CN1293197C - 一种用于乳酸菌发酵的增效剂 - Google Patents
一种用于乳酸菌发酵的增效剂 Download PDFInfo
- Publication number
- CN1293197C CN1293197C CNB2004100089929A CN200410008992A CN1293197C CN 1293197 C CN1293197 C CN 1293197C CN B2004100089929 A CNB2004100089929 A CN B2004100089929A CN 200410008992 A CN200410008992 A CN 200410008992A CN 1293197 C CN1293197 C CN 1293197C
- Authority
- CN
- China
- Prior art keywords
- lactobacillus
- synergistic agent
- streptococcus
- fermented
- mol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 17
- 230000004151 fermentation Effects 0.000 title claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 36
- 241000186660 Lactobacillus Species 0.000 claims abstract description 31
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 27
- 239000012747 synergistic agent Substances 0.000 claims abstract description 27
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 32
- 235000014655 lactic acid Nutrition 0.000 claims description 16
- 241000194017 Streptococcus Species 0.000 claims description 9
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 claims description 8
- 241000194031 Enterococcus faecium Species 0.000 claims description 8
- 239000004310 lactic acid Substances 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 7
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 5
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims description 5
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 5
- 241000194032 Enterococcus faecalis Species 0.000 claims description 4
- 241001134659 Lactobacillus curvatus Species 0.000 claims description 4
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 claims description 4
- 241000186840 Lactobacillus fermentum Species 0.000 claims description 4
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 4
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 4
- 241000194034 Lactococcus lactis subsp. cremoris Species 0.000 claims description 4
- 241000192001 Pediococcus Species 0.000 claims description 4
- 241000191998 Pediococcus acidilactici Species 0.000 claims description 4
- 241000191996 Pediococcus pentosaceus Species 0.000 claims description 4
- 241000186429 Propionibacterium Species 0.000 claims description 4
- 241000186428 Propionibacterium freudenreichii Species 0.000 claims description 4
- 235000014962 Streptococcus cremoris Nutrition 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 4
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 4
- 241000186869 Lactobacillus salivarius Species 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 5
- 239000002366 mineral element Substances 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000012423 maintenance Methods 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 16
