 (de) | 2022-07-25 | 2024-01-25 | Dennis Reitmeir | Liegestützvorrichtung mit drehbaren Griffeinheiten |
CN115372618A (zh) * | 2022-09-19 | 2022-11-22 | 首都医科大学附属北京胸科医院 | 一种检测蛋白标志物水平的试剂在制备用于评估肿瘤治疗效果的诊断产品中的应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08500106A (ja) * | 1992-08-07 | 1996-01-09 | サイテル コーポレイション | Hla結合性ペプチド及びその用途 |
JPH09151200A (ja) * | 1995-09-29 | 1997-06-10 | Ajinomoto Co Inc | ヒト胃癌に対する免疫応答を誘導できるペプチド及び該ペプチドを含むヒト胃癌治療、予防剤 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0326067A3 (en) * | 1988-01-26 | 1991-04-17 | The Du Pont Merck Pharmaceutical Company | Use of cyclophilin as an anti-inflammatory and immunomodulatory agent |
US5840839A (en) * | 1996-02-09 | 1998-11-24 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Alternative open reading frame DNA of a normal gene and a novel human cancer antigen encoded therein |
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1999
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- 1999-06-24 JP JP2000555939A patent/JP4413429B2/ja not_active Expired - Lifetime
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08500106A (ja) * | 1992-08-07 | 1996-01-09 | サイテル コーポレイション | Hla結合性ペプチド及びその用途 |
JPH09151200A (ja) * | 1995-09-29 | 1997-06-10 | Ajinomoto Co Inc | ヒト胃癌に対する免疫応答を誘導できるペプチド及び該ペプチドを含むヒト胃癌治療、予防剤 |
Also Published As
Publication number | Publication date |
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WO1999067288A1 (fr) | 1999-12-29 |
AU749544B2 (en) | 2002-06-27 |
AU4392699A (en) | 2000-01-10 |
EP1090924B8 (en) | 2007-11-21 |
TWI228128B (en) | 2005-02-21 |
HK1067645A1 (en) | 2005-04-15 |
DE69937294D1 (de) | 2007-11-22 |
ID27788A (id) | 2001-04-26 |
KR20010053180A (ko) | 2001-06-25 |
US20060008463A1 (en) | 2006-01-12 |
EP1090924A1 (en) | 2001-04-11 |
KR100653590B1 (ko) | 2006-12-04 |
WO1999067288A9 (fr) | 2000-03-30 |
US7041297B1 (en) | 2006-05-09 |
ES2294844T3 (es) | 2008-04-01 |
EP1090924B1 (en) | 2007-10-10 |
EP1090924A4 (en) | 2002-07-10 |
US7368107B2 (en) | 2008-05-06 |
CN1525980A (zh) | 2004-09-01 |
ATE375362T1 (de) | 2007-10-15 |
JP4413429B2 (ja) | 2010-02-10 |
NZ508996A (en) | 2003-09-26 |
DE69937294T2 (de) | 2008-07-03 |
CA2331761A1 (en) | 1999-12-29 |
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