CN1308442C - 纯化酶的方法和按此产生出的经纯化的酶以及该酶的用途 - Google Patents

纯化酶的方法和按此产生出的经纯化的酶以及该酶的用途 Download PDF

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Publication number
CN1308442C
CN1308442C CNB028131347A CN02813134A CN1308442C CN 1308442 C CN1308442 C CN 1308442C CN B028131347 A CNB028131347 A CN B028131347A CN 02813134 A CN02813134 A CN 02813134A CN 1308442 C CN1308442 C CN 1308442C
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CN
China
Prior art keywords
collagenase
type
chromatography
elution
clostripain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CNB028131347A
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English (en)
Chinese (zh)
Other versions
CN1529751A (zh
Inventor
M·库菲尔斯特
S·施米德鲍尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Normark Pharmaceutical Co ltd
Normark Pharmaceutical Holdings Co ltd
Original Assignee
Nordmark Arzneinmittel & CoKg GmbH
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Publication date
Priority claimed from DE10134347A external-priority patent/DE10134347B4/de
Application filed by Nordmark Arzneinmittel & CoKg GmbH filed Critical Nordmark Arzneinmittel & CoKg GmbH
Publication of CN1529751A publication Critical patent/CN1529751A/zh
Application granted granted Critical
Publication of CN1308442C publication Critical patent/CN1308442C/zh
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Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22008—Clostripain (3.4.22.8)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB028131347A 2001-07-02 2002-06-01 纯化酶的方法和按此产生出的经纯化的酶以及该酶的用途 Expired - Lifetime CN1308442C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10131994.0 2001-07-02
DE10131994 2001-07-02
DE10134347.7 2001-07-14
DE10134347A DE10134347B4 (de) 2001-07-02 2001-07-14 Verfahren zur Aufreinigung eines Enzyms

Publications (2)

Publication Number Publication Date
CN1529751A CN1529751A (zh) 2004-09-15
CN1308442C true CN1308442C (zh) 2007-04-04

Family

ID=26009625

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB028131347A Expired - Lifetime CN1308442C (zh) 2001-07-02 2002-06-01 纯化酶的方法和按此产生出的经纯化的酶以及该酶的用途

Country Status (8)

Country Link
US (1) US7083964B2 (enExample)
EP (1) EP1402008B1 (enExample)
JP (1) JP4266817B2 (enExample)
CN (1) CN1308442C (enExample)
AT (1) ATE302844T1 (enExample)
AU (1) AU2002354696A1 (enExample)
CA (1) CA2447818C (enExample)
WO (1) WO2003004628A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622562B2 (en) 2002-06-26 2009-11-24 Zimmer Orthobiologics, Inc. Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
DE10331171A1 (de) * 2003-07-09 2005-01-27 Nordmark Arzneimittel Gmbh & Co. Kg Neutrale Protease (NP) und Produkt unter Verwendung von neutraler Protease für Gewebedissoziation sowie Verfahren zu dessen Herstellung
EP2335650B1 (en) 2003-12-11 2012-10-03 Isto Technologies Inc. Particulate cartilage system
JP5292533B2 (ja) 2005-08-26 2013-09-18 ジンマー・インコーポレイテッド インプラントおよび関節疾患の治療、置換および治療方法
US20070181479A1 (en) * 2005-11-21 2007-08-09 Pentax Corporation Column and method of manufacturing the column
US7811560B2 (en) * 2006-01-30 2010-10-12 Auxilium Us Holdings, Llc Compositions and methods for treating collagen-mediated diseases
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
EP2134297B1 (en) 2007-04-12 2017-03-08 Zimmer, Inc. Apparatus for tissue repair
US10071143B1 (en) 2007-05-03 2018-09-11 The Research Foundation For The State University Of New York Methods for non-surgical treatment of carpal tunnel syndrome
US20100159564A1 (en) * 2007-11-30 2010-06-24 Dwulet Francis E Protease resistant recombinant bacterial collagenases
WO2009093255A2 (en) * 2008-01-21 2009-07-30 Ganga Raju Gokaraju A new nutraceutical composition from garcinia mangostana
EP2130551B1 (en) 2008-06-02 2012-06-06 Roche Diagnostics GmbH Improved purification of collagenases from clostridium histolyticum liquid culture
EP4772625A2 (en) 2012-01-12 2026-07-08 Endo Biologics Limited Clostridium histolyticum enzyme
US9636385B2 (en) 2012-10-24 2017-05-02 The Research Foundation For The State University Of New York Use of collagenase to treat glaucoma
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
GB201507207D0 (en) * 2015-04-24 2015-06-10 Givaudan Sa Enzymes and applications thereof
DK3589204T3 (da) 2017-03-01 2025-06-30 Endo Operations Ltd Apparat og fremgangsmåde til vurdering og behandling af cellulitis
KR20250171475A (ko) 2017-03-28 2025-12-08 엔도 오퍼레이션즈 리미티드 개선된 콜라게나제 생성 방법
EP3887386A4 (en) * 2018-11-26 2022-11-30 North Carolina State University PEPTIDE LIGANDS FOR CAPTURE OF HOST CELL PROTEINS
JP7808965B2 (ja) * 2019-02-14 2026-01-30 ノルドマルク ファーマ ゲーエムベーハー コラゲナーゼタイプｉ、コラゲナーゼタイプｉｉ、中性プロテアーゼ、およびクロストリパインからなる群から選択される少なくとも１種の酵素のクロマトグラフィー精製

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332503A (en) * 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4445891A1 (de) * 1994-12-22 1996-06-27 Boehringer Mannheim Gmbh Rekombinante Proteinase aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332503A (en) * 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase

Also Published As

Publication number Publication date
AU2002354696A1 (en) 2003-01-21
WO2003004628A3 (de) 2003-09-18
JP4266817B2 (ja) 2009-05-20
EP1402008B1 (de) 2005-08-24
CA2447818A1 (en) 2003-01-16
EP1402008A2 (de) 2004-03-31
US20040137596A1 (en) 2004-07-15
US7083964B2 (en) 2006-08-01
CA2447818C (en) 2011-01-04
CN1529751A (zh) 2004-09-15
JP2004535197A (ja) 2004-11-25
WO2003004628A2 (de) 2003-01-16
ATE302844T1 (de) 2005-09-15

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Address after: German Uetersen

Patentee after: Normark Pharmaceutical Holdings Co.,Ltd.

Address before: German Uetersen

Patentee before: NORDMARK ARZNEINMITTEL GmbH & Co.KG

CP01 Change in the name or title of a patent holder
TR01 Transfer of patent right

Effective date of registration: 20210831

Address after: German Uetersen

Patentee after: Normark Pharmaceutical Co.,Ltd.

Address before: German Uetersen

Patentee before: Normark Pharmaceutical Holdings Co.,Ltd.

TR01 Transfer of patent right
CX01 Expiry of patent term

Granted publication date: 20070404

CX01 Expiry of patent term