- 239000000203 mixture Substances 0.000 description 10
- 238000009472 formulation Methods 0.000 description 9
- 230000036541 health Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000006041 probiotic Substances 0.000 description 4
- 235000018291 probiotics Nutrition 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- 235000012041 food component Nutrition 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 244000005706 microflora Species 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 101710146739 Enterotoxin Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000147 enterotoxin Substances 0.000 description 1
- 231100000655 enterotoxin Toxicity 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000037345 metabolism of vitamins Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明涉及一种用于乳酸菌发酵的增效剂。该增效剂为由矿物元素Cu2+、Co2+、Mg2+、Fe2+、Zn2+、Ca2+的水溶性化合物按如下摩尔比例配成的水溶液：Cu2+∶Co2+∶Mg2+∶Fe2+∶Zn2+∶Ca2+＝1.0～6.25∶1.5～5.0∶20～100∶18～89∶38.5～115∶250～500。该增效剂在用于乳酸菌的发酵时，可补充微生物维持和生长所需的多种营养成分，明显的提高发酵液中的活菌含量，缩短发酵周期，提高生产效率，降低发酵成本，使微生态活菌制剂性能和功效更稳定、活性更强，且具有良好的经济、生态效益和社会效益的用于乳酸菌发酵的增效剂。
Description
技术领域
本发明涉及一种增效剂，具体地说是涉及一种用于乳酸菌发酵的、提高乳酸菌发酵过程中活菌生物量的增效剂。
背景技术
自50年代以来，抗生素在畜禽养殖业中的推广和应用，促进了畜牧业的迅猛的发展。但同时，人们也发现了抗生素诸如药物残留、耐药性和生态污染等一系列副面影响，危及动物或人类的公共健康。于是近年来，世界各国纷纷对抗生素采取禁用或限制措施，并积极致力于开发抗生素替代品，其中，动物微生态活菌制剂或有益微生物添加剂或统称益生素是最有代表性的一类替代物。使用微生态活菌制剂，可以促进动物生长和增重、增强机体免疫机能、降低动物死亡率，还可提高饲料转化率和动物饲养的生产效益，并且微生态活菌制剂为纯天然的生物制剂，无毒、无副作用，对动物及人类无任何不良影响。在饲料或动物饲养中应用微生态制剂，可替代抗生素或大幅度消减抗生素等药物的用量，有利于动物和人类的健康，有利于环境保护。
乳酸菌是动物消化道正常微生物区系中的优势菌群，与动物或人类“终生为伴，终生为益”，发挥如下功能：(1)拮抗病原微生物，调节动物消化道微生物区系平衡，增进动物健康；(2)乳酸菌可通过启动特异和非特异性免疫系统，增强免疫力和抗病力；(3)抑制肿瘤的发生，保护动物健康；(4)乳酸菌来治疗由大肠杆菌和沙门氏菌引发的腹泻；(5)特别是肠道中的嗜酸乳杆菌具有降低血清中胆固醇水平，防止高胆醇血症；(6)保加利亚乳杆菌的代谢产物对大肠菌释放的肠毒素有中和作用；(7)此外，乳酸菌类益生菌可以将乳中复杂的酪蛋白分解为较小的肽和氨基酸，从而降低由乳品引起的一些食物过敏反应，乳酸菌还可以提高乳糖的利用率，可以合成营养物质，产生消化酶类，提高动物消化酶的活性，改善维生素的代谢，防止便秘。因此，乳酸菌在微生态制剂中是非常有用的一类种菌。
动物微生态活菌制剂的唯一目的是为动物机体及其内环境提供有益的活菌，因此该产品中所含的活菌数量即成为影响质量的最重要的标准之一。同时，生产该类产品的发酵过程的生物量也成为影响企业经济效益的重要因素。
目前市售的微生态活菌制剂由于在发酵过程中发酵液营养成分没有达到最佳水平，结果导致：一是发酵过程的生物量(单位体积或单位重量内的菌量)较低，致使产品中的活菌数不足，严重影响了产品的质量和功效；二是导致企业生产效率不高，费时、费工、费料、费力，直接影响了企业的经济效益。因而现有的大多数微生态活菌制剂活菌数不足，达不到发挥其生理功能的要求，其使用效果的重复性和稳定性都很差，难以大面积推广和应用。
发明内容
本发明为了从根本上解决现有的微生态活菌制剂活菌数不稳定以及费时、费工、费料、费力，活菌含量达不到生理要求，其使用效果的重复性和稳定性都很差的问题，从而提供一种可补充微生物维持和生长所需的多种营养成分，明显的提高发酵液中的活菌含量，缩短发酵周期，降低发酵成本，使微生态活菌制剂性能稳定、耐加工、延长储存期、增强其生物活性，且具有良好的经济效益和社会效益的用于乳酸菌发酵的增效剂。
本发明的目的是通过如下的技术方案实现的：
本发明提供一种用于乳酸菌发酵的增效剂，其为由矿物元素Cu2+、Co2+、Mg2+、Fe2+、Zn2+、Ca2+的水溶性化合物按如下摩尔比例配成的水溶液：
Cu2+∶Co2+∶Mg2+∶Fe2+∶Zn2+∶Ca2+＝1.0～6.25∶1.5～5.0∶20～100∶18～89∶38.5～115∶250～500。
所述的Cu2+的水溶性化合物为CuSO4。
所述的Co2+的水溶性化合物为Co(NO3)2。
所述的Mg2+的水溶性化合物为MgSO4。
所述的Fe2+的水溶性化合物为FeSO4。
所述的Zn2+的水溶性化合物为ZnSO4。
所述的Ca2+的水溶性化合物为CaCl2。
所述的用于乳酸菌发酵的增效剂为由CaCl2 2～4g/L，ZnSO4 0.5～1.5g/L，FeSO40.2～1.0g/L，CuSO4 0.01～0.08g/L，Co(NO3)2 0.02～0.06g/L，MgSO4 0.1～0.5g/L组成的水溶液。
本发明提供上述用于乳酸菌发酵的增效剂在乳酸菌发酵过程中的用途。
所述的乳酸菌包括：短乳杆菌、嗜酸乳杆菌、保加利亚乳杆菌、干酪乳杆菌、纤维二糖乳杆菌、弯曲乳杆菌、德氏乳杆菌、发酵乳杆菌、乳酸乳杆菌、植物乳杆菌、植物乳杆菌、乳脂链球菌、二丁酮链球菌、粪链球菌、中间型链球菌、乳酸链球菌、嗜热链球菌、乳酸片球菌、啤酒片球菌、戊糖片球菌、谢氏丙酸杆菌、费氏丙酸杆菌、粪链球菌、屎链球菌、芽孢乳杆菌、嗜热乳杆菌、胚芽乳杆菌、唾液乳杆菌。
使用时，在发酵培养基中添加本发明提供的上述用于乳酸菌发酵的增效剂，增效剂的添加量为发酵培养基的体积百分比0.1～0.25％。
添加了本发明提供的上述用于乳酸菌发酵的增效剂，可补充微生物维持和生长所需的多种营养成分，明显的提高发酵液中的活菌含量，缩短发酵周期，降低发酵成本，使乳酸菌活菌制剂具有优秀的品质，且能产生明显的生态效益、经济效益和社会效益。
具体实施方式
实施例1、制备用于乳酸菌发酵的增效剂
将CaCl2 2～4g/L，ZnSO4 0.5～1.5g/L，FeSO4 0.2～1.0g/L，CuSO4 0.01～0.08g/L，Co(NO3)2 0.02～O.06g/L，MgSO4 0.1～0.5g/L矿物元素化合物配成液剂，各增效剂的组成和配比列于表1。
表1、
| 编号 | CaCl2(g/L) | ZnSO4(g/L) | FeSO4(g/L) | CuSO4(g/L) | Co(NO3)2(g/L) | MgSO4(g/L) |
| I | 2 | 1.5 | 0.2 | 0.08 | 0.02 | 0.5 |
| II | 2.5 | 1.1 | 0.4 | 0.05 | 0.04 | 0.4 |
| III | 3 | 0.8 | 0.7 | 0.03 | 0.05 | 0.2 |
| IV | 4 | 0.5 | 1.0 | 0.01 | 0.06 | 0.1 |
| V | 3.5 | 0.9 | 0.3 | 0.04 | 0.03 | 0.3 |
实施例2、用于乳酸菌发酵的增效剂在乳酸菌发酵中的应用
所用的乳酸菌主要包括：短乳杆菌、嗜酸乳杆菌、保加利亚乳杆菌、干酪乳杆菌、纤维二糖乳杆菌、弯曲乳杆菌、德氏乳杆菌、发酵乳杆菌、乳酸乳杆菌、植物乳杆菌、植物乳杆菌、乳脂链球菌、二丁酮链球菌、粪链球菌、中间型链球菌、乳酸链球菌、嗜热链球菌、乳酸片球菌、啤酒片球菌、戊糖片球菌、谢氏丙酸杆菌、费氏丙酸杆菌、粪链球菌、屎链球菌、芽孢乳杆菌、嗜热乳杆菌、胚芽乳杆菌、唾液乳杆菌等。
将实施例1中制备的用于乳酸菌发酵的增效剂添加到乳酸菌发酵的发酵培养基中，增效剂的添加量为发酵培养基的体积百分比0.1～0.25％，同时用不添加增效剂的发酵培养基作为对照，在同样条件下：37℃培养48h，搅拌速度230～250rpm，通气量为1～2vol/vol/min(VVM)发酵乳酸菌，结果列于表2。
所用的乳酸菌发酵液组成按重量百分比为：120目豆粕粉(0.2～1％)、120目进口鱼粉(0.5～1.0％)、120目玉米面(0.5～1.0％)、葡萄糖(2～4.5％)、NaCl(0.1～0.3％)、Na2HPO4(0.5～1.5％)、pH＝5.5～5.8。
表2、
| 种类 | 增效剂编号 | 增效剂占发酵培养液的v％ | 发酵时间 | 乳酸菌生物量 |
| 短乳杆菌对照组 | I- | 0.05- | 24h24h | 1.7×1095.0×106 |
| 嗜酸乳杆菌对照组 | III- | 0.2- | 18h18h | 3.3×1095.3×106 |
| 保加利亚乳杆菌对照组 | V- | 0.9- | 32h32h | 2.4×1093.4×108 |
| 干酪乳杆菌对照组 | II- | 0.1- | 18h18h | 1.4×1091.9×108 |
| 纤维二糖乳杆菌对照组 | IV- | 0.15- | 24h24h | 4.1×1095.4×108 |
| 弯曲乳杆菌对照组 | V- | 0.1- | 18h18h | 2.8×1095.8×108 |
| 德氏乳杆菌对照组 | IV- | 0.15- | 18h18h | 2.2×1096.1×108 |
| 发酵乳杆菌对照组 | III- | 0.06- | 18h18h | 4.5×1093.4×108 |
| 乳酸乳杆菌对照组 | I- | 0.09- | 24h24h | 2.9×1094.2×108 |
| 植物乳杆菌对照组 | III- | 0.2- | 18h18h | 1.3×1093.7×108 |
| 植物乳杆菌对照组 | IV- | 0.25- | 32h32h | 1.7×1094.5×108 |
| 乳脂链球菌对照组 | II- | 0.2- | 18h18h | 8.7×1097.4×108 |
| 二丁酮链球菌对照组 | III- | 0.19- | 18h18h | 7.6×1092.7×107 |
| 粪链球菌对照组 | IV- | 0.18- | 18h18h | 8.9×1093.5×108 |
| 中间型链球菌对照组 | V- | 0.1- | 18h18h | 4.6×1091.4×108 |
| 乳酸链球菌对照组 | I- | 0.15- | 18h18h | 2.6×10101.1×108 |
| 嗜热链球菌对照组 | II- | 0.23- | 24h24h | 3.4×1093.9×107 |
| 乳酸片球菌对照组 | III- | 0.21- | 18h18h | 2.0×1095.9×107 |
| 啤酒片球菌对照组 | V- | 0.13- | 18h18h | 1.8×1095.6×108 |
| 戊糖片球菌对照组 | I- | 0.18- | 24h24h | 5.0×1096.6×108 |
| 谢氏丙酸杆菌 | IV | 0.25 | 18h | 4.6×109 |
| 对照组 | - | - | 18h | 4.5×108 |
| 费氏丙酸杆菌对照组 | I- | 0.1- | 18h18h | 0.9×1093.2×108 |
| 粪链球菌对照组 | V- | 0.2- | 18h18h | 3.2×1098.5×107 |
| 屎链球菌、对照组 | IV- | 0.19- | 18h18h | 2.6×1097.8×107 |
| 芽孢乳杆菌对照组 | II- | 0.15- | 18h18h | 1.5×10105.4×109 |
| 嗜热乳杆菌对照组 | II- | 0.12- | 24h24h | 1.9×1095.0×107 |
| 胚芽乳杆菌对照组 | III- | 0.10- | 32h32h | 3.5×1091.4×108 |
由表2可以看出，添加了本发明提供的上述用于乳酸菌发酵的增效剂，可补充微生物维持和生长所需的多种营养成分，可使发酵液中的活菌含量提高10～100倍，因菌种而异，并缩短发酵周期，降低发酵成本，使乳酸菌活菌制剂的功效和性能更稳定、活性更强，具有良好的经济、生态效益和社会效益。
Claims (4)
1、一种用于乳酸菌发酵的增效剂，其为由CuSO4、Co(NO3)2、MgSO4、FeSO4、ZnSO4、CaCl2按如下摩尔比例配成的水溶液：
Cu2+∶Co2+∶Mg2+∶Fe2+∶Zn2+∶Ca2+＝1.0～6.25∶1.5～5.0∶20～100∶18～89∶38.5～115∶250～500。
2、如权利要求1所述的用于乳酸菌发酵的增效剂，其特征在于，所述的增效剂为由CaCl2 2～4g/L，ZnSO4 0.5～1.5g/L，FeSO4 0.2～1.0g/L，CuSO4 0.01～0.08g/L，Co(NO3)2 0.02～0.06g/L，MgSO4 0.1～0.5g/L组成的水溶液。
3、一种权利要求1或2所述的用于乳酸菌发酵的增效剂在乳酸菌发酵过程中的用途。
4、如权利要求3所述的用于乳酸菌发酵的增效剂的用途，其特征在于，所述的乳酸菌包括：短乳杆菌、嗜酸乳杆菌、保加利亚乳杆菌、干酪乳杆菌、纤维二糖乳杆菌、弯曲乳杆菌、德氏乳杆菌、发酵乳杆菌、乳酸乳杆菌、植物乳杆菌、植物乳杆菌、乳脂链球菌、二丁酮链球菌、粪链球菌、中间型链球菌、乳酸链球菌、嗜热链球菌、乳酸片球菌、啤酒片球菌、戊糖片球菌、谢氏丙酸杆菌、费氏丙酸杆菌、粪链球菌、屎链球菌、芽孢乳杆菌、嗜热乳杆菌、胚芽乳杆菌、唾液乳杆菌。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100089929A CN1293197C (zh) | 2004-03-23 | 2004-03-23 | 一种用于乳酸菌发酵的增效剂 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100089929A CN1293197C (zh) | 2004-03-23 | 2004-03-23 | 一种用于乳酸菌发酵的增效剂 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1673382A CN1673382A (zh) | 2005-09-28 |
| CN1293197C true CN1293197C (zh) | 2007-01-03 |
Family
ID=35046156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100089929A Expired - Lifetime CN1293197C (zh) | 2004-03-23 | 2004-03-23 | 一种用于乳酸菌发酵的增效剂 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1293197C (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104140946B (zh) * | 2014-07-25 | 2017-01-18 | 内蒙古斯隆生物技术有限责任公司 | 酵母菌、乳酸菌、芽孢杆菌固体发酵的增效剂 |
| CN105265994B (zh) * | 2015-10-26 | 2019-01-29 | 天津科技大学 | 一种有效提高Nisin热稳定性的方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5563051A (en) * | 1990-11-07 | 1996-10-08 | Fermentech Medical Limited | Production of hyaluronic acid |
-
2004
- 2004-03-23 CN CNB2004100089929A patent/CN1293197C/zh not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5563051A (en) * | 1990-11-07 | 1996-10-08 | Fermentech Medical Limited | Production of hyaluronic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1673382A (zh) | 2005-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102399733B (zh) | 约氏乳杆菌及其菌剂、应用和预混料 | |
| CN103766604B (zh) | 一种复合益生菌制剂的制备 | |
| CN1813562A (zh) | 复合微生物发酵饲料营养添加剂及其制备方法 | |
| CN107312732B (zh) | 一种益生菌饲料添加剂 | |
| CN101165162A (zh) | 一种益生菌活性制剂 | |
| CN101974463B (zh) | 罗伊氏乳杆菌及其复合活菌制剂 | |
| CN103184174B (zh) | 培养基中含腐植酸钠饲料用枯草芽孢杆菌生物制剂的生产 | |
| CN101756069A (zh) | 一种猪用高效环保型饲料添加剂及其制备方法 | |
| CN102028110A (zh) | 一种肉鸡饲用的益生菌-黄芪多糖组合物 | |
| CN102356829A (zh) | 生长肥育猪后期微生物发酵无抗生素饲料 | |
| CN105994941A (zh) | 一种微生物发酵制备的无抗饲料 | |
| CN102422996A (zh) | 生长肥育猪前期微生物发酵无抗生素饲料 | |
| CN106417170A (zh) | 一种应用微生态制剂和发酵豆粕的生猪生态养殖方法 | |
| CN109548955A (zh) | 一种畜禽饲料添加剂及其制备方法 | |
| CN101884394B (zh) | 一种雏鸡用复合微生物饲料添加剂及其制备方法 | |
| CN111690574A (zh) | 一株类肠膜魏斯氏菌及其应用 | |
| CN106906166A (zh) | 一种猪源唾液乳杆菌活菌制剂及应用 | |
| CN112280707A (zh) | 一种饲料用益生菌的制备方法 | |
| CN105851485A (zh) | 一种液态复合益生菌制剂及其制备方法 | |
| WO2011078213A1 (ja) | プロピオン酸菌生菌含有組成物、及びその利用方法 | |
| RU2742866C1 (ru) | Кормовая пробиотическая добавка для крупного рогатого скота | |
| CN1322113C (zh) | 一种芽孢乳杆菌及其生产的活菌制剂 | |
| CN1293197C (zh) | 一种用于乳酸菌发酵的增效剂 | |
| CN103211085A (zh) | 一种饲用高效复合益生菌剂及制备方法 | |
| CN101623048A (zh) | 一种富钾复合益生素饲料添加剂 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING GINTEN BIOTECH. CO., LTD. Free format text: FORMER OWNER: CHINA AGRICULTURAL UNIVERSITY Effective date: 20111020 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100094 HAIDIAN, BEIJING TO: 102206 CHANGPING, BEIJING |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20111020 Address after: 102206, room 18, Fukang Road, Changping District science and Technology Park, Beijing, 628 Patentee after: BEIJING GINTEN BIOTECH Co.,Ltd. Address before: 100094 Beijing Old Summer Palace West Road, Haidian District, No. 2 Patentee before: China Agricultural University |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Synergist for fermentation of lactolin Effective date of registration: 20130122 Granted publication date: 20070103 Pledgee: Industrial Commercial Bank of China Ltd. Beijing Haidian West Branch Pledgor: BEIJING GINTEN BIOTECH Co.,Ltd.|Zhang Shixin Registration number: 2013990000048 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20140114 Granted publication date: 20070103 Pledgee: Industrial Commercial Bank of China Ltd. Beijing Haidian West Branch Pledgor: BEIJING GINTEN BIOTECH Co.,Ltd.|Zhang Shixin Registration number: 2013990000048 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Synergist for fermentation of lactolin Effective date of registration: 20151010 Granted publication date: 20070103 Pledgee: Zhongguancun Beijing technology financing Company limited by guarantee Pledgor: BEIJING GINTEN BIOTECH Co.,Ltd. Registration number: 2015990000855 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 102206, room 18, Fukang Road, Changping District science and Technology Park, Beijing, 628 Patentee after: GINTEN BIOTECHNOLOGY (BEIJING) Co.,Ltd. Address before: 102206, room 18, Fukang Road, Changping District science and Technology Park, Beijing, 628 Patentee before: BEIJING GINTEN BIOTECH Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20070103 |